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MXPA05010478A - Procedimiento para la tincion de esperma. - Google Patents

Procedimiento para la tincion de esperma.

Info

Publication number
MXPA05010478A
MXPA05010478A MXPA05010478A MXPA05010478A MXPA05010478A MX PA05010478 A MXPA05010478 A MX PA05010478A MX PA05010478 A MXPA05010478 A MX PA05010478A MX PA05010478 A MXPA05010478 A MX PA05010478A MX PA05010478 A MXPA05010478 A MX PA05010478A
Authority
MX
Mexico
Prior art keywords
sperm
cells
sperm cells
staining
involve
Prior art date
Application number
MXPA05010478A
Other languages
English (en)
Inventor
Jeannet A Glaenzer
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of MXPA05010478A publication Critical patent/MXPA05010478A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0608—Germ cells
    • C12N5/0612—Germ cells sorting of gametes, e.g. according to sex or motility
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Reproductive Health (AREA)
  • Endocrinology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

Las celulas de esperma se tinen de conformidad con procedimientos que implican la combinacion de celulas de esperma con un colorante selectivo de ADN fluorescente a una temperatura elevada en exceso de aproximadamente 40°C; los metodos permiten un tiempo de tincion disminuido; las celulas pueden de aqui en adelante clasificarse eficazmente de conformidad con metodos de separacion comunes, incluyendo citometria de flujo.
MXPA05010478A 2003-03-28 2004-03-29 Procedimiento para la tincion de esperma. MXPA05010478A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US45873103P 2003-03-28 2003-03-28
PCT/US2004/009903 WO2004087177A1 (en) 2003-03-28 2004-03-29 Process for the staining of sperm

Publications (1)

Publication Number Publication Date
MXPA05010478A true MXPA05010478A (es) 2005-12-15

Family

ID=33131822

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA05010478A MXPA05010478A (es) 2003-03-28 2004-03-29 Procedimiento para la tincion de esperma.

Country Status (14)

Country Link
US (1) US7335507B2 (es)
EP (1) EP1608382B1 (es)
JP (1) JP2006525019A (es)
CN (1) CN100379429C (es)
AT (1) ATE369867T1 (es)
AU (1) AU2004226343B2 (es)
BR (1) BRPI0408852A (es)
CA (1) CA2519989A1 (es)
DE (1) DE602004008241T2 (es)
MX (1) MXPA05010478A (es)
NZ (1) NZ542688A (es)
PL (1) PL1608382T3 (es)
WO (1) WO2004087177A1 (es)
ZA (1) ZA200507823B (es)

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JP4323571B2 (ja) 1997-01-31 2009-09-02 エックスワイ， インコーポレイテッド 光学装置
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DK1100534T3 (da) 1998-07-30 2008-03-10 Xy Inc System til ikke-kirurgisk kunstig insemination af heste
US7208265B1 (en) * 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
HUP0302232A2 (hu) 2000-05-09 2003-10-28 Xy, Inc. Berendezés és eljárás X-kromoszómát hordozó hímivarsejtek és Y-kromoszómát hordozó hímivarsejtek szétválogatására, eljárás részecskék megkülönböztetésére és részecskeosztályozó berendezés
CA2411462A1 (en) * 2000-06-12 2001-12-20 Colorado State University Research Foundation Integrated herd management system utilizing isolated populations of x-chromosome bearing and y-chromosome bearing spermatozoa
US20040031071A1 (en) * 2000-10-05 2004-02-12 Xy, Inc. System of hysteroscopic insemination of mares
US7713687B2 (en) 2000-11-29 2010-05-11 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
US7094527B2 (en) * 2000-11-29 2006-08-22 Xy, Inc. System for in-vitro fertilization with spermatozoa separated into X-chromosome and Y-chromosome bearing populations
US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
EP2283724B8 (en) 2002-08-01 2016-03-16 Xy, Llc Heterospermic insemination to assess sperm function
MXPA05001654A (es) * 2002-08-15 2005-10-18 Xy Inc Citometro de flujo de alta resolucion.
US7169548B2 (en) 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
EP3511693B1 (en) 2003-03-28 2022-08-24 Inguran, LLC Apparatus for detecting the breakoff point of a droplet generation system
PL1608382T3 (pl) 2003-03-28 2008-03-31 Inguran Llc Sposób barwienia spermy
US20060263829A1 (en) * 2003-05-15 2006-11-23 Evans Kenneth M Efficient haploid cell sorting flow cytometer systems
DE602005018907D1 (de) * 2004-03-29 2010-03-04 Inguran Llc Verwendung einer oxidations-/reduktionsreaktionen intrazellulär und/oder extrazellulär regulierenden zusammensetzung in einem anfärbe- oder sortierungsverfahren für spermatozoen
EP1730266A2 (en) * 2004-03-29 2006-12-13 Monsanto Technology, LLC Sperm suspensions for sorting into x or y chromosome-bearing enriched populations
WO2006012597A2 (en) 2004-07-22 2006-02-02 Monsanto Technology Llc Process for enriching a population of sperm cells
US7355696B2 (en) 2005-02-01 2008-04-08 Arryx, Inc Method and apparatus for sorting cells
US7618770B2 (en) 2005-07-29 2009-11-17 Xy, Inc. Methods and apparatus for reducing protein content in sperm cell extenders
KR100949923B1 (ko) * 2008-04-21 2010-03-30 중앙대학교 산학협력단 번식능력이 불량한 수퇘지 선별법
US20110236923A1 (en) * 2010-03-23 2011-09-29 Genetics & Ivf Institute Method for staining and sorting of a small volume of sperm
WO2011123166A2 (en) * 2010-04-01 2011-10-06 Inguran, Llc Methods and systems for reducing dna fragmentation in a processed sperm sample
CN101793641B (zh) * 2010-04-14 2011-09-28 内蒙古蒙牛繁育生物技术股份有限公司 一种用于奶牛精子分离的荧光染色方法
CN102095675B (zh) * 2010-11-23 2012-05-23 内蒙古赛科星繁育生物技术股份有限公司 混合奶牛性控冻精产品鉴定和检测方法
US9781919B2 (en) 2011-06-01 2017-10-10 Inguran, Llc Compositions and methods for improving the quality of processed sperm
US9347038B2 (en) 2011-06-01 2016-05-24 Inguran, Llc Compositions and methods for processing sperm
US9581587B2 (en) 2011-09-30 2017-02-28 Inguran, Llc Sperm staining and sorting methods
US9433195B2 (en) 2012-06-06 2016-09-06 Inguran, Llc Methods for increasing genetic progress in a line or breed of swine using sex-selected sperm cells
US9888990B2 (en) 2012-06-06 2018-02-13 Inguran, Llc Methods for use of sex sorted semen to improve genetic management in swine
WO2014055112A1 (en) 2012-10-05 2014-04-10 Inguran, Llc High efficiency methods of sex sorting sperm
US10620213B2 (en) 2012-10-05 2020-04-14 Inguran, Llc High pressure sperm sorting and flow cytometer methods
CN103344543B (zh) * 2013-06-28 2016-07-06 浙江星博生物科技股份有限公司 一种基于流式细胞术的精子线粒体膜电位检测试剂
CN103335934A (zh) * 2013-06-28 2013-10-02 浙江星博生物科技有限公司 一种基于流式细胞术的精子活率检测试剂
CN103555662B (zh) * 2013-10-31 2015-09-16 大连金弘基种畜有限公司 将四溴化1,1,2,2-四-[4-(2-三乙基乙氧基胺基)]苯乙烯应用于精子分离
CN104749144A (zh) * 2013-12-31 2015-07-01 深圳迈瑞生物医疗电子股份有限公司 血细胞检测试剂及血细胞处理方法和识别方法
US11413358B2 (en) 2018-04-09 2022-08-16 Inguran, Llc Sperm nuclei and methods of their manufacture and use
US10603075B1 (en) 2018-11-30 2020-03-31 Ohana Biosciences, Inc. Compositions and methods for enhancing sperm function
JP2023547605A (ja) * 2020-10-15 2023-11-13 エービーエス グローバル インコーポレイテッド ブタ雌雄鑑別精液及び使用方法

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US4474875A (en) 1969-04-10 1984-10-02 Wallace Shrimpton Method and means for controlling the sex of mammalian offspring and product therefor
US3791384A (en) 1971-07-15 1974-02-12 Schaumann H Artificial insemination of sows
US4267268A (en) 1979-03-12 1981-05-12 Nelson Jr Robert A Spermatozoa extenders
US4362246A (en) * 1980-07-14 1982-12-07 Adair Edwin Lloyd Method of treating collected mammal semen and separating sperm into X Y components
GB2145112B (en) 1983-04-27 1987-02-18 Milk Marketing Board Sorting living spermatozoa
CA2055494C (en) * 1989-05-10 2000-01-18 Lawrence Arthur Johnson Method to preselect the sex of offspring
US5658751A (en) * 1993-04-13 1997-08-19 Molecular Probes, Inc. Substituted unsymmetrical cyanine dyes with selected permeability
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US5985216A (en) * 1997-07-24 1999-11-16 The United States Of America, As Represented By The Secretary Of Agriculture Flow cytometry nozzle for high efficiency cell sorting
AU9247598A (en) * 1997-09-22 1999-04-12 University Of Guelph Reduction of sperm sensitivity to chilling
US6071689A (en) 1997-12-31 2000-06-06 Xy, Inc. System for improving yield of sexed embryos in mammals
US6149867A (en) * 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
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US6309815B1 (en) 1998-08-07 2001-10-30 University Of Kansas Medical Center Composition and method for preparation, storage and activation of large populations of immotile sperm
WO2000054026A1 (en) 1999-03-05 2000-09-14 Hatting-Ks Determination of sperm concentration and viability for artificial insemination
FR2793495B1 (fr) * 1999-05-14 2001-08-10 Imv Technologies Dilueur pour la cryoconservation de spermatozoides de bovins
US7208265B1 (en) 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
US6263745B1 (en) * 1999-12-03 2001-07-24 Xy, Inc. Flow cytometer nozzle and flow cytometer sample handling methods
IT1317724B1 (it) 2000-01-14 2003-07-15 Istituto Sperimentale Italiano Procedimento per la produzione di embrioni non umani di sessopredeterminato ad alto valore genetico.
HUP0302232A2 (hu) 2000-05-09 2003-10-28 Xy, Inc. Berendezés és eljárás X-kromoszómát hordozó hímivarsejtek és Y-kromoszómát hordozó hímivarsejtek szétválogatására, eljárás részecskék megkülönböztetésére és részecskeosztályozó berendezés
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CA2454340A1 (en) 2000-11-22 2002-05-30 Pharmacia Corporation Methods and apparatus for producing gender enriched sperm
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WO2002077011A2 (en) * 2001-03-12 2002-10-03 The State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Oxidation-reduction sensitive green fluorescent protein variants
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PL1608382T3 (pl) 2003-03-28 2008-03-31 Inguran Llc Sposób barwienia spermy

Also Published As

Publication number Publication date
EP1608382A1 (en) 2005-12-28
BRPI0408852A (pt) 2006-04-04
CA2519989A1 (en) 2004-10-14
PL1608382T3 (pl) 2008-03-31
EP1608382B1 (en) 2007-08-15
AU2004226343B2 (en) 2008-10-09
US20050003472A1 (en) 2005-01-06
AU2004226343A1 (en) 2004-10-14
WO2004087177A1 (en) 2004-10-14
ATE369867T1 (de) 2007-09-15
US7335507B2 (en) 2008-02-26
JP2006525019A (ja) 2006-11-09
ZA200507823B (en) 2008-03-26
NZ542688A (en) 2008-06-30
CN1795004A (zh) 2006-06-28
CN100379429C (zh) 2008-04-09
DE602004008241T2 (de) 2008-05-08
DE602004008241D1 (de) 2007-09-27

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