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MXPA05000190A - Metodo para detectar polinucleotidos mutados dentro de una gran poblacion de polinucleotidos de tipo silvestre. - Google Patents

Metodo para detectar polinucleotidos mutados dentro de una gran poblacion de polinucleotidos de tipo silvestre.

Info

Publication number
MXPA05000190A
MXPA05000190A MXPA05000190A MXPA05000190A MXPA05000190A MX PA05000190 A MXPA05000190 A MX PA05000190A MX PA05000190 A MXPA05000190 A MX PA05000190A MX PA05000190 A MXPA05000190 A MX PA05000190A MX PA05000190 A MXPA05000190 A MX PA05000190A
Authority
MX
Mexico
Prior art keywords
polynucleotides
wild
type
target sequence
mutant
Prior art date
Application number
MXPA05000190A
Other languages
English (en)
Inventor
Baochuan Guo
Original Assignee
Univ State Cleveland
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ State Cleveland filed Critical Univ State Cleveland
Publication of MXPA05000190A publication Critical patent/MXPA05000190A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Pathology (AREA)
  • Hospice & Palliative Care (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Se proporcionan metodos para detectar un polinucleotido mutante en mezcla con polinucleotidos mutantes, polinucleotidos de tipo silvestre y polinucleotidos no relacionados; el metodo utiliza un iniciador de extension complementario a una primera secuencia objetivo tanto en los polinucleotidos de tipo silvestre como en los polinucleotidos mutantes; el metodo tambien utiliza una sonda complementaria a una segunda secuencia objetivo en los polinucleotidos de tipo silvestre, pero no en los polinucleotidos mutantes; la extension de los iniciadores unidos a la primera secuencia objetivo en los polinucleatidos mutantes produce productos de la larga extension; la extension de los iniciadores unidos a la primera secuencia objetivo en los polinucleotidos de tipo silvestre es bloqueada por la sonda que esta unida a la segunda secuencia objetivo; se producen productos de corta extension o productos sin extension; los productos de extension se aislan y se utilizan en una reaccion en cadena de polimerasa (PCR); la PCR amplifica preferencialmente los productos de larga extension.
MXPA05000190A 2002-07-01 2003-07-01 Metodo para detectar polinucleotidos mutados dentro de una gran poblacion de polinucleotidos de tipo silvestre. MXPA05000190A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US39225102P 2002-07-01 2002-07-01
PCT/US2003/020768 WO2004003173A2 (en) 2002-07-01 2003-07-01 Method for detecting mutated polynucleotides within a large population of wild-type polynucleotides

Publications (1)

Publication Number Publication Date
MXPA05000190A true MXPA05000190A (es) 2005-09-30

Family

ID=30000834

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA05000190A MXPA05000190A (es) 2002-07-01 2003-07-01 Metodo para detectar polinucleotidos mutados dentro de una gran poblacion de polinucleotidos de tipo silvestre.

Country Status (6)

Country Link
US (2) US20040091905A1 (es)
EP (1) EP1558759A4 (es)
AU (1) AU2003280297A1 (es)
CA (1) CA2491563A1 (es)
MX (1) MXPA05000190A (es)
WO (1) WO2004003173A2 (es)

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GB0406863D0 (en) * 2004-03-26 2004-04-28 Qiagen As Nucleic acid sequencing
DE102004034343B4 (de) * 2004-07-10 2007-08-30 Olfert Landt Verfahren zum Nachweis von Spuren genomischer Varianten mittels Nukleinsäure-Amplifikationsreaktionen
AU2007249812B2 (en) 2006-05-12 2013-08-15 Cepheid DNA recombination junction detection
US7803543B2 (en) * 2007-01-19 2010-09-28 Chang Gung University Methods and kits for the detection of nucleotide mutations using peptide nucleic acid as both PCR clamp and sensor probe
GB0703996D0 (en) * 2007-03-01 2007-04-11 Oxitec Ltd Nucleic acid detection
GB0703997D0 (en) * 2007-03-01 2007-04-11 Oxitec Ltd Methods for detecting nucleic sequences
GB201100150D0 (en) 2011-01-06 2011-02-23 Epistem Ltd Mutational analysis
WO2012095639A2 (en) 2011-01-14 2012-07-19 Genefirst Limited Methods, compositions, and kits for determing the presence/absence of a varian nucleic acid sequence
AU2012214312A1 (en) 2011-02-09 2013-08-22 Bio-Rad Laboratories, Inc. Analysis of nucleic acids
CN102242211A (zh) * 2011-07-08 2011-11-16 无锡锐奇基因生物科技有限公司 检测突变核酸序列的方法及试剂盒
WO2014070540A1 (en) * 2012-11-01 2014-05-08 Siemens Healthcare Diagnostics Inc. Sequencing-based quantification of nucleic acid targets
EP3024948B1 (en) * 2013-07-25 2020-01-15 Bio-rad Laboratories, Inc. Genetic assays for detecting viral recombination rate
CN110621784B (zh) * 2017-03-24 2024-06-11 海阳生物材料有限公司 使用双功能pna探针诊断微卫星不稳定性的方法和诊断微卫星不稳定性的试剂盒
CA3224410A1 (en) * 2021-07-09 2023-01-12 Henrik Eriksen A primer

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9211979D0 (en) * 1992-06-05 1992-07-15 Buchard Ole Uses of nucleic acid analogues
US5843756A (en) * 1995-06-07 1998-12-01 Myriad Genetics, Inc. Mouse MTSI gene
US5830712A (en) * 1996-02-06 1998-11-03 Allelix Biopharmaceuticals Inc. Selective template deletion method
US6630301B1 (en) * 1997-03-14 2003-10-07 The Penn State Research Foundation Detection of extracellular tumor-associated nucleic acid in blood plasma or serum
US6492109B1 (en) * 1997-09-23 2002-12-10 Gene Logic, Inc. Susceptibility mutation 6495delGC of BRCA2
US6503718B2 (en) * 1999-01-10 2003-01-07 Exact Sciences Corporation Methods for detecting mutations using primer extension for detecting disease
US6280947B1 (en) * 1999-08-11 2001-08-28 Exact Sciences Corporation Methods for detecting nucleotide insertion or deletion using primer extension
US6552335B1 (en) * 2000-06-13 2003-04-22 Cleveland State University SDIFA mass spectrometry
US6844152B1 (en) * 2000-09-15 2005-01-18 Promega Corporation Detection of microsatellite instability and its use in diagnosis of tumors
US6479242B1 (en) * 2000-10-26 2002-11-12 Cleveland State University Method for genotyping of single nucleotide polymorphism
US20030211483A1 (en) * 2002-05-09 2003-11-13 Schroeder Benjamin G. Methods for the enrichment of low-abundance polynucleotides
US20040096870A1 (en) * 2002-06-28 2004-05-20 Sention, Inc. Methods of detecting sequence differences

Also Published As

Publication number Publication date
EP1558759A4 (en) 2007-06-06
US20040091905A1 (en) 2004-05-13
AU2003280297A1 (en) 2004-01-19
AU2003280297A8 (en) 2004-01-19
EP1558759A2 (en) 2005-08-03
WO2004003173A3 (en) 2004-06-24
US20070207494A1 (en) 2007-09-06
WO2004003173A2 (en) 2004-01-08
CA2491563A1 (en) 2004-01-08

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