MXPA99009864A - System and method for the cosmetic care of lap - Google Patents

Abstract

A method for the cosmetic care of the skin based on the application of the skin of a combination of the South pole of a static magnet and a cosmetic composition. Also described is a double cosmetic patch comprising a static magnet layer and a non-woven, transdermal patch of hydrogel or silicone laminate that conveys a beneficial cosmetic ingredient, wherein the two layers are adhesively bonded between

Description

SYSTEM AND "METHOD FOR COSMETIC SKIN CARE FIELD OF THE INVENTION The present invention relates to a system and method for cosmetic skin care comprising a combination of a static magnet and a hydroxy acid.

BACKGROUND OF THE INVENTION Magnetic fields generated by electric currents have been implicated in many disorders including cancer. In contrast, static magnetic fields are considered safe and have been used medicinally for the treatment of pain and for the stimulation of circulation. Static magnets (also known as "permanent magnets") come in different sizes and strengths. They can be attached to the human body by strips, bands or ribbons. In a cosmetic field, compositions containing magnetized particles, for example barium hexaferrite, have been described. The summary of the 1996 conference by K. Stanzl describes that such compositions decrease the number of folds in the skin and increase the measured firmness of the skin and the level of moisture.

See also Happi, March 1996 from the article "A Safe Effective Cellulite Treatmen" which describes the use of a skin cream containing magnetized crystals of barium hexaferrite for cellulite reduction. The German patent application DE 4325071 (for Lancaster) describes the use of magnetized particles incorporated in a cosmetic base with perfluorocarbons in phospholipid vesicles to stimulate blood circulation in the skin and improve hair growth. The composition can be in the form of soap, cream, gel, etc. in a union, in a dressing or sprinkling. The composition may include antioxidants. It is considered that the magnetized particles included in cosmetic compositions have a random orientation. The application for Spanish patent ES 2099685 (for Eidos SRL) describes a magnetized cosmetic preparation which contains a magnetic element in the form of a tablet, which produces a magnetic field of up to 12,000 Gauss (1.2 Teslas in S.I. units). The application for German patent DE 3613280 (for Rehnmagnet Horst Baermann GmbH) describes a cosmetic facial mask containing small permanent magnets (0.5-30 mm). The mask also contains substances which affirm or make the skin more uniform, for example plant or animal extracts. It is stated that these substances can penetrate the skin better and that their action improves due to heating and better blood flow resulting from the use of magnets. The magnets can have two poles or multiple alternating polarity. Such static concentric circular magnets contain alternating poles and are sold by BlOflex to reduce facial wrinkles. The prior art described above does not disclose any cosmetic method for skin care wherein a south pole of a permanent magnet and a cosmetic active substance are applied to the skin. Similarly, the prior art does not disclose a cosmetic double patch consisting of a permanent magnet layer and a cosmetic ingredient layer, the latter preferably being a transdermal or hydrogel patch.

BRIEF DESCRIPTION OF THE INVENTION The invention includes, in its first embodiment (A) a cosmetic method for treating the skin, the method comprising: (a) applying to the skin the south pole of a static magnet containing at least one north pole and one south pole; and (b) applying to the skin a composition for cosmetic skin care comprising a cosmetic beneficial ingredient in a cosmetically acceptable vehicle.

According to the method of the invention, the magnet and the composition can be applied concurrently or sequentially. In its second embodiment (B), the invention also includes a double cosmetic patch comprising: (i) a static magnet layer; (ii) a layer of cosmetic ingredient which carries a cosmetic active substance; wherein the two layers are joined together and the south pole of the layer (i) is oriented towards the layer (ii).

DETAILED DESCRIPTION OF THE INVENTION Except in the operation and comparison examples, or where otherwise explicitly stated, all numbers in the description indicate material quantities or reaction conditions, physical properties of materials and / or use that should be understood as modified by the word "approximately". All amounts are by weight of the oil-in-water emulsion, unless otherwise specified. The term "skin" as used herein includes the skin of the face, neck, chest, back, legs, arms, hands and scalp.

MODALITY A: METHOD FOR COSMETIC SKIN CARE The method for the cosmetic skin care of the invention involves the application of a permanent magnet to the skin of the south pole. The magnets included in the present invention can have any shape or configuration. Preferred are bar magnets (with a north pole and a south pole). The magnet can be applied to the skin with the help of a strip or a plaster or a tape, or an adhesive. Preferably, the thickness of the magnet is at least 1.0 cm, more preferably at least 1.5 cm, in order to maximize the cosmetic benefit that is obtained with the method according to the invention. The typical strength of the magnets suitable for the present invention is in the range from 0.00004 to 0.2 Teslas, preferably from 0.01 to 0.1 Teslas. The method for cosmetic skin care according to the present invention includes applying to the skin a cosmetic composition containing a cosmetic benefit ingredient. The cosmetic benefit ingredient is preferably selected from hydroxy acids, retinoids, vitamin C, phytic acid and skin depigmenting agents, such as yombin and kojic acid. Hydroxycarboxylic acids improve the proliferation and increase the biosynthesis of ceramide in keratinocytes, increase the epidermal thickness and increase the desquamation of the normal skin "resulting in a more uniform skin, with a more youthful appearance." The hydroxy acid can be chosen from alpha -hydroxy acids, beta-hydroxy acids (for example salicylic acid), other hydroxycarboxylic acids (for example dihydroxycarboxylic acid), hydroxy-dicarboxylic acids, hydroxytricarboxylic acids) and mixtures thereof, or a combination of their stereoisomers (DL, D or L). , the alpha-hydroxycarboxylic acid is chosen from alpha-hydroxy acids having the general structure (1): OH MCHCOOH (1) wherein M is H or a linear or branched saturated or unsaturated hydrocarbon chain containing from 1 to 27 carbon atoms. Even more preferably, the hydroxycarboxylic acid is selected from lactic acid, 2-hydroxyoctanoic acid, hydroxylauric acid, glycolic acid, salicylic acid and mixtures thereof. When stereoisomers are present, the most preferred is the L-isomer. It should be understood that depending on the pH of the composition, the hydroxy acid may be present as a salt, for example an ammonium, potassium or sodium salt.

Certain hydroxy acid esters, and especially the ^ - ^ salicylic acid esters have anti-aging activity and / or oil control and can be included in the composition. A particularly preferred ester is tridecyl salicylate. Preferably, the amount of hydroxy acid component present is from 0.01 to 20%, more preferably from 0.05 to 10%, and most preferably from 0.1 to 3% by weight. The cosmetic composition preferably comprises vitamins which are selected from the group consisting of vitamin A compounds (retinoids) and vitamin C compounds. Retinoids improve the proliferation of keratinocytes in vitro, increase epidermal thickness and increase the synthesis of collagen by dermal fibroblasts . This results in damage protection and smoothing of wrinkled skin. The term "retinoids" as used herein includes retinoic acid, retinol, retinal and retinyl esters of C2-C20. Within the term "retinoic acid" is 13-cis retinoic acid and all-trans retinoic acid. The term "retinol" includes the following isomers of retinol: all-trans retinol, 13-cis retinol, 11-cis retinol, 9-cis retinol, 3,4-dideshidrorretinol. The preferred isomers are all-trans retinol, 13-cis retinol, 3,4-dideshidrorretinol and 9-cis retinol. The most preferred is all-trans retinol, due to its wide commercial availability.

"Retinyl ester is a retinyl ester The term" retinol "has been defined above The retinyl esters suitable for use in the present invention are C2-C20 retinol esters Examples of retinyl esters include, but are not limited to: retinyl palmitate, retinyl formate, retinyl acetate, retinyl propionate, retinyl butyrate, retinyl valerate, retinyl isovalerate, retinyl hexanoate, retinyl heptanoate, retinyl octanoate, retinyl nonanoate, retinyl decanoate , retinyl undecanoate, retinyl laurate, retinyl tridecanoate, retinyl myristate, retinyl pentadecanoate, retinyl heptadecanoate, retinyl stearate, retinyl isostearate, retinyl nonadecanoate, retinyl arachidonate, retinyl behenate, retinyl linoleate, oleate of retinyl, retinyl lactate, retinyl glycolate, retinyl hydroxycaprylate, retinyl hydroxylaurate, retinyl tartrate. e, the ester is selected from esters of C2, C3 and C1S (because they are the most commonly available) or linoleate ester because of its superior efficacy. A retinoid may be present in the compositions of the invention in an amount of 33 to 330,000 IU per gram of the composition, preferably 330 to 16,500 IU, more preferably 1.650 to 6,600 IU. The compositions preferably include kojic acid and yombin as depigmenting agents of the skin and / or phytic acid (as an anti-irritant) in an amount from 0.0001% to 50%, preferably from 0.001% to 25%. The compositions used in the method of the invention also comprise a cosmetically acceptable vehicle to act as a diluent, dispersant or carrier for the cosmetic benefit ingredient in the composition, so as to facilitate its distribution when the composition is applied to the skin. The vehicle can be aqueous, anhydrous or an emulsion. Preferably, the compositions are aqueous or an emulsion, especially a water-in-oil or oil-in-water emulsion. When the water is present, it will be in amounts which vary from 5 to 99%, preferably from 20 to 70%, and optimally between 35 and 60% by weight. In addition to water, relatively volatile solvents can also serve as carriers within the compositions of the present invention. Most preferred are C1-C3 monohydric alkanols. These include ethanol, methanol and isopropanol. The amount of monohydric alkanol can vary from 1 to 70%, preferably from 10 to 50%, and optimally between 15 and 40% by weight. The emollient materials can also serve as cosmetically acceptable carriers. These can be in the form of silicone oils and synthetic esters. The amounts of the emollients can vary in any range from 0.1 to 50%, preferably between 1 and 20% by weight. Silicone oils can be divided into volatile and non-volatile varieties. The term "volatile" as used herein, refers to those materials which have a measurable vapor pressure at room temperature. The volatile silicone oils are preferably chosen from cyclic or linear polydimethylsiloxanes containing 3 to 9, preferably 4 to 5, silicon atoms. Linear volatile silicone materials generally have viscosities less than about 5 centistokes at 25 ° C while cyclic materials typically have viscosity of less than about 10 centistokes. The non-volatile silicone oils useful as an emollient material include polyalkylsiloxanes, polyalkylarylsiloxanes and polyether siloxane copolymers. Essentially non-volatile polyalkylsiloxanes useful herein include, for example, polydimethylsiloxanes with viscosities from about 5 to about 25 million centistokes at 25 ° C. Among the preferred non-volatile emollients useful in the present compositions are polydimethylsiloxanes having viscosities from about 10 to about 400 centistokes at 25 ° C. Among these emollients are: (1) -Alkenyl esters or alkyl of fatty acids having 10 to 20 carbon atoms. Examples thereof include isoaraquidyl neopentanoate, isononyl isononanoate, oleyl myristate, oleyl stearate and oleyl oleate. (2) Ether esters such as fatty acid esters or ethoxylated fatty alcohols. (3) Polyhydric alcohol esters. Esters of mono and digraso acid of ethylene glycol, esters of mono and digraso acid of diethylene glycol, mono esters and fatty diacid of polyethylene glycol (200-6,000), esters of mono and di-fatty acid of propylene glycol, propylene glycol monooleate 2,000, polypropylene glycol monostearate 2,000, propylene glycol ethoxylated monostearate, glyceryl mono and digraso acid esters, polyglycerol polygrase esters, ethoxylated glyceryl monostearate, 1,3-butylene glycol monostearate, 1,3-butylene glycol distearate, polyoxyethylene polyol fatty acid esters, esters of fatty acid of sorbitan and esters of fatty acid of polyoexietilensorbitán, are the polyhydric alcohol esters satisfactory. (4) wax esters such as beeswax, spermaceti, myristyl myristate, stearyl stearate and arachidyl behenate. (5) Sterol esters, of which the cholesterol fatty acid esters are examples thereof.

Fatty acids having from 10 to 30 carbon atoms can also be included as cosmetically acceptable carriers for compositions of this invention. Illustrative of this category are pelargonic, lauric, myristic, palmitic, stearic, isostearic, hydroxystearic, oleic, linoleic, ricinoleic, arachidic, behenic and erucic acids. Humidifiers of the polyhydric alcohol type can also be used as cosmetically acceptable carriers in compositions of this invention. The humectant helps to increase the effectiveness of the emollient, reduces scale, stimulates the removal of build-up of scale and improves the feeling in the skin. Typical polyhydric alcohols include glycerol, polyalkylene glycols and more preferably alkylene polyols and their derivatives, which include propylene glycol, dipropylene glycol, polypropylene glycol, polyethylene glycol and derivatives thereof, sorbitol, hydroxypropyl sorbitol, hexylene glycol, 1,3-butylene glycol, 1,2,6-hexanetriol. , ethoxylated glycerol, propoxylated glycerol and mixtures thereof. For best results, the humectant is preferably propylene glycol or sodium hyaluronate. The amount of humectant can vary anywhere from 0.5 to 30%, preferably between 1 and 15% by weight of the composition. The thickeners can also be used as part of the cosmetically acceptable carrier of the compositions according to the present invention. Typical thickeners include crosslinked acrylates (for example Carbopol 982), hydrophobically modified acrylates (for example Carbopol 1382), cellulose derivatives and natural gums. Among the useful cellulose derivatives are sodium carboxymethylcellulose, hydroxypropylmethylcellulose, hydroxypropylcellulose, hydroxyethylcellulose, ethylcellulose and hydroxymethylcellulose. Natural gums suitable for the present invention include guar, xanthan, sclerotium, carrageenan, pectin and combinations of these gums. The amounts of the thickener can vary from 0.0001 to 5%, usually from 0.001 to 1%, and optimally from 0.01 to 0.5% by weight. Collectively, water, solvents, silicones, esters, fatty acids, humectants and / or thickeners will constitute a cosmetically acceptable carrier in amounts of 1 to 99.9%, preferably 80 to 99% by weight. An oil or an oily material may be present, together with an emulsifier, to provide a water-in-oil emulsion or an oil-in-water emulsion, depending mainly on the average hydrophilic-lipophilic balance (HLB) of the emulsifier used. The surfactants may also be present in the cosmetic compositions of the present invention. The total concentration of the surfactant will vary from 0.1 to 40%, preferably from 1 to 20%, optimally from 1 to 5% by weight of the composition. The surfactant can be selected from the group consisting of anionic, non-ionic active substances. , cationic and amphoteric. Particularly preferred nonionic surfactants are those with a C 10 -C 20 fatty alcohol or a hydrophobic acid fused with from 2 to 100 moles of ethylene oxide or propylene oxide per hydrophobic template; C2-C10 alkylphenols condensed with from 2 to 20 moles of alkylene oxide; esters of mono- and di-fatty acid of ethylene glycol; fatty acid monoglyceride; sorbitan, C8-C20 mono- and di-fatty acids; block copolymers (ethylene oxide / propylene oxide); and polyoxyethylene sorbitan as well as combinations thereof. The alkyl polyglycosides and fatty saccharide amides (for example methyl gluconoamides) are also suitable nonionic surfactants. Preferred anionic surfactants include soap, alkyl ether sulfate and sulfonates, alkyl sulfates and sulfonates, alkylbenzene sulphonates, alkyl and dialkyl sulfosuccinates, C8-C20 acyl isethionates, acyl glutamates, C8-C20 alkyl ether phosphates and combinations thereof. Sunscreens may be present in the cosmetic compositions of the present invention. Sunscreens include those materials commonly used to block ultraviolet light. Illustrative compounds are PABA derivatives, cinnamate and salicylate. For example, avobenzophenone (Parsol 1789), octyl methoxycinnamate and 2-hydroxy-4-methoxybenzophenone (also known as oxybenzone) can be used.Octyl methoxycinnamate and 2-hydroxy-4-methoxybenzophenone are commercially available under the trademarks commercials Parsol MCX and Benzophenone-3, respectively The exact amount of sunscreen used in the compositions may vary depending on the degree of protection desired against solar UV radiation Many cosmetic compositions, especially those containing water, should be protected against the growth of potentially harmful microorganisms Therefore, preservatives are necessary Suitable preservatives include alkyl esters of p-hydroxybenzoic acid, hydantoin derivatives, propionate salts and various quaternary ammonium compounds.The particularly preferred preservatives of this invention are methylparaben, propylparaben, phenoxyethanol and benzyl alcohol lic. Conservative usually be used in amounts ranging from about 0.1% to 2% by weight of the composition. Powders can be incorporated into the cosmetic composition of the invention. These powders include gypsum, talc, Fuller's earth, kaolin, starch, smectite clays, chemically modified magnesium aluminum silicate, organically modified montmorillonite clay, hydrous aluminum silicate, fumed silica, aluminum starch, octenyl succinate, and mixtures thereof.

The composition can be packaged in a suitable container to adjust to its viscosity and use proposed by the consumer. For example, a fluid lotion or cream can be packaged in a bottle or applicator with a rotating sphere, or a capsule, or a propellant-driven aerosol device or a container adapted with a pump suitable for operation with a finger. When the composition is a cream, it can simply be stored in a non-deformable bottle or in a container that can be pressed, such as a tube or a container with a lid. When used, the south pole of the magnet and the composition can be applied sequentially (either the composition or the magnet first) or concurrently to the same area of the skin. The magnet is applied to the skin for a period of time from minutes to hours. The composition, for example from 1 to 100 ml, is applied to exposed areas of the skin, from a suitable container or applicator and, if necessary, then sprayed and / or rubbed on the skin using the hand or fingers or a suitable device. The magnet and composition can be packaged together, as part of the same equipment, or sold or purchased separately.

MODALITY B: A DOUBLE COSMETIC PATCH In its second embodiment (B), the invention also includes a double cosmetic patch comprising: (i) a static magnet layer; (ii) a layer of cosmetic ingredient which carries a cosmetic composition comprising a cosmetic benefit ingredient; wherein the two layers are joined together and the south pole layer (i) is oriented towards the layer (ii). In this embodiment, the magnetic layer can be of any shape or size, but it is a thin film. The transdermal layer or a hydrogel layer transports the active cosmetic as described above for the A modality. The two layers are joined together with an adhesive system. The double patch can be shaped to suit the desired area of application, for example as a circle, as a strip for the nose, or as a rectangle. The double patch can be applied to the skin by means of a tape or an adhesive. The patch is preferably applied to the layer (ii) of the skin closest to the skin. The cosmetic benefit ingredient can be any of the ingredients which are beneficial to the skin, but is preferably selected from the cosmetic benefit ingredients described above for the A modality. The following specific examples further illustrate the invention, but the invention is not limit them.

Materials and methods The bar magnets used in these studies are obtained from MC Master Carr. New Brunswick, NJ. These magnets are permanent magnets, also called static magnets (as opposed to electromagnets that generate a magnetic field as well as an overlapping electric field) that have a north pole (N) and a south pole (S). In the experiments described here, where it is mentioned that the N or S pole is applied to the cells, it simply means that the N or S pole of the magnet is in close proximity to the cells, it is impossible to separate one pole from another in any magnet . The magnets used in these studies have a biomagnetic force of 700-750 gauss at the edges and 280-300 gauss in the middle part of the bar. Bioflex bioimanes used in these studies are obtained from BlOflex Inc., Oakland Park, FL. The Bioflex magnetic discs are permanent magnets which have concentric circles of alternating poles N and S placed in circles. This allows the cells to come into contact with a series of North-South poles. The Bioflex magnets used in these studies have a magnetic field of 40 gauss at the edge and 87 gauss in the middle part of the magnet. In all the experiments described here, in order to avoid the variability due to the difference in the magnetic field in different areas of the magnet (edge versus center), the cells are plated in the middle part of 60 wells of 96-well plates. wells which are placed in the middle part of the bar or Bioflex magnets. Similarly, pig skin biopsies are also placed in the middle part of the magnets. The cells used in these studies are normal human keratinocytes and pig skin organ cultures. Normal human keratinocytes isolated from the forearms of neonates by treatment with trypsin are grown in DME medium / 5% fetal bovine serum in the presence of mitomysin C treated with 3T3 mouse fibroblasts to establish dividing colonies. Keratinocytes are grown under these conditions until their third passage. For the experiments, the keratinocytes of the third passage are seeded in plates in a growth medium for serum-free keratinocytes (KGM).; obtained from Clonetics, San Diego, CA) which contains calcium 0.15 mM. Neonatal human fibroblasts are obtained from Clonetics and grown in minimal Dulbeco essential medium (DMEM) containing 10% fetal bovine serum. The experiments are carried out on cells between passages numbers 4-10. Skin of freshly cut 2-3 week old piglets is obtained from Buckshire Farms, PA. The skin of pigs is thoroughly washed first with soap and then with 10X antibiotic containing medium (penicillin, streptomycin). The pig skins are then dermatomized to 200 micrometers thick. 7mm perforation biopsies are performed from these washed pig skins. The biopsies are then washed again with 10X antibiotic medium and finally with normal DMEM (2 washes). 3 biopsies are incubated in each of the transverse well plates, with the epidermal side up with 1 ml of serum free DMEM medium fed from the bottom of the plates. The experiments begin 3 days after the biopsies have been balanced under these conditions.

Effect of magnetic force on the growth of keratinocytes Keratinocytes are seeded in the middle part of 60 wells of 96-well plates (3000 cells / well). 24 hours later, the plates are placed on top, in the middle part of a magnet (bar or Bioflex) and grown for an additional 4 days until the cells become 70-80% confluent. The medium is changed every 2 or 3 days. Control plates are placed on the top of a non-magnetic metal plate and the test plates are placed on the top of a bar magnet (with the north or south side facing up) or in the middle part of a Bioflex magnet. In some studies, where cell growth is measured as a function of magnetic force, the plates are placed on top of each other on top of a magnet so that the bottom plate is closest to the magnet (higher force) and the upper plate is further away from the magnet (minor magnetic force). Studies to determine the effects of the North and South magnets are carried out in different incubators to avoid the effluence of one magnet on another. After 4 days of growth, the plates are removed from the incubator, washed 2 times with phosphate buffered saline (PBS) and incubated with 100 μl of a solution of bisbenzimide H 33258 (obtained from Calbiochem - the concentrated solution is prepared to 1 mg / ml distilled water, stored in the dark at 4 ° C for no more than 1 week). After 15 minutes of incubation in the dark at room temperature, the fluorescence is measured in a Millipore Cytofluor 2000 (ex / e = 360/460 nm). The DNA content is calculated using standard bovine thymus DNA and expressed as μg / well or as% of the control wells which were not exposed to the magnets. In all studies using monolayer cultures, the mean +/- standard deviation of at least 24 different wells is used to calculate the statistical significance using Student's t test.

DNA synthesis of pig skin organ cultures 3 days after balancing the pig skin organ cultures prepared as described above, the medium is removed, 1 ml of fresh medium is added and the wells are treated with different concentrations of lactic acid when adding from a concentrate 100 times in water, directly in the middle. Simultaneously with the addition of lactic acid, the selected cultures are also exposed to the South or North pole of a bar magnet or to the Bioflex magnet. The magnets are placed on top of the culture vessel to expose the epidermis directly (organ culture is carried out with the epidermis side up). 3 days later, the medium is changed, lactic acid is added again and added to each well 10 μCi of 3H-imidine. The plates are incubated for an additional 24 hours. At the end of the incubation period, the biopsies are removed, washed in PBS for 1 hour with shaking and incubated overnight in 10 ml of 2M sodium bromide with shaking to divide the epidermis from the dermis. The biopsies are washed with PBS, and the epidermis is detached from the dermis carefully using a scalpel. The epidermis and dermis are digested with 1 ml of 0.5N NaOH overnight at 50 ° C, 200 μl is used for the radioactivity count. The rate of DNA synthesis is calculated as cpm / 7 mm of a perforation biopsy for dermis or epidermis and in some experiments is also expressed as% of controls. All organ culture experiments are carried out with at least 6 duplicates, and the mean +/- standard deviation is calculated for statistical analysis using Student's t test. 25 Example 1 Different thicknesses of magnets are compared. The effects of increasing the thickness (ie, increasing the magnetic force (Bioflex magnets) on the proliferation of keratinocytes in the cultures of monolayers of keratinocytes is investigated.

Thickness of the Iman Content of DNA% on the control Values P Control (without magnet) 820 +/- 22 0 0.75 cm 825 + 45 1.73 1 cm 855 +/- 20 5.12 > 0.1 1.5 cm 939 +/- 28 12.4 > 0.05 This example evaluates the effects of the different thickness of the Bioflex magnet on the proliferation of keratinocytes to determine which is the optimum force required for the effects. As seen in the table above, the proliferation effect is greater as the thickness of the magnet increases (and therefore the magnetic force). Magnets with a thickness of 1.5 cm or greater significantly increase the proliferation of keratinocytes in monolayer cultures.

Example 2 The differences between Bioflex magnets (weakest forces, 70-100 gauss) and bar magnets with higher magnetic forces (300-400 gauss) were evaluated. The bar magnet is used with the South Pole to the cells in this study. Magnets increase the proliferation of keratinocytes in 3 separate studies, however, bar magnets show higher increases compared to Bioflex magnets in 2 of the 3 studies.

Example 3 Example 3 evaluates the synergy between lactic acid and the magnetic field to induce the proliferation of keratinocytes. In this study, using in vitro keratinocyte cultures, 2 mM lactic acid increases proliferation by 5.4%. This increase is not statistically significant. The Bioflex magnet alone also stimulates proliferation by 5.5%, which is not statistically significant either, under the conditions used for the experiment. However, the lactic acid in the presence of the Bioflex magnet stimulates the proliferation of keratinocytes by 15%, which is statistically significant (p> 0.001). Therefore, this experiment reveals the synergy between lactic acid and the magnetic force in the stimulation of keratinocyte proliferation. Synergy between lactic acid and the magnetic field in the proliferation of keratinocytes measured on the basis of the DNA content: Deviation 5.3 6.3 15 standard value p 0.35 0.36 0.001 Example 4 Example 4 compares the geometry of the magnet (North versus South, bar magnet versus circular magnet BlOflex) on the proliferation of epidermal cells from pig skin organ culture. Pig skin culture is carried out as described in the methods. The Bioflex magnet does not have a significant effect on epidermal proliferation in this experiment. The bar magnet (both North and South) show greater stimulation of proliferation. The effect of the North pole of the bar magnet is minimal (24% over the control) and is not statistically significant. However, the South pole of the bar magnet significantly stimulates the proliferation (87% over control) of pig epidermal cells. This study suggests that the bar magnet is better than the Bioflex magnet (due to the greater magnetic field) and that the South pole of the bar magnet is significantly better than the North pole.

Comparison of "the different magnet geometry on epidermal proliferation in organ culture Example 5 Example 5 evaluates the dose response to lactic acid (LA) and the synergy between lactic acid and the South pole of the bar magnet in the proliferation of pig epidermal cells. The effect of lactic acid is significant only at 2.0 mM, lower levels, of 0.02 and 0.2 mM, do not have a significant effect on epidermal proliferation in this experiment. As noted above, the South pole of the bar magnet has a significant influence on proliferation. As would be expected, the South pole magnet in the presence of different concentrations of lactic acid always presents a significantly higher proliferation compared to the controls. When compared to the South Pole magnet alone, only the highest lactic acid is significantly different (similar to the finding where only the highest lactic acid is significant). Synergies are observed with LA and the South pole (LA 2 mM is only 43% over the control, the South pole is only 87% over the control, LA + South pole is 165% over the control, which is greater than a response additive 43 + 87 = 130).

Example 6 Example 6 illustrates the comparison between the South and North pole magnet for its synergy with lactic acid in epidermal proliferation in pig skin. The skin exposed to the South pole shows a higher rate of proliferation than the skins exposed to the North pole. 0.2 mM lactic acid does not have a significant effect on epidermal proliferation (data similar to those in Example 5). However, when combined with the North or South pole magnet, 0.2 mM lactic acid shows a significantly higher proliferation rate compared to the control. The South + LA group shows the maximum proliferative response (146% over the control) and is synergistic when compared to LA alone or South pole only as a control. The North + LA group shows 112% on the control proliferative response, but this is not synergistic when compared to the control of the North pole alone. Therefore, this experiment confirms the previous finding that lactic acid plus the South pole magnet show a synergistic growth response of epidermal cells in pig skin organ cultures.

Example 7 In Example 7, dermal proliferation is measured as a function of lactic acid and treatment with the magnetic pole. As with the epidermis, the south pole shows a greater increase than the north pole. Lactic acid only has a significant stimulation of dermal proliferation. However, in contrast to the findings of the epidermis, the combinations of magnet with lactic acid do not show synergy in the dermis.

Example 8 Patches are constructed to provide a south-facing magnetic layer bonded to a substrate layer containing active substance for application to the skin so that the active substance migrates from its layer to the skin within the oriented magnetic field. There are four constructions as detailed below: 8A. Nonwoven patch A. Magnetic sheet (with the south side down) B. Adhesive layer C. Nonwoven material impregnated with adhesive and active substance (Optional) D. Adhesive layer.

Example: hydroxy acid is combined with excipients and a water soluble adhesive and impregnated into the nonwoven material. An example of an adhesive / active composition is: 80% of an anionic polymer such as Gantrez S-97 2% aminomethylpropanol 6% lactic acid 0.2% preservative q.s. Water The layer B is joined to the layer A with the layer C and is an adhesive selected from table 1 which is compatible with the layers A and C. The layer C is a nonwoven material impregnated with an active substance and a water soluble adhesive to bind to the skin. The adhesive is selected from table 2. In an alternative form of the product, the adhesive in a C layer is used to adhere layer C to layer A. Another alternative form is when layer A, a pressure sensitive adhesive ( PSA), is applied to the non-woven material. The PSA is applied as a continuous layer or as a continuous layer which is semipermeable to water and the active substance. 8B. Hydrogel patch A. Magnetic sheet (with the south side down) B. Adhesive layer C. Nonwoven material impregnated with hydrogel and a hydroxy acid (Optional) D. Adhesive layer.

Example: a non-woven polyester material with a gel impregnated in a 1:10 ratio. An example of a gel composition is: 55% water 28% NA salt of polyacrylate derivative 15% glycerin 1% xanthan gum 1% NaCl The layer B joins the layer A to the layer C and is an adhesive that is selected from table 1, which is compatible with the layers A and C. The adhesion of the layer C to the skin is obtained by the surface tension of the gel. An alternative way is when layer D, a pressure sensitive adhesive (PSA), is applied to the nonwoven material. The PSA is applied as a non-continuous layer or as a continuous layer which is semipermeable to water and the active substance. 8C. Pressure sensitive adhesive patch (PSA).

A. Simple example: adhesive with glycolic acid A. Magnetic sheet (with the south side down) (Optional) B. Adhesive layer (Optional) F. Plastic film C. Adhesive layer with active substance mixed in the example: 5% glycolic acid.

Example of liquid deposit: % glycerol 4% succinic acid 2% glycolic acid 1% carbopol 0.2% preservative q.s. Water B. Controlled release A. Magnetic sheet (with the south side down) B. Adhesive layer F. Plastic film Cl. Liquid tank with 2% lactic acid C2. Semipermeable membrane (speed control) D. Adhesive layer.

Example of liquid deposit: 5% "glycerol 4% succinic acid 2% glycolic acid 1% carbopol 0.2% preservative q.s. water.

In the simple patch, the adhesive in layer C is selected from table 1, and may be suitable for bonding to layer A. If not, layers B and F may be added. Layer B is an adhesive selected from table 1 which is compatible with layers A and C. Layer F is a plastic film constructed of polyurethane, polyester, polyethylene or polyvinyl chloride. The film thickness can vary from 1.0 ml to 254 mm (10.0 thousandths of an inch). The film can also be a foam, with a thickness between 508-5080 mm (20-200 thousandths of an inch). The controlled release patch uses layers B and F to separate and contain the reservoir containing liquid plus the active substance or substances from the magnetic magnet. The adhesive layer D may be a non-continuous layer applied to the semipermeable membrane or as a continuous layer which does not significantly affect the flow of water and active substances. 8D. Silicone sheet patch The layer B joins the layer A with the layer C and is an adhesive that is selected from table 1 which is compatible with the layers A and C. The silicone sheet is of the type suitable for the treatment of scratches, wounds, Stretch marks and / or other skin conditions. The fluid or gel of silicone in the bag is similar to the type useful for treating skin conditions. Table 1 Classes of adhesives Table 2 Classes of water soluble adhesives Example 9 Phytic acid (0.5%) is examined for its effect on cytokine release by the live skin equivalents of MelanoDerm in the presence and absence of south pole magnetism. After 24 hours of incubation, during which one set of tissues is exposed to south pole magnetism and a duplicate equivalent series is not, it is assayed in culture medium for determination of IL1-a and PGE-2. In the absence of magnetism there is a significant increase in the medium concentration of both IL1-a and PGE-2. These increases are suppressed by the presence of south pole magnetism. materials Live Skin Equivalent: MelanoDerm "11 MEL-300, a three-dimensional epidermal model containing functioning melanocytes produced by MatTek Corporation, Ashland, MA, which is used as the tissue for this experiment.

Phytic acid Phytic acid is tested at a concentration of 0.5% in water. It is demonstrated that this concentration is non-toxic for the melanoDerm tissue (without effect on the viability of the tissue determined by MTT) in a preliminary experiment.

Magnet: 15.2 cm x 10.2 cm x 2.5 cm (6 inch x 4 inch x 1 inch) rectangular permanent oriented Ferrimag ceramic magnet (3.4 megagauss oersteds) from Edmund Scientific Co., provides a field strength of approximately 650 +/- 200 gauss at an incubation plate level above the surface of the magnet.

Methods In Vitro Incubation: 50 microliters of phytic acid solution were applied (0.5%) to the surface of MEL-300 corneo tissue extract. 8 untreated MEL-300 tissues served as controls. Half of the tissues (ie, 2 of phytic acid and 4 untreated controls) were placed on the surface of the 15.2 cm x 10.2 cm (6 inches x 4 inches) south pole of the Ferrimag magnet during 24 hours of incubation (37 degrees Celsius; 5% of C02). After incubation for 24 h, tissues are removed from the incubation medium and assayed for MTT to confirm tissue viability. The incubation medium is assayed to determine the content of cytokines IL1-a and PGE-2. The tissue samples are refrigerated and a second cytokine determination is made 7 days later. Cytokine assays are performed using commercially available 96 well Elisa kits.

Results Results of Elisa for IL1-a and PGE-2 are summarized in Table I and are detailed in Table II.

Table I Effect of magnetism on stimulation with phytic acid of cytokine release These results demonstrate that magnetism suppresses the stimulation by phytic acid of IL1-a and PGE-2 of epidermal skin tissue. Both ILl-a and PGE-2 are inflammatory mediators, the suppression of them by magnetism is indicative of an anti-inflammatory effect of magnetism. Since the magnet does not have a significant effect on the release of cytokine in the absence of active substance, the results indicate an anti-inflammatory effect of magnetism on the action of active substances such as phytic acid which stimulates the cutaneous production of inflammatory mediators.

Table 2. Effect of phytic acid on tissue release IL1-a and PGE-2 It can be seen from the results in tables I and II that the magnetism of the south pole inhibits the proinflammatory action of phytic acid on the epidermal skin tissue.

Example 10 Kojic acid, vitamin C and yombin are examined to determine their ability to reduce pigmentation in the living cutaneous equivalent of malenoderm in the presence and absence of South Pole magnetism. After 14 days of incubation, during which a series of tissues is exposed to the South Pole magnetism and a duplicate equivalent series is not detected, the melanin color formed in each tissue is estimated using primary color image analysis. The results show a reduction in pigmentation for each of the activities in the absence of magnetism and an increased reduction in pigmentation, in the presence of South Pole magnetism. materials Equivalent of living skin: For this experiment the tissue used is MelanoDermm MEL-300, a three-dimensional epidermal model that contains functioning melanocytes produced by MatTek Corporation, Ashland, MA, each MEL-300 team has 24 pieces of fabric.

Active depigmentation substances Three active depigmentation substances were added to the Melanoderm incubation medium at the following final concentrations: (a) Acid-kojic - 500 micromolar (b) Yombin - 25 micromolar (O Ascorbic acid - 285 micromolar Magnet: A Ferrimag ceramic magnet oriented permanently rectangular of 15.2 cm x 10.2 cm x 2.5 cm is used (6 inches x 4 inches x 1 inch) (3.4 megagauss oersteds) from Edmund Scientific Co. , which provides a field strength of approximately 650 +/- 200 gauss at the level of incubation plate above the surface of the magnet.

Methods In vitro incubation Two MEL-300 devices are prepared, each with 24 pieces of tissue, with identical application of active substances. Each active substance, vitamin C, kojic acid and yombin is applied to the incubation medium of 5 tissues at the concentrations specified above. 4 tissues are not treated (the remaining 5 tissues are treated with a melanin-stimulating active substance not reported here because it has no significant effect on pigmentation). A MEL-300 equipment prepared as above is placed on a 15.2 cm x 10.2 cm (6 inch x 4 inch) South pole surface of the Ferrimag magnet for incubation, and the second identical MEL-300 equipment is incubated on the side opposite (top left versus bottom right) of the same incubator and is additionally protected from the magnet. The fabrics are incubated for 14 days in a maintenance medium at 37 ° C and a constant atmosphere (C02 5%). The maintenance medium and active substances are re-supplied four times a week. After 7 days, 2 tissues were removed from each group of active and control substances, leaving 3 tissues in each group (2 in the untreated group) for evaluation at 14 days.

Determination of melanin pigment production: On day 14 of the end of the experiment, the tissue pieces of each Mel-300 incubation plate are photographed from the top. Each tissue appears as a small disc that varies in intensity of brown. Photographs of two Mel-300 incubation plates (one incubated on the magnet and one without magnetism) are scanned in a Pentium II computer and the primary color content in the color is measured, of each disk, using a macro program of color analysis with a software package of image quantification. Optimal.- The content of blue color is used as a melanin pigment index (preliminary experiments confirm that the principle of primary color of Melanin is blue using this image analysis system - this is established by comparing brown freckles and age spots with surrounding non-pigmented skin in color photographs of Caucasian human skin). The intensity scale of blue color is 0-255, where 0 is saturation, with blue color and 255 is total absence of blue color. The actual blue scale read on each tissue sample is converted to a blue value in% by a calculation of the proportion in the entire range from 0 to 255. The key findings are presented in the table below.

Effect of magnetism on the activity of depigmenting active substances In the absence of magnetism, the 3 depigmenting active substances show less pigmentation than the untreated control tissue. The tissues incubated on the south pole side of the magnet show a greater pigmentation reduction in relation to the untreated control than that observed without the magnet. The difference between the active substances in the presence and absence of magnetism is statistically significant (p <0.05). The pigmentation observed in the control tissues is not significantly different for the group without magnet (78%) and with magnet (79%), which shows that in the absence of active substances, the magnetism of South Pole does not have a depigmenting effect . Therefore, the results show that South Pole magnetism improves the skin depigmentation action of kojic acid, yombin and vitamin C.

Example 11 This example measures the effect of the various active substances in combination with magnets on the production of lipids by sebocytes in vitro. Secondary cultures of human sebocytes obtained from a male adult are grown in 24 wells of 48 well tissue culture plates (Costar Corp, Cambridge Mass) until confluence. The growth medium of sebocytes consists of Clonetics keratonic acid base medium (KBM) supplemented with 14 g / μml of bovine hypophysis extract, 0.4 μg / ml of hydrocortisone, 5 / xg / ml of insulin, 10 ng / ml of factor epidermal growth, cholera toxin 1.2 x 1010 M, 100 units / ml penicillin and 100 μg / ml streptomycin. All cultures were incubated at 37 ° C in the presence of C02 7.5%. The medium is changed three times a week. On the first day of experimentation, the growth medium is removed and the sebocytes are washed three times with sterile Dulbecco modified Eagle's medium (DMEM, free of phenol red). Fresh DMEM is added to each sample (triplicates) with 5 microliters of test agent solubilized in ethanol (312.5 μM iso-tridecylsalicylate or 10 μM retinyl linoleate, 6 duplicates in each sample). The controls consist of the addition of ethanol alone. Each magnet is interposed between two plates for 24 hours. For two of the sebocyte plates, the configuration is with the North pole pointing upwards, for two other plates, the configuration is inverted (the magnet with the South pole facing up) and a plate serves as a control (without the presence of magnets) . After 24 hours, the addition of 14C-acetate buffer (5 mM final concentration, 56 mCi / mmoles of specific activity) is performed. The sebocytes are then returned to the incubator for four hours, after which each culture is rinsed three times with phosphate-buffered saline to remove the unbound labeled material. The radioactive labeled material remaining in the sebocytes is collected and counted using a Beckman scintillation counter. The statistical analysis is done using the JMP software package.

Comparisons for each pair using Student's t test Alpha = 0.05 Positive values show pairs of means that are significantly different Abbreviations: Cont: Control plate without magnets TDS: iso-tridecilsalicilato RL: retinyl linoleate NT: North magnet facing upwards, tissue culture plate above the magnet NB: North magnet facing upward, tissue culture plate under the magnet ST: South magnet facing upwards, tissue culture plate above the magnet SB: South magnet facing upwards, tissue culture plate under the magnet.

Claims (4)

1. A double cosmetic patch, comprising: (i) a static magnet layer; (ii) a non-woven, transdermal patch of hydrogel or silicone laminate carrying a beneficial cosmetic ingredient; wherein the two layers are adhesively bonded together.

2. A cosmetic method for treating the skin, the method comprises: (a) applying to the skin the South pole of a static magnet; Y (b) applying to the skin a composition for the cosmetic care of the skin comprising a cosmetic beneficial ingredient in a cosmetically acceptable vehicle.

3. The method according to claim 2, characterized in that the thickness of the magnet is at least 1 cm.

4. The method according to claim 2, characterized in that the cosmetic benefit ingredient is selected from the group consisting of hydroxy acid, retinoids, vitamin C, phytic acid, yombine and kojic acid.