[go: up one dir, main page]

MXPA96004278A - Expansion diferencial de celulas de baston fetalesen la circulacion materna para usarse en analisisgenetico prenatal. - Google Patents

Expansion diferencial de celulas de baston fetalesen la circulacion materna para usarse en analisisgenetico prenatal.

Info

Publication number
MXPA96004278A
MXPA96004278A MXPA96004278A MX9604278A MXPA96004278A MX PA96004278 A MXPA96004278 A MX PA96004278A MX PA96004278 A MXPA96004278 A MX PA96004278A MX 9604278 A MX9604278 A MX 9604278A MX PA96004278 A MXPA96004278 A MX PA96004278A
Authority
MX
Mexico
Prior art keywords
fetal
cells
disease
sought
stem cells
Prior art date
Application number
MXPA96004278A
Other languages
English (en)
Inventor
P Alter Blanche
Original Assignee
Univ Texas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Texas filed Critical Univ Texas
Publication of MXPA96004278A publication Critical patent/MXPA96004278A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0641—Erythrocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6863—Cytokines, i.e. immune system proteins modifying a biological response such as cell growth proliferation or differentiation, e.g. TNF, CNF, GM-CSF, lymphotoxin, MIF or their receptors
    • G01N33/6869—Interleukin
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/125—Stem cell factor [SCF], c-kit ligand [KL]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/14—Erythropoietin [EPO]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Urology & Nephrology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Developmental Biology & Embryology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Los metodos de la presente invencion usan sangre de mujeres embarazadas y amplificacion de celulas fetales para facilitar el diagnostico prenatal sin invadir el espacio fetal. Este metodo es menos invasor y mas simple que la amniocentesis o el muestreo de vellosidad corionica porque solamente involucra extraer sangre de la madre para obtener una muestra de celulas fetales. Si se esta buscando una enfermedad especifica con mutaciones moleculares conocidas, las celulas de la muestra son una fuente de DNA enriquecido para el material fetal. Si la enfermedad involucra a los globulos rojos sanguineos, este metodo proporciona globulos rojos sanguineos fetales para su analisis. Si la enfermedad involucra globulos blancos sanguineos, se pueden usar distintas citocinas para producir selectivamente leucocitos fetales. Si se esta buscando un patron de cromosomas anormales sin un riesgo especifico de diagnostico (por ejemplo, por edad avanzada de la madre), entonces el metodo de cultivo proporciona celulas en division de origen fetal. La figura mas representativa de la invencion es la numero 6.
MXPA96004278A 1994-03-25 1995-03-24 Expansion diferencial de celulas de baston fetalesen la circulacion materna para usarse en analisisgenetico prenatal. MXPA96004278A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/218,290 US5580724A (en) 1994-03-25 1994-03-25 Differential expansion of fetal stem cells in maternal circulation for use in prenatal genetic analysis
PCT/US1995/003659 WO1995026507A1 (en) 1994-03-25 1995-03-24 Differential expansion of fetal stem cells in the maternal circulation for use in prenatal genetic analysis

Publications (1)

Publication Number Publication Date
MXPA96004278A true MXPA96004278A (es) 2002-04-19

Family

ID=22814517

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA96004278A MXPA96004278A (es) 1994-03-25 1995-03-24 Expansion diferencial de celulas de baston fetalesen la circulacion materna para usarse en analisisgenetico prenatal.

Country Status (9)

Country Link
US (1) US5580724A (es)
EP (1) EP0752104B1 (es)
JP (1) JP2996513B2 (es)
AU (1) AU684935B2 (es)
CA (1) CA2186366C (es)
DE (1) DE69501326T2 (es)
ES (1) ES2111401T3 (es)
MX (1) MXPA96004278A (es)
WO (1) WO1995026507A1 (es)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5731156A (en) * 1996-10-21 1998-03-24 Applied Imaging, Inc. Use of anti-embryonic hemoglobin antibodies to identify fetal cells
US6617158B1 (en) * 1999-08-19 2003-09-09 New England Medical Center Method for stimulating the production of fetal hemoglobin producing erythroid cells
US7666615B2 (en) * 2001-01-29 2010-02-23 Hemogenix, Inc. High-throughput assay of hematopoietic stem and progenitor cell proliferation
DE60235967D1 (de) * 2001-01-29 2010-05-27 Ivan N Rich ATP-basierter Assay zur Bestimmung der Proliferation hämatopoetischer Stamm- und Vorläuferzellen
US7989178B2 (en) * 2001-01-29 2011-08-02 Hemogenix, Inc. Colony assay miniaturization with enumeration output
US7354730B2 (en) * 2002-01-29 2008-04-08 Hemogenix, Inc. High-throughput assay of hematopoietic stem and progenitor cell proliferation
FR2824144B1 (fr) * 2001-04-30 2004-09-17 Metagenex S A R L Methode de diagnostic prenatal sur cellule foetale isolee du sang maternel
US8021876B2 (en) * 2001-11-15 2011-09-20 Children's Medical Center Corporation Methods of isolation, expansion and differentiation of fetal stem cells from chorionic villus, amniotic fluid, and placenta and therapeutic uses thereof
ATE324903T1 (de) * 2003-02-18 2006-06-15 Clinique La Prairie Res Sa Zusammensetzungen enthaltend fötales hämoglobin und bakterielles endotoxin und fakultativ zusätzliche fötale leberkomponenten
AU2004288722A1 (en) 2003-11-04 2005-05-26 U.S. Department Of Veterans Affairs Stem cell culture medium and method of using said medium and the cells
JP2008506637A (ja) * 2004-06-14 2008-03-06 ザ・ボード・オブ・トラスティーズ・オブ・ザ・ユニバーシティ・オブ・イリノイ 胎児起源の細胞に対する抗体
KR101900809B1 (ko) 2005-12-29 2018-09-20 안트로제네시스 코포레이션 태반 줄기 세포 집단
AU2007311126A1 (en) * 2006-10-16 2008-04-24 Celula Inc. Methods and compositions for differential expansion of fetal cells in maternal blood and their use
AR093183A1 (es) 2010-12-31 2015-05-27 Anthrogenesis Corp Aumento de la potencia de celulas madre de placenta usando moleculas de arn moduladoras
AU2012262273B2 (en) 2011-06-01 2017-09-14 Celularity Inc. Treatment of pain using placental stem cells

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2081203A1 (en) * 1990-04-23 1991-10-24 Ronald J. Berenson Method for enriching fetal cells from maternal blood
WO1993008268A1 (en) * 1991-10-23 1993-04-29 Cellpro, Incorporated Methods for selectively expanding stem cells
JPH07500499A (ja) * 1991-10-23 1995-01-19 セルプロ　インコーポレイテッド 母親の血液由来の胎児始原細胞を濃厚化する方法
WO1993018136A1 (en) * 1992-03-05 1993-09-16 Cytomed, Inc. Process for supporting hematopoietic progenitor cells
CA2154787A1 (en) * 1993-01-27 1994-08-04 Peter W. Runstadler Selective cell proliferation

Also Published As

Publication number Publication date
AU2229895A (en) 1995-10-17
DE69501326D1 (de) 1998-02-05
ES2111401T3 (es) 1998-03-01
CA2186366A1 (en) 1995-10-05
EP0752104B1 (en) 1997-12-29
US5580724A (en) 1996-12-03
WO1995026507A1 (en) 1995-10-05
EP0752104A1 (en) 1997-01-08
DE69501326T2 (de) 1998-04-16
JPH09509328A (ja) 1997-09-22
AU684935B2 (en) 1998-01-08
CA2186366C (en) 2008-07-08
JP2996513B2 (ja) 2000-01-11

Similar Documents

Publication Publication Date Title
Bianchi Circulating fetal DNA: its origin and diagnostic potential—a review
Bianchi Fetal cells in the mother: from genetic diagnosis to diseases associated with fetal cell microchimerism
MXPA96004278A (es) Expansion diferencial de celulas de baston fetalesen la circulacion materna para usarse en analisisgenetico prenatal.
US6664056B2 (en) Non-invasive prenatal monitoring
EP0791659A3 (en) Antigen specific separation of nucleated fetal cells in non-invasive detection of fetal DNA
US20080108071A1 (en) Methods and Systems to Determine Fetal Sex and Detect Fetal Abnormalities
IL129382A0 (en) Use of anti-embryonic hemoglobin antibodies to identify fetal cells
EP3444357B1 (en) Noninvasive detection method for screening healthily-growing blastulas
Adinolfi et al. Detection of fetal cells in transcervical samples and prenatal diagnosis of chromosomal abnormalities
Wang et al. Real-time PCR evaluation of cell-free DNA subjected to various storage and shipping conditions
Bianchi et al. Biological implications of bi-directional fetomaternal cell traffic: a summary of a National Institute of Child Health and Human Development-sponsored conference
Babochkina et al. Numerous erythroblasts in maternal blood are impervious to fluorescent in situ hybridization analysis, a feature related to a dense compact nucleus with apoptotic character
Tutschek et al. Isolation of fetal cells from transcervical samples by micromanipulation: molecular confirmation of their fetal origin and diagnosis of fetal aneuploidy
Giambona et al. Identification of embryo–fetal cells in celomic fluid using morphological and short‐tandem repeats analysis
Giambona et al. Fetal aneuploidy diagnosed at celocentesis for early prenatal diagnosis of congenital hemoglobinopathies
Sekizawa et al. Development of noninvasive fetal DNA diagnosis from nucleated erythrocytes circulating in maternal blood
Rapola et al. Palcental pathology and prenatal diagnosis of infantile type of neuronal ceroid‐lipofuscinosis
Spencer et al. First trimester prenatal diagnosis of trisomy 21 in discordant twins using fetal nuchal translucency thickness and maternal serum free β‐hCG and PAPP‐A
Hamada et al. Fetal nucleated cells in maternal peripheral blood after delivery in cases of fetomaternal hemorrhage
Makrydimas et al. Prenatal paternity testing using DNA extracted from coelomic cells
Delatycki et al. Trisomy 13 mosaicism at prenatal diagnosis: dilemmas in interpretation
Nisani et al. The dilemma of chromosomal mosaicism in chorionic villus sampling—‘direct’versus long‐term cultures
Chiu et al. Non-invasive prenatal diagnosis: on the horizon?
Hyodo et al. No correlation between the number of fetal nucleated cells and the amount of cell‐free fetal DNA in maternal circulation either before or after delivery
Poenaru et al. Prenatal diagnosis of mucolipidosis type II on first‐trimester amniotic fluid

Legal Events

Date Code Title Description
FG Grant or registration
MM Annulment or lapse due to non-payment of fees