MXNL06000069A - System for expressing and transporting recombinant proteins to the escherichia coli periplasm using the tat (twin-arginine translocation) secretion pathway. - Google Patents
System for expressing and transporting recombinant proteins to the escherichia coli periplasm using the tat (twin-arginine translocation) secretion pathway.Info
- Publication number
- MXNL06000069A MXNL06000069A MXNL06000069A MXNL06000069A MX NL06000069 A MXNL06000069 A MX NL06000069A MX NL06000069 A MXNL06000069 A MX NL06000069A MX NL06000069 A MXNL06000069 A MX NL06000069A
- Authority
- MX
- Mexico
- Prior art keywords
- tat
- escherichia coli
- transport
- coli periplasm
- pathway
- Prior art date
Links
- 241000588724 Escherichia coli Species 0.000 title abstract 3
- 210000001322 periplasm Anatomy 0.000 title abstract 3
- 230000009962 secretion pathway Effects 0.000 title abstract 2
- 239000004475 Arginine Substances 0.000 title 1
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 title 1
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 title 1
- 230000005945 translocation Effects 0.000 title 1
- 108090000623 proteins and genes Proteins 0.000 abstract 6
- 235000018102 proteins Nutrition 0.000 abstract 4
- 102000004169 proteins and genes Human genes 0.000 abstract 4
- 108020004705 Codon Proteins 0.000 abstract 3
- 108010076504 Protein Sorting Signals Proteins 0.000 abstract 3
- 230000032258 transport Effects 0.000 abstract 3
- 239000013604 expression vector Substances 0.000 abstract 2
- 230000037361 pathway Effects 0.000 abstract 2
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 abstract 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 abstract 1
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 abstract 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 abstract 1
- 108010073038 Penicillin Amidase Proteins 0.000 abstract 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 abstract 1
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 abstract 1
- 235000004279 alanine Nutrition 0.000 abstract 1
- 238000010367 cloning Methods 0.000 abstract 1
- 230000004927 fusion Effects 0.000 abstract 1
- 230000028327 secretion Effects 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention describes an expression vector which contains a signal peptide using the Tat secretion pathway as an alternative of the Sec secretion system for the transport of cytoquinine and further proteins of biotechnological interest to Escherichia coli periplasm. The novel system consists in using the signal peptide of penicillin acylase (SPpac) which is mutated and fussed to the synthetic human interferon-y gene (hINF-y) for the transport of the protein to the Escherichia coli periplasm using the Tat pathway. The mutated SPpac includes the NdeI site at the 5¿ end, which is contained in the expression vector pEMR, at the 3¿ end the tryptophan codon being exchanged by the serine codon, thus generating the restriction site Hind III. The Leucine and Alanine codons are modified so as to generate the NheI site. Thus, two sites for restricting the cloning and fusion within a gene phase of homologous or heterologous proteins are generated. Nowadays there is no commercial vector available for the expression and transport of proteins, which include signal peptides using the Tat pathway.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MXNL06000069 MXNL06000069A (en) | 2006-09-15 | 2006-09-15 | System for expressing and transporting recombinant proteins to the escherichia coli periplasm using the tat (twin-arginine translocation) secretion pathway. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MXNL06000069 MXNL06000069A (en) | 2006-09-15 | 2006-09-15 | System for expressing and transporting recombinant proteins to the escherichia coli periplasm using the tat (twin-arginine translocation) secretion pathway. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MXNL06000069A true MXNL06000069A (en) | 2008-10-09 |
Family
ID=40941204
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MXNL06000069 MXNL06000069A (en) | 2006-09-15 | 2006-09-15 | System for expressing and transporting recombinant proteins to the escherichia coli periplasm using the tat (twin-arginine translocation) secretion pathway. |
Country Status (1)
| Country | Link |
|---|---|
| MX (1) | MXNL06000069A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016071707A1 (en) * | 2014-11-07 | 2016-05-12 | University Of Kent | Biopharmaceutical production method |
| US12275685B2 (en) | 2018-12-03 | 2025-04-15 | Board Of Regents, The University Of Texas System | Oligo-benzamide analogs and their use in cancer treatment |
-
2006
- 2006-09-15 MX MXNL06000069 patent/MXNL06000069A/en active IP Right Grant
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2016071707A1 (en) * | 2014-11-07 | 2016-05-12 | University Of Kent | Biopharmaceutical production method |
| US10457948B2 (en) | 2014-11-07 | 2019-10-29 | University Of Kent | Biopharmaceutical production method |
| US12275685B2 (en) | 2018-12-03 | 2025-04-15 | Board Of Regents, The University Of Texas System | Oligo-benzamide analogs and their use in cancer treatment |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |