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MX9601719A - Metodo para incrementar de trehalosa de los organismos por medio de su transformacion con el adnc de la trehalosa-6-fosfato sintasa/fosfatasa de selaginella lepidophylla. - Google Patents

Metodo para incrementar de trehalosa de los organismos por medio de su transformacion con el adnc de la trehalosa-6-fosfato sintasa/fosfatasa de selaginella lepidophylla.

Info

Publication number
MX9601719A
MX9601719A MX9601719A MX9601719A MX9601719A MX 9601719 A MX9601719 A MX 9601719A MX 9601719 A MX9601719 A MX 9601719A MX 9601719 A MX9601719 A MX 9601719A MX 9601719 A MX9601719 A MX 9601719A
Authority
MX
Mexico
Prior art keywords
trehalose
cells
complementary dna
sintase
phosphate
Prior art date
Application number
MX9601719A
Other languages
English (en)
Other versions
MX205414B (es
MXPA96001719A (es
Inventor
Rodolfo Zentella Gomez
Gabriel Iturriaga De La Fuente
Original Assignee
Univ Mexico Nacional Autonoma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Mexico Nacional Autonoma filed Critical Univ Mexico Nacional Autonoma
Priority to MX9601719A priority Critical patent/MX205414B/es
Priority to JP9539760A priority patent/JP2000509602A/ja
Priority to BR9710436A priority patent/BR9710436A/pt
Priority to PCT/MX1997/000012 priority patent/WO1997042327A2/es
Priority to EP97923309A priority patent/EP0933428A2/en
Priority to AU29135/97A priority patent/AU727509B2/en
Publication of MX9601719A publication Critical patent/MX9601719A/es
Publication of MXPA96001719A publication Critical patent/MXPA96001719A/es
Publication of MX205414B publication Critical patent/MX205414B/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • General Preparation And Processing Of Foods (AREA)
  • Seasonings (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)

Abstract

Se clono y determino la secuencia nucleotídica de una molécula completa de ADN complementario que codifica para la ezima bifuncional trehalosa-6-fosfato sintasa/fosfatasa de plantas. La molécula de ADN complementario codifica par auna enzima bifuncional que sintetiza trehalosa. El ADN complementario se puede subclonar en vectores apropiados para su expresion en células huésped. Las células transformadas producen trehalosa, en contraste con las células no transformadas, lo cual aumentará la tolerancia al estrés por calor, frío, salinidad y sequía. Esta invencion se podrá usar para mejorar la termotolerancia y osmotolerancia de las plantas cultivadas en zonas áridas o semiáridas y también contribuirá a disminuir el uso de agua de riego. Por otro lado, la trehalosa producida en plantas transgénicas podría incrementar la vida de anaquel de los productos agrícolas, preservándolos por períodos largos en estado deshidratado sin que estos pierdan sus propiedades de olor, sabor y textura, una vez que sean rehidratados. Por ultimo, la sobreproduccion de trehalosa con bacterias, levaduras, hongos, células animales o plantas transgénicas constituirá una fuente barata de este producto para ser usado como aditivo para preservar diversos productos biologicos o alimentos procesados.
MX9601719A 1996-05-08 1996-05-08 Metodo para incrementar de trehalosa de los organismos por medio de su transformacion con el adnc de la trehalosa-6-fosfato sintasa/fosfatasa de selaginella lepidophylla. MX205414B (es)

Priority Applications (6)

Application Number Priority Date Filing Date Title
MX9601719A MX205414B (es) 1996-05-08 1996-05-08 Metodo para incrementar de trehalosa de los organismos por medio de su transformacion con el adnc de la trehalosa-6-fosfato sintasa/fosfatasa de selaginella lepidophylla.
JP9539760A JP2000509602A (ja) 1996-05-08 1997-05-06 セラギネラ・レピドフィラ由来のトレハロース―６―リン酸シンターゼ／ホスファターゼのｃＤＮＡで形質転換することによる、生物中のトレハロース含量の増加法
BR9710436A BR9710436A (pt) 1996-05-08 1997-05-06 Processo para aumentar o teor de trealose em organismos por meio de sua transforma-Æo com o cdna da sintese-fosfatase de trealose-6-fosfato de selaginella lepidophylla
PCT/MX1997/000012 WO1997042327A2 (es) 1996-05-08 1997-05-06 MÉTODO PARA INCREMENTAR EL CONTENIDO DE TREHALOSA DE LOS ORGANISMOS POR MEDIO DE SU TRANSFORMACIÓN CON EL ADNc DE LA TREHALOSA-6-FOSFATO SINTASA/FOSFATASA DE SELAGINELLA LEPIDOPHYLLA
EP97923309A EP0933428A2 (en) 1996-05-08 1997-05-06 METHOD FOR INCREASING THE CONTENT OF TREHALOSE IN ORGANISMS THROUGH THE TRANSFORMATION THEREOF WITH THE cDNA OF THE TREHALOSE-6-PHOSPHATE SYNTHETASE/PHOSPHATASE OF SELAGINELLA LEPIDOPHYLLA
AU29135/97A AU727509B2 (en) 1996-05-08 1997-05-06 Method for increasing the trehalose content in organisms by means of their transformation with the cDNA of trehalose- 6-phosphate synthase/phosphatase from selaginella lepidophylla

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
MX9601719A MX205414B (es) 1996-05-08 1996-05-08 Metodo para incrementar de trehalosa de los organismos por medio de su transformacion con el adnc de la trehalosa-6-fosfato sintasa/fosfatasa de selaginella lepidophylla.

Publications (3)

Publication Number Publication Date
MX9601719A true MX9601719A (es) 1997-11-29
MXPA96001719A MXPA96001719A (es) 1998-07-03
MX205414B MX205414B (es) 2001-12-07

Family

ID=19744918

Family Applications (1)

Application Number Title Priority Date Filing Date
MX9601719A MX205414B (es) 1996-05-08 1996-05-08 Metodo para incrementar de trehalosa de los organismos por medio de su transformacion con el adnc de la trehalosa-6-fosfato sintasa/fosfatasa de selaginella lepidophylla.

Country Status (6)

Country Link
EP (1) EP0933428A2 (es)
JP (1) JP2000509602A (es)
AU (1) AU727509B2 (es)
BR (1) BR9710436A (es)
MX (1) MX205414B (es)
WO (1) WO1997042327A2 (es)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN1997CH00924A (en) 1996-05-03 2005-03-04 Syngenta Mogen Bv Regulating metabolism by modifying the level of trehalose-6-phosphate
EP0868916A3 (en) * 1997-03-04 2004-09-15 Kabushiki Kaisha Hayashibara Seibutsu Kagaku Kenkyujo Reduction inhibitory agent for active-oxygen eliminating activity
AU2412499A (en) * 1997-10-30 1999-05-31 Mogen International N.V. Novel high-fermenting microorganisms
AU1559799A (en) * 1997-10-30 1999-05-24 Mogen International N.V. Novel high-fermenting microorganisms
AU748604B2 (en) * 1998-03-11 2002-06-06 Syngenta Participations Ag Expression of trehalose biosynthetic genes in plants
US7345217B2 (en) 1998-09-22 2008-03-18 Mendel Biotechnology, Inc. Polynucleotides and polypeptides in plants
EP1002867A1 (en) * 1998-10-15 2000-05-24 K.U. Leuven Research & Development Specific genetic modification of the activity of trehalose-6-phosphate synthase and expression in a homologous or heterologous environment
ATE398182T1 (de) * 1999-11-17 2008-07-15 Mendel Biotechnology Inc Toleranzgene für umweltstress
EP2175028A3 (en) * 2000-04-07 2010-07-14 BASF Plant Science GmbH Phosphatase stress-related proteins and methods of use in plants
US8426678B2 (en) 2002-09-18 2013-04-23 Mendel Biotechnology, Inc. Polynucleotides and polypeptides in plants
EP1375669A1 (en) * 2002-06-13 2004-01-02 Stichting Voor De Technische Wetenschappen Method for enhancing the disease resistance in plants by altering trehalose-6-phosphate levels
EP1873247A1 (en) * 2006-06-29 2008-01-02 VIB vzw Novel bifunctional trehalose synthase
ES2590177T3 (es) 2008-02-29 2016-11-18 Monsanto Technology Llc Acontecimiento de planta de maíz MON87460 y composiciones y procedimientos para la detección del mismo
CN103739548B (zh) * 2013-12-27 2015-08-05 南京中医药大学 具有抗真菌活性的化合物及其制备方法与应用

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0451896B1 (en) * 1990-03-28 1996-01-17 Gist-Brocades N.V. New yeast strains with enhanced trehalose content, process to obtain such yeasts and the use of these yeasts
US5422254A (en) * 1992-02-14 1995-06-06 Oy Alko Ab Method to increase the trehalose content of organisms by transforming them with the structural genes for the short and long chains of yeast trehalose synthase
FI943133A0 (fi) * 1994-06-29 1994-06-29 Alko Ab Oy Transgena vaexter
EP0577915A1 (fr) * 1992-07-09 1994-01-12 N.V. Algist-Bruggeman Souches de levures transformées de manière à posséder une résistance au stress et/ou un pouvoir fermentatif amélioré
RO115650B1 (ro) * 1993-06-30 2000-04-28 Mogen Int Acid nucleic, secventa de acid nucleic si vector care determina producerea de trehaloza si procedeu de producere a plantelor cu un continut crescut de trehaloza
IL116564A0 (en) * 1995-01-04 1996-03-31 Mogen Int Process for producing trehalose in plants
EP0784095A3 (en) * 1996-01-12 1997-12-29 Mogen International N.V. Enhanced accummulation of trehalose in plants
IN1997CH00924A (en) * 1996-05-03 2005-03-04 Syngenta Mogen Bv Regulating metabolism by modifying the level of trehalose-6-phosphate

Also Published As

Publication number Publication date
EP0933428A2 (en) 1999-08-04
MX205414B (es) 2001-12-07
AU2913597A (en) 1997-11-26
JP2000509602A (ja) 2000-08-02
WO1997042327A2 (es) 1997-11-13
BR9710436A (pt) 1999-08-17
AU727509B2 (en) 2000-12-14
WO1997042327A3 (es) 1998-01-08

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