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MX2024010670A - Nickasas cas12a. - Google Patents

Nickasas cas12a.

Info

Publication number
MX2024010670A
MX2024010670A MX2024010670A MX2024010670A MX2024010670A MX 2024010670 A MX2024010670 A MX 2024010670A MX 2024010670 A MX2024010670 A MX 2024010670A MX 2024010670 A MX2024010670 A MX 2024010670A MX 2024010670 A MX2024010670 A MX 2024010670A
Authority
MX
Mexico
Prior art keywords
cas12a
nickases
relates
nickase activity
cas12a enzyme
Prior art date
Application number
MX2024010670A
Other languages
English (en)
Inventor
Frank Meulewaeter
Der Oost John Van
Warren Ricardo Villegas
Maartje Janneke Luteijn
Raymond Hubert Josèphe Staals
Wen Ying Wu
Vleesschauwer David De
D''halluin KATELIJN
Original Assignee
BASF Agricultural Solutions Seed US LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BASF Agricultural Solutions Seed US LLC filed Critical BASF Agricultural Solutions Seed US LLC
Publication of MX2024010670A publication Critical patent/MX2024010670A/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

La presente invención se refiere al campo de la edición del genoma génico. En particular, se refiere a la provisión de una enzima Cas12a que tiene actividad de nickasa, así como también a los medios y métodos para la modificación de un locus genómico de interés con una enzima Cas12a que tiene actividad de nickasa y usos de estos.
MX2024010670A 2022-03-01 2023-03-01 Nickasas cas12a. MX2024010670A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP22159465 2022-03-01
EP22202125 2022-10-18
PCT/EP2023/055134 WO2023166030A1 (en) 2022-03-01 2023-03-01 Cas12a nickases

Publications (1)

Publication Number Publication Date
MX2024010670A true MX2024010670A (es) 2024-09-11

Family

ID=85384366

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2024010668A MX2024010668A (es) 2022-03-01 2023-03-01 Nickasas cas12a.
MX2024010670A MX2024010670A (es) 2022-03-01 2023-03-01 Nickasas cas12a.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2024010668A MX2024010668A (es) 2022-03-01 2023-03-01 Nickasas cas12a.

Country Status (12)

Country Link
US (1) US20230374480A1 (es)
EP (3) EP4486878A1 (es)
JP (2) JP2025507834A (es)
KR (2) KR20240153594A (es)
AR (3) AR128679A1 (es)
AU (2) AU2023227365A1 (es)
CA (2) CA3245344A1 (es)
CL (2) CL2024002606A1 (es)
IL (2) IL315220A (es)
MX (2) MX2024010668A (es)
TW (3) TW202342754A (es)
WO (3) WO2023166032A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025045884A1 (en) * 2023-08-31 2025-03-06 Basf Agricultural Solutions Us Llc Class 2 type v crispr-cas prime editing

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE57390T1 (de) 1986-03-11 1990-10-15 Plant Genetic Systems Nv Durch gentechnologie erhaltene und gegen glutaminsynthetase-inhibitoren resistente pflanzenzellen.
DE69125991T2 (de) 1990-04-04 1997-09-25 Pioneer Hi Bred Int Herstellung von rapssamen mit verringertem gehalt an gesättigten fettsäuren
FR2673642B1 (fr) 1991-03-05 1994-08-12 Rhone Poulenc Agrochimie Gene chimere comprenant un promoteur capable de conferer a une plante une tolerance accrue au glyphosate.
FR2673643B1 (fr) 1991-03-05 1993-05-21 Rhone Poulenc Agrochimie Peptide de transit pour l'insertion d'un gene etranger dans un gene vegetal et plantes transformees en utilisant ce peptide.
US6509516B1 (en) 1999-10-29 2003-01-21 Plant Genetic Systems N.V. Male-sterile brassica plants and methods for producing same
US6506963B1 (en) 1999-12-08 2003-01-14 Plant Genetic Systems, N.V. Hybrid winter oilseed rape and methods for producing same
PL2167667T3 (pl) 2007-07-09 2015-10-30 Bayer Cropscience Nv Roślina Brassica zawierająca zmutowane allele tłuszczowej tioesterazy acylo-ACP
PL2220239T3 (pl) 2007-11-28 2015-10-30 Bayer Cropscience Nv Roślina Brassica zawierająca zmutowane allele typu indehiscent
PL2304038T3 (pl) 2008-07-17 2017-03-31 Bayer Cropscience Nv Roślina Brassica zawierająca zmutowane allele typu INDEHISCENT
PL2501804T3 (pl) 2009-11-20 2016-11-30 Rośliny Brassica zawierające zmutowane allele FAD3
EP4166662A1 (en) 2016-04-29 2023-04-19 BASF Plant Science Company GmbH Methods for modification of target nucleic acids using fused guide rna - donor molecules
US11168313B2 (en) 2016-07-26 2021-11-09 The General Hospital Corporation Variants of CRISPR from Prevotella and Francisella 1 (Cpf1)
WO2018195545A2 (en) 2017-04-21 2018-10-25 The General Hospital Corporation Variants of cpf1 (cas12a) with altered pam specificity
WO2018213726A1 (en) * 2017-05-18 2018-11-22 The Broad Institute, Inc. Systems, methods, and compositions for targeted nucleic acid editing
US20210079366A1 (en) * 2017-12-22 2021-03-18 The Broad Institute, Inc. Cas12a systems, methods, and compositions for targeted rna base editing
CN112424349A (zh) 2018-06-04 2021-02-26 哥本哈根大学 突变的Cpf1核酸内切酶
BR112021002258A2 (pt) 2018-08-08 2021-05-04 Integrated Dna Technologies, Inc. proteína associada a crispr, complexo de ribonucleoproteína crispr, métodos para aumentar a eficiência de edição de genes em sítios tttn pam, para aumentar a eficiência de edição de genes em sítios não canônicos de tttt pam e para realizar edição do genoma em uma célula eucariótica, kit, ácido nucleico, sequência polinucleotídica que codifica um polipeptídeo cas12a, sequência de aminoácidos que codifica um polipeptídeo cas12a, e, sistema de endonuclease cas.
CN114127285B (zh) * 2019-03-19 2024-09-10 布罗德研究所股份有限公司 编辑核苷酸序列的方法和组合物
WO2021081367A1 (en) * 2019-10-23 2021-04-29 Pairwise Plants Services, Inc. Compositions and methods for rna-templated editing in plants
AU2020410138A1 (en) 2019-12-16 2022-06-23 BASF Agricultural Solutions Seed US LLC Precise introduction of DNA or mutations into the genome of wheat
CA3161254A1 (en) 2019-12-16 2021-06-24 Katelijn D'HALLUIN Improved genome editing using paired nickases
KR20220150328A (ko) 2020-03-04 2022-11-10 바스프 에스이 낮은 내지 중간 발현을 부여하는 구성적 박테리아 프로모터를 생산하는 방법
WO2021222703A2 (en) * 2020-05-01 2021-11-04 Integrated Dna Technologies, Inc. Lachnospiraceae sp. cas12a mutants with enhanced cleavage activity at non-canonical tttt protospacer adjacent motifs

Also Published As

Publication number Publication date
AU2023227365A1 (en) 2024-09-12
AR128679A1 (es) 2024-06-05
WO2023166029A1 (en) 2023-09-07
CA3245344A1 (en) 2023-09-07
JP2025507908A (ja) 2025-03-21
EP4486879A1 (en) 2025-01-08
WO2023166032A1 (en) 2023-09-07
AR128678A1 (es) 2024-06-05
KR20240149443A (ko) 2024-10-14
MX2024010668A (es) 2024-09-11
CL2024002606A1 (es) 2025-01-17
TW202342744A (zh) 2023-11-01
CA3245339A1 (en) 2023-09-07
EP4486877A1 (en) 2025-01-08
KR20240153594A (ko) 2024-10-23
IL315220A (en) 2024-10-01
TW202342756A (zh) 2023-11-01
TW202342754A (zh) 2023-11-01
AU2023228036A1 (en) 2024-09-12
EP4486878A1 (en) 2025-01-08
AR128680A1 (es) 2024-06-05
WO2023166030A1 (en) 2023-09-07
IL315225A (en) 2024-10-01
CL2024002604A1 (es) 2025-01-17
JP2025507834A (ja) 2025-03-21
US20230374480A1 (en) 2023-11-23

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