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MX2024007771A - Productos y metodos para la expresion heterologa de proteinas en una celula hospedera. - Google Patents

Productos y metodos para la expresion heterologa de proteinas en una celula hospedera.

Info

Publication number
MX2024007771A
MX2024007771A MX2024007771A MX2024007771A MX2024007771A MX 2024007771 A MX2024007771 A MX 2024007771A MX 2024007771 A MX2024007771 A MX 2024007771A MX 2024007771 A MX2024007771 A MX 2024007771A MX 2024007771 A MX2024007771 A MX 2024007771A
Authority
MX
Mexico
Prior art keywords
methods
proteins
products
host cell
heterologous expression
Prior art date
Application number
MX2024007771A
Other languages
English (en)
Inventor
Ariel Schwartz
Gregory Hannum
Miles Gander
Amy E Doerner
Carla Risso
Original Assignee
Absci Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Absci Corp filed Critical Absci Corp
Publication of MX2024007771A publication Critical patent/MX2024007771A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y111/00—Oxidoreductases acting on a peroxide as acceptor (1.11)
    • C12Y111/01—Peroxidases (1.11.1)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La descripción proporciona productos y métodos para la expresión heteróloga de proteínas en células hospederas. Los constructos de expresión, las células bacterianas y los métodos proporcionados producen una proteína accesoria PnlP-1 que aumenta el rendimiento de una proteína heteróloga de interés.
MX2024007771A 2021-12-23 2022-12-13 Productos y metodos para la expresion heterologa de proteinas en una celula hospedera. MX2024007771A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202163293285P 2021-12-23 2021-12-23
PCT/US2022/081429 WO2023122448A1 (en) 2021-12-23 2022-12-13 Products and methods for heterologous expression of proteins in a host cell

Publications (1)

Publication Number Publication Date
MX2024007771A true MX2024007771A (es) 2024-07-01

Family

ID=86903629

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2024007771A MX2024007771A (es) 2021-12-23 2022-12-13 Productos y metodos para la expresion heterologa de proteinas en una celula hospedera.

Country Status (9)

Country Link
US (1) US20250051823A1 (es)
EP (1) EP4453198A1 (es)
JP (1) JP2024545466A (es)
CN (1) CN118434853A (es)
AU (1) AU2022420471A1 (es)
CA (1) CA3240314A1 (es)
IL (1) IL313160A (es)
MX (1) MX2024007771A (es)
WO (1) WO2023122448A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20250109209A1 (en) 2023-10-03 2025-04-03 Absci Corporation Tl1a associated antibody compositions and methods of use
WO2025122885A1 (en) 2023-12-08 2025-06-12 Absci Corporation Anti-her2 associated antibody compositions designed by artificial intelligence and methods of use

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003076637A1 (en) * 2002-03-13 2003-09-18 Degussa Ag Process for the preparation of l-amino acids using strains of the family enterobacteriaceae
US8178338B2 (en) 2005-07-01 2012-05-15 The Regents Of The University Of California Inducible expression vectors and methods of use thereof
KR101589554B1 (ko) 2008-01-03 2016-02-01 코넬 리서치 파운데이션 인코포레이티드 원핵생물에서의 글리코실화 단백질 발현
US20150353940A1 (en) 2013-08-05 2015-12-10 Absci, Llc Vectors for use in an inducible coexpression system
WO2017106583A1 (en) 2015-12-15 2017-06-22 Absci, Llc Cytoplasmic expression system
WO2021146626A1 (en) 2020-01-15 2021-07-22 Absci Llc Activity-specific cell enrichment
CA3170531A1 (en) 2020-02-11 2021-08-19 Absci Corporation Proximity assay

Also Published As

Publication number Publication date
WO2023122448A1 (en) 2023-06-29
CN118434853A (zh) 2024-08-02
AU2022420471A1 (en) 2024-07-18
CA3240314A1 (en) 2023-06-29
EP4453198A1 (en) 2024-10-30
JP2024545466A (ja) 2024-12-06
US20250051823A1 (en) 2025-02-13
IL313160A (en) 2024-07-01

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