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MX2024007508A - Tamizaje en fase solida de cepas bacterianas de alto rendimiento. - Google Patents

Tamizaje en fase solida de cepas bacterianas de alto rendimiento.

Info

Publication number
MX2024007508A
MX2024007508A MX2024007508A MX2024007508A MX2024007508A MX 2024007508 A MX2024007508 A MX 2024007508A MX 2024007508 A MX2024007508 A MX 2024007508A MX 2024007508 A MX2024007508 A MX 2024007508A MX 2024007508 A MX2024007508 A MX 2024007508A
Authority
MX
Mexico
Prior art keywords
solid
phase screening
bacterial strains
performing bacterial
present disclosure
Prior art date
Application number
MX2024007508A
Other languages
English (en)
Inventor
Jia Liu
Original Assignee
Absci Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Absci Corp filed Critical Absci Corp
Publication of MX2024007508A publication Critical patent/MX2024007508A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6881—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1072—Differential gene expression library synthesis, e.g. subtracted libraries, differential screening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente descripción proporciona composiciones y métodos para identificar una célula huésped o células huésped de una población de células que producen un producto génico de interés. La presente descripción proporciona además un flujo de trabajo de tamizaje en fase sólida que permite la determinación de la afinidad y la identificación de cepas de alta producción.
MX2024007508A 2021-12-17 2022-12-16 Tamizaje en fase solida de cepas bacterianas de alto rendimiento. MX2024007508A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US202163291059P 2021-12-17 2021-12-17
PCT/US2022/053107 WO2023114452A1 (en) 2021-12-17 2022-12-16 Solid-phase screening for high-performing bacterial strains

Publications (1)

Publication Number Publication Date
MX2024007508A true MX2024007508A (es) 2024-07-09

Family

ID=85157309

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2024007508A MX2024007508A (es) 2021-12-17 2022-12-16 Tamizaje en fase solida de cepas bacterianas de alto rendimiento.

Country Status (9)

Country Link
US (1) US20250051760A1 (es)
EP (1) EP4448757A1 (es)
JP (1) JP2024543732A (es)
CN (1) CN118475694A (es)
AU (1) AU2022409506A1 (es)
CA (1) CA3242967A1 (es)
IL (1) IL313521A (es)
MX (1) MX2024007508A (es)
WO (1) WO2023114452A1 (es)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024254237A1 (en) * 2023-06-08 2024-12-12 Absci Corporation Solid-phase screening for high-performing bacterial strains

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5834247A (en) * 1992-12-09 1998-11-10 New England Biolabs, Inc. Modified proteins comprising controllable intervening protein sequences or their elements methods of producing same and methods for purification of a target protein comprised by a modified protein
JP4730804B2 (ja) * 2000-01-31 2011-07-20 センス・プロテオミック・リミテッド 方法
WO2007005837A2 (en) 2005-07-01 2007-01-11 The Regents Of The University Of California Inducible expression vectors and methods of use thereof
JP5667880B2 (ja) 2008-01-03 2015-02-12 コーネル リサーチ ファンデーション インコーポレイテッド 原核生物におけるグリコシル化されたタンパク質の発現方法
US20150353940A1 (en) 2013-08-05 2015-12-10 Absci, Llc Vectors for use in an inducible coexpression system
WO2017106583A1 (en) 2015-12-15 2017-06-22 Absci, Llc Cytoplasmic expression system

Also Published As

Publication number Publication date
CN118475694A (zh) 2024-08-09
IL313521A (en) 2024-08-01
AU2022409506A1 (en) 2024-07-11
CA3242967A1 (en) 2023-06-22
EP4448757A1 (en) 2024-10-23
WO2023114452A1 (en) 2023-06-22
US20250051760A1 (en) 2025-02-13
JP2024543732A (ja) 2024-11-22

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