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MX2024000814A - Metodos de elaboracion de perfiles especificos al tipo celular para identificar objetivos de farmacos. - Google Patents

Metodos de elaboracion de perfiles especificos al tipo celular para identificar objetivos de farmacos.

Info

Publication number
MX2024000814A
MX2024000814A MX2024000814A MX2024000814A MX2024000814A MX 2024000814 A MX2024000814 A MX 2024000814A MX 2024000814 A MX2024000814 A MX 2024000814A MX 2024000814 A MX2024000814 A MX 2024000814A MX 2024000814 A MX2024000814 A MX 2024000814A
Authority
MX
Mexico
Prior art keywords
cell
methods
type specific
drug targets
identify drug
Prior art date
Application number
MX2024000814A
Other languages
English (en)
Inventor
Xiao Xu
Nathaniel Heintz
Original Assignee
Univ Rockefeller
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Rockefeller filed Critical Univ Rockefeller
Publication of MX2024000814A publication Critical patent/MX2024000814A/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0619—Neurons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6841—In situ hybridisation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/02—Drug screening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/136—Screening for pharmacological compounds

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Neurology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Neurosurgery (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Toxicology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La presente invención proporciona métodos para elaborar perfiles de expresión génica y de proteína de múltiples núcleos de un único tipo celular y comparar los perfiles para determinar la variabilidad entre las poblaciones celulares, las muestras de diferentes sujetos 5 y las células que expresan un fenotipo de enfermedad.
MX2024000814A 2017-02-10 2019-08-12 Metodos de elaboracion de perfiles especificos al tipo celular para identificar objetivos de farmacos. MX2024000814A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762457420P 2017-02-10 2017-02-10
US201762558396P 2017-09-14 2017-09-14

Publications (1)

Publication Number Publication Date
MX2024000814A true MX2024000814A (es) 2024-02-06

Family

ID=63107837

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2019009612A MX2019009612A (es) 2017-02-10 2018-02-09 Metodos de elaboracion de perfiles especificos al tipo celular para identificar objetivos de farmacos.
MX2024000814A MX2024000814A (es) 2017-02-10 2019-08-12 Metodos de elaboracion de perfiles especificos al tipo celular para identificar objetivos de farmacos.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2019009612A MX2019009612A (es) 2017-02-10 2018-02-09 Metodos de elaboracion de perfiles especificos al tipo celular para identificar objetivos de farmacos.

Country Status (10)

Country Link
EP (2) EP3580338B1 (es)
JP (3) JP7229538B2 (es)
CN (2) CN110582578B (es)
AU (2) AU2018219308B2 (es)
CA (1) CA3052290A1 (es)
ES (1) ES2992087T3 (es)
IL (1) IL268417B2 (es)
MX (2) MX2019009612A (es)
SG (1) SG11201907162TA (es)
WO (1) WO2018148501A1 (es)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3746566A1 (en) * 2018-01-31 2020-12-09 Dovetail Genomics, LLC Sample prep for dna linkage recovery
WO2020037311A1 (en) * 2018-08-17 2020-02-20 University Of Florida Research Foundation, Inc. Protein and peptide biomarkers for traumatic injury to the central nervous system
TWI852977B (zh) 2019-01-10 2024-08-21 美商健生生物科技公司 前列腺新抗原及其用途
JP2022544496A (ja) * 2019-08-12 2022-10-19 ミッション バイオ インコーポレイテッド 同一のシングルセルにおける、タンパク質発現、一塩基変化、及びコピー数多型のマルチオミクス同時検出のための方法、システム、及び装置
KR20220052946A (ko) * 2019-09-06 2022-04-28 각고호우징 게이오기주크 글리아 전구 세포를 포함하는 세포 응집체의 제조 방법
US11781138B2 (en) 2019-10-14 2023-10-10 Aro Biotherapeutics Company FN3 domain-siRNA conjugates and uses thereof
EP4045061A4 (en) 2019-10-14 2024-04-17 ARO Biotherapeutics Company CD71-BINDING FIBRONECTIN TYPE III DOMAINS
US12491255B2 (en) 2019-10-14 2025-12-09 Aro Biotherapeutics Company EPCAM binding fibronectin type III domains
CN110699362B (zh) * 2019-11-15 2023-03-24 兰州理工大学 Afp5基因及其应用
CN111312330A (zh) * 2020-02-13 2020-06-19 兰州理工大学 一种基于蛋白质节点特性的关键蛋白质识别方法及系统
WO2021226389A2 (en) * 2020-05-06 2021-11-11 Dignity Health Systems and methods for treating levodopa dyskinesia, enhancing motor benefit, and delaying disease progression
EP4175664A2 (en) 2020-07-06 2023-05-10 Janssen Biotech, Inc. Prostate neoantigens and their uses
CN112538480B (zh) * 2020-12-24 2022-09-27 广州市妇女儿童医疗中心（广州市妇幼保健院、广州市儿童医院、广州市妇婴医院、广州市妇幼保健计划生育服务中心） 精子鞭毛多发形态异常疾病动物模型的构建方法和应用
CN112522425B (zh) * 2020-12-25 2023-11-28 武汉睿健医药科技有限公司 Dpc细胞鉴定分类用标记物基因、筛选方法及应用
CN112481273B (zh) * 2020-12-29 2023-11-24 南通大学附属医院 结直肠癌抑癌基因及其启动子区高dna甲基化的验证方法
EP4323409A4 (en) 2021-04-14 2025-04-16 ARO Biotherapeutics Company CD71-BINDING FIBRONECTIN TYPE III DOMAINS
CA3215367A1 (en) 2021-04-14 2022-10-20 Swapnil Kulkarni Fn3 domain-sirna conjugates and uses thereof
CN113558011B (zh) * 2021-07-23 2022-05-10 深圳市中医院 基于γ-分泌酶激活蛋白基因的干燥综合征动物模型的建立方法
CN113893892B (zh) * 2021-11-10 2023-02-10 大连海事大学 一种基于微流控芯片的纳米孔及其制备方法
CN114276993A (zh) * 2021-11-11 2022-04-05 上海昆盟生物科技有限公司 一种用于分离大鼠下丘脑弓状核神经元的方法
CN114457075B (zh) * 2021-12-15 2023-05-02 徐州市中心医院 一种敲低PXYLP1基因表达的shRNA、慢病毒载体及其构建方法和应用
CN114751990B (zh) * 2022-04-01 2023-10-20 中国科学技术大学 酰胺高丝氨酸内酯受体-增强绿色荧光融合蛋白及其制备方法与应用
JP2025516321A (ja) * 2022-05-05 2025-05-27 アルナイラム ファーマシューティカルズ， インコーポレイテッド アクチン結合ＬＩＭタンパク質３（ＡＢＬＩＭ３）ｉＲＮＡ剤組成物およびその使用方法
CN115083522B (zh) * 2022-08-18 2022-10-28 天津诺禾致源生物信息科技有限公司 细胞类型的预测方法、装置及服务器
CN116004773B (zh) * 2022-09-30 2025-04-08 华南农业大学 一种线性置换等温扩增方法及其应用
CN115927596A (zh) * 2022-12-02 2023-04-07 中国科学技术大学 一种用于诊断小鼠小脑衰老的生物标记物及其应用
CN119614700B (zh) * 2025-02-13 2025-06-10 南昌大学第一附属医院 MybpC1基因/蛋白在制备骨关节炎药物中的应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2009203785A1 (en) * 2008-01-04 2009-07-16 Centre National De La Recherche Scientifique Molecular in vitro diagnosis of breast cancer
US20110071049A1 (en) * 2008-03-12 2011-03-24 Nathaniel Heintz Methods and compositions for translational profiling and molecular phenotyping
WO2012105854A2 (en) 2011-01-31 2012-08-09 Curonz Holdings Company Limited Compositions for administration to the eye
EP3031929A1 (en) * 2014-12-11 2016-06-15 Mdc Max-Delbrück-Centrum Für Molekulare Medizin Berlin - Buch Genome architecture mapping

Also Published As

Publication number Publication date
AU2018219308B2 (en) 2024-08-08
AU2024259851A1 (en) 2024-12-12
CN117965689A (zh) 2024-05-03
JP2025032204A (ja) 2025-03-11
EP3580338B1 (en) 2024-09-11
CA3052290A1 (en) 2018-08-16
IL268417B1 (en) 2025-01-01
EP3580338A4 (en) 2021-05-26
IL268417B2 (en) 2025-05-01
WO2018148501A1 (en) 2018-08-16
JP2023058610A (ja) 2023-04-25
SG11201907162TA (en) 2019-09-27
JP2020508652A (ja) 2020-03-26
CN110582578A (zh) 2019-12-17
EP3580338A1 (en) 2019-12-18
EP4461818A2 (en) 2024-11-13
IL268417A (en) 2019-09-26
AU2018219308A1 (en) 2019-08-22
EP3580338C0 (en) 2024-09-11
MX2019009612A (es) 2019-11-12
ES2992087T3 (es) 2024-12-09
JP7229538B2 (ja) 2023-03-01
CN110582578B (zh) 2024-02-02
EP4461818A3 (en) 2025-02-12
JP7604014B2 (ja) 2024-12-23

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