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MX2022013044A - Glucoamilasa y métodos de uso de esta. - Google Patents

Glucoamilasa y métodos de uso de esta.

Info

Publication number
MX2022013044A
MX2022013044A MX2022013044A MX2022013044A MX2022013044A MX 2022013044 A MX2022013044 A MX 2022013044A MX 2022013044 A MX2022013044 A MX 2022013044A MX 2022013044 A MX2022013044 A MX 2022013044A MX 2022013044 A MX2022013044 A MX 2022013044A
Authority
MX
Mexico
Prior art keywords
glucoamylase
methods
producing
mucorales
clade
Prior art date
Application number
MX2022013044A
Other languages
English (en)
Inventor
Chao Huang
Jing Ge
Zhongmei Tang
Zhenghong Zhang
Helong Hao
Lilia Babe
Xingxiang Xi
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Publication of MX2022013044A publication Critical patent/MX2022013044A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
    • A23L2/38—Other non-alcoholic beverages
    • A23L2/382—Other non-alcoholic beverages fermented
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/06—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
    • A23L29/35—Degradation products of starch, e.g. hydrolysates, dextrins; Enzymatically modified starches
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Nutrition Science (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

Se describen una célula hospedera recombinante, una composición que comprende una glucoamilasa y métodos para sacarificar el sustrato de almidón usando la glucoamilasa; además, la divulgación también se relaciona con un proceso para producir productos de fermentación y un método para incrementar la digestibilidad del almidón en un animal, así como un método para producir una bebida fermentada; la glucoamilasa o 1,4-alfa-D-glucano glucohidrolasa (EC 3.2.1.3) es preferiblemente del clado de Mucorales, especialmente que tiene alta identidad de secuencia con la glucoamilasa de Saksenaea vasiformis.
MX2022013044A 2020-04-17 2021-04-19 Glucoamilasa y métodos de uso de esta. MX2022013044A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN2020085393 2020-04-17
PCT/US2021/027894 WO2021212095A1 (en) 2020-04-17 2021-04-19 Glucoamylase and methods of use thereof

Publications (1)

Publication Number Publication Date
MX2022013044A true MX2022013044A (es) 2023-01-24

Family

ID=75870748

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022013044A MX2022013044A (es) 2020-04-17 2021-04-19 Glucoamilasa y métodos de uso de esta.

Country Status (9)

Country Link
US (2) US12509670B2 (es)
EP (1) EP4136222A1 (es)
JP (2) JP7730339B2 (es)
KR (1) KR20230002730A (es)
CN (1) CN115867651A (es)
AU (1) AU2021255739B2 (es)
CA (1) CA3175633A1 (es)
MX (1) MX2022013044A (es)
WO (1) WO2021212095A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7730339B2 (ja) * 2020-04-17 2025-08-27 ダニスコ・ユーエス・インク グルコアミラーゼ及びその使用方法
CN119487187A (zh) * 2021-10-15 2025-02-18 丹尼斯科美国公司 葡糖淀粉酶变体及其使用方法
EP4486873A1 (en) * 2021-12-24 2025-01-08 Danisco US Inc. Hybrid glucoamylases and methods of use thereof
EP4525615A2 (en) 2022-05-14 2025-03-26 Novozymes A/S Compositions and methods for preventing, treating, supressing and/or eliminating phytopathogenic infestations and infections
WO2024137350A2 (en) 2022-12-19 2024-06-27 Danisco Us Inc. Recombinant fungal strains and methods thereof for producing consistent proteins
CN115976133A (zh) * 2022-12-30 2023-04-18 泉后(广州)生物科技研究院有限公司 以禾本科种籽为原料多酶体系催化制备植物提取物的方法

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61141890A (ja) * 1984-12-15 1986-06-28 Suntory Ltd アルコ−ルの製造法
US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
AU639570B2 (en) 1990-05-09 1993-07-29 Novozymes A/S A cellulase preparation comprising an endoglucanase enzyme
IL99552A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof
ES2182818T5 (es) 1990-12-10 2015-07-06 Danisco Us Inc. Sacarificación mejorada de celulosa por clonación y amplificación del gen de beta-glucosidasa de Trichoderma reesei
DK13491D0 (da) 1991-01-25 1991-01-25 Novo Nordisk As Anvendelse af et enzymholdigt granulat og fremgangsmaade til fremstilling af et forderstof i tabletform
DE4343591A1 (de) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
AU7489896A (en) 1995-11-02 1997-05-22 Novo Nordisk A/S Feed enzyme preparations
ATE519842T1 (de) 2004-11-30 2011-08-15 Genencor Int Trichoderma reesei-glucoamylase und homologe davon
PL1940241T3 (pl) 2005-10-12 2013-01-31 Genencor Int Stabilne, trwałe granulki z substancjami czynnymi
US20080280328A1 (en) 2005-11-18 2008-11-13 Novozymes A/S Glucoamylase Variants
US8592194B2 (en) 2007-10-09 2013-11-26 Danisco Us Inc. Glucoamylase variants with altered properties
CA2807558A1 (en) 2010-08-06 2012-02-09 Danisco Us Inc. Production of isoprene under neutral ph conditions
JP2015518707A (ja) 2012-05-11 2015-07-06 ダニスコ・ユーエス・インク 糖化のための、ａｓｐｅｒｇｉｌｌｕｓｃｌａｖａｔｕｓ由来アルファアミラーゼの使用
US20160010128A1 (en) 2012-12-20 2016-01-14 Danisco Us Inc. Method of using alpha-amylase from aspergillus terreus and pullulanase for saccharification
US10294466B2 (en) 2015-02-25 2019-05-21 Danisco Us Inc. Alpha-glucosidase, compositions and methods
CN109072266B (zh) 2015-12-21 2023-06-23 丹尼斯科美国公司 改善的颗粒状淀粉转化酶和方法
CN109661403A (zh) * 2016-08-05 2019-04-19 嘉吉公司 前导序列修饰的葡糖淀粉酶多肽和具有增强的生物产物产生的工程化的酵母菌株
MX2020009344A (es) 2018-03-09 2020-11-13 Danisco Us Inc Glucoamilasas y métodos para su uso.
WO2019191263A1 (en) 2018-03-27 2019-10-03 Cargill, Incorporated Methods for ethanol production using engineered yeast
JP7730339B2 (ja) * 2020-04-17 2025-08-27 ダニスコ・ユーエス・インク グルコアミラーゼ及びその使用方法

Also Published As

Publication number Publication date
WO2021212095A1 (en) 2021-10-21
US12509670B2 (en) 2025-12-30
AU2021255739B2 (en) 2025-10-16
US20230340444A1 (en) 2023-10-26
JP2023527266A (ja) 2023-06-28
AU2021255739A1 (en) 2022-11-17
EP4136222A1 (en) 2023-02-22
KR20230002730A (ko) 2023-01-05
US20220396783A1 (en) 2022-12-15
CN115867651A (zh) 2023-03-28
JP2025166188A (ja) 2025-11-05
JP7730339B2 (ja) 2025-08-27
CA3175633A1 (en) 2021-10-21

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