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MX2022006018A - Nanoporos artificiales y usos y metodos relacionados con los mismos. - Google Patents

Nanoporos artificiales y usos y metodos relacionados con los mismos.

Info

Publication number
MX2022006018A
MX2022006018A MX2022006018A MX2022006018A MX2022006018A MX 2022006018 A MX2022006018 A MX 2022006018A MX 2022006018 A MX2022006018 A MX 2022006018A MX 2022006018 A MX2022006018 A MX 2022006018A MX 2022006018 A MX2022006018 A MX 2022006018A
Authority
MX
Mexico
Prior art keywords
methods relating
nanopores
artificial
forming protein
artificial nanopores
Prior art date
Application number
MX2022006018A
Other languages
English (en)
Inventor
Giovanni Maglia
Shengli Zhang
Original Assignee
Univ Groningen
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Groningen filed Critical Univ Groningen
Publication of MX2022006018A publication Critical patent/MX2022006018A/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y15/00—Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/60—Detection means characterised by use of a special device
    • C12Q2565/631—Detection means characterised by use of a special device being a biochannel or pore

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Nanotechnology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • General Physics & Mathematics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Food Science & Technology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La invención se relaciona con el campo de los nanoporos y su uso en el análisis de biopolímeros, incluidos polipéptidos y polinucleótidos. Se proporciona un nanoporo artificial que comprende un conjunto multimérico de subunidades, cada una de las cuales comprende (i) la secuencia transmembrana (TM) de una proteína formadora de poros barril ß o a-helicoidal fusionada con la secuencia de aminoácidos de (ii) una subunidad de una proteína formadora de anillos capaz de controlar el transporte de un polipéptido o polinucleótido a través de la región TM del conjunto.
MX2022006018A 2019-11-19 2020-11-19 Nanoporos artificiales y usos y metodos relacionados con los mismos. MX2022006018A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP19210168 2019-11-19
PCT/NL2020/050726 WO2021101378A1 (en) 2019-11-19 2020-11-19 Artificial nanopores and uses and methods relating thereto

Publications (1)

Publication Number Publication Date
MX2022006018A true MX2022006018A (es) 2022-09-12

Family

ID=68840857

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022006018A MX2022006018A (es) 2019-11-19 2020-11-19 Nanoporos artificiales y usos y metodos relacionados con los mismos.

Country Status (11)

Country Link
US (2) US20220412948A1 (es)
EP (1) EP4061965A1 (es)
JP (1) JP2023502658A (es)
KR (1) KR20220100901A (es)
CN (1) CN114981450A (es)
AU (1) AU2020389020A1 (es)
BR (1) BR112022009402A2 (es)
CA (1) CA3161981A1 (es)
IL (1) IL293024A (es)
MX (1) MX2022006018A (es)
WO (1) WO2021101378A1 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA3111488A1 (en) 2018-09-11 2020-03-19 Rijksuniversiteit Groningen Biological nanopores having tunable pore diameters and uses thereof as analytical tools
CN119213145A (zh) * 2022-05-27 2024-12-27 深圳华大生命科学研究院 一种维持纳米孔测序速度的方法
KR20250096814A (ko) 2022-10-28 2025-06-27 리엑스유니버시테이트 그로닝겐 단일-분자 중합체 프로파일링을 위한 나노기공 시스템 및 방법
KR20250116014A (ko) 2022-10-28 2025-07-31 리엑스유니버시테이트 그로닝겐 나노기공-기반 단백질 분석
CN117417421B (zh) * 2023-01-12 2024-07-23 北京普译生物科技有限公司 一种突变体膜蛋白复合物纳米孔及其应用
CN121013859A (zh) * 2023-03-30 2025-11-25 格罗宁根大学 大圆锥形的纳米孔及其在分析物感测中的应用
WO2024222911A1 (zh) * 2023-04-28 2024-10-31 清华大学 一种被修饰的多肽、多肽复合物、多肽混合物及控制多肽移位速度或表征多肽的系统和方法
WO2025163245A1 (en) * 2024-01-29 2025-08-07 Oulun Yliopisto Protein sequencing apparatus and method
CN119269595B (zh) * 2024-11-14 2025-05-13 中国科学院重庆绿色智能技术研究院 炭疽病毒蛋白纳米孔电化学检测分析装置及其应用

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2453377A (en) * 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
CN102245760A (zh) * 2008-07-07 2011-11-16 牛津纳米孔技术有限公司 酶-孔构建体
WO2013123379A2 (en) * 2012-02-16 2013-08-22 The Regents Of The University Of California Nanopore sensor for enzyme-mediated protein translocation
FR3003268B1 (fr) 2013-03-13 2018-01-19 Roquette Freres Bioreacteur
GB201316849D0 (en) * 2013-09-23 2013-11-06 Isis Innovation Method
AU2014354726B2 (en) * 2013-11-26 2020-03-12 Illumina, Inc. Compositions and methods for polynucleotide sequencing
WO2017098322A1 (en) * 2015-12-08 2017-06-15 Katholieke Universiteit Leuven Ku Leuven Research & Development Modified nanopores, compositions comprising the same, and uses thereof
WO2017149316A1 (en) * 2016-03-02 2017-09-08 Oxford Nanopore Technologies Limited Mutant pore
EP3828280A1 (en) 2016-07-12 2021-06-02 Rijksuniversiteit Groningen Biological nanopores for biopolymer sensing and sequencing based on frac actinoporin
GB201809323D0 (en) * 2018-06-06 2018-07-25 Oxford Nanopore Tech Ltd Method
GB202313202D0 (en) * 2023-08-30 2023-10-11 Portal Biotech Ltd Systems and methods for nanopore-based analyte detection

Also Published As

Publication number Publication date
KR20220100901A (ko) 2022-07-18
US20220412948A1 (en) 2022-12-29
CA3161981A1 (en) 2021-05-27
CN114981450A (zh) 2022-08-30
BR112022009402A2 (pt) 2022-08-09
JP2023502658A (ja) 2023-01-25
EP4061965A1 (en) 2022-09-28
US20240288416A1 (en) 2024-08-29
IL293024A (en) 2022-07-01
WO2021101378A1 (en) 2021-05-27
AU2020389020A1 (en) 2022-06-09

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