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MX2022004356A - Metodos de cromatografia de liquidos-espectrometria de masas (lc-ms) para analizar la variabilidad entre lotes de anfolitos. - Google Patents

Metodos de cromatografia de liquidos-espectrometria de masas (lc-ms) para analizar la variabilidad entre lotes de anfolitos.

Info

Publication number
MX2022004356A
MX2022004356A MX2022004356A MX2022004356A MX2022004356A MX 2022004356 A MX2022004356 A MX 2022004356A MX 2022004356 A MX2022004356 A MX 2022004356A MX 2022004356 A MX2022004356 A MX 2022004356A MX 2022004356 A MX2022004356 A MX 2022004356A
Authority
MX
Mexico
Prior art keywords
methods
mass spectrometry
ampholites
variability
batches
Prior art date
Application number
MX2022004356A
Other languages
English (en)
Inventor
Clare Ryan
Rachel Mullen
Seamus O''connor
Stacey Helming
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of MX2022004356A publication Critical patent/MX2022004356A/es

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44795—Isoelectric focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44747—Composition of gel or of carrier mixture
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
    • G01N27/622—Ion mobility spectrometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/62—Detectors specially adapted therefor
    • G01N30/72—Mass spectrometers
    • G01N30/7233—Mass spectrometers interfaced to liquid or supercritical fluid chromatograph
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N30/86—Signal analysis
    • G01N30/8624—Detection of slopes or peaks; baseline correction
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16C—COMPUTATIONAL CHEMISTRY; CHEMOINFORMATICS; COMPUTATIONAL MATERIALS SCIENCE
    • G16C20/00—Chemoinformatics, i.e. ICT specially adapted for the handling of physicochemical or structural data of chemical particles, elements, compounds or mixtures
    • G16C20/20—Identification of molecular entities, parts thereof or of chemical compositions
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
    • G01N30/02—Column chromatography
    • G01N2030/022—Column chromatography characterised by the kind of separation mechanism
    • G01N2030/027—Liquid chromatography

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Electrochemistry (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Dispersion Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Theoretical Computer Science (AREA)
  • Computing Systems (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Abstract

La descripción se refiere a métodos para caracterizar composiciones de anfólitos adecuadas para aplicaciones posteriores, tales como el enfoque isoeléctrico capilar mediante cromatografía de líquidos-espectrometría de masas.
MX2022004356A 2019-10-10 2020-10-09 Metodos de cromatografia de liquidos-espectrometria de masas (lc-ms) para analizar la variabilidad entre lotes de anfolitos. MX2022004356A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962913450P 2019-10-10 2019-10-10
PCT/US2020/055031 WO2021072230A1 (en) 2019-10-10 2020-10-09 Liquid chromatography-mass spectrometry (lc-ms) methods for analyzing ampholyte lot variation

Publications (1)

Publication Number Publication Date
MX2022004356A true MX2022004356A (es) 2022-04-27

Family

ID=73172803

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022004356A MX2022004356A (es) 2019-10-10 2020-10-09 Metodos de cromatografia de liquidos-espectrometria de masas (lc-ms) para analizar la variabilidad entre lotes de anfolitos.

Country Status (12)

Country Link
US (3) US12055518B2 (es)
EP (1) EP4042151A1 (es)
JP (2) JP2022552266A (es)
KR (1) KR20220078610A (es)
CN (2) CN114585915B (es)
AU (1) AU2020364006A1 (es)
BR (1) BR112022006749A2 (es)
CA (1) CA3153535A1 (es)
IL (1) IL291806B2 (es)
MX (1) MX2022004356A (es)
MY (1) MY208186A (es)
WO (1) WO2021072230A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2022552266A (ja) 2019-10-10 2022-12-15 リジェネロン・ファーマシューティカルズ・インコーポレイテッド 両性電解質ロットの変動を分析するための液体クロマトグラフィー－質量分析（ｌｃ－ｍｓ）法
EP4413380A4 (en) * 2021-10-08 2025-08-27 Intabio Llc SOFTWARE FOR MICROFLUIDIC SYSTEMS INTERFACING WITH MASS SPECTROMETRY

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5605798A (en) 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
NZ267842A (en) 1993-05-28 1997-09-22 Baylor College Medicine Apparatus for measuring molecular mass (by mass spectrometry) in which the sample is ionised on the sample holder and desorbed therefrom by laser pulses
US5879670A (en) * 1997-03-31 1999-03-09 Calgon Corporation Ampholyte polymers for use in personal care products
GB9717926D0 (en) 1997-08-22 1997-10-29 Micromass Ltd Methods and apparatus for tandem mass spectrometry
US6040575A (en) 1998-01-23 2000-03-21 Analytica Of Branford, Inc. Mass spectrometry from surfaces
JP4129511B2 (ja) * 2002-09-26 2008-08-06 国立大学法人 東京医科歯科大学 高速液体クロマトグラフィーによるアミノ酸の分析方法
WO2007139956A2 (en) * 2006-05-26 2007-12-06 Laboratory Corporation Of America Holdings Methods and systems for the quantitative analysis of biomarkers
WO2013067366A2 (en) * 2011-11-02 2013-05-10 Leco Corporation Ion mobility spectrometer
WO2015051343A1 (en) 2013-10-03 2015-04-09 Northwestern University High throughput mass spectrometric analysis of proteome samples
CN110044998B (zh) 2014-05-12 2021-12-14 奎斯特诊断投资公司 通过质谱法定量他莫昔芬及其代谢物
US20170234852A1 (en) * 2014-09-30 2017-08-17 Bgi Shenzhen Co., Limited Biomarker compositions specific to coronary heart disease patients and uses thereof
US20190064147A1 (en) * 2015-01-18 2019-02-28 Biogen Ma Inc. Formulations and screening of biological therapeutic agents
CN106932459A (zh) * 2015-12-31 2017-07-07 深圳翰宇药业股份有限公司 一种多肽聚合物毛细管等电聚焦分析方法
WO2018064091A1 (en) * 2016-09-27 2018-04-05 Waters Technologies Corporation Multiple attribute monitoring methodologies for complex samples
TWI774827B (zh) * 2017-08-18 2022-08-21 美商雷傑納榮製藥公司 使用影像毛細管等電聚焦以分析樣品基質中蛋白質變異體之方法
SG11202006667VA (en) 2018-01-22 2020-08-28 Univ Pardubice A method of diagnosing cancer based on lipidomic analysis of a body fluid
PT3514545T (pt) 2018-01-22 2021-01-12 Univ Pardubice Um método de diagnóstico de cancro pancreático baseado na análise lipidómica de um fluido corporal
JP2022552266A (ja) 2019-10-10 2022-12-15 リジェネロン・ファーマシューティカルズ・インコーポレイテッド 両性電解質ロットの変動を分析するための液体クロマトグラフィー－質量分析（ｌｃ－ｍｓ）法

Also Published As

Publication number Publication date
IL291806B1 (en) 2025-01-01
US20240345025A1 (en) 2024-10-17
US12313589B2 (en) 2025-05-27
CN114585915B (zh) 2024-02-23
US12055518B2 (en) 2024-08-06
US20210109063A1 (en) 2021-04-15
US20250251362A1 (en) 2025-08-07
JP2025114703A (ja) 2025-08-05
IL291806B2 (en) 2025-05-01
BR112022006749A2 (pt) 2022-06-28
AU2020364006A1 (en) 2022-05-05
KR20220078610A (ko) 2022-06-10
IL291806A (en) 2022-06-01
CA3153535A1 (en) 2021-04-15
MY208186A (en) 2025-04-22
JP2022552266A (ja) 2022-12-15
EP4042151A1 (en) 2022-08-17
CN114585915A (zh) 2022-06-03
AU2020364006A2 (en) 2022-07-21
WO2021072230A1 (en) 2021-04-15
CN118032980A (zh) 2024-05-14

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