MX2020003798A - Metodos para la purificacion de arilsulfatasa a. - Google Patents
Metodos para la purificacion de arilsulfatasa a.Info
- Publication number
- MX2020003798A MX2020003798A MX2020003798A MX2020003798A MX2020003798A MX 2020003798 A MX2020003798 A MX 2020003798A MX 2020003798 A MX2020003798 A MX 2020003798A MX 2020003798 A MX2020003798 A MX 2020003798A MX 2020003798 A MX2020003798 A MX 2020003798A
- Authority
- MX
- Mexico
- Prior art keywords
- asa
- arylsulfatase
- present
- methods
- purification
- Prior art date
Links
- 102100022146 Arylsulfatase A Human genes 0.000 title abstract 5
- 108010036867 Cerebroside-Sulfatase Proteins 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 3
- 238000000746 purification Methods 0.000 title 1
- 239000012620 biological material Substances 0.000 abstract 1
- 239000006143 cell culture medium Substances 0.000 abstract 1
- 238000004587 chromatography analysis Methods 0.000 abstract 1
- 238000011026 diafiltration Methods 0.000 abstract 1
- 238000002641 enzyme replacement therapy Methods 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 238000000108 ultra-filtration Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/43—Enzymes; Proenzymes; Derivatives thereof
- A61K38/46—Hydrolases (3)
- A61K38/465—Hydrolases (3) acting on ester bonds (3.1), e.g. lipases, ribonucleases
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P25/00—Drugs for disorders of the nervous system
- A61P25/28—Drugs for disorders of the nervous system for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/12—Purification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/06—Sulfuric ester hydrolases (3.1.6)
- C12Y301/06001—Arylsulfatase (3.1.6.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/06—Sulfuric ester hydrolases (3.1.6)
- C12Y301/06008—Cerebroside-sulfatase (3.1.6.8)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Neurosurgery (AREA)
- Neurology (AREA)
- Molecular Biology (AREA)
- Sustainable Development (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Epidemiology (AREA)
- Immunology (AREA)
- Gastroenterology & Hepatology (AREA)
- Psychiatry (AREA)
- Hospice & Palliative Care (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
La presente invención proporciona, entre otras cosas, métodos mejorados para purificar la proteína de arilsulfatasa (ASA) producida de forma recombinante para la terapia de reemplazo de enzimas; la presente invención se basa, en parte, en el sorprendente descubrimiento de que la proteína de ASA recombinante se puede purificar a partir de materiales biológicos no procesados, tal como, medio de cultivo celular que contiene ASA, usando un proceso que implica tan pocas como cuatro columnas de cromatografía y únicamente una etapa de ultrafiltración/diafilt ración posterior a la cromatografía.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361750693P | 2013-01-09 | 2013-01-09 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2020003798A true MX2020003798A (es) | 2020-08-03 |
Family
ID=50002885
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2015008875A MX372711B (es) | 2013-01-09 | 2014-01-09 | Metodos para la purificacion de arilsulfatasa a. |
| MX2020003798A MX2020003798A (es) | 2013-01-09 | 2015-07-08 | Metodos para la purificacion de arilsulfatasa a. |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2015008875A MX372711B (es) | 2013-01-09 | 2014-01-09 | Metodos para la purificacion de arilsulfatasa a. |
Country Status (12)
| Country | Link |
|---|---|
| US (3) | US11407984B2 (es) |
| EP (1) | EP2943568B1 (es) |
| JP (5) | JP6427502B2 (es) |
| CN (4) | CN120665836A (es) |
| AU (3) | AU2014205441B2 (es) |
| BR (1) | BR112015015948B1 (es) |
| CA (1) | CA2896979C (es) |
| EA (1) | EA030551B1 (es) |
| ES (1) | ES2768261T3 (es) |
| HK (1) | HK1214622A1 (es) |
| MX (2) | MX372711B (es) |
| WO (1) | WO2014110246A1 (es) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013009686A2 (en) | 2011-07-08 | 2013-01-17 | Shire Human Genetic Therapies, Inc. | Methods for purification of arylsulfatase a |
| WO2014110246A1 (en) | 2013-01-09 | 2014-07-17 | Shire Human Genetic Therapies, Inc. | Methods for purification of arylsulfatase a |
| JP6783767B2 (ja) * | 2014-12-22 | 2020-11-11 | アレクシオン ファーマシューティカルズ インコーポレイテッドＡｌｅｘｉｏｎ Ｐｈａｒｍａｃｅｕｔｉｃａｌｓ， Ｉｎｃ． | 組換えタンパク質を精製する方法 |
| HK1259368A1 (zh) * | 2015-08-20 | 2019-11-29 | F. Hoffmann-La Roche Ag | Fkpa的纯化及其用於生产重组多肽的用途 |
| CN109069592B (zh) | 2016-03-16 | 2023-03-07 | 菲尼克斯组织修复公司 | 纯化胶原7的方法 |
| KR102286260B1 (ko) * | 2017-08-31 | 2021-08-05 | 주식회사 녹십자 | 설파타아제 단백질을 정제하는 방법 |
| BR112020012637A2 (pt) * | 2017-12-19 | 2020-12-01 | Shire Human Genetic Therapies, Inc | arilsulfatase a purificada e composições da mesma |
| KR20200118011A (ko) * | 2018-02-02 | 2020-10-14 | 엔지반트 테라퓨틱스 게엠베하 | 파버병을 치료하는 방법 |
| WO2019199476A1 (en) * | 2018-04-12 | 2019-10-17 | Amgen Inc. | Methods for making stable protein compositions |
| CN112082832B (zh) * | 2019-06-12 | 2022-03-29 | 华南理工大学 | 一种保存城市污水中硫酸酯结合体和葡萄糖苷醛酸结合体的方法 |
| CN110632052A (zh) * | 2019-10-25 | 2019-12-31 | 山西师范大学 | 土壤芳基硫酸酯酶活性的荧光光谱检测法 |
| JP7602542B2 (ja) * | 2019-12-12 | 2024-12-18 | イーライ リリー アンド カンパニー | 環境適合性洗剤によるウイルス不活化のための方法 |
| CN112759643B (zh) * | 2021-03-17 | 2023-05-30 | 华兰生物工程股份有限公司 | 一种血清白蛋白的脱脂方法 |
| CN116693659B (zh) * | 2023-07-11 | 2025-03-28 | 浙江大学 | 一种分离重组人血白蛋白的两步混合模式层析方法 |
Family Cites Families (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4315919A (en) | 1980-10-06 | 1982-02-16 | Edward Shanbrom | Depyrogenation process |
| US4314997A (en) | 1980-10-06 | 1982-02-09 | Edward Shanbrom | Purification of plasma protein products |
| US4764369A (en) | 1983-07-14 | 1988-08-16 | New York Blood Center Inc. | Undenatured virus-free biologically active protein derivatives |
| CA2041280A1 (en) * | 1990-05-10 | 1991-11-11 | Yosuke Sawada | Arylsulfatase |
| NO960163D0 (no) | 1996-01-15 | 1996-01-15 | Thomas Berg | Mutasjonsdeteksjon av bovin |
| PT1049487E (pt) | 1998-01-27 | 2002-10-31 | Hemebiotech As | Tratamento de porfiria aguda interminente (pai) e outras doencas porfiricas |
| PL196770B1 (pl) | 1998-06-09 | 2008-01-31 | Statens Seruminstitut | Sposób oczyszczania immunoglobuliny, produkt immunoglobuliny i jego zastosowanie |
| DK2272870T3 (da) | 1998-06-09 | 2013-08-05 | Csl Behring Ag | Fremgangsmåde til fremstilling af immunoglobuliner med henblik på intravenøs indgivelse og andre immunoglobulin-lignende produkter. |
| AUPQ026799A0 (en) | 1999-05-10 | 1999-06-03 | Csl Limited | Method of increasing protein stability by removing immunoglobulin aggregates |
| WO2001007065A2 (en) | 1999-07-27 | 2001-02-01 | Hemebiotech A/S | PRODUCTION OF rhPBGD AND NEW THERAPEUTIC METHODS FOR TREATING PATIENTS WITH ACUTE INTERMITTENT PORPHYRIA (AIP) AND OTHER PORPHYRIC DISEASES |
| US6812339B1 (en) | 2000-09-08 | 2004-11-02 | Applera Corporation | Polymorphisms in known genes associated with human disease, methods of detection and uses thereof |
| WO2002040686A2 (en) | 2000-11-15 | 2002-05-23 | Genzyme Corporation | Expression system for recombinant proteins |
| WO2002099092A2 (en) | 2001-06-07 | 2002-12-12 | Hemebiotech A/S | Production of recombinant human lysosomal alpha-mannosidase |
| AU2002317700A1 (en) * | 2001-06-07 | 2002-12-16 | Hemebiotech A/S | Production of recombinant human arylsulfatase a |
| WO2003002731A1 (en) | 2001-06-29 | 2003-01-09 | Hemebiotech A/S | A PROCESS FOR PURIFICATION OF RECOMBINANT PORPHOBILINOGEN DEAMINASE (rhPBGD) |
| US7232670B2 (en) | 2001-09-28 | 2007-06-19 | St. Jude Children's Research Hospital | Targeting proteins to cells expressing mannose receptors via expression in insect cells |
| US20050158296A1 (en) | 2002-01-11 | 2005-07-21 | Starr Christopher M. | Use of p97 as an enzyme delivery system for the delivery of therapeutic lysosomal enzymes |
| WO2003066669A2 (en) | 2002-02-04 | 2003-08-14 | Millipore Corporation | Process for removing protein aggregates and virus from a protein solution |
| NZ589037A (en) * | 2004-01-30 | 2011-10-28 | Shire Pharmaceuticals Ireland Ltd | Production and purification of recombinant arylsulfatase A |
| SI1740204T1 (en) | 2004-04-01 | 2018-07-31 | Chiesi Farmaceutici S.P.A. | MEDICAL APPLICATION OF ALFA-MANOSIDES |
| US20060051347A1 (en) | 2004-09-09 | 2006-03-09 | Winter Charles M | Process for concentration of antibodies and therapeutic products thereof |
| PT2628746T (pt) | 2006-04-04 | 2019-04-23 | Chiesi Farm Spa | Um processo para a concentração de um polipéptido |
| CA2739352C (en) * | 2008-10-29 | 2021-07-13 | Wyeth Llc | Methods for purification of single domain antigen binding molecules |
| US8945895B2 (en) * | 2009-07-31 | 2015-02-03 | Baxter International Inc. | Methods of purifying recombinant ADAMTS13 and other proteins and compositions thereof |
| HRP20191923T1 (hr) | 2010-06-25 | 2020-01-10 | Shire Human Genetic Therapies, Inc. | Postupci i sastavi za isporuku arilsulfataze a u cns |
| SMT201600385T1 (it) | 2010-06-25 | 2017-03-08 | Shire Human Genetic Therapies | Trasporto di agenti terapeutici all’snc |
| GB201012603D0 (en) * | 2010-07-27 | 2010-09-08 | Ucb Pharma Sa | Protein purification |
| WO2014110246A1 (en) | 2013-01-09 | 2014-07-17 | Shire Human Genetic Therapies, Inc. | Methods for purification of arylsulfatase a |
-
2014
- 2014-01-09 WO PCT/US2014/010856 patent/WO2014110246A1/en not_active Ceased
- 2014-01-09 CN CN202510851520.1A patent/CN120665836A/zh active Pending
- 2014-01-09 ES ES14701454T patent/ES2768261T3/es active Active
- 2014-01-09 CA CA2896979A patent/CA2896979C/en active Active
- 2014-01-09 MX MX2015008875A patent/MX372711B/es active IP Right Grant
- 2014-01-09 CN CN202510351647.7A patent/CN119979504A/zh active Pending
- 2014-01-09 AU AU2014205441A patent/AU2014205441B2/en active Active
- 2014-01-09 US US14/759,645 patent/US11407984B2/en active Active
- 2014-01-09 CN CN201480003307.5A patent/CN104903442A/zh active Pending
- 2014-01-09 JP JP2015551870A patent/JP6427502B2/ja active Active
- 2014-01-09 CN CN202011058581.6A patent/CN112126634A/zh active Pending
- 2014-01-09 HK HK16102496.7A patent/HK1214622A1/zh unknown
- 2014-01-09 EA EA201590826A patent/EA030551B1/ru not_active IP Right Cessation
- 2014-01-09 BR BR112015015948-6A patent/BR112015015948B1/pt active IP Right Grant
- 2014-01-09 EP EP14701454.2A patent/EP2943568B1/en active Active
-
2015
- 2015-07-08 MX MX2020003798A patent/MX2020003798A/es unknown
-
2018
- 2018-06-28 JP JP2018122837A patent/JP2018171067A/ja not_active Withdrawn
-
2019
- 2019-09-25 JP JP2019174155A patent/JP2020015750A/ja not_active Withdrawn
-
2020
- 2020-02-14 AU AU2020201079A patent/AU2020201079B2/en active Active
-
2021
- 2021-11-10 JP JP2021183082A patent/JP7407161B2/ja active Active
-
2022
- 2022-07-01 US US17/810,396 patent/US11884945B2/en active Active
- 2022-08-25 AU AU2022221475A patent/AU2022221475B2/en active Active
-
2023
- 2023-10-04 JP JP2023173170A patent/JP7663649B2/ja active Active
- 2023-12-22 US US18/394,404 patent/US12319940B2/en active Active
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| MX2020003798A (es) | Metodos para la purificacion de arilsulfatasa a. | |
| PH12020500027A1 (en) | Purification of iduronate-2-sulfatase | |
| MX2020006117A (es) | Integracion dirigida de acidos nucleicos. | |
| NZ743008A (en) | Compositions and methods for internalizing enzymes | |
| HK1206755A1 (en) | Low acidic species compositions and methods for producing and using the same | |
| IN2012DN00801A (es) | ||
| BR112015019879A2 (pt) | Composições de espécies de variantes moduladas de lisina e métodos para produção e uso das mesmas | |
| EA201492185A1 (ru) | Способ получения рекомбинантной идуронат-2-сульфатазы | |
| WO2012031263A3 (en) | Cell culture system for bioreactor scale-up of cells | |
| WO2016022377A3 (en) | Methods for modulating the glycosylation profile of recombinant proteins using dissolved oxygen | |
| WO2015031726A3 (en) | Engineered primate l-methioninase for therapeutic purposes | |
| MX391716B (es) | Promotor de catalasa modificado bidireccional de bacilo. | |
| WO2008085962A3 (en) | Cell culture methods for producing recombinant proteins in the presence of reduced levels of one or more contaminants | |
| WO2014004540A8 (en) | Method for engineering proteases and protein kinases | |
| MX378585B (es) | Sistema de expresion bacterial bicistronico | |
| EA201990593A1 (ru) | Непрерывный способ для снижения гетерогенности терапевтического белка | |
| MY199833A (en) | Method of purifying glycosylated protein from host cell galectins and other contaminants | |
| EA201000945A1 (ru) | Конструирование системы секреции на основе sm-белков | |
| PH12016500060A1 (en) | Expanded bed absorption methods for isolation of basic milk proteins including lactoferrin | |
| UY34915A (es) | Purificación de inhibidor de proteasa alfa1 derivado de cultivo celular | |
| EA201790362A1 (ru) | Солюбилизированный фермент и его применения | |
| EP2067857A4 (en) | PROCESS FOR DIPEPTIDE PRODUCTION | |
| MX2015010887A (es) | Formulaciones y metodos para aumentar la produccion de proteinas recombinantes. | |
| WO2012028753A8 (es) | Procedimiento para la producción y purificación de proteínas recombinantes en plantas | |
| IN2013MU01384A (es) |