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MX2020003362A - MODIFIED STRAINS FOR THE PRODUCTION OF RECOMBINANT SILK. - Google Patents

MODIFIED STRAINS FOR THE PRODUCTION OF RECOMBINANT SILK.

Info

Publication number
MX2020003362A
MX2020003362A MX2020003362A MX2020003362A MX2020003362A MX 2020003362 A MX2020003362 A MX 2020003362A MX 2020003362 A MX2020003362 A MX 2020003362A MX 2020003362 A MX2020003362 A MX 2020003362A MX 2020003362 A MX2020003362 A MX 2020003362A
Authority
MX
Mexico
Prior art keywords
production
modified strains
recombinant silk
strains
modified
Prior art date
Application number
MX2020003362A
Other languages
Spanish (es)
Inventor
Matthew Scott Gamboa
Joshua Tyler Kittleson
Original Assignee
Bolt Threads Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bolt Threads Inc filed Critical Bolt Threads Inc
Publication of MX2020003362A publication Critical patent/MX2020003362A/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/23—Aspartic endopeptidases (3.4.23)
    • C12Y304/23041—Yapsin 1 (3.4.23.41)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/84—Pichia

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Insects & Arthropods (AREA)
  • Biophysics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Botany (AREA)
  • Virology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

En la presente se describen cepas modificadas para reducir la degradación de productos que se expresan de forma recombinante secretados por un organismo hospedador y métodos para el uso de las cepas modificadas.Described herein are strains modified to reduce the degradation of recombinantly expressed products secreted by a host organism and methods for use of the modified strains.

MX2020003362A 2017-10-03 2017-10-03 MODIFIED STRAINS FOR THE PRODUCTION OF RECOMBINANT SILK. MX2020003362A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/US2017/054997 WO2019070246A1 (en) 2017-10-03 2017-10-03 Modified strains for the production of recombinant silk

Publications (1)

Publication Number Publication Date
MX2020003362A true MX2020003362A (en) 2020-07-29

Family

ID=65994932

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2020003362A MX2020003362A (en) 2017-10-03 2017-10-03 MODIFIED STRAINS FOR THE PRODUCTION OF RECOMBINANT SILK.

Country Status (7)

Country Link
EP (1) EP3692054A4 (en)
JP (1) JP7246102B2 (en)
KR (1) KR102558303B1 (en)
CN (2) CN120399914A (en)
AU (3) AU2017434920B2 (en)
MX (1) MX2020003362A (en)
WO (1) WO2019070246A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3794151A4 (en) 2018-05-17 2022-03-23 Bolt Threads, Inc. DRY MODIFIED STRAIN FOR ENHANCED RECOMBINANT PROTEIN SECRETION
WO2023215221A2 (en) * 2022-05-02 2023-11-09 North Carolina State University Engineered microorganisms with enhanced protein expression and secretion

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4464269B2 (en) * 2002-04-26 2010-05-19 協和発酵キリン株式会社 Methylotrophic yeast producing mammalian sugar chains
ATE480624T1 (en) * 2002-06-29 2010-09-15 Korea Res Inst Of Bioscience YAPSIN DEFICIENCY MUTANT STRAIN OF HANSENULA POLYMORPHA AND METHOD FOR PRODUCING RECOMBINANT PROTEINS THEREFROM
AU2003263163A1 (en) * 2002-09-20 2004-04-08 Cym1P A/S Methods for increasing the production of a recombinant polypeptide from a host cell
KR101498668B1 (en) * 2008-12-04 2015-03-06 한국생명공학연구원 Screening of abundantly secreted proteins and their use as fusion partners for the production of recombinant proteins
EP2921551A3 (en) * 2009-02-26 2015-12-02 GlaxoSmithKline LLC Host cells and methods of use
US8440456B2 (en) * 2009-05-22 2013-05-14 Vib, Vzw Nucleic acids of Pichia pastoris and use thereof for recombinant production of proteins
CN104136622A (en) * 2011-12-30 2014-11-05 奥克西雷恩英国有限公司 Methods and materials for reducing degradation of recombinant proteins
CN102676563B (en) * 2012-05-03 2013-09-18 浙江大学 Method for preparing human serum albumin-human parathyroid hormone
EP3046585B1 (en) * 2013-09-17 2021-08-25 Bolt Threads, Inc. Methods and compositions for synthesizing improved silk fibers
CN106701813A (en) * 2016-12-29 2017-05-24 陕西慧康生物科技有限责任公司 Expression vector as well as construction method and application thereof

Also Published As

Publication number Publication date
AU2022202534A1 (en) 2022-05-12
KR102558303B1 (en) 2023-07-21
AU2022202534B2 (en) 2024-09-12
CN111315763B (en) 2025-05-09
CN120399914A (en) 2025-08-01
KR20200058482A (en) 2020-05-27
JP7246102B2 (en) 2023-03-27
CN111315763A (en) 2020-06-19
AU2017434920A1 (en) 2020-05-07
EP3692054A4 (en) 2021-06-09
WO2019070246A1 (en) 2019-04-11
EP3692054A1 (en) 2020-08-12
JP2021503275A (en) 2021-02-12
AU2017434920B2 (en) 2022-02-10
AU2024278323A1 (en) 2025-02-20

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