MX2020001058A - Marcadores de fusión para expresión de proteínas recombinantes. - Google Patents
Marcadores de fusión para expresión de proteínas recombinantes.Info
- Publication number
- MX2020001058A MX2020001058A MX2020001058A MX2020001058A MX2020001058A MX 2020001058 A MX2020001058 A MX 2020001058A MX 2020001058 A MX2020001058 A MX 2020001058A MX 2020001058 A MX2020001058 A MX 2020001058A MX 2020001058 A MX2020001058 A MX 2020001058A
- Authority
- MX
- Mexico
- Prior art keywords
- protein
- fusion
- protein expression
- recombinant protein
- target protein
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Gastroenterology & Hepatology (AREA)
- Toxicology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Diabetes (AREA)
- Endocrinology (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La presente invención se refiere en ciertas modalidades, a una proteína de fusión que comprende un marcador de fusión DEEP y una proteína objetivo (p.ej., proteína de interés). En otras modalidades diferentes, la invención proporciona métodos para producir una proteína de fusión que comprende un marcador de fusión DEEP y una proteína objetivo, constructos que comprenden una secuencia de nucleótidos que codifican un marcador de fusión DEEP y un sitio de clonación para introducir una secuencia de nucleótidos que codifican una proteína objetivo, y kits que comprenden tales constructos de ADN.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201762538505P | 2017-07-28 | 2017-07-28 | |
| PCT/US2018/044156 WO2019023616A1 (en) | 2017-07-28 | 2018-07-27 | FUSION TAGS FOR EXPRESSING RECOMBINANT PROTEINS |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2020001058A true MX2020001058A (es) | 2020-08-06 |
Family
ID=63312460
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2020001058A MX2020001058A (es) | 2017-07-28 | 2018-07-27 | Marcadores de fusión para expresión de proteínas recombinantes. |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US12473340B2 (es) |
| EP (1) | EP3658574A1 (es) |
| JP (1) | JP2020529221A (es) |
| KR (1) | KR20200037819A (es) |
| CN (1) | CN111132996A (es) |
| AU (1) | AU2018307960B2 (es) |
| CA (1) | CA3071419A1 (es) |
| MX (1) | MX2020001058A (es) |
| SG (1) | SG11202000775SA (es) |
| WO (1) | WO2019023616A1 (es) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3658574A1 (en) | 2017-07-28 | 2020-06-03 | The Trustees of Princeton University | Fusion tags for recombinant protein expression |
| JP2022536775A (ja) * | 2019-06-14 | 2022-08-18 | マイタイド・セラピューティクス・インコーポレーテッド | ペプチド及びタンパク質生産のための製造プロセス |
| WO2022094146A1 (en) | 2020-10-28 | 2022-05-05 | The Trustees Of Princeton University | Methods of converting precursor proteins to mature proteins using kex2 proteases |
| CN116981680A (zh) * | 2021-01-14 | 2023-10-31 | 生物催化剂有限责任公司 | 用于肽生产的组合物和方法 |
| CN113388009B (zh) * | 2021-05-18 | 2022-07-19 | 中国科学院福建物质结构研究所 | 一种标签蛋白及其编码基因、重组载体与应用 |
| KR102868986B1 (ko) * | 2022-09-27 | 2025-10-15 | 크리포 주식회사 | 주요 아미노산이 전하성 및 소수성 아미노산으로 구성된 분산된 His-태그 기반 펩타이드 태그를 포함하여 자가 조립체를 형성하는 융합 단백질 및 이를 이용하여 재조합 단백질을 정제하는 방법 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69534185T2 (de) | 1994-06-30 | 2006-02-23 | Novozymes Biotech, Inc., Davis | Nicht-toxisches, nicht-toxigenes, nicht-pathogenes fusarium expressionssystem und darin zu verwendende promotoren und terminatoren |
| WO2008062010A2 (en) | 2006-11-22 | 2008-05-29 | Novo Nordisk A/S | Method for making activated carboxypeptidases |
| DK2831262T3 (en) | 2012-03-29 | 2018-05-28 | Biocon Ltd | SECRETION OF FUNCTIONAL INSULIN GLARGIN DIRECTLY IN THE CULTIVATION MEDIUM THROUGH INTRACELLULAR OVERPRESSION OF KEX2P |
| CN103031328B (zh) * | 2012-11-22 | 2015-08-05 | 江南大学 | 利用一种融合标签提高外源蛋白质的表达量 |
| KR20150131213A (ko) | 2013-03-14 | 2015-11-24 | 인디애나 유니버시티 리서치 앤드 테크놀로지 코퍼레이션 | 인슐린-인크레틴 접합체들 |
| EP3027645A2 (en) | 2013-07-31 | 2016-06-08 | Biogenomics Limited | Process for production of insulin and insulin analogues |
| EP3362468A1 (en) * | 2015-10-13 | 2018-08-22 | The Research Foundation for the State University of New York | Cleavable fusion tag for protein overexpression and purification |
| EP3658574A1 (en) | 2017-07-28 | 2020-06-03 | The Trustees of Princeton University | Fusion tags for recombinant protein expression |
-
2018
- 2018-07-27 EP EP18759004.7A patent/EP3658574A1/en not_active Withdrawn
- 2018-07-27 MX MX2020001058A patent/MX2020001058A/es unknown
- 2018-07-27 KR KR1020207005772A patent/KR20200037819A/ko not_active Withdrawn
- 2018-07-27 CN CN201880062847.9A patent/CN111132996A/zh active Pending
- 2018-07-27 CA CA3071419A patent/CA3071419A1/en active Pending
- 2018-07-27 JP JP2020527858A patent/JP2020529221A/ja active Pending
- 2018-07-27 WO PCT/US2018/044156 patent/WO2019023616A1/en not_active Ceased
- 2018-07-27 SG SG11202000775SA patent/SG11202000775SA/en unknown
- 2018-07-27 US US16/633,991 patent/US12473340B2/en active Active
- 2018-07-27 AU AU2018307960A patent/AU2018307960B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| WO2019023616A1 (en) | 2019-01-31 |
| SG11202000775SA (en) | 2020-02-27 |
| US20200216512A1 (en) | 2020-07-09 |
| AU2018307960B2 (en) | 2023-03-23 |
| JP2020529221A (ja) | 2020-10-08 |
| EP3658574A1 (en) | 2020-06-03 |
| US12473340B2 (en) | 2025-11-18 |
| KR20200037819A (ko) | 2020-04-09 |
| CN111132996A (zh) | 2020-05-08 |
| AU2018307960A1 (en) | 2020-02-27 |
| CA3071419A1 (en) | 2019-01-31 |
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