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MX2019014497A - Metodo para producir celula eucariota de adn editado, y kit usado en el mismo. - Google Patents

Metodo para producir celula eucariota de adn editado, y kit usado en el mismo.

Info

Publication number
MX2019014497A
MX2019014497A MX2019014497A MX2019014497A MX2019014497A MX 2019014497 A MX2019014497 A MX 2019014497A MX 2019014497 A MX2019014497 A MX 2019014497A MX 2019014497 A MX2019014497 A MX 2019014497A MX 2019014497 A MX2019014497 A MX 2019014497A
Authority
MX
Mexico
Prior art keywords
eukaryotic cell
edited
kit used
manufacturing dna
dna
Prior art date
Application number
MX2019014497A
Other languages
English (en)
Inventor
Tomoji Mashimo
Junji Takeda
Hiroyuki MORISAKA
Kazuto Yoshimi
Original Assignee
Univ Osaka
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=64566087&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2019014497(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Univ Osaka filed Critical Univ Osaka
Publication of MX2019014497A publication Critical patent/MX2019014497A/es

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/14—Plant cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Environmental Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Botany (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Public Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

Un sistema CRISPR-Cas3 se estableció de manera exitosa en una célula eucariota.
MX2019014497A 2017-06-08 2018-06-08 Metodo para producir celula eucariota de adn editado, y kit usado en el mismo. MX2019014497A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2017113747 2017-06-08
PCT/JP2018/022066 WO2018225858A1 (ja) 2017-06-08 2018-06-08 Ｄｎａが編集された真核細胞を製造する方法、および当該方法に用いられるキット

Publications (1)

Publication Number Publication Date
MX2019014497A true MX2019014497A (es) 2020-10-12

Family

ID=64566087

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2019014497A MX2019014497A (es) 2017-06-08 2018-06-08 Metodo para producir celula eucariota de adn editado, y kit usado en el mismo.

Country Status (15)

Country Link
US (3) US11807869B2 (es)
EP (2) EP4349973A3 (es)
JP (7) JP6480647B1 (es)
KR (1) KR102541398B1 (es)
CN (2) CN117778466A (es)
AU (1) AU2018279457B2 (es)
BR (1) BR112019025717A2 (es)
CA (1) CA3066599A1 (es)
DK (1) DK3636753T5 (es)
EA (1) EA201992795A1 (es)
ES (1) ES2980050T3 (es)
FI (1) FI3636753T3 (es)
MX (1) MX2019014497A (es)
PT (1) PT3636753T (es)
WO (1) WO2018225858A1 (es)

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JP7017259B2 (ja) * 2017-08-21 2022-02-08 国立大学法人徳島大学 ヌクレオチド標的認識を利用した標的配列特異的改変技術
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WO2020122104A1 (ja) * 2018-12-11 2020-06-18 国立大学法人京都大学 ゲノムｄｎａに欠失を誘導する方法
CN113795587A (zh) * 2019-03-07 2021-12-14 纽约市哥伦比亚大学理事会 使用Tn7样转座子进行RNA引导的DNA整合
JP7489112B2 (ja) * 2019-03-14 2024-05-23 国立大学法人徳島大学 Ｃｒｉｓｐｒタイプｉ－ｄシステムを利用した標的配列改変技術
CN113646429B (zh) 2019-04-05 2025-07-11 国立大学法人大阪大学 敲入细胞的制作方法
KR20220118498A (ko) * 2019-12-18 2022-08-25 인스크립타 인코포레이티드 핵산-가이드된 뉴클레아제 편집된 세포의 생체 내 검출을 위한 캐스케이드/dcas3 상보 검정
JP6940086B1 (ja) * 2020-01-24 2021-09-22 Ｃ４Ｕ株式会社 試料中の特定のｄｎａを検出する方法
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JP7802303B2 (ja) 2021-03-01 2026-01-20 Ｃ４Ｕ株式会社 Ｃａｓ３タンパク質を製造する方法
US20240229081A1 (en) * 2021-05-26 2024-07-11 The Regents Of The University Of Michigan Crispr-cas3 systems for targeted genome engineering
CN113549650B (zh) * 2021-07-05 2023-05-09 天津协和生物科技开发有限公司 一种CRISPR-SaCas9基因编辑系统及其应用
CN115595330B (zh) * 2021-07-12 2024-08-02 中国科学院微生物研究所 一种CRISPR-Cas3系统及其在抗植物病毒方面的应用
JP2023131616A (ja) * 2022-03-09 2023-09-22 国立大学法人広島大学 Ｄｎａ編集システム、並びに、それを用いた標的ｄｎａの編集方法及び標的ｄｎａが編集された細胞の製造方法
CN121195066A (zh) 2023-03-27 2025-12-23 国立大学法人京都大学 具有修饰核苷酸的crRNA
KR20250168544A (ko) 2023-04-26 2025-12-02 유러스 세러퓨틱스 가부시키가이샤 표적 뉴클레오티드 서열의 개변을 위한 비천연형 폴리뉴클레오티드
WO2025023307A1 (ja) * 2023-07-26 2025-01-30 Ｃ４Ｕ株式会社 ガイドｒｎａおよびその利用
JPWO2025094917A1 (es) * 2023-10-30 2025-05-08
WO2025197847A1 (ja) * 2024-03-18 2025-09-25 国立研究開発法人農業・食品産業技術総合研究機構 改良されたＣＲＩＳＰＲ－Ｃａｓ３システム

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JP7017259B2 (ja) * 2017-08-21 2022-02-08 国立大学法人徳島大学 ヌクレオチド標的認識を利用した標的配列特異的改変技術
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JP2024028649A (ja) 2024-03-04
US12371713B2 (en) 2025-07-29
EA201992795A1 (ru) 2020-04-21
JP2019062922A (ja) 2019-04-25
JPWO2018225858A1 (ja) 2019-06-27
PT3636753T (pt) 2024-04-23
WO2018225858A1 (ja) 2018-12-13
FI3636753T3 (fi) 2024-05-03
US20200102580A1 (en) 2020-04-02
EP4349973A2 (en) 2024-04-10
BR112019025717A2 (pt) 2020-09-01
CN110770342B (zh) 2023-11-17
EP4349973A3 (en) 2024-06-26
KR102541398B1 (ko) 2023-06-07
JP2019062921A (ja) 2019-04-25
ES2980050T3 (es) 2024-09-27
JP7430358B2 (ja) 2024-02-13
JP7301332B2 (ja) 2023-07-03
JP7731156B2 (ja) 2025-08-29
KR20200015700A (ko) 2020-02-12
DK3636753T5 (da) 2024-08-26
US20240124898A1 (en) 2024-04-18
JP2025156592A (ja) 2025-10-14
JP2019062923A (ja) 2019-04-25
CN117778466A (zh) 2024-03-29
AU2018279457B2 (en) 2024-02-15
US11807869B2 (en) 2023-11-07
JP2023054185A (ja) 2023-04-13
CN110770342A (zh) 2020-02-07
AU2018279457A1 (en) 2019-12-05
EP3636753B1 (en) 2024-04-03
US20240117381A1 (en) 2024-04-11
EP3636753A4 (en) 2021-03-31
CA3066599A1 (en) 2018-12-13
EP3636753A1 (en) 2020-04-15
JP6480647B1 (ja) 2019-03-13
DK3636753T3 (da) 2024-05-06

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