MX2019001598A - Reduccion de nitrosamina especifica de tabaco en plantas. - Google Patents
Reduccion de nitrosamina especifica de tabaco en plantas.Info
- Publication number
- MX2019001598A MX2019001598A MX2019001598A MX2019001598A MX2019001598A MX 2019001598 A MX2019001598 A MX 2019001598A MX 2019001598 A MX2019001598 A MX 2019001598A MX 2019001598 A MX2019001598 A MX 2019001598A MX 2019001598 A MX2019001598 A MX 2019001598A
- Authority
- MX
- Mexico
- Prior art keywords
- seq
- mutant
- naturally occurring
- plants
- polynucleotide
- Prior art date
Links
- 241000196324 Embryophyta Species 0.000 title 1
- 244000061176 Nicotiana tabacum Species 0.000 title 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 title 1
- XKLJHFLUAHKGGU-UHFFFAOYSA-N nitrous amide Chemical compound ON=N XKLJHFLUAHKGGU-UHFFFAOYSA-N 0.000 title 1
- 102000040430 polynucleotide Human genes 0.000 abstract 4
- 108091033319 polynucleotide Proteins 0.000 abstract 4
- 239000002157 polynucleotide Substances 0.000 abstract 4
- 229920001184 polypeptide Polymers 0.000 abstract 3
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 3
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 3
- 230000009261 transgenic effect Effects 0.000 abstract 3
- 108010062745 Chloride Channels Proteins 0.000 abstract 2
- 102000011045 Chloride Channels Human genes 0.000 abstract 2
- 108091064523 Clc family Proteins 0.000 abstract 2
- 229910002651 NO3 Inorganic materials 0.000 abstract 1
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 239000013598 vector Substances 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
- A24B15/241—Extraction of specific substances
- A24B15/245—Nitrosamines
-
- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B3/00—Preparing tobacco in the factory
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
- C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Virology (AREA)
- Botany (AREA)
- Nutrition Science (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Physiology (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Manufacture Of Tobacco Products (AREA)
Abstract
En un aspecto, se proporciona una célula vegetal de origen no natural o transgénica, mutante, que comprende: (i) un polinucleótido que comprende, consiste de o consiste esencialmente de una secuencia que codifica un miembro de la familia CLC de canales de cloro y que tiene al menos 60% de identidad de secuencia con la SEQ ID NO:1 o SEQ ID NO: 2 o SEQ ID NO: 3 o SEQ ID NO:4 o SEQ ID NO: 10 o SEQ ID NO: 11; (ii) un polipéptido codificado por el polinucleótido establecido en (i); (iii) un polipéptido que comprende, consiste de o consiste esencialmente de una secuencia que codifica un miembro de la familia CLC de canales de cloro y que tiene al menos 60% de identidad de secuencia con SEQ ID NO: 5 o SEQ ID NO: 6, o SEQ ID NO: 7 o SEQ ID NO: 12 o SEQ ID NO: 13 o SEQ ID NO: 14; o (iv) un constructo, vector o vector de expresión que comprende el polinucleótido aislado que se expone en (i); y en donde la expresión o actividad del polinucleótido o el polipéptido es modulada en comparación con una planta de control y en donde los niveles de nitrato en la planta mutante, de origen no natural o transgénica que contiene la célula vegetal mutante, de origen no natural o transgénica se modulan en comparación con la planta de control que contiene la célula vegetal de control.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12198966 | 2012-12-21 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2019001598A true MX2019001598A (es) | 2019-07-01 |
Family
ID=47559184
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2015008084A MX2015008084A (es) | 2012-12-21 | 2013-12-19 | Reduccion de nitrosamina especifica de tabaco en plantas. |
| MX2019001598A MX2019001598A (es) | 2012-12-21 | 2015-06-19 | Reduccion de nitrosamina especifica de tabaco en plantas. |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2015008084A MX2015008084A (es) | 2012-12-21 | 2013-12-19 | Reduccion de nitrosamina especifica de tabaco en plantas. |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US10563215B2 (es) |
| EP (1) | EP2935588A2 (es) |
| JP (1) | JP6693747B2 (es) |
| KR (1) | KR102253223B1 (es) |
| CN (1) | CN105247053B (es) |
| AP (1) | AP2015008563A0 (es) |
| CA (1) | CA2894955C (es) |
| MX (2) | MX2015008084A (es) |
| PH (1) | PH12015501204B1 (es) |
| RU (1) | RU2735254C2 (es) |
| WO (1) | WO2014096283A2 (es) |
Families Citing this family (24)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9980509B2 (en) | 2013-04-05 | 2018-05-29 | R.J. Reynolds Tobacco Company | Modification of bacterial profile of tobacco |
| WO2015197727A2 (en) * | 2014-06-25 | 2015-12-30 | Philip Morris Products S.A | Modulation of nitrate content in plants |
| GB201501941D0 (en) | 2015-02-05 | 2015-03-25 | British American Tobacco Co | Method |
| BR112017023719A2 (pt) * | 2015-05-05 | 2018-07-17 | Univ North Carolina State | planta de tabaco, parte de planta e/ou célula de planta, semente, cultura de planta, produto de tabaco, método para reduzir pon e/ou nnk, método de produção de uma planta e método para produzir um produto de tabaco |
| CN117126815A (zh) * | 2016-03-11 | 2023-11-28 | 奥驰亚客户服务有限公司 | 用于生产烟杈减少或消除的烟草植物和制品的组合物和方法 |
| US20190093117A1 (en) | 2017-07-31 | 2019-03-28 | R. J. Reynolds Tobacco Company | Methods and compositions for viral-based gene editing in plants |
| KR102826087B1 (ko) | 2018-03-28 | 2025-06-27 | 필립모리스 프로덕츠 에스.에이. | 식물 내 환원당 함량 조절 |
| CN111801423A (zh) | 2018-03-28 | 2020-10-20 | 菲利普莫里斯生产公司 | 调节植物中的氨基酸含量 |
| CN113271768B (zh) * | 2018-12-30 | 2024-02-06 | 菲利普莫里斯生产公司 | 经由硝酸盐还原酶的突变来调节植物中的硝酸盐水平 |
| US12344853B2 (en) * | 2019-06-05 | 2025-07-01 | University Of Kentucky Research Foundation | bZIP transcription factors regulate conversion of nicotine to nornicotine and reduce levels of tobacco specific (TSNA) precursors |
| CN110499388B (zh) * | 2019-09-29 | 2023-03-24 | 云南省烟草农业科学研究院 | 鉴别烟草抗斑萎病位点rtsw等位基因类型的共显性标记引物组、鉴别方法及其应用 |
| JP7659547B2 (ja) | 2019-10-01 | 2025-04-09 | フィリップ・モーリス・プロダクツ・ソシエテ・アノニム | 植物における還元糖含有量の調節（ｉｎｖ） |
| JP2022552638A (ja) | 2019-10-01 | 2022-12-19 | フィリップ・モーリス・プロダクツ・ソシエテ・アノニム | 植物における糖およびアミノ酸含有量の調節（ｓｕｌｔｒ３） |
| CN111690661A (zh) * | 2020-06-01 | 2020-09-22 | 云南省烟草农业科学研究院 | 一种烟草NtbHLH13基因突变体及分子鉴定方法和应用 |
| WO2023036691A1 (en) | 2021-09-10 | 2023-03-16 | Philip Morris Products S.A. | Modulating alkaloid profiles in nicotiana tabacum |
| WO2023117661A1 (en) | 2021-12-20 | 2023-06-29 | Philip Morris Products S.A. | Increasing anatabine in tobacco leaf by regulating methyl putrescine oxidase |
| WO2023117701A1 (en) | 2021-12-21 | 2023-06-29 | Philip Morris Products S.A. | Modulation of nicotine production by alteration of nicotinamidase expression or function in plants |
| CN118475234A (zh) | 2021-12-27 | 2024-08-09 | 日本烟草产业株式会社 | 烟草植物 |
| WO2024079137A1 (en) | 2022-10-13 | 2024-04-18 | Philip Morris Products S.A. | Increasing leaf biomass and nitrogen use efficiency by regulating ntp2 |
| EP4658670A1 (en) | 2023-02-02 | 2025-12-10 | Philip Morris Products S.A. | Modulation of genes coding for lysine ketoglutarate reductase |
| CN120530195A (zh) | 2023-02-02 | 2025-08-22 | 菲利普莫里斯生产公司 | 糖转运蛋白的调节 |
| CN117025626B (zh) * | 2023-06-29 | 2025-02-14 | 中国烟草总公司郑州烟草研究院 | 烟草硝酸盐转运蛋白NtNPF7.4及其编码基因、基因编辑载体和应用 |
| CN117025627B (zh) * | 2023-06-29 | 2024-11-01 | 中国烟草总公司郑州烟草研究院 | 烟草氯离子通道蛋白NtCLC13及其编码基因和应用 |
| WO2025003105A1 (en) | 2023-06-29 | 2025-01-02 | Philip Morris Products S.A. | Modulation of genes coding for glutamate dehydrogenase |
Family Cites Families (19)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5810020A (en) | 1993-09-07 | 1998-09-22 | Osmotek, Inc. | Process for removing nitrogen-containing anions and tobacco-specific nitrosamines from tobacco products |
| US6202649B1 (en) | 1996-12-02 | 2001-03-20 | Regent Court Technologies | Method of treating tobacco to reduce nitrosamine content, and products produced thereby |
| US6586661B1 (en) * | 1997-06-12 | 2003-07-01 | North Carolina State University | Regulation of quinolate phosphoribosyl transferase expression by transformation with a tobacco quinolate phosphoribosyl transferase nucleic acid |
| GEP20033111B (en) | 1997-06-20 | 2003-11-25 | Regent Court Tech | Method of Preventing Nitrosamine Formation in Tobacco Product |
| US7700834B2 (en) * | 2001-11-13 | 2010-04-20 | U.S. Smokless Tobacco Company | Nicotiana nucleic acid molecules and uses thereof |
| RU2191529C1 (ru) | 2001-12-21 | 2002-10-27 | Романенко Александр Павлович | Устройство для курения |
| EP1623634B1 (en) * | 2003-05-06 | 2013-06-12 | Japan Tobacco Inc. | Process for producing regenerated tobacco material |
| JP4160868B2 (ja) | 2003-07-10 | 2008-10-08 | 日本たばこ産業株式会社 | 葉タバコ中のニトロソアミン含量の低減方法 |
| KR20060128821A (ko) * | 2003-08-19 | 2006-12-14 | 22엔디 센츄리 리미티드, 엘엘씨 | 감소된 노출 담배 제품 |
| WO2006091194A1 (en) | 2005-02-23 | 2006-08-31 | North Carolina State University | Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes |
| CA2666383C (en) | 2006-10-13 | 2015-12-22 | North Carolina State University | Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes |
| RU2324737C1 (ru) * | 2006-10-18 | 2008-05-20 | Институт цитологии и генетики Сибирского отделения Российской академии наук (СО РАН) | Способ получения трансгенных растений табака с повышенным содержанием пролина |
| ES2716864T3 (es) | 2007-06-06 | 2019-06-17 | Monsanto Technology Llc | Genes y usos para la mejora de plantas |
| US8124851B2 (en) | 2007-11-12 | 2012-02-28 | North Carolina State University | Alteration of tobacco alkaloid content through modification of specific cytochrome P450 genes |
| CN104131030A (zh) | 2007-12-13 | 2014-11-05 | 菲利普莫里斯生产公司 | 就减少的镉转运进行修饰的转基因植物、衍生产物和相关方法 |
| US20120017338A1 (en) | 2008-01-15 | 2012-01-19 | Wei Wu | Isolated novel nucleic acid and protein molecules from corn and methods of using those molecules to generate transgenic plant with enhanced agronomic traits |
| CA2777802A1 (en) * | 2009-10-22 | 2011-04-28 | Basf Plant Science Company Gmbh | Plants having enhanced yield-related traits and a method for making the same |
| WO2011088180A1 (en) | 2010-01-15 | 2011-07-21 | North Carolina State University | Compositions and methods for minimizing nornicotine synthesis in tobacco |
| AU2011297946B2 (en) | 2010-09-03 | 2015-08-20 | Philip Morris Products S.A. | Heavy metal reduction in planta |
-
2013
- 2013-12-19 US US14/653,924 patent/US10563215B2/en active Active
- 2013-12-19 WO PCT/EP2013/077532 patent/WO2014096283A2/en not_active Ceased
- 2013-12-19 CN CN201380073174.4A patent/CN105247053B/zh active Active
- 2013-12-19 AP AP2015008563A patent/AP2015008563A0/xx unknown
- 2013-12-19 KR KR1020157018188A patent/KR102253223B1/ko active Active
- 2013-12-19 PH PH1/2015/501204A patent/PH12015501204B1/en unknown
- 2013-12-19 MX MX2015008084A patent/MX2015008084A/es unknown
- 2013-12-19 JP JP2015548608A patent/JP6693747B2/ja active Active
- 2013-12-19 EP EP13817681.3A patent/EP2935588A2/en active Pending
- 2013-12-19 RU RU2015126909A patent/RU2735254C2/ru active
- 2013-12-19 CA CA2894955A patent/CA2894955C/en active Active
-
2015
- 2015-06-19 MX MX2019001598A patent/MX2019001598A/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| PH12015501204A1 (en) | 2015-08-17 |
| CN105247053A (zh) | 2016-01-13 |
| WO2014096283A2 (en) | 2014-06-26 |
| US10563215B2 (en) | 2020-02-18 |
| KR20150096451A (ko) | 2015-08-24 |
| KR102253223B1 (ko) | 2021-05-21 |
| RU2015126909A (ru) | 2017-01-27 |
| CA2894955A1 (en) | 2014-06-26 |
| JP6693747B2 (ja) | 2020-05-13 |
| CN105247053B (zh) | 2020-07-31 |
| RU2735254C2 (ru) | 2020-10-29 |
| WO2014096283A3 (en) | 2014-08-14 |
| EP2935588A2 (en) | 2015-10-28 |
| JP2016508032A (ja) | 2016-03-17 |
| PH12015501204B1 (en) | 2022-07-15 |
| CA2894955C (en) | 2023-10-31 |
| MX2015008084A (es) | 2016-03-31 |
| US20150315603A1 (en) | 2015-11-05 |
| AP2015008563A0 (en) | 2015-06-30 |
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