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MX2018015654A - Cromatografía de interacción hidrofóbica para la purificación de oligonucleótidos. - Google Patents

Cromatografía de interacción hidrofóbica para la purificación de oligonucleótidos.

Info

Publication number
MX2018015654A
MX2018015654A MX2018015654A MX2018015654A MX2018015654A MX 2018015654 A MX2018015654 A MX 2018015654A MX 2018015654 A MX2018015654 A MX 2018015654A MX 2018015654 A MX2018015654 A MX 2018015654A MX 2018015654 A MX2018015654 A MX 2018015654A
Authority
MX
Mexico
Prior art keywords
oligonucleotides
purification
hydrophobic interaction
interaction chromatography
chromatography
Prior art date
Application number
MX2018015654A
Other languages
English (en)
Inventor
S Gronke Robert
S Joshi Ratnesh
Original Assignee
Biogen Ma Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biogen Ma Inc filed Critical Biogen Ma Inc
Publication of MX2018015654A publication Critical patent/MX2018015654A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/30—Partition chromatography
    • B01D15/305—Hydrophilic interaction chromatography [HILIC]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/32—Bonded phase chromatography
    • B01D15/325—Reversed phase
    • B01D15/327—Reversed phase with hydrophobic interaction
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
    • B01D15/424—Elution mode
    • B01D15/426—Specific type of solvent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)

Abstract

La presente invención se dirige a un método de purificación de oligonucleótidos mediante cromatografía de interacción hidrofóbica.
MX2018015654A 2016-06-14 2017-06-13 Cromatografía de interacción hidrofóbica para la purificación de oligonucleótidos. MX2018015654A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662349970P 2016-06-14 2016-06-14
US201762492402P 2017-05-01 2017-05-01
PCT/US2017/037126 WO2017218454A1 (en) 2016-06-14 2017-06-13 Hydrophobic interaction chromatography for purification of oligonucleotides

Publications (1)

Publication Number Publication Date
MX2018015654A true MX2018015654A (es) 2019-06-10

Family

ID=59216016

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2018015654A MX2018015654A (es) 2016-06-14 2017-06-13 Cromatografía de interacción hidrofóbica para la purificación de oligonucleótidos.

Country Status (12)

Country Link
US (3) US10947262B2 (es)
EP (1) EP3468981B1 (es)
JP (1) JP7075359B2 (es)
KR (1) KR102461234B1 (es)
CN (1) CN109641928B (es)
AU (1) AU2017284202B2 (es)
BR (1) BR112018075667B1 (es)
ES (1) ES2988362T3 (es)
IL (1) IL263639B (es)
MX (1) MX2018015654A (es)
NZ (1) NZ749360A (es)
WO (1) WO2017218454A1 (es)

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WO2016040748A1 (en) 2014-09-12 2016-03-17 Ionis Pharmaceuticals, Inc. Compositions and methods for detection of smn protein in a subject and treatment of a subject
AR102198A1 (es) 2014-10-09 2017-02-08 Regeneron Pharma Proceso para reducir partículas subvisibles en una formulación farmacéutica
CN119119244A (zh) 2016-08-16 2024-12-13 里珍纳龙药品有限公司 用于对混合物中的个体抗体进行量化的方法
KR102564393B1 (ko) 2016-10-25 2023-08-08 리제너론 파아마슈티컬스, 인크. 크로마토그래피 데이터 분석을 위한 방법 및 시스템
IL272954B2 (en) 2017-09-19 2024-06-01 Regeneron Pharma Methods of reducing particle formation and compositions formed thereby
TWI853823B (zh) 2018-07-02 2024-09-01 美商里珍納龍藥品有限公司 自混合物製備多肽之系統及方法
WO2020072062A1 (en) * 2018-10-04 2020-04-09 Gsw Creative Corporation Portable charging case
CN110066789B (zh) * 2019-05-17 2021-04-20 通用生物系统（安徽）有限公司 一种长链dna引物的hplc纯化改进的方法
EP3980073A4 (en) * 2019-06-07 2023-11-01 Dyne Therapeutics, Inc. METHOD FOR PRODUCING PROTEIN-OLIGONUCLEOTIDE COMPLEXES
CA3173034A1 (en) 2020-02-28 2021-09-02 Ionis Pharmaceuticals, Inc. Compounds and methods for modulating smn2
WO2022187312A1 (en) * 2021-03-02 2022-09-09 Ionis Pharmaceuticals, Inc. Purification methods for oligomeric compounds
EP4585604A1 (en) 2022-09-07 2025-07-16 Nippon Shokubai Co., Ltd. Nucleic acid production method, method for removing impurities, and nucleic acid having less impurities
CN117050120A (zh) * 2022-12-09 2023-11-14 苏州赛分科技股份有限公司 一种纯化小干扰rna的柱层析法
WO2024175051A1 (zh) * 2023-02-23 2024-08-29 奥安医药（苏州）有限公司 一种疏水层析制备寡核苷酸的方法
WO2025149349A1 (en) 2024-01-09 2025-07-17 Cytiva Bioprocess R&D Ab Method for separating nucleic acid molecules

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JPS60104097A (ja) * 1983-11-08 1985-06-08 Sagami Chem Res Center Ｄνａの精製方法
DE3433649A1 (de) * 1984-09-13 1986-03-20 Gesellschaft für Biotechnologische Forschung mbH (GBF), 3300 Braunschweig Verfahren zur aufreinigung synthetischer oligonucleotide
US6087491A (en) * 1993-01-08 2000-07-11 Hybridon, Inc. Extremely high purity oligonucleotides and methods of synthesizing them using dimer blocks
WO1996001268A1 (en) * 1994-07-05 1996-01-18 Hybridon, Inc. Purification of oligodeoxynucleotide phosphorothioates using deae 5pw anion ion-exchange chromatography and hydrophobic interaction chromatography
CN1155887A (zh) * 1994-07-05 1997-07-30 海布里顿公司 用deae-5pw阴离子-离子交换色谱和疏水作用色谱纯化硫代磷酸寡脱氧核苷酸
EP0979233A4 (en) * 1997-04-21 2001-03-21 Proligo L L C Method for solution phase synthesis of oligonucleotides
JP2000035423A (ja) 1998-05-11 2000-02-02 Tosoh Corp 液体クロマトグラフィ―による核酸の分離法
ATE433456T1 (de) * 1999-05-28 2009-06-15 Lonza Biolog Inc Methoden zur reinigung von dns
PT102491B (pt) 2000-07-10 2003-02-28 Inst Superior Tecnico Processo para producao e purificacao de dna plasmidico
US7345163B2 (en) 2002-08-28 2008-03-18 Quiatech Ab Process for separating and deprotecting oligonucleotides
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Also Published As

Publication number Publication date
CA3027288A1 (en) 2017-12-21
US20250011357A1 (en) 2025-01-09
US10947262B2 (en) 2021-03-16
US20210269467A1 (en) 2021-09-02
NZ749360A (en) 2025-11-28
IL263639B (en) 2021-09-30
CN109641928B (zh) 2022-08-30
KR102461234B1 (ko) 2022-10-28
AU2017284202A1 (en) 2019-01-17
WO2017218454A1 (en) 2017-12-21
ES2988362T3 (es) 2024-11-20
KR20190018467A (ko) 2019-02-22
BR112018075667B1 (pt) 2024-02-20
BR112018075667A2 (pt) 2019-04-09
EP3468981A1 (en) 2019-04-17
IL263639A (en) 2019-01-31
EP3468981B1 (en) 2024-07-24
US20190248823A1 (en) 2019-08-15
CN109641928A (zh) 2019-04-16
JP7075359B2 (ja) 2022-05-25
AU2017284202B2 (en) 2021-11-04
JP2019518759A (ja) 2019-07-04

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