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MX2018013175A - Metodo de purificacion para celulas precursoras pancreaticas derivadas a partir de celulas madre pluripotentes y metodo de amplificacion para ellas. - Google Patents

Metodo de purificacion para celulas precursoras pancreaticas derivadas a partir de celulas madre pluripotentes y metodo de amplificacion para ellas.

Info

Publication number
MX2018013175A
MX2018013175A MX2018013175A MX2018013175A MX2018013175A MX 2018013175 A MX2018013175 A MX 2018013175A MX 2018013175 A MX2018013175 A MX 2018013175A MX 2018013175 A MX2018013175 A MX 2018013175A MX 2018013175 A MX2018013175 A MX 2018013175A
Authority
MX
Mexico
Prior art keywords
pancreatic progenitor
cells
progenitor cells
cells derived
pluripotent stem
Prior art date
Application number
MX2018013175A
Other languages
English (en)
Inventor
Iwata Hiroo
Konagaya Shuhei
Original Assignee
Takeda Pharmaceuticals Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takeda Pharmaceuticals Co filed Critical Takeda Pharmaceuticals Co
Publication of MX2018013175A publication Critical patent/MX2018013175A/es

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    • C—CHEMISTRY; METALLURGY
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    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C12N5/0677—Three-dimensional culture, tissue culture or organ culture; Encapsulated cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C12N5/0678—Stem cells; Progenitor cells; Precursor cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00—Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10—Preservation of living parts
    • A01N1/12—Chemical aspects of preservation
    • A01N1/122—Preservation or perfusion media
    • A01N1/125—Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0062—General methods for three-dimensional culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/113—Acidic fibroblast growth factor (aFGF, FGF-1)
    • C—CHEMISTRY; METALLURGY
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/117—Keratinocyte growth factors (KGF-1, i.e. FGF-7; KGF-2, i.e. FGF-12)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/119—Other fibroblast growth factors, e.g. FGF-4, FGF-8, FGF-10
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Se describen un método para cultivar células progenitoras pancreáticas derivadas a partir de células madre pluripotentes, el método comprende el paso (a) de cultivar tridimensionalmente células progenitoras pancreáticas derivadas a partir de células madre pluripotentes en un medio que contiene un factor perteneciente a la familia de factor de crecimiento epidérmico (EGF) y/o un factor perteneciente a la familia de factor de crecimiento de fibroblastos (FGF) y (2) un agonista de Wnt; un método para producir células de islote a partir de células progenitoras pancreáticas derivadas a partir de células madre pluripotentes, el método comprende el paso (E) de inducir la diferenciación de células progenitoras pancreáticas cultivadas mediante el método anterior en células de islote; y un método para crioconservar células progenitoras pancreáticas derivadas a partir de células madre pluripotentes, el método que comprende el paso (F) de congelar células progenitoras pancreáticas cultivadas por el método anterior.
MX2018013175A 2016-04-28 2017-04-27 Metodo de purificacion para celulas precursoras pancreaticas derivadas a partir de celulas madre pluripotentes y metodo de amplificacion para ellas. MX2018013175A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2016091116 2016-04-28
PCT/JP2017/016728 WO2017188378A1 (ja) 2016-04-28 2017-04-27 多能性幹細胞由来膵前駆細胞の純化法とその増幅法

Publications (1)

Publication Number Publication Date
MX2018013175A true MX2018013175A (es) 2019-02-21

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MX2018013175A MX2018013175A (es) 2016-04-28 2017-04-27 Metodo de purificacion para celulas precursoras pancreaticas derivadas a partir de celulas madre pluripotentes y metodo de amplificacion para ellas.

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Country Link
US (1) US20190136197A1 (es)
EP (1) EP3450541A4 (es)
JP (1) JP7055740B2 (es)
KR (1) KR102257868B1 (es)
CN (1) CN109415689A (es)
AU (1) AU2017255099B2 (es)
CA (1) CA3022365A1 (es)
EA (1) EA201892447A1 (es)
IL (1) IL262554A (es)
MX (1) MX2018013175A (es)
SG (1) SG11201809400UA (es)
TW (1) TWI710636B (es)
WO (1) WO2017188378A1 (es)

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EP3833365A4 (en) 2018-08-10 2022-05-11 Vertex Pharmaceuticals Incorporated Stem cell derived islet differentiation
CN112888778B (zh) * 2018-10-15 2025-06-03 埃维亚生命科学有限公司 通过低分子化合物由源自内胚层组织或器官的细胞制备干细胞/祖细胞的方法
CN109749986B (zh) * 2019-03-13 2021-04-02 武汉大学 一种由人多能干细胞分化获得胰腺前体细胞及胰岛β细胞的方法
JP6712740B1 (ja) * 2019-03-25 2020-06-24 学校法人東海大学 Ｔｉｅ２陽性幹／前駆細胞を含む細胞集団の培養方法およびその利用
KR20210145178A (ko) * 2019-04-01 2021-12-01 스템사이트 오와이 Abcg2-양성 각막 주변부 줄기 세포를 수득 또는 유지하는 방법
MX2022008618A (es) 2020-01-21 2022-08-08 Takeda Pharmaceuticals Co Composiciones y metodos de celulas de criopreservacion.
CN112961823B (zh) * 2021-03-19 2024-01-23 上海爱萨尔生物科技有限公司 一种诱导多能干细胞定向分化制备胰岛β细胞的培养液
CN112980774B (zh) * 2021-03-19 2022-04-01 上海爱萨尔生物科技有限公司 一种诱导多能干细胞定向分化制备胰岛β细胞的培养方法
CN113046299B (zh) * 2021-03-19 2024-06-28 上海爱萨尔生物科技有限公司 一种诱导多能干细胞定向分化制备胰岛β细胞的添加剂
CN113234664B (zh) * 2021-05-11 2024-05-10 澳门大学 一种胰腺祖细胞的制备方法及其应用
CN115011544B (zh) * 2022-05-30 2023-11-17 广州国家实验室 体外诱导获得胰岛δ细胞的方法及其应用
CN120330136B (zh) * 2025-06-13 2025-10-03 南京兰伯艾克斯生物科技有限公司 一种干细胞克隆培养方法及无血清培养基

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JP6161603B2 (ja) * 2012-05-25 2017-07-12 学校法人 埼玉医科大学 膵臓ホルモン産生細胞の生産方法及び膵臓ホルモン産生細胞、並びに分化誘導促進剤
JP2014161257A (ja) * 2013-02-22 2014-09-08 Univ Of Tokyo 多能性幹細胞から膵ランゲルハンス島を製造する方法
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Also Published As

Publication number Publication date
CN109415689A (zh) 2019-03-01
IL262554A (en) 2018-12-31
US20190136197A1 (en) 2019-05-09
AU2017255099B2 (en) 2021-07-15
TW201800576A (zh) 2018-01-01
KR102257868B1 (ko) 2021-05-27
EP3450541A1 (en) 2019-03-06
EA201892447A1 (ru) 2019-04-30
AU2017255099A1 (en) 2018-11-22
EP3450541A4 (en) 2019-11-13
TWI710636B (zh) 2020-11-21
CA3022365A1 (en) 2017-11-02
JPWO2017188378A1 (ja) 2019-04-04
SG11201809400UA (en) 2018-11-29
WO2017188378A1 (ja) 2017-11-02
KR20180136544A (ko) 2018-12-24
JP7055740B2 (ja) 2022-04-18

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