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MX2018009493A - Uso combinado de al menos una endoproteasa y al menos una exoproteasa en un proceso simultaneo de sacarificacion y fermentacion (ssf) para mejorar la produccion de etanol. - Google Patents

Uso combinado de al menos una endoproteasa y al menos una exoproteasa en un proceso simultaneo de sacarificacion y fermentacion (ssf) para mejorar la produccion de etanol.

Info

Publication number
MX2018009493A
MX2018009493A MX2018009493A MX2018009493A MX2018009493A MX 2018009493 A MX2018009493 A MX 2018009493A MX 2018009493 A MX2018009493 A MX 2018009493A MX 2018009493 A MX2018009493 A MX 2018009493A MX 2018009493 A MX2018009493 A MX 2018009493A
Authority
MX
Mexico
Prior art keywords
protease
exo
endo
combined use
ssf process
Prior art date
Application number
MX2018009493A
Other languages
English (en)
Inventor
Fukuyama Shiro
Ayabe Keiichi
Matsui Tomoko
Leong SOONG Chee-
Peng Wei
Li Ming
Guo Xinyan
Vidal Bernardo Jr
Jensen Kenneth
P Lessard Louis
E Kreel Nathaniel
Tang Lan
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Publication of MX2018009493A publication Critical patent/MX2018009493A/es

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/485Exopeptidases (3.4.11-3.4.19)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/04Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

La presente invención se refiere a procesos mejorados para producir etanol a partir de materiales que contienen almidón mediante el uso combinado de al menos una endoproteasa y al menos una exoproteasa en un proceso SSF. Más particularmente, la exoproteasa debe constituir al menos un 5% (p/p) de la mezcla de proteasa.
MX2018009493A 2016-03-01 2017-03-01 Uso combinado de al menos una endoproteasa y al menos una exoproteasa en un proceso simultaneo de sacarificacion y fermentacion (ssf) para mejorar la produccion de etanol. MX2018009493A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662301848P 2016-03-01 2016-03-01
CN2016089605 2016-07-11
PCT/CN2017/075326 WO2017148389A1 (en) 2016-03-01 2017-03-01 Combined use of at least one endo-protease and at least one exo-protease in an ssf process for improving ethanol yield

Publications (1)

Publication Number Publication Date
MX2018009493A true MX2018009493A (es) 2018-09-05

Family

ID=59742566

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2018009493A MX2018009493A (es) 2016-03-01 2017-03-01 Uso combinado de al menos una endoproteasa y al menos una exoproteasa en un proceso simultaneo de sacarificacion y fermentacion (ssf) para mejorar la produccion de etanol.

Country Status (6)

Country Link
US (1) US20200165591A1 (es)
EP (1) EP3423584A4 (es)
CN (1) CN108884478A (es)
CA (1) CA3012508A1 (es)
MX (1) MX2018009493A (es)
WO (1) WO2017148389A1 (es)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3630989A1 (en) * 2017-06-02 2020-04-08 Novozymes A/S Improved yeast for ethanol production
WO2019046232A1 (en) * 2017-08-30 2019-03-07 Novozymes A/S Combined use of an endo-protease of the m35 family and an exo-protease of the s53 family in the fermentation of starch
CN111164214A (zh) 2017-09-15 2020-05-15 诺维信公司 用于改进动物饲料营养质量的酶共混物和方法
CN113302294A (zh) * 2019-01-31 2021-08-24 诺维信公司 具有木聚糖酶活性的多肽及其用于改善动物饲料营养质量的用途
BR112021026477A2 (pt) * 2019-08-06 2022-02-08 Novozymes As Célula hospedeira recombinante, métodos de produção de um produto de fermentação a partir de um material contendo amido ou contendo celulose, de produção do polipeptídeo maduro, de produção de um derivado de uma célula hospedeira recombinante e de produção de etanol, construto de ácido nucleico ou vetor de expressão, composição, e, uso de uma célula hospedeira recombinante
CN112680371A (zh) * 2019-10-18 2021-04-20 广东利世康低碳科技有限公司 一种水解餐厨垃圾中蛋白成分的产朊假丝酵母双基因共表达菌株及其构建方法
BR112022013472A2 (pt) * 2020-01-07 2022-09-13 Danisco Us Inc Métodos para produzir etanol e composição de protease
EP4638768A2 (en) 2022-12-19 2025-10-29 Novozymes A/S Processes for producing fermentation products using fiber-degrading enzymes with engineered yeast
WO2024137252A1 (en) 2022-12-19 2024-06-27 Novozymes A/S Process for reducing syrup viscosity in the backend of a process for producing a fermentation product
CN120693401A (zh) 2022-12-19 2025-09-23 诺维信公司 包含阿拉伯呋喃糖苷酶和木聚糖酶的组合物及其用于增加半纤维素纤维溶解的用途
EP4638725A1 (en) 2022-12-19 2025-10-29 Novozymes A/S Carbohydrate esterase family 3 (ce3) polypeptides having acetyl xylan esterase activity and polynucleotides encoding same
CN120380140A (zh) 2022-12-19 2025-07-25 诺维信公司 具有阿魏酸酯酶和/或乙酰木聚糖酯酶活性的碳水化合物酯酶家族1(ce1)多肽和编码其的多核苷酸
AU2024303961A1 (en) 2023-06-13 2025-12-11 Novozymes A/S Processes for producing fermentation products using engineered yeast expressing a beta-xylosidase
WO2025128568A1 (en) 2023-12-11 2025-06-19 Novozymes A/S Composition and use thereof for increasing hemicellulosic fiber solubilization
WO2026008449A2 (en) 2024-07-04 2026-01-08 Novozymes A/S A process for producing a fermentation product and a concentrated protein co-product
CN119823971A (zh) * 2025-01-20 2025-04-15 江南大学 米曲霉来源蛋白酶及其在豆类发酵产品中的应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101517072A (zh) * 2004-01-16 2009-08-26 诺维信北美公司 生产发酵产物的方法
US7820419B2 (en) * 2005-02-07 2010-10-26 Novozymes North America Inc. Fermentation product production processes
CN101959425A (zh) * 2008-03-05 2011-01-26 诺维信公司 饲料产品中黄曲霉毒素的解毒
CN102083991A (zh) * 2008-06-23 2011-06-01 诺维信公司 生产发酵产物的方法
CN103987850B (zh) * 2011-10-11 2021-10-22 诺维信北美公司 用于产生发酵产物的方法
ES2935920T3 (es) * 2012-03-30 2023-03-13 Novozymes North America Inc Procesos de elaboración de productos de fermentación
US10407698B2 (en) * 2013-06-20 2019-09-10 Novozymes A/S Fermentation processes with reduced foaming
EP3074426B1 (en) * 2013-11-26 2020-07-08 Novozymes A/S Milling process
US20170145443A1 (en) * 2014-03-21 2017-05-25 Novozymes A/S Processes Of Producing Ethanol Using A Fermentation Organism

Also Published As

Publication number Publication date
US20200165591A1 (en) 2020-05-28
EP3423584A4 (en) 2020-02-26
EP3423584A1 (en) 2019-01-09
CN108884478A (zh) 2018-11-23
WO2017148389A1 (en) 2017-09-08
CA3012508A1 (en) 2017-09-08

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