MX2018008464A - Metodos para separar isoformas de anticuerpos monoclonales. - Google Patents
Metodos para separar isoformas de anticuerpos monoclonales.Info
- Publication number
- MX2018008464A MX2018008464A MX2018008464A MX2018008464A MX2018008464A MX 2018008464 A MX2018008464 A MX 2018008464A MX 2018008464 A MX2018008464 A MX 2018008464A MX 2018008464 A MX2018008464 A MX 2018008464A MX 2018008464 A MX2018008464 A MX 2018008464A
- Authority
- MX
- Mexico
- Prior art keywords
- antibody
- charge variants
- preparation
- methods
- monoclonal antibodies
- Prior art date
Links
- 102000001708 Protein Isoforms Human genes 0.000 title 1
- 108010029485 Protein Isoforms Proteins 0.000 title 1
- 238000002360 preparation method Methods 0.000 abstract 3
- 229960000106 biosimilars Drugs 0.000 abstract 1
- 238000005341 cation exchange Methods 0.000 abstract 1
- 238000010828 elution Methods 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/18—Ion-exchange chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
- C07K2317/41—Glycosylation, sialylation, or fucosylation
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Oncology (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
Las variantes de carga de una población de anticuerpos expresado de forma recombinante se pueden separar tanto de la molécula del anticuerpo principal como una de la otra. La separación y el aislamiento de las variantes de carga pueden proceder a través de una modulación combinada de concentración de sal y pH durante la elución de la variante de carga a partir de un soporte de intercambio catiónico. Las variantes de carga aisladas pueden ser evaluadas por su contribución a la potencia de la preparación general de anticuerpo. La composición de una preparación de anticuerpos, al menos en términos de la proporción de las variantes de carga y del anticuerpo principal puede así ser controlada, por ejemplo, para una coincidencia biosimilar o para mejorar la potencia de la preparación.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662276378P | 2016-01-08 | 2016-01-08 | |
| PCT/US2017/012477 WO2017120435A1 (en) | 2016-01-08 | 2017-01-06 | Methods for separating isoforms of monoclonal antibodies |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2018008464A true MX2018008464A (es) | 2019-05-30 |
Family
ID=58010365
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2018008464A MX2018008464A (es) | 2016-01-08 | 2017-01-06 | Metodos para separar isoformas de anticuerpos monoclonales. |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20190016753A1 (es) |
| EP (1) | EP3400247A1 (es) |
| JP (1) | JP2019504060A (es) |
| CN (1) | CN109641969A (es) |
| AU (1) | AU2017205477A1 (es) |
| CA (1) | CA3010612A1 (es) |
| MX (1) | MX2018008464A (es) |
| WO (1) | WO2017120435A1 (es) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10704021B2 (en) | 2012-03-15 | 2020-07-07 | Flodesign Sonics, Inc. | Acoustic perfusion devices |
| WO2015105955A1 (en) | 2014-01-08 | 2015-07-16 | Flodesign Sonics, Inc. | Acoustophoresis device with dual acoustophoretic chamber |
| US11377651B2 (en) | 2016-10-19 | 2022-07-05 | Flodesign Sonics, Inc. | Cell therapy processes utilizing acoustophoresis |
| US11708572B2 (en) | 2015-04-29 | 2023-07-25 | Flodesign Sonics, Inc. | Acoustic cell separation techniques and processes |
| US11214789B2 (en) | 2016-05-03 | 2022-01-04 | Flodesign Sonics, Inc. | Concentration and washing of particles with acoustics |
| CN108101987A (zh) * | 2017-11-17 | 2018-06-01 | 安徽未名生物医药有限公司 | 一种重组抗TNF-α全人源单克隆抗体的纯化方法 |
| SG11202003907WA (en) | 2017-12-14 | 2020-05-28 | Flodesign Sonics Inc | Acoustic transducer drive and controller |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009017491A1 (en) * | 2006-06-14 | 2009-02-05 | Smithkline Beecham Corporation | Methods for purifying antibodies using ceramic hydroxyapatite |
| EP2236604B1 (en) * | 2007-12-05 | 2016-07-06 | Chugai Seiyaku Kabushiki Kaisha | Anti-nr10 antibody and use thereof |
| TWI472339B (zh) * | 2008-01-30 | 2015-02-11 | Genentech Inc | 包含結合至ｈｅｒ２結構域ii之抗體及其酸性變異體的組合物 |
| MX357821B (es) * | 2010-12-21 | 2018-07-25 | Hoffmann La Roche | Preparación de anticuerpo enriquecida con isoforma y método para obtenerla. |
-
2017
- 2017-01-06 MX MX2018008464A patent/MX2018008464A/es unknown
- 2017-01-06 EP EP17704332.0A patent/EP3400247A1/en not_active Withdrawn
- 2017-01-06 CN CN201780016062.3A patent/CN109641969A/zh active Pending
- 2017-01-06 AU AU2017205477A patent/AU2017205477A1/en not_active Abandoned
- 2017-01-06 JP JP2018535144A patent/JP2019504060A/ja active Pending
- 2017-01-06 WO PCT/US2017/012477 patent/WO2017120435A1/en not_active Ceased
- 2017-01-06 CA CA3010612A patent/CA3010612A1/en not_active Abandoned
- 2017-01-06 US US16/067,196 patent/US20190016753A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO2017120435A1 (en) | 2017-07-13 |
| JP2019504060A (ja) | 2019-02-14 |
| US20190016753A1 (en) | 2019-01-17 |
| CN109641969A (zh) | 2019-04-16 |
| AU2017205477A1 (en) | 2018-07-26 |
| CA3010612A1 (en) | 2017-07-13 |
| EP3400247A1 (en) | 2018-11-14 |
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