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MX2018005386A - Metodos para extraccion de adn libre de celulas para diagnostico prenatal no invasivo. - Google Patents

Metodos para extraccion de adn libre de celulas para diagnostico prenatal no invasivo.

Info

Publication number
MX2018005386A
MX2018005386A MX2018005386A MX2018005386A MX2018005386A MX 2018005386 A MX2018005386 A MX 2018005386A MX 2018005386 A MX2018005386 A MX 2018005386A MX 2018005386 A MX2018005386 A MX 2018005386A MX 2018005386 A MX2018005386 A MX 2018005386A
Authority
MX
Mexico
Prior art keywords
cell
methods
free dna
dna extraction
invasive prenatal
Prior art date
Application number
MX2018005386A
Other languages
English (en)
Inventor
Sun Weimin
Anderson Ben
Strom Charles
Tsao David
Liu Yan
Original Assignee
Quest Diagnostics Invest Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Quest Diagnostics Invest Inc filed Critical Quest Diagnostics Invest Inc
Publication of MX2018005386A publication Critical patent/MX2018005386A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/112—Disease subtyping, staging or classification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

En la presente se proporcionan métodos y sistemas para la extracción de ADN libre de células a partir de muestras biológicas líquidas. Los métodos pueden emplearse para determinar la fracción de ADN fetal y la exploración prenatal no invasiva de aneuploidías fetales y análisis de otros tipos de ADN libre de células.
MX2018005386A 2015-10-26 2016-10-25 Metodos para extraccion de adn libre de celulas para diagnostico prenatal no invasivo. MX2018005386A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562246421P 2015-10-26 2015-10-26
PCT/US2016/058640 WO2017074926A1 (en) 2015-10-26 2016-10-25 Methods for cell-free dna extraction for non-invasive prenatal screening

Publications (1)

Publication Number Publication Date
MX2018005386A true MX2018005386A (es) 2018-06-06

Family

ID=58631101

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2018005386A MX2018005386A (es) 2015-10-26 2016-10-25 Metodos para extraccion de adn libre de celulas para diagnostico prenatal no invasivo.

Country Status (7)

Country Link
US (3) US11485967B2 (es)
EP (1) EP3368667B1 (es)
CN (1) CN108431222A (es)
CA (1) CA3002840C (es)
MX (1) MX2018005386A (es)
SA (1) SA518391425B1 (es)
WO (1) WO2017074926A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020051529A1 (en) 2018-09-07 2020-03-12 Sequenom, Inc. Methods, and systems to detect transplant rejection
CN110172457A (zh) * 2019-06-14 2019-08-27 洛阳爱森生物科技有限公司 一种可用于建库的全血dna提取试剂盒
US20220325272A1 (en) * 2019-09-20 2022-10-13 Siemens Healthcare Diagnostics Inc. Sample preparation apparatus and multi-well plate with pcr chip
WO2021174079A2 (en) 2020-02-28 2021-09-02 Laboratory Corporation Of America Holdings Compositions, methods, and systems for paternity determination
EP4239076A4 (en) * 2020-10-28 2024-07-10 BGI Shenzhen MICROBELT AND PREPARATION METHOD THEREFOR
CN118932079B (zh) * 2024-08-27 2025-03-21 中科(广州)生命科学研究有限公司 一种鉴定生物学亲缘关系的分析方法

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6649419B1 (en) 2000-11-28 2003-11-18 Large Scale Proteomics Corp. Method and apparatus for protein manipulation
WO2008155549A2 (en) 2007-06-19 2008-12-24 Oncomethylome Sciences Sa Improved urine sample collecting and processing
CN113388605A (zh) * 2011-09-26 2021-09-14 凯杰有限公司 用于分离细胞外核酸的快速方法
CA2863215C (en) * 2012-01-30 2021-05-04 Exact Sciences Corporation Modification of dna on magnetic beads
US10619152B2 (en) * 2014-02-10 2020-04-14 Nancy Quan Isolation of cell-free nucleic acids from bodily fluid samples using solid chaotropic agents
CN104164417A (zh) 2014-06-26 2014-11-26 北京圣谷同创科技发展有限公司 一种外周血胎儿游离dna提取方法
US20160312266A1 (en) * 2014-07-28 2016-10-27 Yiding Liu Methods for automated capture and purification of multiple nucleic acid targets from stool samples
CN104789553A (zh) * 2015-04-08 2015-07-22 浙江圣庭生物科技有限公司 一种血液游离dna文库的构建方法
CN104946623A (zh) * 2015-04-08 2015-09-30 浙江圣庭生物科技有限公司 基于Illumina Hiseq 2500测序平台的小片段DNA文库的构建方法

Also Published As

Publication number Publication date
US11485967B2 (en) 2022-11-01
CN108431222A (zh) 2018-08-21
WO2017074926A1 (en) 2017-05-04
SA518391425B1 (ar) 2022-01-25
CA3002840A1 (en) 2017-05-04
EP3368667A1 (en) 2018-09-05
CA3002840C (en) 2024-06-25
US20250136969A1 (en) 2025-05-01
EP3368667A4 (en) 2019-03-27
US20190127728A1 (en) 2019-05-02
EP3368667B1 (en) 2020-12-23
US12134765B2 (en) 2024-11-05
US20230265414A1 (en) 2023-08-24
BR112018008296A2 (pt) 2018-10-30

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