MX2017008923A - Glicoproteinas recombinantes sialiladas con o-glicano y lineas de celulas para producir las mismas. - Google Patents
Glicoproteinas recombinantes sialiladas con o-glicano y lineas de celulas para producir las mismas.Info
- Publication number
- MX2017008923A MX2017008923A MX2017008923A MX2017008923A MX2017008923A MX 2017008923 A MX2017008923 A MX 2017008923A MX 2017008923 A MX2017008923 A MX 2017008923A MX 2017008923 A MX2017008923 A MX 2017008923A MX 2017008923 A MX2017008923 A MX 2017008923A
- Authority
- MX
- Mexico
- Prior art keywords
- recombinant
- ga1nac
- glycans
- cell lines
- recombinant glycoproteins
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/4753—Hepatocyte growth factor; Scatter factor; Tumor cytotoxic factor II
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
- C07K14/811—Serine protease (E.C. 3.4.21) inhibitors
- C07K14/8121—Serpins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/005—Glycopeptides, glycoproteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
- C12Y204/99001—Beta-galactoside alpha-2,6-sialyltransferase (2.4.99.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y204/00—Glycosyltransferases (2.4)
- C12Y204/99—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
- C12Y204/99004—Beta-galactoside alpha-2,3-sialyltransferase (2.4.99.4)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Toxicology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
La presente invención se refiere a líneas de células que se modifican genéticamente para sobreexpresar una ß-galactosido a-2,3-sialiltransferasa 1 (ST3Ga11), de manera preferente ST3Ga11 humana, que se puede usar para la producción de glicoproteínas recombinantes que tienen glicanos Ga1NAc ligados a O sumamente o completamente sialilados (O-glicanos Ga1NAc), de manera preferente O-glicanos Ga1NAc de núcleo 1, así como glicoproteínas recombinantes respectivas. Además, la presente invención se refiere a métodos respectivos para expresar glicoproteínas recombinantes, los métodos para incrementar el grado de sialilación de las glicoproteínas recombinantes, y métodos para disminuir la micro-heterogeneidad de los O-glicanos Ga1NAc. Finalmente, la presente invención se refiere a usos respectivos de las líneas de células anteriores para la producción de glicoproteinas recombinantes, para incrementar el grado de sialilación de las glicoproteinas recombinantes y para disminuir la micro-heterogeneidad de los glicanos Ga1NAc ligados a O de las glicoproteínas recombinantes.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP15000016.4A EP3042952A1 (en) | 2015-01-07 | 2015-01-07 | O-glycan sialylated recombinant glycoproteins and cell lines for producing the same |
| PCT/EP2015/002521 WO2016110302A1 (en) | 2015-01-07 | 2015-12-15 | O-glycan sialylated recombinant glycoproteins and cell lines for producing the same |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| MX2017008923A true MX2017008923A (es) | 2017-10-11 |
| MX387417B MX387417B (es) | 2025-03-18 |
Family
ID=52396403
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2017008923A MX387417B (es) | 2015-01-07 | 2015-12-15 | Glicoproteínas recombinantes sialiladas con o-glicano y líneas de células para producir las mismas. |
Country Status (16)
| Country | Link |
|---|---|
| US (2) | US10081798B2 (es) |
| EP (2) | EP3042952A1 (es) |
| JP (1) | JP6395943B2 (es) |
| KR (1) | KR102013712B1 (es) |
| CN (1) | CN107109370B (es) |
| AU (2) | AU2015376273B2 (es) |
| BR (1) | BR112017014769A2 (es) |
| CA (1) | CA2972932C (es) |
| DK (1) | DK3242935T3 (es) |
| IL (1) | IL253339A0 (es) |
| MX (1) | MX387417B (es) |
| MY (1) | MY187076A (es) |
| RU (1) | RU2714209C2 (es) |
| SG (1) | SG11201704571WA (es) |
| WO (1) | WO2016110302A1 (es) |
| ZA (1) | ZA201703767B (es) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7189767B2 (ja) | 2015-11-19 | 2022-12-14 | 武田薬品工業株式会社 | 組換えヒトｃ１エステラーゼインヒビター及びその使用 |
| EP3382014A1 (en) | 2017-03-29 | 2018-10-03 | CEVEC Pharmaceuticals GmbH | Recombinant glycoproteins with reduced antennary fucosylation |
| ES2933995T3 (es) * | 2018-05-28 | 2023-02-15 | Chr Hansen Hmo Gmbh | Producción fermentativa de sacáridos sialilados |
| US12331320B2 (en) | 2018-10-10 | 2025-06-17 | The Research Foundation For The State University Of New York | Genome edited cancer cell vaccines |
| CN110029129A (zh) * | 2019-03-06 | 2019-07-19 | 华南农业大学 | 一种表达鸡st3gal 1基因的mdck稳转细胞株的构建方法 |
| EP3736286A1 (en) | 2019-05-09 | 2020-11-11 | Biotest AG | Single chain factor viii molecule |
| EP3785726A1 (en) | 2019-09-02 | 2021-03-03 | Biotest AG | Factor viii protein with increased half-life |
| EP4017521A1 (en) | 2019-09-02 | 2022-06-29 | Biotest AG | Factor viii protein with increased half-life |
| US20230190881A1 (en) | 2020-02-17 | 2023-06-22 | Biotest Ag | Subcutaneous administration of factor viii |
| CA3196069A1 (en) | 2020-11-06 | 2022-05-12 | Cho Pharma, Inc. | Immune composition comprising antigen and glycoengineered antibody thereof |
| WO2023069926A1 (en) | 2021-10-18 | 2023-04-27 | Regeneron Pharmaceuticals, Inc. | Eukaryotic cells comprising adenovirus-associated virus polynucleotides |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5262308A (en) | 1992-01-28 | 1993-11-16 | Thomas Jefferson University | Cell lines which constitutively express IGF-1 and IGF-1 R |
| DE19955558C2 (de) | 1999-11-18 | 2003-03-20 | Stefan Kochanek | Permanente Amniozyten-Zelllinie, ihre Herstellung und Verwendung zur Herstellung von Gentransfervektoren |
| RU2463345C2 (ru) * | 2006-07-13 | 2012-10-10 | Вайет | Получение гликопротеинов |
| US20100028949A1 (en) | 2006-12-22 | 2010-02-04 | Vincent Beuger | Shrna-mediated inhibition of expression of alpha 1,6-fucosyltransferase |
| CA2679732A1 (en) | 2007-03-07 | 2008-09-18 | Glycofi, Inc. | Production of glycoproteins with modified fucosylation |
| WO2010012793A1 (en) | 2008-08-01 | 2010-02-04 | Bayer Cropscience Sa | Fungicide aminothiazole derivatives |
| CN101613678A (zh) * | 2009-01-14 | 2009-12-30 | 中国农业科学院哈尔滨兽医研究所 | 稳定表达β-半乳糖苷α-2，3-唾液酸转移酶I(ST3GAL I)的MDCK细胞系的建立 |
| KR101241322B1 (ko) * | 2010-02-08 | 2013-03-11 | 한국과학기술원 | 시알산 함량이 높은 재조합 당단백질의 제조 방법 |
| WO2011101242A1 (en) * | 2010-02-16 | 2011-08-25 | Novo Nordisk A/S | Factor viii molecules with reduced vwf binding |
| TW201209160A (en) | 2010-04-27 | 2012-03-01 | Lonza Ag | Improved glycosylation of proteins in host cells |
| WO2012077128A1 (en) | 2010-12-07 | 2012-06-14 | Intas Biopharmaceuticals Limited | A novel cell line development process to produce recombinant proteins using twin vector expression system |
| DK2661492T3 (en) * | 2011-01-06 | 2018-01-15 | Univ Johns Hopkins | Method for Preparing Recombinant Glycoproteins with Increased Circuit Half Life in Mammalian Cells |
| WO2013093760A2 (en) | 2011-12-19 | 2013-06-27 | Grifols, S.A. | Compositions, methods, and kits for preparing sialylated recombinant proteins |
| WO2014015227A1 (en) | 2012-07-19 | 2014-01-23 | The Scripps Research Institute | Engineering cell surfaces for orthogonal selectability |
| BR112015019348A2 (pt) * | 2013-02-13 | 2017-08-22 | Lab Francais Du Fractionnement | Métodos para produção de proteína com glicosilação modificada e com sialilação aumentada, para aumentar a atividade de sialil transferase na glândula mamária e para produzir sialil transferase, proteína com glicosilação modificada ou proteína com sialilação aumentada, composição, sialil transferase, mamífero transgênico, e, célula epitelial mamária |
-
2015
- 2015-01-07 EP EP15000016.4A patent/EP3042952A1/en not_active Withdrawn
- 2015-12-15 CN CN201580072695.7A patent/CN107109370B/zh active Active
- 2015-12-15 AU AU2015376273A patent/AU2015376273B2/en active Active
- 2015-12-15 JP JP2017536883A patent/JP6395943B2/ja active Active
- 2015-12-15 RU RU2017127276A patent/RU2714209C2/ru active
- 2015-12-15 WO PCT/EP2015/002521 patent/WO2016110302A1/en not_active Ceased
- 2015-12-15 CA CA2972932A patent/CA2972932C/en active Active
- 2015-12-15 MX MX2017008923A patent/MX387417B/es unknown
- 2015-12-15 SG SG11201704571WA patent/SG11201704571WA/en unknown
- 2015-12-15 EP EP15821020.3A patent/EP3242935B1/en active Active
- 2015-12-15 BR BR112017014769-6A patent/BR112017014769A2/pt not_active Application Discontinuation
- 2015-12-15 MY MYPI2017000930A patent/MY187076A/en unknown
- 2015-12-15 DK DK15821020.3T patent/DK3242935T3/da active
- 2015-12-15 KR KR1020177021780A patent/KR102013712B1/ko active Active
- 2015-12-15 US US15/541,565 patent/US10081798B2/en active Active
-
2017
- 2017-06-01 ZA ZA2017/03767A patent/ZA201703767B/en unknown
- 2017-07-05 IL IL253339A patent/IL253339A0/en active IP Right Grant
- 2017-07-27 AU AU2017208292A patent/AU2017208292A1/en not_active Abandoned
-
2018
- 2018-08-21 US US16/107,009 patent/US10793839B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| EP3042952A1 (en) | 2016-07-13 |
| EP3242935A1 (en) | 2017-11-15 |
| RU2017127276A (ru) | 2019-02-07 |
| MX387417B (es) | 2025-03-18 |
| IL253339A0 (en) | 2017-09-28 |
| AU2015376273B2 (en) | 2017-07-27 |
| AU2015376273A1 (en) | 2017-06-08 |
| EP3242935B1 (en) | 2023-11-22 |
| WO2016110302A1 (en) | 2016-07-14 |
| CA2972932C (en) | 2019-08-06 |
| CN107109370B (zh) | 2022-03-22 |
| RU2017127276A3 (es) | 2019-02-07 |
| CN107109370A (zh) | 2017-08-29 |
| RU2714209C2 (ru) | 2020-02-13 |
| US10081798B2 (en) | 2018-09-25 |
| HK1243728A1 (zh) | 2018-07-20 |
| CA2972932A1 (en) | 2016-07-14 |
| BR112017014769A2 (pt) | 2018-01-16 |
| ZA201703767B (en) | 2018-04-25 |
| JP2018500922A (ja) | 2018-01-18 |
| DK3242935T3 (da) | 2024-01-02 |
| US20200095562A1 (en) | 2020-03-26 |
| KR20170094454A (ko) | 2017-08-17 |
| US10793839B2 (en) | 2020-10-06 |
| SG11201704571WA (en) | 2017-07-28 |
| KR102013712B1 (ko) | 2019-08-23 |
| JP6395943B2 (ja) | 2018-09-26 |
| AU2017208292A1 (en) | 2017-08-17 |
| MY187076A (en) | 2021-08-29 |
| US20170342390A1 (en) | 2017-11-30 |
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