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MX2017008036A - Sistemas de modificacion del genoma fungico y metodos de uso. - Google Patents

Sistemas de modificacion del genoma fungico y metodos de uso.

Info

Publication number
MX2017008036A
MX2017008036A MX2017008036A MX2017008036A MX2017008036A MX 2017008036 A MX2017008036 A MX 2017008036A MX 2017008036 A MX2017008036 A MX 2017008036A MX 2017008036 A MX2017008036 A MX 2017008036A MX 2017008036 A MX2017008036 A MX 2017008036A
Authority
MX
Mexico
Prior art keywords
genome
methods
modification
fungical
systems
Prior art date
Application number
MX2017008036A
Other languages
English (en)
Inventor
Ward Michael
Ge Jing
Gu Xiaogang
Mampusti Madrid Susan
Song Danfeng
Song Mingmin
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Publication of MX2017008036A publication Critical patent/MX2017008036A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Se proporcionan composiciones y métodos para la modificación genómica en un sitio diana en el genoma de una célula fúngica. Los aspectos de los métodos y composiciones tratan sobre un sistema de polinucleótido guía/endonucleasa Cas para promover la inserción de un ADN donante en el sitio diana deseado en el genoma de una célula hospedadora fúngica.
MX2017008036A 2014-12-16 2015-12-16 Sistemas de modificacion del genoma fungico y metodos de uso. MX2017008036A (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
CN2014093918 2014-12-16
CN2014093916 2014-12-16
CN2014093914 2014-12-16
PCT/US2015/066195 WO2016100571A1 (en) 2014-12-16 2015-12-16 Fungal genome modification systems and methods of use

Publications (1)

Publication Number Publication Date
MX2017008036A true MX2017008036A (es) 2017-09-28

Family

ID=55083496

Family Applications (3)

Application Number Title Priority Date Filing Date
MX2017007928A MX2017007928A (es) 2014-12-16 2015-12-15 Sistemas de modificación de genoma fúngico y métodos de uso.
MX2017008036A MX2017008036A (es) 2014-12-16 2015-12-16 Sistemas de modificacion del genoma fungico y metodos de uso.
MX2017007930A MX2017007930A (es) 2014-12-16 2015-12-16 Sistemas de modificación de genoma fúngico y métodos de uso.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2017007928A MX2017007928A (es) 2014-12-16 2015-12-15 Sistemas de modificación de genoma fúngico y métodos de uso.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2017007930A MX2017007930A (es) 2014-12-16 2015-12-16 Sistemas de modificación de genoma fúngico y métodos de uso.

Country Status (12)

Country Link
US (3) US11427829B2 (es)
EP (3) EP3234150B1 (es)
JP (3) JP6814142B2 (es)
KR (3) KR102350405B1 (es)
CN (3) CN107667171A (es)
AU (3) AU2015362784B2 (es)
BR (3) BR112017012837A2 (es)
CA (3) CA2971187C (es)
DK (3) DK3234150T3 (es)
FI (2) FI3234150T3 (es)
MX (3) MX2017007928A (es)
WO (3) WO2016100272A1 (es)

Families Citing this family (81)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2734621B1 (en) 2011-07-22 2019-09-04 President and Fellows of Harvard College Evaluation and improvement of nuclease cleavage specificity
US20150044192A1 (en) 2013-08-09 2015-02-12 President And Fellows Of Harvard College Methods for identifying a target site of a cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
US9340800B2 (en) 2013-09-06 2016-05-17 President And Fellows Of Harvard College Extended DNA-sensing GRNAS
US9388430B2 (en) 2013-09-06 2016-07-12 President And Fellows Of Harvard College Cas9-recombinase fusion proteins and uses thereof
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US20150166982A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting pi3k point mutations
EP4079847A1 (en) 2014-07-30 2022-10-26 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
EP3284749B1 (en) * 2015-04-13 2024-08-14 The University of Tokyo Set of polypeptides exhibiting nuclease activity or nickase activity with dependence on light or in presence of drug or suppressing or activating expression of target gene
WO2017019867A1 (en) * 2015-07-28 2017-02-02 Danisco Us Inc Genome editing systems and methods of use
JP7067793B2 (ja) 2015-10-23 2022-05-16 プレジデント アンド フェローズ オブ ハーバード カレッジ 核酸塩基編集因子およびその使用
EP3412765B1 (en) * 2016-02-04 2023-01-18 Kao Corporation Method for producing mutant filamentous fungi
EP3419992B1 (en) 2016-02-22 2020-11-18 Danisco US Inc. Fungal high-level protein production system
WO2017191210A1 (en) * 2016-05-04 2017-11-09 Novozymes A/S Genome editing by crispr-cas9 in filamentous fungal host cells
WO2018015444A1 (en) * 2016-07-22 2018-01-25 Novozymes A/S Crispr-cas9 genome editing with multiple guide rnas in filamentous fungi
IL264565B2 (en) 2016-08-03 2024-07-01 Harvard College Adenosine nucleobase editors and uses thereof
EP3497214B1 (en) 2016-08-09 2023-06-28 President and Fellows of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
US11352647B2 (en) 2016-08-17 2022-06-07 The Broad Institute, Inc. Crispr enzymes and systems
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
WO2018064208A1 (en) 2016-09-28 2018-04-05 The Broad Institute, Inc. Systematic screening and mapping of regulatory elements in non-coding genomic regions, methods, compositions, and applications thereof
WO2018067599A1 (en) 2016-10-04 2018-04-12 Danisco Us Inc. Protein production in filamentous fungal cells in the absence of inducing substrates
KR102622411B1 (ko) 2016-10-14 2024-01-10 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵염기 에디터의 aav 전달
US10745677B2 (en) 2016-12-23 2020-08-18 President And Fellows Of Harvard College Editing of CCR5 receptor gene to protect against HIV infection
JP6839424B2 (ja) * 2016-12-27 2021-03-10 国立大学法人 鹿児島大学 真菌におけるタンパク質の選択的分泌技術
KR20190123328A (ko) 2017-03-09 2019-10-31 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 암 백신
WO2018165504A1 (en) 2017-03-09 2018-09-13 President And Fellows Of Harvard College Suppression of pain by gene editing
EP3592777A1 (en) 2017-03-10 2020-01-15 President and Fellows of Harvard College Cytosine to guanine base editor
US20200063166A1 (en) 2017-03-13 2020-02-27 Dsm Ip Assets B.V. Zinc binuclear cluster transcriptional regulator-deficient strain
SG11201908658TA (en) 2017-03-23 2019-10-30 Harvard College Nucleobase editors comprising nucleic acid programmable dna binding proteins
WO2018197020A1 (en) * 2017-04-27 2018-11-01 Novozymes A/S Genome editing by crispr-cas9 using short donor oligonucleotides
WO2018204777A2 (en) 2017-05-05 2018-11-08 The Broad Institute, Inc. Methods for identification and modification of lncrna associated with target genotypes and phenotypes
WO2018209320A1 (en) 2017-05-12 2018-11-15 President And Fellows Of Harvard College Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation
CN110719956A (zh) * 2017-06-06 2020-01-21 齐默尔根公司 用于改良真菌菌株的高通量基因组工程改造平台
JP7518620B2 (ja) 2017-06-09 2024-07-18 エディタス・メディシン，インコーポレイテッド 操作されたｃａｓ９ヌクレアーゼ
JP2020534795A (ja) 2017-07-28 2020-12-03 プレジデント アンド フェローズ オブ ハーバード カレッジ ファージによって支援される連続的進化（ｐａｃｅ）を用いて塩基編集因子を進化させるための方法および組成物
WO2019139645A2 (en) 2017-08-30 2019-07-18 President And Fellows Of Harvard College High efficiency base editors comprising gam
WO2019046703A1 (en) * 2017-09-01 2019-03-07 Novozymes A/S METHODS OF ENHANCING GENOME EDITION IN FUNGI
KR20250107288A (ko) 2017-10-16 2025-07-11 더 브로드 인스티튜트, 인코퍼레이티드 아데노신 염기 편집제의 용도
EP3724214A4 (en) 2017-12-15 2021-09-01 The Broad Institute Inc. SYSTEMS AND METHODS FOR PREDICTING REPAIR RESULTS IN GENETIC ENGINEERING
CN108085317B (zh) * 2018-01-17 2019-06-21 四川大学 一种特异性靶向变异链球菌gtfB位点的CRISPR-B基因编辑方法及应用
KR102822486B1 (ko) 2018-04-24 2025-06-18 다니스코 유에스 인크. 점도 감소 표현형을 포함하는 사상성 진균 균주
US12157760B2 (en) 2018-05-23 2024-12-03 The Broad Institute, Inc. Base editors and uses thereof
BR112020024839A2 (pt) 2018-06-06 2021-05-18 Zymergen Inc. manipulação de genes envolvidos em transdução de sinal para controlar a morfologia fúngica durante a fermentação e produção
EP3578658A1 (en) * 2018-06-08 2019-12-11 Johann Wolfgang Goethe-Universität Frankfurt Method for generating a gene editing vector with fixed guide rna pairs
EP3820495A4 (en) 2018-07-09 2022-07-20 The Broad Institute Inc. RNA-PROGRAMMABLE EPIGENETIC RNA MODIFIERS AND THEIR USES
WO2020028126A1 (en) 2018-07-30 2020-02-06 Danisco Us Inc Mutant and genetically modified filamentous fungal strains comprising enhanced protein productivity phenotypes and methods thereof
CA3116553A1 (en) 2018-10-16 2020-04-23 Blueallele, Llc Methods for targeted insertion of dna in genes
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
US20220010305A1 (en) * 2018-10-31 2022-01-13 Novozymes A/S Genome Editing by Guided Endonuclease and Single-stranded Oligonucleotide
AU2019398351B2 (en) 2018-12-14 2026-01-29 Pioneer Hi-Bred International, Inc. Novel CRISPR-Cas systems for genome editing
US12264323B2 (en) 2018-12-17 2025-04-01 The Broad Institute, Inc. CRISPR CPF1 direct repeat variants
WO2020154500A1 (en) 2019-01-23 2020-07-30 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
US12215382B2 (en) 2019-03-01 2025-02-04 The General Hospital Corporation Liver protective MARC variants and uses thereof
US12534714B2 (en) 2019-03-18 2026-01-27 The Broad Institute, Inc. Type VII CRISPR proteins and systems
WO2020191246A1 (en) 2019-03-19 2020-09-24 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
US12473543B2 (en) 2019-04-17 2025-11-18 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
US20220235340A1 (en) 2019-05-20 2022-07-28 The Broad Institute, Inc. Novel crispr-cas systems and uses thereof
US20220298501A1 (en) 2019-08-30 2022-09-22 The Broad Institute, Inc. Crispr-associated mu transposase systems
WO2021072328A1 (en) 2019-10-10 2021-04-15 The Broad Institute, Inc. Methods and compositions for prime editing rna
US20220389474A1 (en) 2019-10-28 2022-12-08 Danisco Us Inc. Microbial host cells for the production of heterologous cyanuric acid hydrolases and biuret hydrolases
KR20220097451A (ko) 2019-11-08 2022-07-07 다니스코 유에스 인크. 향상된 단백질 생산성 표현형을 포함하는 진균 균주 및 이의 방법
US20230174998A1 (en) 2020-04-22 2023-06-08 Danisco Us Inc. Compositions and methods for enhanced protein production in filamentous fungal cells
GB2614813B (en) 2020-05-08 2025-05-07 Harvard College Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence
WO2021242774A1 (en) * 2020-05-26 2021-12-02 Zymergen Inc. Methods of transient protein and gene expression in cells
US11479779B2 (en) 2020-07-31 2022-10-25 Zymergen Inc. Systems and methods for high-throughput automated strain generation for non-sporulating fungi
EP4373943A2 (en) 2021-07-19 2024-05-29 Danisco Us Inc Compositions and methods for enhanced protein production in fungal cells
WO2023023644A1 (en) 2021-08-20 2023-02-23 Danisco Us Inc. Polynucleotides encoding novel nucleases, compositions thereof and methods thereof for eliminating dna from protein preparations
EP4399295A1 (en) 2021-09-09 2024-07-17 International N&H Denmark ApS Over expression of foldases and chaperones improves protein production
EP4441202A1 (en) 2021-12-01 2024-10-09 Danisco US Inc. Compositions and methods for enhanced protein production in fungal cells
CN114410635B (zh) * 2022-03-29 2022-06-14 中国科学院天津工业生物技术研究所 威尼斯镰刀菌内源U6启动子及其基于CRISPR/Cas9的基因编辑方法
AU2023306398A1 (en) 2022-07-15 2025-01-23 International N&H Denmark Aps Improved enzymatic modification of phospholipids in food
KR20250102093A (ko) 2022-11-11 2025-07-04 다니스코 유에스 인크. 향상된 단백질 생산성 표현형을 포함하는 사상 진균 균주 및 이의 방법
JPWO2024122646A1 (es) * 2022-12-08 2024-06-13
JP2026500088A (ja) 2022-12-19 2026-01-06 ダニスコ・ユーエス・インク 一貫したタンパク質を生成するための組換え真菌株及びその方法
CN120769861A (zh) 2023-02-08 2025-10-10 丹尼斯科美国公司 用于在丝状真菌细胞中生产异源球蛋白的组合物和方法
CN121039276A (zh) 2023-03-27 2025-11-28 丹尼斯科美国公司 使用表达来自帽铁形嘉利翁氏菌的RuBisCo的酵母减少发酵甘油和乙酸
KR20250000949A (ko) * 2023-06-26 2025-01-06 전북대학교산학협력단 시그마-54 및 MmoR을 통한 선택적 메탄 모노옥시게나제의 고효율 생산 방법
WO2025007098A1 (en) 2023-06-30 2025-01-02 Danisco Us Inc. Recombinant fungal cells and methods thereof for industrial scale production of lectins
CN116769781B (zh) * 2023-08-16 2023-10-24 中国科学院天津工业生物技术研究所 一种来源于粗壮脉纹孢菌的启动子及其应用
WO2025059533A1 (en) 2023-09-13 2025-03-20 The Broad Institute, Inc. Crispr enzymes and systems
WO2025207802A1 (en) 2024-03-27 2025-10-02 Nutrition & Biosciences USA 4, Inc. Recombinant microbial strains comprising reduced beta-oxidation phenotypes and methods thereof

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5107065A (en) 1986-03-28 1992-04-21 Calgene, Inc. Anti-sense regulation of gene expression in plant cells
US5290474A (en) 1990-10-05 1994-03-01 Genencor International, Inc. Detergent composition for treating cotton-containing fabrics containing a surfactant and a cellulase composition containing endolucanase III from trichoderma ssp
AU1334899A (en) * 1997-12-22 1999-07-12 De Groot, Marcellus Johannes A. A process for site-directed integration of multiple copies of a gene in a mould
ATE524491T1 (de) 2003-05-29 2011-09-15 Genencor Int Neue trichoderma-gene
EP1685244B8 (en) 2003-11-21 2012-04-25 Danisco US Inc. Expression of granular starch hydrolyzing enzymes in trichoderma and process for producing glucose from granular starch substrates
EP1751281A2 (en) 2004-05-27 2007-02-14 Genencor International, Inc. Aspergillus kawachi acid-stable alpha amylase and applications in granular starch hydrolysis
EP2576796B1 (en) 2010-06-03 2017-03-29 Danisco US Inc. Filamentous fungal host strains and dna constructs, and methods of use thereof
WO2013141680A1 (en) 2012-03-20 2013-09-26 Vilnius University RNA-DIRECTED DNA CLEAVAGE BY THE Cas9-crRNA COMPLEX
EP2912175B1 (en) * 2012-10-23 2018-08-22 Toolgen Incorporated Composition for cleaving a target dna comprising a guide rna specific for the target dna and cas protein-encoding nucleic acid or cas protein, and use thereof
US8697359B1 (en) * 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
US20140189896A1 (en) * 2012-12-12 2014-07-03 Feng Zhang Crispr-cas component systems, methods and compositions for sequence manipulation
CA2913869C (en) * 2013-05-29 2023-01-24 Cellectis New compact scaffold of cas9 in the type ii crispr system
ES2670531T3 (es) * 2013-05-29 2018-05-30 Cellectis S.A. Un método para producir una escisión de ADN precisa utilizando la actividad nickasa de Cas9
WO2015054507A1 (en) * 2013-10-10 2015-04-16 Pronutria, Inc. Nutritive polypeptide production systems, and methods of manufacture and use thereof
MX2016007326A (es) * 2013-12-12 2017-02-13 Broad Inst Inc Composiciones y metodos de uso de sistemas crispr-cas en desordenes debidos a repeticion de nucleotidos.
US20170088845A1 (en) * 2014-03-14 2017-03-30 The Regents Of The University Of California Vectors and methods for fungal genome engineering by crispr-cas9
CA2954974A1 (en) * 2014-07-21 2016-01-28 Glykos Finland Oy Production of glycoproteins with mammalian-like n-glycans in filamentous fungi
US10513711B2 (en) * 2014-08-13 2019-12-24 Dupont Us Holding, Llc Genetic targeting in non-conventional yeast using an RNA-guided endonuclease
DK3242950T3 (da) * 2015-01-06 2021-12-20 Dsm Ip Assets Bv Crispr-cas-system til en trådformet svampeværtscelle

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