[go: up one dir, main page]

MX2017007503A - Metodos novedosos para conjugacion de polipeptido mediada por enzimas usando sortasa. - Google Patents

Metodos novedosos para conjugacion de polipeptido mediada por enzimas usando sortasa.

Info

Publication number
MX2017007503A
MX2017007503A MX2017007503A MX2017007503A MX2017007503A MX 2017007503 A MX2017007503 A MX 2017007503A MX 2017007503 A MX2017007503 A MX 2017007503A MX 2017007503 A MX2017007503 A MX 2017007503A MX 2017007503 A MX2017007503 A MX 2017007503A
Authority
MX
Mexico
Prior art keywords
seq
polypeptide
sortase
novel methods
amino acid
Prior art date
Application number
MX2017007503A
Other languages
English (en)
Other versions
MX381723B (es
Inventor
Kratzsch Peter
Boenitz-Dulat Mara
SCHATTE Martin
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of MX2017007503A publication Critical patent/MX2017007503A/es
Publication of MX381723B publication Critical patent/MX381723B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6472—Cysteine endopeptidases (3.4.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/22—Cysteine endopeptidases (3.4.22)
    • C12Y304/2207—Sortase A (3.4.22.70)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Oncology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

En este documento se reporta de un método para producir un producto de conjugación enzimática de dos polipéptidos que comprende la incubación de un primer polipéptido que comprende la secuencia de aminoácidos LPXTG (SEC ID NO: 20, en donde X puede ser cualquier residuo de aminoácido), un segundo polipéptido tiene una secuencia de aminoácidos de oligo-alanina Am (m = 2 (SEC ID NO: 26) , o 3 (SEC ID NO: 27) o 4 (SEC ID NO: 28), o 5 (SEC ID NO: 29)) en su extremo N-terminal, un tercer polipéptido con actividad sortasa que se deriva de sortasa A de Staphylococcus aureus, y recuperar el conjugado de la mezcla de reacción y produciendo de este modo el producto de conjugación enzimática de dos polipéptidos.
MX2017007503A 2014-12-17 2015-12-14 Metodos novedosos para conjugacion de polipeptido mediada por enzimas usando sortasa. MX381723B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP14198532 2014-12-17
PCT/EP2015/079617 WO2016096741A1 (en) 2014-12-17 2015-12-14 Novel methods for enzyme mediated polypeptide conjugation using sortase

Publications (2)

Publication Number Publication Date
MX2017007503A true MX2017007503A (es) 2017-10-04
MX381723B MX381723B (es) 2025-03-13

Family

ID=52338848

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017007503A MX381723B (es) 2014-12-17 2015-12-14 Metodos novedosos para conjugacion de polipeptido mediada por enzimas usando sortasa.

Country Status (11)

Country Link
US (1) US10190182B2 (es)
EP (1) EP3233905B1 (es)
JP (1) JP6618539B2 (es)
KR (1) KR20170094216A (es)
CN (1) CN107001447B (es)
BR (1) BR112017010775A2 (es)
CA (1) CA2966287A1 (es)
MX (1) MX381723B (es)
RU (1) RU2714963C2 (es)
SG (1) SG11201704625UA (es)
WO (1) WO2016096741A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110295157A (zh) * 2016-08-25 2019-10-01 北京大学 一种金黄色葡萄球菌分选酶a高效突变体
CN116888258A (zh) * 2021-02-04 2023-10-13 西湖生物医药科技(杭州)有限公司 修饰的红细胞及其治疗高尿酸血症和痛风的用途
CN118786207A (zh) * 2022-01-12 2024-10-15 西湖生物医药科技(上海)有限公司 经修饰的细胞及其用途
CN114487409B (zh) * 2022-04-14 2022-07-19 启德医药科技（苏州）有限公司 一种转肽酶活性的检测方法及检测试剂盒
CN116949004B (zh) * 2023-07-13 2024-04-09 苏州源启生物科技有限公司 一种转肽酶Sortase A及其制备方法和应用

Family Cites Families (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4150149A (en) 1976-11-29 1979-04-17 Professional Staff Association Of The Los Angeles County Harbor General Hospital Method and means for the early detection and diagnosis of certain types of cancers
US4275149A (en) 1978-11-24 1981-06-23 Syva Company Macromolecular environment control in specific receptor assays
US4318980A (en) 1978-04-10 1982-03-09 Miles Laboratories, Inc. Heterogenous specific binding assay employing a cycling reactant as label
US4444744A (en) 1980-03-03 1984-04-24 Goldenberg Milton David Tumor localization and therapy with labeled antibodies to cell surface antigens
US4361544A (en) 1980-03-03 1982-11-30 Goldenberg Milton David Tumor localization and therapy with labeled antibodies specific to intracellular tumor-associated markers
US5316757A (en) 1984-10-18 1994-05-31 Board Of Regents, The University Of Texas System Synthesis of polyazamacrocycles with more than one type of side-chain chelating groups
US5342606A (en) 1984-10-18 1994-08-30 Board Of Regents, The University Of Texas System Polyazamacrocyclic compounds for complexation of metal ions
US4737456A (en) 1985-05-09 1988-04-12 Syntex (U.S.A.) Inc. Reducing interference in ligand-receptor binding assays
US5959177A (en) 1989-10-27 1999-09-28 The Scripps Research Institute Transgenic plants expressing assembled secretory antibodies
WO1993006217A1 (en) 1991-09-19 1993-04-01 Genentech, Inc. EXPRESSION IN E. COLI OF ANTIBODY FRAGMENTS HAVING AT LEAST A CYSTEINE PRESENT AS A FREE THIOL, USE FOR THE PRODUCTION OF BIFUNCTIONAL F(ab')2 ANTIBODIES
US5480990A (en) 1991-12-10 1996-01-02 The Dow Chemical Company Bicyclopolyazamacrocyclocarboxylic acid complexes for use as contrast agents
US5428139A (en) 1991-12-10 1995-06-27 The Dow Chemical Company Bicyclopolyazamacrocyclophosphonic acid complexes for use as radiopharmaceuticals
US5739294A (en) 1991-12-10 1998-04-14 The Dow Chemical Company Bicyclopol yazamacrocyclophosphonic acid complexes for use as contrast agents
ZA932522B (en) 1992-04-10 1993-12-20 Res Dev Foundation Immunotoxins directed against c-erbB-2(HER/neu) related surface antigens
ATE139900T1 (de) 1992-11-13 1996-07-15 Idec Pharma Corp Therapeutische verwendung von chimerischen und markierten antikörper gegen menschlichen b lymphozyt beschränkter differenzierung antigen für die behandlung von b-zell-lymphoma
US5462725A (en) 1993-05-06 1995-10-31 The Dow Chemical Company 2-pyridylmethylenepolyazamacrocyclophosphonic acids, complexes and derivatives thereof, for use as contrast agents
US5385893A (en) 1993-05-06 1995-01-31 The Dow Chemical Company Tricyclopolyazamacrocyclophosphonic acids, complexes and derivatives thereof, for use as contrast agents
US5789199A (en) 1994-11-03 1998-08-04 Genentech, Inc. Process for bacterial production of polypeptides
US5840523A (en) 1995-03-01 1998-11-24 Genetech, Inc. Methods and compositions for secretion of heterologous polypeptides
US5834456A (en) 1996-02-23 1998-11-10 The Dow Chemical Company Polyazamacrocyclofluoromonoalkylphosphonic acids, and their complexes, for use as contrast agents
US6040498A (en) 1998-08-11 2000-03-21 North Caroline State University Genetically engineered duckweed
US6528624B1 (en) 1998-04-02 2003-03-04 Genentech, Inc. Polypeptide variants
KR100797667B1 (ko) 1999-10-04 2008-01-23 메디카고 인코포레이티드 외래 유전자의 전사를 조절하는 방법
US7125978B1 (en) 1999-10-04 2006-10-24 Medicago Inc. Promoter for regulating expression of foreign genes
US20100111856A1 (en) 2004-09-23 2010-05-06 Herman Gill Zirconium-radiolabeled, cysteine engineered antibody conjugates
UA95958C2 (en) * 2006-05-30 2011-09-26 Дженентек, Инк. Antibody that binds to cd22, immunoconjugates and uses therefor
WO2010087994A2 (en) 2009-01-30 2010-08-05 Whitehead Institute For Biomedical Research Methods for ligation and uses thereof
EP2403530B1 (en) 2009-02-27 2016-05-11 Massachusetts Institute of Technology Engineered proteins with high affinity for dota chelates
CA2840409A1 (en) 2011-06-28 2013-01-03 Whitehead Institute For Biomedical Research Using sortases to install click chemistry handles for protein ligation
RU2639287C2 (ru) * 2012-06-27 2017-12-20 Ф. Хоффманн-Ля Рош Аг Способ отбора и получения высокоселективных и мультиспецифичных нацеливающих групп с заданными свойствами, включающих по меньшей мере две различные связывающие группировки, и их применения

Also Published As

Publication number Publication date
KR20170094216A (ko) 2017-08-17
EP3233905A1 (en) 2017-10-25
BR112017010775A2 (pt) 2018-02-27
CA2966287A1 (en) 2016-06-23
CN107001447A (zh) 2017-08-01
JP2018500901A (ja) 2018-01-18
JP6618539B2 (ja) 2019-12-11
RU2714963C2 (ru) 2020-02-21
RU2017122029A (ru) 2019-01-17
EP3233905B1 (en) 2020-01-29
RU2017122029A3 (es) 2019-07-17
US10190182B2 (en) 2019-01-29
MX381723B (es) 2025-03-13
SG11201704625UA (en) 2017-07-28
CN107001447B (zh) 2021-07-23
US20170292166A1 (en) 2017-10-12
WO2016096741A1 (en) 2016-06-23

Similar Documents

Publication Publication Date Title
EP4442816A3 (en) Mutated glycoprotein of vesicular stomatitis virus
ES2721309T3 (es) Composición para preparar tagatosa y método para preparar tagatosa a partir de fructosa
MY186296A (en) Novel promoter and use thereof
WO2020030984A3 (en) Compositions and methods for genome engineering with cas12a proteins
MX2017007503A (es) Metodos novedosos para conjugacion de polipeptido mediada por enzimas usando sortasa.
MX377162B (es) Nuevas enzimas y sistemas crispr
CO2017004318A2 (es) Variantes de interferon α2b
SG10201805672UA (en) Compositions and methods for biological production of amino acids in hydrogenotrophic microorganisms
MX2017016105A (es) Polinucleotidos y polipeptidos de adenovirus.
WO2015130846A3 (en) Compositions and methods for the site-specific modification of polypeptides
WO2015185758A3 (en) Preparation comprising factor viii and von willebrand factor peptides
AU2015212749A8 (en) Protein
NZ783737A (en) Novel b-galactosidase
EP4374913A3 (en) Novel human serum albumin mutant
PH12015502071B1 (en) Nutritional compositions containing a peptide component with anti-inflammatory properties and uses thereof
AU2018280528A8 (en) Method for improving transformation efficiency of plant and method for transforming plant
MY195726A (en) Steviol Glycoside Transport
EA201692439A1 (ru) Пролин-специфичная эндопротеаза и ее применение
EA201591609A1 (ru) Варианты альфа-амилазы
EP4043558A3 (en) Kaurenoic acid hydroxylases
PH12017502323B1 (en) Novel xylanase
EP3925968A3 (en) Hsp70-derived peptide, pharmaceutical composition for treating or preventing cancer using same, immunity inducer, and method for producing antigen-presenting cell
EP4282960A3 (en) Modified lipase and use thereof
AR101146A1 (es) Proteína con actividad xilanasa
MX2017007204A (es) Reaccion enzimatica en un solo recipiente para conjugacion de polipeptido doble en un solo paso usando sortasa.