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MX2017005306A - Novedosos sitios de integracion en celulas cho y usos de estos. - Google Patents

Novedosos sitios de integracion en celulas cho y usos de estos.

Info

Publication number
MX2017005306A
MX2017005306A MX2017005306A MX2017005306A MX2017005306A MX 2017005306 A MX2017005306 A MX 2017005306A MX 2017005306 A MX2017005306 A MX 2017005306A MX 2017005306 A MX2017005306 A MX 2017005306A MX 2017005306 A MX2017005306 A MX 2017005306A
Authority
MX
Mexico
Prior art keywords
expression
integration sites
enhanced
eukaryotic
novel cho
Prior art date
Application number
MX2017005306A
Other languages
English (en)
Inventor
Chen Gang
Burakov Darya
Shen Ying
P Fandl James
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=54602002&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2017005306(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of MX2017005306A publication Critical patent/MX2017005306A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Saccharide Compounds (AREA)

Abstract

Se proporcionan secuencias de nucleótidos potenciadoras de la expresión para sistemas de expresión en eucariotas que permiten la expresión estable y aumentada de proteínas recombinantes en células eucariotas. Se proporcionan sitios de integración genómica que proporcionan expresión aumentada y métodos de uso de estos para la expresión de un gen de interés en una célula eucariota. Se proporcionan loci cromosómicos, secuencia, y vectores para la expresión estable y aumentada de genes en células eucariotas.
MX2017005306A 2014-10-23 2015-10-21 Novedosos sitios de integracion en celulas cho y usos de estos. MX2017005306A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462067774P 2014-10-23 2014-10-23
PCT/US2015/056653 WO2016064999A1 (en) 2014-10-23 2015-10-21 Novel cho integration sites and uses thereof

Publications (1)

Publication Number Publication Date
MX2017005306A true MX2017005306A (es) 2018-01-09

Family

ID=54602002

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2017005306A MX2017005306A (es) 2014-10-23 2015-10-21 Novedosos sitios de integracion en celulas cho y usos de estos.

Country Status (16)

Country Link
US (5) US9816110B2 (es)
EP (1) EP3209785B1 (es)
JP (1) JP6668340B2 (es)
KR (1) KR102243243B1 (es)
CN (1) CN107109434A (es)
AR (1) AR102420A1 (es)
AU (1) AU2015335921B2 (es)
BR (1) BR112017008022A2 (es)
CA (1) CA2965495C (es)
EA (1) EA037255B1 (es)
IL (1) IL251674B (es)
MX (1) MX2017005306A (es)
MY (1) MY184103A (es)
SG (1) SG11201703149RA (es)
TW (2) TWI747808B (es)
WO (1) WO2016064999A1 (es)

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CN111886244A (zh) * 2017-12-22 2020-11-03 豪夫迈·罗氏有限公司 核酸的靶向整合
WO2020084034A1 (en) 2018-10-26 2020-04-30 F. Hoffmann-La Roche Ag Multispecific antibody screening method using recombinase mediated cassette exchange
CN109136193B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006884764-1稳定表达蛋白质的应用
CN109295092B (zh) * 2018-10-30 2021-06-29 江南大学 一种cho细胞基因组内nw_003613638-1稳定表达蛋白质的应用
CN109337927B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006880285-1稳定表达蛋白质的应用
CN109207432B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006883358-1稳定表达蛋白质的应用
CN109321604B (zh) * 2018-10-30 2021-07-06 江南大学 一种cho细胞基因组内nw_006882077-1稳定表达蛋白质的应用
CN109295093B (zh) * 2018-10-30 2021-08-03 江南大学 一种cho细胞基因组内nw_006882456-1稳定表达蛋白质的应用
CA3123448A1 (en) * 2018-12-21 2020-06-25 Genentech, Inc. Targeted integration of nucleic acids
AU2020252840B2 (en) * 2019-04-02 2025-01-23 Chugai Seiyaku Kabushiki Kaisha Method of introducing target-specific foreign gene
KR20220025806A (ko) 2019-06-26 2022-03-03 제넨테크, 인크. 핵산의 무작위 구성 표적화 통합
EP3901266A1 (en) * 2020-04-22 2021-10-27 LEK Pharmaceuticals d.d. Super-enhancers for recombinant gene expression in cho cells
JP2023529376A (ja) * 2020-06-02 2023-07-10 キャタレント ファーマ ソリューションズ リミテッド ライアビリティ カンパニー タンパク質製造のための核酸コンストラクト
MX2022015205A (es) * 2020-06-24 2023-01-05 Genentech Inc Integracion dirigida de acidos nucleicos.
HRP20250766T1 (hr) 2020-10-15 2025-08-15 F. Hoffmann - La Roche Ag Konstrukti nukleinske kiseline za transkripciju va rna
CN113755447B (zh) * 2021-09-23 2022-04-29 云舟生物科技(广州)股份有限公司 一种用于生产腺病毒的293a细胞株及其制备与应用
CA3235563A1 (en) 2021-10-18 2023-04-27 Regeneron Pharmaceuticals, Inc. Controlled transcription of polynucleotides
US12234472B2 (en) 2021-10-18 2025-02-25 Regeneron Pharmaceuticals, Inc. Eukaryotic cells comprising adenovirus-associated virus polynucleotides
KR20240099242A (ko) 2021-10-18 2024-06-28 리제너론 파마슈티칼스 인코포레이티드 안정적인 통합 부위를 생산하기 위한 통합된 cas9 유전자를 포함하는 포유동물 세포, 및 안정적인 통합 부위 및 기타 부위를 포함하는 포유동물 세포
CN118613589A (zh) * 2022-01-28 2024-09-06 纽约市哥伦比亚大学理事会 用于异种移植的基因修饰
CN117305334B (zh) * 2022-06-21 2024-11-08 深圳太力生物技术有限责任公司 靶向整合细胞及其制备方法、生产目标基因表达产物的方法
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Also Published As

Publication number Publication date
IL251674A0 (en) 2017-06-29
JP2017535258A (ja) 2017-11-30
IL251674B (en) 2020-10-29
JP6668340B2 (ja) 2020-03-18
US20200002732A1 (en) 2020-01-02
MY184103A (en) 2021-03-18
CA2965495C (en) 2022-05-24
EA201790698A1 (ru) 2018-01-31
CN107109434A (zh) 2017-08-29
US20240018553A1 (en) 2024-01-18
US20160115502A1 (en) 2016-04-28
KR102243243B1 (ko) 2021-04-22
TW202144576A (zh) 2021-12-01
US20180030480A1 (en) 2018-02-01
EP3209785B1 (en) 2019-07-31
BR112017008022A2 (pt) 2018-02-20
AR102420A1 (es) 2017-03-01
WO2016064999A1 (en) 2016-04-28
CA2965495A1 (en) 2016-04-28
US9816110B2 (en) 2017-11-14
AU2015335921B2 (en) 2020-06-25
TWI747808B (zh) 2021-12-01
EP3209785A1 (en) 2017-08-30
SG11201703149RA (en) 2018-02-27
US11268109B2 (en) 2022-03-08
KR20170096999A (ko) 2017-08-25
TW201629229A (zh) 2016-08-16
TWI780847B (zh) 2022-10-11
EA037255B1 (ru) 2021-02-26
AU2015335921A1 (en) 2017-05-04
US20210171984A1 (en) 2021-06-10
US11788102B2 (en) 2023-10-17

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