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MX2016016905A - Ensamblaje de adn mediado por nucleasa. - Google Patents

Ensamblaje de adn mediado por nucleasa.

Info

Publication number
MX2016016905A
MX2016016905A MX2016016905A MX2016016905A MX2016016905A MX 2016016905 A MX2016016905 A MX 2016016905A MX 2016016905 A MX2016016905 A MX 2016016905A MX 2016016905 A MX2016016905 A MX 2016016905A MX 2016016905 A MX2016016905 A MX 2016016905A
Authority
MX
Mexico
Prior art keywords
sequences
assemble
overlapping
create
complementary
Prior art date
Application number
MX2016016905A
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English (en)
Other versions
MX379237B (es
Inventor
J Murphy Andrew
Macdonald Lynn
Mcwhirter John
Venus Lai Ka-Man
F Rojas Jose
M Valenzuela David
Schoenherr Chris
Momont Corey
S Warshaw Gregg
Montagna Caitlin
Original Assignee
Regeneron Pharma
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Publication date
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of MX2016016905A publication Critical patent/MX2016016905A/es
Publication of MX379237B publication Critical patent/MX379237B/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1031—Mutagenizing nucleic acids mutagenesis by gene assembly, e.g. assembly by oligonucleotide extension PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

En la presente invención se proporcionan métodos para ensamblar al menos dos ácidos nucleicos mediante el uso de un agente nucleasa específico de secuencia (p. ej., un complejo ARNg-Cas) para crear secuencias de extremo que tienen complementariedad y ensamblar posteriormente las secuencias complementarias superpuestas. El agente nucleasa (p. ej., un complejo ARNgA-Cas) puede crear rupturas bicatenarias en el ADNbc con el propósito de crear secuencias de extremos superpuestas o puede crear mellas en cada cadena para producir secuencias de extremo salientes complementarias. El ensamblaje mediante el uso del método descrito en el presente descripción puede ensamblar cualquier ácido nucleico que tenga secuencias superpuestas o puede usar un acoplador oligo para ensamblar secuencias sin extremos complementarios.
MX2016016905A 2014-06-23 2015-06-23 Ensamblaje de adn mediado por nucleasa. MX379237B (es)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201462015809P 2014-06-23 2014-06-23
US201462016400P 2014-06-24 2014-06-24
US201462036983P 2014-08-13 2014-08-13
PCT/US2015/037199 WO2015200334A1 (en) 2014-06-23 2015-06-23 Nuclease-mediated dna assembly

Publications (2)

Publication Number Publication Date
MX2016016905A true MX2016016905A (es) 2017-08-10
MX379237B MX379237B (es) 2025-03-10

Family

ID=53525285

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2016016905A MX379237B (es) 2014-06-23 2015-06-23 Ensamblaje de adn mediado por nucleasa.
MX2021000857A MX384887B (es) 2014-06-23 2015-06-23 Ensamblaje de adn mediado por nucleasa.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2021000857A MX384887B (es) 2014-06-23 2015-06-23 Ensamblaje de adn mediado por nucleasa.

Country Status (25)

Country Link
US (6) US9738897B2 (es)
EP (3) EP3354732B1 (es)
JP (4) JP6336140B2 (es)
KR (2) KR101822902B1 (es)
CN (2) CN106715694B (es)
AU (3) AU2015280120B2 (es)
BR (1) BR112016030145A8 (es)
CA (1) CA2953499C (es)
CY (2) CY1120520T1 (es)
DK (2) DK3354732T3 (es)
ES (2) ES2781323T3 (es)
HR (1) HRP20200523T1 (es)
HU (1) HUE049405T2 (es)
IL (1) IL249612B (es)
LT (1) LT3354732T (es)
MX (2) MX379237B (es)
NZ (1) NZ765591A (es)
PL (2) PL3354732T3 (es)
PT (2) PT3155099T (es)
RS (1) RS60366B1 (es)
RU (1) RU2707911C2 (es)
SG (2) SG10201803444YA (es)
SI (1) SI3354732T1 (es)
SM (1) SMT202000174T1 (es)
WO (1) WO2015200334A1 (es)

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