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MX2016013965A - Metodos de purificacion de arn mensajero. - Google Patents

Metodos de purificacion de arn mensajero.

Info

Publication number
MX2016013965A
MX2016013965A MX2016013965A MX2016013965A MX2016013965A MX 2016013965 A MX2016013965 A MX 2016013965A MX 2016013965 A MX2016013965 A MX 2016013965A MX 2016013965 A MX2016013965 A MX 2016013965A MX 2016013965 A MX2016013965 A MX 2016013965A
Authority
MX
Mexico
Prior art keywords
mrna
methods
messenger rna
purification
membrane
Prior art date
Application number
MX2016013965A
Other languages
English (en)
Other versions
MX373952B (es
Inventor
Michael Heartlein
Frank Derosa
Anusha Dias
Karve Shrirang
Original Assignee
Shire Human Genetic Therapies
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=54333288&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2016013965(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Shire Human Genetic Therapies filed Critical Shire Human Genetic Therapies
Publication of MX2016013965A publication Critical patent/MX2016013965A/es
Publication of MX373952B publication Critical patent/MX373952B/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00Processes for the preparation of sugar derivatives
    • C07H1/06Separation; Purification
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Saccharide Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La presente invención provee, entre otras cosas, métodos para purificar ARN mensajero (ARNm) que incluyen las etapas de (a) precipitación de ARNm de una preparación impura, (b) sometimiento de la preparación impura que comprende ARNm a un proceso de purificación que involucra la filtración de membrana, de forma que una membrana captura el ARNm precipitado y (c) elución del ARNm precipitado capturado de la membrana mediante la resolubilización del ARNm, lo que resulta en una solución de ARNm purificado; en algunas modalidades, la filtración de flujo tangencial es un proceso de purificación que involucra la filtración de membrana adecuada para la presente invención.
MX2016013965A 2014-04-25 2015-04-24 Métodos de purificación de arn mensajero. MX373952B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461984503P 2014-04-25 2014-04-25
PCT/US2015/027563 WO2015164773A1 (en) 2014-04-25 2015-04-24 Methods for purification of messenger rna

Publications (2)

Publication Number Publication Date
MX2016013965A true MX2016013965A (es) 2017-01-09
MX373952B MX373952B (es) 2020-07-13

Family

ID=54333288

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2016013965A MX373952B (es) 2014-04-25 2015-04-24 Métodos de purificación de arn mensajero.
MX2020004603A MX2020004603A (es) 2014-04-25 2016-10-24 Metodos de purificacion de arn mensajero.

Family Applications After (1)

Application Number Title Priority Date Filing Date
MX2020004603A MX2020004603A (es) 2014-04-25 2016-10-24 Metodos de purificacion de arn mensajero.

Country Status (16)

Country Link
US (6) US9850269B2 (es)
EP (2) EP3636742B1 (es)
JP (5) JP6571679B2 (es)
KR (3) KR20250005450A (es)
CN (3) CN110511927A (es)
AU (3) AU2015249312B2 (es)
BR (1) BR112016024632A2 (es)
CA (1) CA2944800A1 (es)
DK (2) DK3636742T3 (es)
EA (1) EA201691696A1 (es)
ES (1) ES2750661T3 (es)
IL (1) IL247973A0 (es)
MX (2) MX373952B (es)
PT (1) PT3134506T (es)
SG (1) SG11201608725YA (es)
WO (1) WO2015164773A1 (es)

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Publication number Priority date Publication date Assignee Title
US12458604B2 (en) 2020-10-14 2025-11-04 The Trustees Of The University Of Pennsylvania Methods of lipid nanoparticle manufacture and compositions derived therefrom

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