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MX2016001678A - Construcciones de expresion y metodos para expresar polipeptidos en celulas eucariotas. - Google Patents

Construcciones de expresion y metodos para expresar polipeptidos en celulas eucariotas.

Info

Publication number
MX2016001678A
MX2016001678A MX2016001678A MX2016001678A MX2016001678A MX 2016001678 A MX2016001678 A MX 2016001678A MX 2016001678 A MX2016001678 A MX 2016001678A MX 2016001678 A MX2016001678 A MX 2016001678A MX 2016001678 A MX2016001678 A MX 2016001678A
Authority
MX
Mexico
Prior art keywords
expression
methods
eukaryotic cells
expression constructs
polypeptides
Prior art date
Application number
MX2016001678A
Other languages
English (en)
Inventor
Christel Aebischer-Gumy
Bertschinger Martin
Moretti Pierre
Original Assignee
Glenmark Pharmaceuticals Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Glenmark Pharmaceuticals Sa filed Critical Glenmark Pharmaceuticals Sa
Publication of MX2016001678A publication Critical patent/MX2016001678A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/468—Immunoglobulins having two or more different antigen binding sites, e.g. multifunctional antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/64—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising a combination of variable region and constant region components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor
    • C12N2840/445—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor for trans-splicing, e.g. polypyrimidine tract, branch point splicing

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

La invención se refiere a un constructo de expresión para la expresión de polipéptidos en células hospederas usando empalme alternativo; el constructor de expresión se puede usar para la expresión de polipéptidos tales como anticuerpos, fragmentos de anticuerpo y anticuerpos biespecíficos por medio de la expresión de productos genéticos que se requieren para la expresión de proteínas en una relación que da lugar a los títulos más altos o el mejor perfil de calidad de producto.
MX2016001678A 2013-08-06 2014-08-05 Construcciones de expresion y metodos para expresar polipeptidos en celulas eucariotas. MX2016001678A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP13179375 2013-08-06
PCT/EP2014/066826 WO2015018832A1 (en) 2013-08-06 2014-08-05 Expression constructs and methods for expressing polypeptides in eukaryotic cells

Publications (1)

Publication Number Publication Date
MX2016001678A true MX2016001678A (es) 2016-10-28

Family

ID=51300736

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016001678A MX2016001678A (es) 2013-08-06 2014-08-05 Construcciones de expresion y metodos para expresar polipeptidos en celulas eucariotas.

Country Status (14)

Country Link
US (3) US20150056655A1 (es)
EP (1) EP3030579A1 (es)
JP (3) JP2016528896A (es)
KR (2) KR20200044154A (es)
CN (1) CN105658665A (es)
AU (2) AU2014304570B2 (es)
BR (1) BR112016002319A2 (es)
CA (1) CA2920574C (es)
EA (1) EA201690271A1 (es)
IL (2) IL243967A0 (es)
MX (1) MX2016001678A (es)
NZ (1) NZ717178A (es)
SG (1) SG11201600736SA (es)
WO (1) WO2015018832A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2997179A1 (en) * 2015-09-02 2017-03-09 The Regents Of The Unversity Of Colorado, A Body Corporate Compositions and methods for modulating t-cell mediated immune response
JP2022509480A (ja) * 2018-10-29 2022-01-20 ユーエムセー・ユトレヒト・ホールディング・ベー・フェー 好中球のＣＤ４７－ＳＩＲＰアルファチェックポイント阻害による異常な細胞のＩｇＡによって媒介される致死
CA3125360A1 (en) 2019-01-01 2020-07-09 Geneneer Ltd. Gene expression elements and systems and use thereof
WO2020205604A1 (en) 2019-03-29 2020-10-08 Salk Institute For Biological Studies High-efficiency reconstitution of rna molecules
GB201919058D0 (en) * 2019-12-20 2020-02-05 Ucb Biopharma Sprl Multi-specific antibodies
CN115417930A (zh) * 2021-12-24 2022-12-02 合肥天港免疫药物有限公司 重组抗体及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6136566A (en) * 1996-10-04 2000-10-24 Lexicon Graphics Incorporated Indexed library of cells containing genomic modifications and methods of making and utilizing the same
US20040072243A1 (en) * 1996-10-11 2004-04-15 Lexicon Genetics Incorporated Indexed library of cells containing genomic modifications and methods of making and utilizing the same
JP2007529223A (ja) * 2004-03-15 2007-10-25 バイオジェン・アイデック・エムエイ・インコーポレイテッド 選択的スプライシングを用いて真核細胞においてポリペプチドマルチマーを発現するための方法および構築物
EP2018430A1 (en) * 2006-05-16 2009-01-28 Millegen Method for expressing polypeptides in eukaryotic cells using alternative splicing
WO2011152043A1 (en) * 2010-06-01 2011-12-08 Kyoto University Transgenic reporter system that reveals expression profiles and regulation mechanisms of alternative splicing in mammalian organisms

Also Published As

Publication number Publication date
AU2019236586A1 (en) 2019-10-10
AU2014304570B2 (en) 2019-07-25
IL243967A0 (en) 2016-04-21
CN105658665A (zh) 2016-06-08
IL269252A (en) 2019-11-28
US20200172634A1 (en) 2020-06-04
US20170253671A1 (en) 2017-09-07
BR112016002319A2 (pt) 2017-09-12
CA2920574C (en) 2021-03-16
AU2019236586B2 (en) 2020-12-03
AU2014304570A1 (en) 2016-03-10
JP2020202840A (ja) 2020-12-24
EA201690271A1 (ru) 2016-07-29
JP2016528896A (ja) 2016-09-23
JP2022177131A (ja) 2022-11-30
KR20200044154A (ko) 2020-04-28
EP3030579A1 (en) 2016-06-15
WO2015018832A1 (en) 2015-02-12
NZ717178A (en) 2022-02-25
US20150056655A1 (en) 2015-02-26
KR20160035084A (ko) 2016-03-30
SG11201600736SA (en) 2016-02-26
CA2920574A1 (en) 2015-02-12
KR102104581B1 (ko) 2020-06-02

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