[go: up one dir, main page]

MX2015010844A - Cultivo liquido de celulas madre epiteliales. - Google Patents

Cultivo liquido de celulas madre epiteliales.

Info

Publication number
MX2015010844A
MX2015010844A MX2015010844A MX2015010844A MX2015010844A MX 2015010844 A MX2015010844 A MX 2015010844A MX 2015010844 A MX2015010844 A MX 2015010844A MX 2015010844 A MX2015010844 A MX 2015010844A MX 2015010844 A MX2015010844 A MX 2015010844A
Authority
MX
Mexico
Prior art keywords
stem cells
epithelial stem
liquid culturing
culturing
liquid
Prior art date
Application number
MX2015010844A
Other languages
English (en)
Inventor
Brian Biehs
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of MX2015010844A publication Critical patent/MX2015010844A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0679—Cells of the gastro-intestinal tract
    • C12N5/068—Stem cells; Progenitors
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/37—Digestive system
    • A61K35/38—Stomach; Intestine; Goblet cells; Oral mucosa; Saliva
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/70—Undefined extracts
    • C12N2500/80—Undefined extracts from animals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/117—Keratinocyte growth factors (KGF-1, i.e. FGF-7; KGF-2, i.e. FGF-12)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/13—Nerve growth factor [NGF]; Brain-derived neurotrophic factor [BDNF]; Cilliary neurotrophic factor [CNTF]; Glial-derived neurotrophic factor [GDNF]; Neurotrophins [NT]; Neuregulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/148—Transforming growth factor alpha [TGF-a]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/42—Notch; Delta; Jagged; Serrate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2503/00—Use of cells in diagnostics
    • C12N2503/02—Drug screening
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Cell Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Virology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Immunology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Physiology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Materials For Medical Uses (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Se proporciona aquí un método para cultivar células madre epiteliales y fragmentos de tejido que comprenden células madre epiteliales en cultivos líquidos.
MX2015010844A 2013-02-25 2014-02-25 Cultivo liquido de celulas madre epiteliales. MX2015010844A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361769076P 2013-02-25 2013-02-25
PCT/US2014/018401 WO2014131033A1 (en) 2013-02-25 2014-02-25 Liquid culturing of epithelial stem cells

Publications (1)

Publication Number Publication Date
MX2015010844A true MX2015010844A (es) 2015-12-03

Family

ID=50240091

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2015010844A MX2015010844A (es) 2013-02-25 2014-02-25 Cultivo liquido de celulas madre epiteliales.

Country Status (11)

Country Link
US (1) US20150361392A1 (es)
EP (1) EP2958991A1 (es)
JP (1) JP2016512958A (es)
KR (1) KR20150123805A (es)
CN (1) CN105026552A (es)
BR (1) BR112015017977A8 (es)
CA (1) CA2900561A1 (es)
HK (1) HK1216904A1 (es)
MX (1) MX2015010844A (es)
RU (1) RU2015140912A (es)
WO (1) WO2014131033A1 (es)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111334463B (zh) * 2016-05-18 2024-02-20 学校法人庆应义塾 类器官培养用细胞培养基、培养方法及类器官
EP3505620B1 (en) * 2016-08-24 2023-07-26 Keio University 2d organoid for infection and culture of human diarrhea virus, and use of said 2d organoid
CN110382689A (zh) 2017-02-20 2019-10-25 公立大学法人名古屋市立大学 来自人工多能性干细胞的肠道干细胞的维持培养
CN109055304B (zh) * 2018-08-16 2021-12-07 洪玥 一种非柱状上皮干细胞培养基及培养方法
JP7308611B2 (ja) * 2018-12-07 2023-07-14 関東化学株式会社 多能性幹細胞用未分化維持培地
US12221630B2 (en) 2019-02-19 2025-02-11 Miltenyi Biotec B.V. & Co. KG Cell culture medium and method for generation of epithelial organoids from epithelial stem cells
WO2021113924A1 (en) * 2019-12-12 2021-06-17 The Walter And Eliza Hall Institute Of Medical Research Organoid cultures
US20220339254A1 (en) * 2021-04-27 2022-10-27 Avita Medical, Inc. Regenerative bioactive suspension derived from freshly disaggregated tissue and methods of use in clinical therapies
CN116396939B (zh) * 2023-05-11 2024-07-19 杭州济扶科技有限公司 一种适应泛癌种类器官高效扩增的组合式培养基及应用

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6331406B1 (en) * 1997-03-31 2001-12-18 The John Hopkins University School Of Medicine Human enbryonic germ cell and methods of use
CA2388208A1 (en) * 1999-10-29 2001-05-10 Mcgill University Medium for preparing dedifferentiated cells
JP2003009854A (ja) * 2001-04-09 2003-01-14 Kyowa Hakko Kogyo Co Ltd エンブリオイドボディ形成方法及びその用途
AU2004288722A1 (en) * 2003-11-04 2005-05-26 U.S. Department Of Veterans Affairs Stem cell culture medium and method of using said medium and the cells
JP2008099662A (ja) * 2006-09-22 2008-05-01 Institute Of Physical & Chemical Research 幹細胞の培養方法
US10227563B2 (en) * 2008-06-06 2019-03-12 Riken Method for culture of stem cell
JP5490803B2 (ja) 2008-09-22 2014-05-14 ウニヴェルジテート チューリッヒ プロレクトラート エムエヌヴェー 懸滴プレートおよび同懸滴プレートを使用する方法
GB201111244D0 (en) * 2011-06-30 2011-08-17 Konink Nl Akademie Van Wetenschappen Knaw Culture media for stem cells
US9752124B2 (en) * 2009-02-03 2017-09-05 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium for epithelial stem cells and organoids comprising the stem cells
MX379945B (es) * 2009-02-03 2025-03-11 Koninklijke Nederlandse Akademie Van Wetenschappen Medio de cultivo para celulas madre epiteliales y organoides que comprenden las celulas madre.
WO2013013826A1 (en) * 2011-07-27 2013-01-31 Friedrich-Alexander-Universität Erlangen-Nürnberg Necroptosis inhibitors for the treatment of inflammatory diseases of the gastrointestinal tract

Also Published As

Publication number Publication date
BR112015017977A2 (pt) 2017-07-11
BR112015017977A8 (pt) 2019-11-05
JP2016512958A (ja) 2016-05-12
RU2015140912A (ru) 2017-03-30
CA2900561A1 (en) 2014-08-28
KR20150123805A (ko) 2015-11-04
HK1216904A1 (zh) 2016-12-09
EP2958991A1 (en) 2015-12-30
CN105026552A (zh) 2015-11-04
WO2014131033A1 (en) 2014-08-28
US20150361392A1 (en) 2015-12-17

Similar Documents

Publication Publication Date Title
MX2015010844A (es) Cultivo liquido de celulas madre epiteliales.
ZA201904258B (en) Compositions and methods for epithelial stem cell expansion and culture
IN2015KN00666A (es)
MX2015012202A (es) Metodos y materiales para diferenciacion hematoendotelial de celulas germinales pluripotentes humanas bajo condiciones definidas.
EP3047017A4 (en) Methods of mammalian retinal stem cell production and applications
SG11201508387WA (en) Polycaprolactone microcarriers for stem cell culture and fabrication thereof
IL237530A0 (en) Gene and protein expression characteristics of adherent stromal cells grown in 3D
EP2970894A4 (en) Stem cell culture media and methods of enhancing cell survival
EP2670386A1 (en) New use of an extract of plant origin of globularia and method for obtaining said extract by in vitro plant culture
AU338747S (en) Water bottle
IL243634A0 (en) Method and medium for in vitro growth of stem cells
CA3279033A1 (en) Methods of using human mesenchymal stem cells to effect cellular and humoral immunity
AU342936S (en) Insole
AU340528S (en) Insole
SG11201507828VA (en) Method for culturing hepatoblast-like cells and culture product thereof
SG11201503633VA (en) Methods of culturing cells or tissues and devices for cell or tissue culture
MY166042A (en) Lactobacillus culture and method of producing same
SG11201510462QA (en) Method of culturing cancer stem cells
SG11201913778VA (en) Stem cell-derived skin precursor cell culture medium and preparation method therefor
EP3045531A4 (en) Method for inducing differentiation of induced pluripotent stem cells and method for selecting induced pluripotent stem cells
HK40014250A (en) Methods of using human mesenchymal stem cells to effect cellular and humoral immunity
GB201307377D0 (en) Devices and methods for culture of cells
AU339336S (en) Walking frames for disabled
AU340418S (en) An adjustable arm for container-handling equipment
AU340049S (en) Upside down keg