MX2015004373A - Nucleic acid molecules for increased protein production. - Google Patents
Nucleic acid molecules for increased protein production.Info
- Publication number
- MX2015004373A MX2015004373A MX2015004373A MX2015004373A MX2015004373A MX 2015004373 A MX2015004373 A MX 2015004373A MX 2015004373 A MX2015004373 A MX 2015004373A MX 2015004373 A MX2015004373 A MX 2015004373A MX 2015004373 A MX2015004373 A MX 2015004373A
- Authority
- MX
- Mexico
- Prior art keywords
- mutated
- increased protein
- nucleic acid
- acid molecules
- signal peptide
- Prior art date
Links
- 108020004707 nucleic acids Proteins 0.000 title abstract 3
- 102000039446 nucleic acids Human genes 0.000 title abstract 3
- 150000007523 nucleic acids Chemical class 0.000 title abstract 3
- 230000014616 translation Effects 0.000 title abstract 2
- 108010076504 Protein Sorting Signals Proteins 0.000 abstract 4
- 108090000623 proteins and genes Proteins 0.000 abstract 4
- 102000004169 proteins and genes Human genes 0.000 abstract 4
- 239000013604 expression vector Substances 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 102000004882 Lipase Human genes 0.000 abstract 1
- 108090001060 Lipase Proteins 0.000 abstract 1
- 239000004367 Lipase Substances 0.000 abstract 1
- 235000019421 lipase Nutrition 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 230000028327 secretion Effects 0.000 abstract 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to the improved production of proteins, preferably enzymes such as lipases. In particular, the invention relates to a mutated signal peptide and nucleotide sequence encoding said signal peptide that results in an increased protein secretion. Further, the invention relates to a mutated promoter that results in an increased protein expression. It was surprisingly found that the combination of the mutated signal peptide and the mutated promoter act synergistically to result in an about 100-fold increased protein production. Nucleic acid molecules, expression vectors and host cells comprising the mutated signal peptide, mutated promoter, or the combination thereof are also encompassed by the invention. Finally, the invention relates to methods and uses of such nucleic acid molecules, expression vectors and host cells for protein preparation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MX2015004373A MX2015004373A (en) | 2015-04-07 | 2015-04-07 | Nucleic acid molecules for increased protein production. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MX2015004373A MX2015004373A (en) | 2015-04-07 | 2015-04-07 | Nucleic acid molecules for increased protein production. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2015004373A true MX2015004373A (en) | 2016-10-06 |
Family
ID=58161142
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2015004373A MX2015004373A (en) | 2015-04-07 | 2015-04-07 | Nucleic acid molecules for increased protein production. |
Country Status (1)
| Country | Link |
|---|---|
| MX (1) | MX2015004373A (en) |
-
2015
- 2015-04-07 MX MX2015004373A patent/MX2015004373A/en unknown
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