[go: up one dir, main page]

MX2014015842A - Purificacion de particulas tipo virus. - Google Patents

Purificacion de particulas tipo virus.

Info

Publication number
MX2014015842A
MX2014015842A MX2014015842A MX2014015842A MX2014015842A MX 2014015842 A MX2014015842 A MX 2014015842A MX 2014015842 A MX2014015842 A MX 2014015842A MX 2014015842 A MX2014015842 A MX 2014015842A MX 2014015842 A MX2014015842 A MX 2014015842A
Authority
MX
Mexico
Prior art keywords
particles
virus
purification
methods
vlps
Prior art date
Application number
MX2014015842A
Other languages
English (en)
Other versions
MX366234B (es
Inventor
Ross Taylor
Original Assignee
Takeda Vaccines Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takeda Vaccines Inc filed Critical Takeda Vaccines Inc
Publication of MX2014015842A publication Critical patent/MX2014015842A/es
Publication of MX366234B publication Critical patent/MX366234B/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12Antivirals
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12Antivirals
    • A61P31/20Antivirals for DNA viruses
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011Details
    • C12N2710/14011Baculoviridae
    • C12N2710/14111Nucleopolyhedrovirus, e.g. autographa californica nucleopolyhedrovirus
    • C12N2710/14141Use of virus, viral particle or viral elements as a vector
    • C12N2710/14143Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011Details
    • C12N2770/16011Caliciviridae
    • C12N2770/16022New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011Details
    • C12N2770/16011Caliciviridae
    • C12N2770/16023Virus like particles [VLP]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011Details
    • C12N2770/16011Caliciviridae
    • C12N2770/16051Methods of production or purification of viral material

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Virology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Analytical Chemistry (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

Métodos de purificación de partículas tipo virus (VLP), sustancialmente libres de contaminantes de proceso y agentes infecciosos. Los métodos incorporan, por ejemplo, un tratamiento a pH bajo durante la cosecha y/o inactivación con solvente y/o detergente durante la captura de las VLP.
MX2014015842A 2012-06-22 2013-06-24 Purificacion de particulas tipo virus. MX366234B (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261663218P 2012-06-22 2012-06-22
US201361794086P 2013-03-15 2013-03-15
PCT/US2013/047249 WO2013192604A1 (en) 2012-06-22 2013-06-24 Purification of virus like particles

Publications (2)

Publication Number Publication Date
MX2014015842A true MX2014015842A (es) 2015-09-07
MX366234B MX366234B (es) 2019-07-03

Family

ID=49769461

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014015842A MX366234B (es) 2012-06-22 2013-06-24 Purificacion de particulas tipo virus.

Country Status (15)

Country Link
US (3) US10167320B2 (es)
EP (1) EP2864478B1 (es)
JP (1) JP6553509B2 (es)
KR (1) KR102110876B1 (es)
CN (2) CN104583393A (es)
AR (1) AR091538A1 (es)
AU (1) AU2013277959B2 (es)
CA (1) CA2879815A1 (es)
IN (1) IN2015DN00089A (es)
JO (1) JOP20130186B1 (es)
MX (1) MX366234B (es)
SG (2) SG10201610753YA (es)
TW (1) TWI647310B (es)
UY (1) UY34875A (es)
WO (1) WO2013192604A1 (es)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PL2601970T3 (pl) 2006-09-29 2017-07-31 Takeda Vaccines, Inc. Preparaty szczepionki przeciw norowirusowi
AU2008224877B2 (en) 2007-03-14 2013-07-11 Ligocyte Pharmaceuticals, Inc. Virus like particle purification
SG10201601660YA (en) 2007-09-18 2016-04-28 Takeda Vaccines Inc Method of conferring a protective immune response to norovirus
MX356586B (es) 2011-07-11 2018-06-05 Takeda Vaccines Inc Formulaciones parenterales de vacunas contra los norovirus.
JOP20130186B1 (ar) 2012-06-22 2021-08-17 Takeda Vaccines Montana Inc تنقية الجزيئات الشبيهة بالفيروسات
CN105339495A (zh) * 2013-07-11 2016-02-17 宝生物工程株式会社 制备无包膜病毒的方法
CA2926225A1 (en) 2013-10-03 2015-04-09 Takeda Vaccines, Inc. Methods of detection and removal of rhabdoviruses from cell lines
CN103981084B (zh) * 2014-05-29 2015-11-18 黑龙江省科学院微生物研究所 水中病毒分离提取装置及水中札幌病毒的提取方法
JP6616329B2 (ja) * 2015-01-09 2019-12-04 タカラバイオ株式会社 非エンベロープウイルス粒子の製造方法
JP6893174B2 (ja) * 2015-01-16 2021-06-23 タケダ ワクチン,インコーポレイテッド 粒子に含まれる逆転写酵素活性の検出
CN105907729B (zh) * 2015-05-05 2019-08-16 广东东阳光药业有限公司 一种制备人乳头瘤病毒l1蛋白类病毒样颗粒的方法
CN107034198B (zh) * 2015-07-15 2021-03-09 长春百克生物科技股份公司 一种嵌合诺如病毒p颗粒及其制备和应用
AU2016317661B2 (en) * 2015-08-28 2023-05-18 Ology Bioservices, Inc. Norovirus vaccine
CN105779399A (zh) * 2016-03-28 2016-07-20 广西壮族自治区林业科学研究院 一种昆虫杆状病毒纯化方法
GB201614050D0 (en) * 2016-08-17 2016-09-28 Glaxosmithkline Ip Dev Ltd Method for purifying viral vectors
BR112019004187A2 (pt) * 2016-09-01 2019-06-25 Takeda Pharmaceuticals Co método para produzir um vírus enterovírus c, e, vírus enterovírus c.
EP3420076B1 (en) * 2017-03-06 2024-02-21 Guangzhou Realbenefitspot Pharmaceutical Co., Ltd. Methods of producing and characterizing virus vaccine and virus vaccine composition
ES2975182T3 (es) 2017-03-06 2024-07-03 Guangzhou Realbenefitspot Pharmaceutical Co Ltd Métodos de producción y caracterización de vacunas víricas y composición de vacuna vírica
JP2021514391A (ja) 2018-02-15 2021-06-10 アイコン ジェネティクス ゲーエムベーハー ノロウイルスに対する免疫応答を生成するための免疫原性組成物及びワクチン
CN108676781A (zh) * 2018-05-18 2018-10-19 山东信得动物疫苗有限公司 一种重组禽流感病毒的工业化纯化方法
CN111848749A (zh) * 2018-05-23 2020-10-30 中国农业科学院兰州兽医研究所 猪细小病毒VLPs抗体检测试剂盒及其制备方法、应用
ES2953232T3 (es) 2019-03-12 2023-11-08 Icon Genetics Gmbh Partículas similares a norovirus con estabilidad mejorada
WO2021067389A1 (en) 2019-09-30 2021-04-08 Bioverativ Therapeutics Inc. Lentiviral vector formulations
JP2024519800A (ja) 2021-05-21 2024-05-21 タケダ ワクチン,インコーポレイテッド 固体組成物、フリーズドライ方法およびガラスバイアル
CA3224156A1 (en) * 2021-06-28 2023-01-05 VST LLC dba Medgene Labs Sapovirus vaccines
CN116789773B (zh) * 2022-11-30 2024-02-20 华北制药金坦生物技术股份有限公司 一种胞内表达病毒样颗粒的粗纯方法
WO2025077800A1 (en) * 2023-10-12 2025-04-17 Chengdu Kanghua Biological Products Co., Ltd. Purification of norovirus virus-like particles (vlps)
CN118126202B (zh) * 2024-05-07 2024-07-30 上海瑞宙生物科技有限公司 一种诺如病毒类病毒颗粒及其应用

Family Cites Families (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2833106B2 (ja) 1990-02-21 1998-12-09 株式会社島津製作所 液体クロマトグラフ
WO1994026087A2 (en) 1993-05-14 1994-11-24 Connor Kim C O Recombinant protein production and insect cell culture and process
AU693203B2 (en) 1994-05-16 1998-06-25 Merck Sharp & Dohme Corp. Papillomavirus vaccines
AR004464A1 (es) 1994-11-14 1998-12-16 Merck Sharp & Dohme Un metodo para producir una proteina de capside de papilomavirus
JP3316325B2 (ja) * 1994-12-20 2002-08-19 富士シリシア化学株式会社 ビール安定化処理用シリカゲル及びその製造方法並びにビールの安定化処理方法
US5641870A (en) 1995-04-20 1997-06-24 Genentech, Inc. Low pH hydrophobic interaction chromatography for antibody purification
DE69739268D1 (de) 1996-11-08 2009-04-02 Us Gov Health & Human Serv Herstellung und reinigung von hepatitis c virus-ähnlichen partikeln
ES2268787T3 (es) 1997-09-05 2007-03-16 Medimmune, Inc. Metodo in vitro de desmontaje/embalaje de particulas similares a virus (vlp) de papilomavirus.
DE19823814A1 (de) 1998-05-27 1999-12-02 Octapharma Ag Ziegelbruecke Verfahren zur Trennung und/oder Isolierung von Plasmaproteinen mit annularer Chromatographie
PL347472A1 (en) 1998-08-14 2002-04-08 Merck & Co Inc Process for purifying human papillomavirus virus-like particles
DE60040727D1 (de) * 1999-02-05 2008-12-18 Merck & Co Inc Menschliche papillomavirus impfstoff-formulierungen
US6349843B1 (en) 1999-04-01 2002-02-26 The Vollrath Company Llc Pan removal ramp
KR100762092B1 (ko) 1999-06-22 2007-10-04 국립감염증연구소장이 대표하는 일본국 Srsv 검출 킷트
DE10012732A1 (de) 2000-03-18 2001-09-20 Aventis Behring Gmbh Thrombin-Zubereitungen und Verfahren zu ihrer Herstellung
JP2003066021A (ja) 2001-08-28 2003-03-05 Hiroshi Shionoya 連続分取液体クロマトグラフィー装置とそれを用いる方法
AU2003219760A1 (en) 2002-02-14 2003-09-04 Novavax, Inc. Optimization of gene sequences of virus-like particles for expression in insect cells
US20060234226A1 (en) 2002-04-26 2006-10-19 Fahner Robert L Non-affinity purification of proteins
US20040106184A1 (en) 2002-08-28 2004-06-03 Introgen Therapeutics Inc. Chromatographic methods for adenovirus purification
US7879338B2 (en) 2003-07-21 2011-02-01 Boyce Thompson Institute For Plant Research Vectors and methods for immunization against norovirus using transgenic plants
US7481997B1 (en) 2004-02-12 2009-01-27 Montana State University Snow mountain virus genome sequence, virus-like particles and methods of use
US8277819B2 (en) 2005-06-16 2012-10-02 Children's Hospital Medical Center Norovirus particle for use as an antiviral or vaccine
EP1736538A1 (en) 2005-06-21 2006-12-27 Cytos Biotechnology AG Process for the preparative purification of virus-like-particles (VLPs)
EP2360175B1 (en) 2005-11-22 2014-07-16 Novartis Vaccines and Diagnostics, Inc. Norovirus and Sapovirus virus-like particles (VLPs)
BR122015028489B1 (pt) 2005-12-29 2021-10-13 Boehringer Ingelheim Animal Health Usa Inc Vacina de combinação multivalente contra pcv2, bem como uso de uma proteína orf2 na preparação da mesma
AU2007345768B2 (en) 2006-07-27 2013-08-01 Ligocyte Pharmaceuticals, Inc. Chimeric influenza virus-like particles
PL2601970T3 (pl) 2006-09-29 2017-07-31 Takeda Vaccines, Inc. Preparaty szczepionki przeciw norowirusowi
US8202967B2 (en) 2006-10-27 2012-06-19 Boehringer Ingelheim Vetmedica, Inc. H5 proteins, nucleic acid molecules and vectors encoding for those, and their medicinal use
AU2008224877B2 (en) * 2007-03-14 2013-07-11 Ligocyte Pharmaceuticals, Inc. Virus like particle purification
EP2027875A1 (en) 2007-08-23 2009-02-25 Octapharma AG A Process for Isolation and Purification of a Target Protein free of Prion Protein (PrPSC)
SG10201601660YA (en) 2007-09-18 2016-04-28 Takeda Vaccines Inc Method of conferring a protective immune response to norovirus
EP2235165A1 (en) * 2007-12-21 2010-10-06 Cytos Biotechnology AG Process for clarifying cell homogenates
KR102118458B1 (ko) 2008-08-08 2020-06-03 다케다 백신즈 인코포레이티드 강화된 교차 반응성을 가진 복합체 캡시드 아미노산 서열들을 포함하는 바이러스-유사 입자들
WO2010062757A1 (en) 2008-11-03 2010-06-03 Ligocyte Pharmaceuticals, Inc. Improved methods for isolating enveloped virus-based vlps free of infectious agents
US8945895B2 (en) * 2009-07-31 2015-02-03 Baxter International Inc. Methods of purifying recombinant ADAMTS13 and other proteins and compositions thereof
AU2011207355B2 (en) 2010-01-21 2015-04-02 Takeda Vaccines, Inc. Targeted heterologous antigen presentation on Calicivirus virus-like particles
EP2619217B1 (en) 2010-09-20 2016-11-09 Bio-Rad Laboratories, Inc. Dissociation of product-complexed contaminants in chromatography
FI122520B (fi) 2010-10-15 2012-03-15 Vesna Blazevic Noroviruksen kapsidi ja rotaviruksen VP6-proteiini käytettäväksi yhdistelmärokotteena
CN102260651A (zh) * 2011-06-24 2011-11-30 华中科技大学同济医学院附属协和医院 一种纯化病毒样颗粒蛋白的方法及其应用
JOP20130186B1 (ar) 2012-06-22 2021-08-17 Takeda Vaccines Montana Inc تنقية الجزيئات الشبيهة بالفيروسات
JP2015015931A (ja) 2013-07-12 2015-01-29 株式会社Umnファーマ ウイルス様粒子を含む培養物の製造方法

Also Published As

Publication number Publication date
KR102110876B1 (ko) 2020-05-14
CN114085273B (zh) 2024-07-12
US11091519B2 (en) 2021-08-17
IN2015DN00089A (es) 2015-05-29
AU2013277959B2 (en) 2019-05-16
AR091538A1 (es) 2015-02-11
CA2879815A1 (en) 2013-12-27
SG11201408459VA (en) 2015-01-29
UY34875A (es) 2013-12-31
CN104583393A (zh) 2015-04-29
TW201406956A (zh) 2014-02-16
US10167320B2 (en) 2019-01-01
US20140004145A1 (en) 2014-01-02
HK1210210A1 (en) 2016-04-15
AU2013277959A1 (en) 2015-01-22
US20190169236A1 (en) 2019-06-06
US20220213147A1 (en) 2022-07-07
KR20150051988A (ko) 2015-05-13
WO2013192604A1 (en) 2013-12-27
SG10201610753YA (en) 2017-02-27
EP2864478A1 (en) 2015-04-29
EP2864478A4 (en) 2015-12-02
US12286456B2 (en) 2025-04-29
JP6553509B2 (ja) 2019-07-31
MX366234B (es) 2019-07-03
TWI647310B (zh) 2019-01-11
JOP20130186B1 (ar) 2021-08-17
EP2864478B1 (en) 2020-12-09
JP2015521628A (ja) 2015-07-30
CN114085273A (zh) 2022-02-25

Similar Documents

Publication Publication Date Title
MX2014015842A (es) Purificacion de particulas tipo virus.
PH12015501017A1 (en) Alternative uses for hbv assembly effectors
EA201790630A1 (ru) Способы получения рибозидов
PH12017501258A1 (en) Pyrazine compounds for the treatment of infectious diseases
EA030115B9 (ru) Новые 6-конденсированные гетероарилдигидропиримидины для лечения и профилактики заражения вирусом гепатита b
EA201391152A1 (ru) Ингибиторы вируса гепатита с
SG11201406407TA (en) Methods for inactivating viruses during a protein purification process
EA026667B9 (ru) Фармацевтическая композиция для лечения вируса гепатита с
EA201691261A1 (ru) Новые дигидрохинолизиноны для лечения и профилактики инфекции, вызванной вирусом гепатита b
MY161252A (en) Viral inactivation using improved solvent-detergent method
EA201370017A1 (ru) Ингибиторы вируса гепатита с
PH12013502442A1 (en) Inactivated dengue virus vaccine
PH12013502177A1 (en) Hepatitis c virus inhibitors
EA201692034A1 (ru) Новые макроциклические соединения
WO2012006149A3 (en) Analogs of c5a and methods of using same
ZA201401020B (en) Benzofuran compounds for the treatment of hepatitis c virus infections
IL232393A0 (en) Substituted benzylamine compounds, their use in medicine, and in particular the treatment of hepatitis c virus (hcv) infection
EP2731507A4 (en) IMPLANTS, TOOLS, AND METHODS FOR TREATING PELVIC DISEASES
DK2739725T3 (da) Fremgangsmåde til oprensning af virus-lignende partiker (vlp)
BR112017006589A2 (pt) um método de purificação de lignina por sujeição de uma pasta fluida compreendendo lignina para um campo elétrico
WO2013026452A9 (en) Method for removing immunosuppresive properties of hiv envelope glycoproteins
MX354758B (es) Metodos para la liberacion de las particulas similares a virus.
WO2014008475A3 (en) Compositions and methods related to viral vaccines
GB201008123D0 (en) Novel compounds
EA201691777A1 (ru) Содержащие цианогруппу соединения азабензофурана для лечения гепатита с

Legal Events

Date Code Title Description
FG Grant or registration