[go: up one dir, main page]

MX2013012925A - Metodos y composiciones para silenciar familias de genes mediante el uso de microarn artificiales. - Google Patents

Metodos y composiciones para silenciar familias de genes mediante el uso de microarn artificiales.

Info

Publication number
MX2013012925A
MX2013012925A MX2013012925A MX2013012925A MX2013012925A MX 2013012925 A MX2013012925 A MX 2013012925A MX 2013012925 A MX2013012925 A MX 2013012925A MX 2013012925 A MX2013012925 A MX 2013012925A MX 2013012925 A MX2013012925 A MX 2013012925A
Authority
MX
Mexico
Prior art keywords
mirna
expression
gene family
methods
compositions
Prior art date
Application number
MX2013012925A
Other languages
English (en)
Inventor
Brian Mcgonigle
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of MX2013012925A publication Critical patent/MX2013012925A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8247—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified lipid metabolism, e.g. seed oil composition

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Virology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Se proporcionan métodos y composiciones que permiten que un solo microARN (miARN) reduzca el nivel de expresión de al menos dos miembros de la misma familia de genes y/o proteínas. Si bien un solo miARN de 21 pares de bases puede causar el clivaje de diversas especies de ARNm, una familia completa de genes no se puede silenciar a menos que esa familia de genes comparta una identidad casi completa dentro de una región de 21 pares de bases que, además, se puede clivar por medio de un solo miARN. En ciertas modalidades, todos los miembros de una familia de proteínas y/o genes determinados se pueden suprimir con un constructo de expresión de miARN descrito en la presente descripción aun si no comparten una alta identidad dentro de una región de 21 pares de bases que, además, puede funcionar como un miARN. Tales métodos y composiciones emplean constructos de expresión de miARN que tienen una estructura tal que la forma más abundante de miARN producida a partir del constructo es un miARN de 22 nucleótidos. Por lo tanto, el miARN de 22 nucleótidos producido a partir del constructo de expresión de miARN reduce el nivel de expresión de la secuencia objetivo para el miARN y, además, reduce el nivel de expresión de al menos una secuencia adicional de la misma familia de proteínas y/o genes como la secuencia objetivo.
MX2013012925A 2011-05-09 2012-05-09 Metodos y composiciones para silenciar familias de genes mediante el uso de microarn artificiales. MX2013012925A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161484033P 2011-05-09 2011-05-09
PCT/US2012/037083 WO2012154824A1 (en) 2011-05-09 2012-05-09 Methods and compositions for silencing gene families using artificial micrornas

Publications (1)

Publication Number Publication Date
MX2013012925A true MX2013012925A (es) 2013-11-21

Family

ID=46210414

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2013012925A MX2013012925A (es) 2011-05-09 2012-05-09 Metodos y composiciones para silenciar familias de genes mediante el uso de microarn artificiales.

Country Status (10)

Country Link
US (2) US9062317B2 (es)
EP (1) EP2707484A1 (es)
CN (1) CN103547673A (es)
AU (1) AU2012253534A1 (es)
BR (1) BR112013028839A2 (es)
CA (1) CA2832639A1 (es)
MX (1) MX2013012925A (es)
RU (1) RU2013154399A (es)
WO (1) WO2012154824A1 (es)
ZA (1) ZA201306745B (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20160024518A1 (en) 2013-03-13 2016-01-28 Brian McGonigle Production of small interfering rnas in planta
EP3842535A1 (en) 2014-04-30 2021-06-30 Fondazione Edmund Mach Plant srna extract or plant mirna for use as immunosuppressive agent
CN106448353B (zh) * 2016-10-18 2023-07-07 电子科技大学 基因工程演示模型

Family Cites Families (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4945050A (en) 1984-11-13 1990-07-31 Cornell Research Foundation, Inc. Method for transporting substances into living cells and tissues and apparatus therefor
US5569597A (en) 1985-05-13 1996-10-29 Ciba Geigy Corp. Methods of inserting viral DNA into plant material
US5268463A (en) 1986-11-11 1993-12-07 Jefferson Richard A Plant promoter α-glucuronidase gene construct
US5608142A (en) 1986-12-03 1997-03-04 Agracetus, Inc. Insecticidal cotton plants
US5316931A (en) 1988-02-26 1994-05-31 Biosource Genetics Corp. Plant viral vectors having heterologous subgenomic promoters for systemic expression of foreign genes
US5990387A (en) 1988-06-10 1999-11-23 Pioneer Hi-Bred International, Inc. Stable transformation of plant cells
US5023179A (en) 1988-11-14 1991-06-11 Eric Lam Promoter enhancer element for gene expression in plant roots
US5110732A (en) 1989-03-14 1992-05-05 The Rockefeller University Selective gene expression in plants
HU218717B (hu) 1989-03-17 2000-11-28 E. I. Du Pont De Nemours And Co. Nukleinsav-termelést fokozó növényi eredetű génfragmentek és eljárás előállításukra
US5879918A (en) 1989-05-12 1999-03-09 Pioneer Hi-Bred International, Inc. Pretreatment of microprojectiles prior to using in a particle gun
US5240855A (en) 1989-05-12 1993-08-31 Pioneer Hi-Bred International, Inc. Particle gun
US5322783A (en) 1989-10-17 1994-06-21 Pioneer Hi-Bred International, Inc. Soybean transformation by microparticle bombardment
EP0452269B1 (en) 1990-04-12 2002-10-09 Syngenta Participations AG Tissue-preferential promoters
US5498830A (en) 1990-06-18 1996-03-12 Monsanto Company Decreased oil content in plant seeds
US5932782A (en) 1990-11-14 1999-08-03 Pioneer Hi-Bred International, Inc. Plant transformation method using agrobacterium species adhered to microprojectiles
US5459252A (en) 1991-01-31 1995-10-17 North Carolina State University Root specific gene promoter
US5399680A (en) 1991-05-22 1995-03-21 The Salk Institute For Biological Studies Rice chitinase promoter
EP0604458A1 (en) 1991-07-24 1994-07-06 E.I. Du Pont De Nemours And Company Nucleotide sequences of galactinol synthase from zucchini and soybean
ATE186571T1 (de) 1991-08-27 1999-11-15 Novartis Ag Proteine mit insektiziden eigenschaften gegen homopteran insekten und ihre verwendung im pflanzenschutz
ZA927576B (en) 1991-10-04 1993-04-16 Univ North Carolina State Pathogen-resistant transgenic plants.
US5324646A (en) 1992-01-06 1994-06-28 Pioneer Hi-Bred International, Inc. Methods of regeneration of Medicago sativa and expressing foreign DNA in same
US5401836A (en) 1992-07-16 1995-03-28 Pioneer Hi-Bre International, Inc. Brassica regulatory sequence for root-specific or root-abundant gene expression
EP0652965A1 (en) 1992-07-27 1995-05-17 Pioneer Hi-Bred International, Inc. An improved method of agrobacterium-mediated transformation of cultured soybean cells
AU5407594A (en) 1992-11-17 1994-06-08 E.I. Du Pont De Nemours And Company Genes for microsomal delta-12 fatty acid desaturases and related enzymes from plants
IL108241A (en) 1992-12-30 2000-08-13 Biosource Genetics Corp Plant expression system comprising a defective tobamovirus replicon integrated into the plant chromosome and a helper virus
US5814618A (en) 1993-06-14 1998-09-29 Basf Aktiengesellschaft Methods for regulating gene expression
US5789156A (en) 1993-06-14 1998-08-04 Basf Ag Tetracycline-regulated transcriptional inhibitors
US5470353A (en) 1993-10-20 1995-11-28 Hollister Incorporated Post-operative thermal blanket
US5633363A (en) 1994-06-03 1997-05-27 Iowa State University, Research Foundation In Root preferential promoter
US5736369A (en) 1994-07-29 1998-04-07 Pioneer Hi-Bred International, Inc. Method for producing transgenic cereal plants
US5608144A (en) 1994-08-12 1997-03-04 Dna Plant Technology Corp. Plant group 2 promoters and uses thereof
US5750868A (en) 1994-12-08 1998-05-12 Pioneer Hi-Bred International, Inc. Reversible nuclear genetic system for male sterility in transgenic plants
US5837876A (en) 1995-07-28 1998-11-17 North Carolina State University Root cortex specific gene promoter
US6072050A (en) 1996-06-11 2000-06-06 Pioneer Hi-Bred International, Inc. Synthetic promoters
US5981840A (en) 1997-01-24 1999-11-09 Pioneer Hi-Bred International, Inc. Methods for agrobacterium-mediated transformation
AU7564898A (en) 1997-05-07 1998-11-27 E.I. Du Pont De Nemours And Company Starch biosynthetic enzymes
AU757672B2 (en) 1997-11-18 2003-02-27 Pioneer Hi-Bred International, Inc. A method for directional stable transformation of eukaryotic cells
EP1034286B1 (en) 1997-11-18 2010-01-06 Pioneer Hi-Bred International, Inc. Mobilization of viral genomes from t-dna using site-specific recombination systems
ATE317440T1 (de) 1997-11-18 2006-02-15 Pioneer Hi Bred Int Neuartige methode zur integration von fremd-dna ins eukaryotische genom
AU1526199A (en) 1997-11-18 1999-06-07 Pioneer Hi-Bred International, Inc. Targeted manipulation of herbicide-resistance genes in plants
DE69932868T2 (de) 1998-02-26 2007-03-15 Pioneer Hi-Bred International, Inc. Nukleinsäuremolekül mit einer Nukleotidsequenz für einen Promotor
WO2000011177A1 (en) 1998-08-20 2000-03-02 Pioneer Hi-Bred International, Inc. Seed-preferred promoters
WO2000012733A1 (en) 1998-08-28 2000-03-09 Pioneer Hi-Bred International, Inc. Seed-preferred promoters from end genes
AU768243B2 (en) 1998-11-09 2003-12-04 E.I. Du Pont De Nemours And Company Transcriptional activator LEC1 nucleic acids, polypeptides and their uses
WO2001077306A2 (en) 2000-04-11 2001-10-18 E. I. Du Pont De Nemours And Company Plant raffinose saccharide biosynthetic enzymes
WO2002000904A2 (en) 2000-06-23 2002-01-03 E. I. Du Pont De Nemours And Company Recombinant constructs and their use in reducing gene expression
US7294756B2 (en) * 2004-06-22 2007-11-13 E.I. Du Pont De Nemours And Company Plant galactinol synthase homologs
EP1874935B2 (en) 2005-04-04 2023-10-18 E. I. du Pont de Nemours and Company Polynucleotides and methods for making plants resistant to fungal pathogens
US20070044164A1 (en) * 2005-05-31 2007-02-22 Cold Spring Harbor Laboratory Methods for producing microRNAs
US8115055B2 (en) 2007-12-18 2012-02-14 E.I. Du Pont De Nemours And Company Down-regulation of gene expression using artificial microRNAs
US8937217B2 (en) * 2007-12-18 2015-01-20 E. I. Du Pont De Nemours And Company Down-regulation of gene expression using artificial microRNAs

Also Published As

Publication number Publication date
US20150252376A1 (en) 2015-09-10
US20120297508A1 (en) 2012-11-22
WO2012154824A1 (en) 2012-11-15
CA2832639A1 (en) 2012-11-15
US9719102B2 (en) 2017-08-01
RU2013154399A (ru) 2015-06-20
AU2012253534A1 (en) 2013-09-26
US9062317B2 (en) 2015-06-23
BR112013028839A2 (pt) 2017-01-31
CN103547673A (zh) 2014-01-29
ZA201306745B (en) 2014-11-26
EP2707484A1 (en) 2014-03-19

Similar Documents

Publication Publication Date Title
EA201171018A1 (ru) Биореактор для культивирования клеток млекопитающих
MX2020011807A (es) Composiciones y metodos para inhibir la expresion genica del virus de hepatitis b.
PH12017501501A1 (en) Compositions and methods for inhibiting expression of tmprss6 gene
PH12015501953A1 (en) Substituted nucleotide analogs
EA201692359A1 (ru) Композиция на основе нового штамма prrsv или его белков, продукт, их применение, вирус prrsv, выделенная нк и рекомбинантный вектор экспрессии для получения этого вируса
WO2016061232A3 (en) Multiplexed shrnas and uses thereof
BR112015018493A2 (pt) Linhagens celulares para produção de vírus e métodos de uso
AU2007240448A8 (en) Methods and compositions for expressing negative-sense viral RNA in canine cells
PA8813901A1 (es) Paramixovirus oncolíticos atenuados que codifican citoquinas de aves
MX387420B (es) Producción de virus en cultivos celulares.
BR112022000026A2 (pt) Métodos e composições compreendendo nível reduzido de proteínas de células hospedeiras
IN2014CN03077A (es)
WO2013030176A3 (en) Live attenuated influenza virus
AU2015344324A8 (en) Endoxylanase mutant, enzyme composition for biomass decomposition, and method for producing sugar solution
MX2015016765A (es) Celulas aviares para la produccion mejorada de virus.
WO2010036391A3 (en) A novel rna-based expression system
SG194703A1 (en) Multistage cellulose hydrolysis and quench with or without acid
GB2458419A (en) Vector for expressing NC protein of HIV and method for producing NC protein using the same
MX2013012925A (es) Metodos y composiciones para silenciar familias de genes mediante el uso de microarn artificiales.
EA201101288A1 (ru) Способ получения плюрипотентных клеток
EA201690634A1 (ru) Новая гетеро-трансгликозилаза и ее применение
MX2012007283A (es) Composiciones para vacunas.
NZ629093A (en) Monoterpene glycosyltransferase originating from hop and method for using same
UA114603C2 (uk) Конструкція для сайленсингу гена p0 та її застосування
MX380693B (es) Produccion de manool.

Legal Events

Date Code Title Description
FA Abandonment or withdrawal