MX2013009755A - Method for the multiple detection of influenza virus type a, type b or type c by means of oligonucleotides designed to allow allelic discrimination in a multiplex rt-pcr. - Google Patents
Method for the multiple detection of influenza virus type a, type b or type c by means of oligonucleotides designed to allow allelic discrimination in a multiplex rt-pcr.Info
- Publication number
- MX2013009755A MX2013009755A MX2013009755A MX2013009755A MX2013009755A MX 2013009755 A MX2013009755 A MX 2013009755A MX 2013009755 A MX2013009755 A MX 2013009755A MX 2013009755 A MX2013009755 A MX 2013009755A MX 2013009755 A MX2013009755 A MX 2013009755A
- Authority
- MX
- Mexico
- Prior art keywords
- type
- oligonucleotides
- region
- specific
- influenza virus
- Prior art date
Links
- 108091034117 Oligonucleotide Proteins 0.000 title abstract 4
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title abstract 4
- 241000712431 Influenza A virus Species 0.000 title abstract 2
- 238000001514 detection method Methods 0.000 title abstract 2
- 241000713196 Influenza B virus Species 0.000 title 1
- 241000713297 Influenza C virus Species 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 239000002773 nucleotide Substances 0.000 abstract 2
- 125000003729 nucleotide group Chemical group 0.000 abstract 2
- 108020000948 Antisense Oligonucleotides Proteins 0.000 abstract 1
- 101150109178 M1 gene Proteins 0.000 abstract 1
- 239000000074 antisense oligonucleotide Substances 0.000 abstract 1
- 238000012230 antisense oligonucleotides Methods 0.000 abstract 1
- 230000004069 differentiation Effects 0.000 abstract 1
- 230000001747 exhibiting effect Effects 0.000 abstract 1
- 230000002068 genetic effect Effects 0.000 abstract 1
- 206010022000 influenza Diseases 0.000 abstract 1
- 230000007246 mechanism Effects 0.000 abstract 1
- 230000004048 modification Effects 0.000 abstract 1
- 238000012986 modification Methods 0.000 abstract 1
- 230000009467 reduction Effects 0.000 abstract 1
- 230000035899 viability Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/112—Disease subtyping, staging or classification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention relates to a broad-spectrum detection system having a specificity of 99% and being based on mechanisms for the multiple allelic differentiation of influenza virus type A (sub-types H1N1, H3N2, H5N1, H7N3), type B or type C. The main components of the system are sense oligonucleotides that allow allelic discrimination, said oligonucleotides exhibiting various characteristics including: a modification in the penultimate nucleotide of region 3' in order to generate a mismatch when hybridising to the target sequence of the M1 gene; the corresponding polymorphism of the sequence to be analysed being located in the last nucleotide of region 3'; and said oligonucleotides being specific for highly conserved regions having a low incidence of genetic drift. The system also comprises antisense oligonucleotides designed such that they are specific for non-conserved regions in order to guarantee their specificity for the specific region of the target sequence to be analysed. All the aforementioned forms part of one single reaction allowing the multiple differential analysis of the different types and sub-types of influenza, and leading to a reduction in the time it takes to obtain results and an increase in the viability thereof for use in epidemiological population studies.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MX2013009755A MX2013009755A (en) | 2013-08-23 | 2013-08-23 | Method for the multiple detection of influenza virus type a, type b or type c by means of oligonucleotides designed to allow allelic discrimination in a multiplex rt-pcr. |
| PCT/MX2014/000129 WO2015026219A1 (en) | 2013-08-23 | 2014-08-14 | Method for the multiple detection of influenza virus type a, type b or type c by means of oligonucleotides designed to allow allelic discrimination in a multiplex rt-pcr |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MX2013009755A MX2013009755A (en) | 2013-08-23 | 2013-08-23 | Method for the multiple detection of influenza virus type a, type b or type c by means of oligonucleotides designed to allow allelic discrimination in a multiplex rt-pcr. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2013009755A true MX2013009755A (en) | 2015-02-23 |
Family
ID=52483929
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2013009755A MX2013009755A (en) | 2013-08-23 | 2013-08-23 | Method for the multiple detection of influenza virus type a, type b or type c by means of oligonucleotides designed to allow allelic discrimination in a multiplex rt-pcr. |
Country Status (2)
| Country | Link |
|---|---|
| MX (1) | MX2013009755A (en) |
| WO (1) | WO2015026219A1 (en) |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20070184434A1 (en) * | 2005-10-17 | 2007-08-09 | Rangarajan Sampath | Compositions for use in identification of influenza viruses |
| CN102994648B (en) * | 2012-06-21 | 2015-04-08 | 宁波海尔施基因科技有限公司 | Multi-gene detection method of respiratory viruses based on capillary electrophoresis |
-
2013
- 2013-08-23 MX MX2013009755A patent/MX2013009755A/en unknown
-
2014
- 2014-08-14 WO PCT/MX2014/000129 patent/WO2015026219A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO2015026219A1 (en) | 2015-02-26 |
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