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MX2012013890A - Bateria geneticamente modificada de la especie listeria monocytogenes. - Google Patents

Bateria geneticamente modificada de la especie listeria monocytogenes.

Info

Publication number
MX2012013890A
MX2012013890A MX2012013890A MX2012013890A MX2012013890A MX 2012013890 A MX2012013890 A MX 2012013890A MX 2012013890 A MX2012013890 A MX 2012013890A MX 2012013890 A MX2012013890 A MX 2012013890A MX 2012013890 A MX2012013890 A MX 2012013890A
Authority
MX
Mexico
Prior art keywords
listeria monocytogenes
genetically modified
modified bacterium
bacterium
species listeria
Prior art date
Application number
MX2012013890A
Other languages
English (en)
Inventor
Peter Rossmanith
Karin Fruehwirth
Martin Wagner
Sabine Fuchs
Original Assignee
Merck Patent Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merck Patent Gmbh filed Critical Merck Patent Gmbh
Publication of MX2012013890A publication Critical patent/MX2012013890A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente invención se relaciona con una bacteria genéticamente modificada de la especie Listeria monocytogenes, en donde se ha eliminado el locus genómico del factor transcripcional PrfA, caracterizado porque comprende, en el nivel genómico, una secuencia artificial que actúa como un control de amplificación interno, el uso de esta bacteria, así como un método para detectar y determinar cualitativa y/o cuantitativamente la ocurrencia de Listeria monocytogenes de tipo silvestre en una muestra que se sospecha que está contaminada con el microorganismo y un kit.
MX2012013890A 2010-06-02 2011-04-29 Bateria geneticamente modificada de la especie listeria monocytogenes. MX2012013890A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP10005731 2010-06-02
PCT/EP2011/002147 WO2011150999A1 (en) 2010-06-02 2011-04-29 A genetically modified bacterium of the species listeria monocytogenes

Publications (1)

Publication Number Publication Date
MX2012013890A true MX2012013890A (es) 2012-12-17

Family

ID=44123196

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012013890A MX2012013890A (es) 2010-06-02 2011-04-29 Bateria geneticamente modificada de la especie listeria monocytogenes.

Country Status (8)

Country Link
US (1) US8808683B2 (es)
EP (1) EP2576599B1 (es)
JP (1) JP5774095B2 (es)
CN (1) CN102906106B (es)
BR (1) BR112012030682B1 (es)
ES (1) ES2539970T3 (es)
MX (1) MX2012013890A (es)
WO (1) WO2011150999A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102676506A (zh) * 2012-05-30 2012-09-19 曹际娟 单核细胞增生李斯特氏菌核酸标准样品、其建立方法及应用
US20150307923A1 (en) * 2012-12-10 2015-10-29 Fred Hutchinson Cancer Research Center Megasphaera cerevisiae system process control (spc) primers, probes, and methods
WO2016153355A2 (en) * 2015-03-26 2016-09-29 Microbiome Limited Novel isolation and amplification process control
CN106978381A (zh) * 2017-03-23 2017-07-25 上海理工大学 prfA基因缺失单核细胞增生李斯特氏菌及制备方法
WO2020182924A1 (en) * 2019-03-14 2020-09-17 Merck Patent Gmbh A novel sampling method for long-term monitoring of microbes

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5801155A (en) 1995-04-03 1998-09-01 Epoch Pharmaceuticals, Inc. Covalently linked oligonucleotide minor grove binder conjugates
DE10012540B4 (de) * 2000-03-15 2004-09-23 Vermicon Ag Oligonukleotide und Verfahren zum spezifischen Nachweis von Mikroorganismen durch Polymerase-Kettenreaktion
US7019129B1 (en) 2000-05-09 2006-03-28 Biosearch Technologies, Inc. Dark quenchers for donor-acceptor energy transfer
WO2008017097A1 (en) 2006-08-10 2008-02-14 Merck Patent Gmbh Method for isolating cells
CN101177714A (zh) * 2007-11-15 2008-05-14 上海交通大学 添加扩增内标的单核细胞增生李斯特菌pcr检测方法
CN101368203B (zh) * 2008-09-16 2012-08-08 中国计量学院 单增李斯特菌环介导等温扩增技术快速检测用引物、试剂盒和检测方法

Also Published As

Publication number Publication date
JP5774095B2 (ja) 2015-09-02
BR112012030682A2 (pt) 2020-08-25
EP2576599B1 (en) 2015-03-18
BR112012030682B1 (pt) 2021-09-21
JP2013532962A (ja) 2013-08-22
WO2011150999A1 (en) 2011-12-08
CN102906106B (zh) 2015-11-25
US8808683B2 (en) 2014-08-19
CN102906106A (zh) 2013-01-30
US20130137100A1 (en) 2013-05-30
EP2576599A1 (en) 2013-04-10
ES2539970T3 (es) 2015-07-07

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