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MX2012002565A - Purificacion de proteina mejorada a traves de una elucion de proteina a modificada. - Google Patents

Purificacion de proteina mejorada a traves de una elucion de proteina a modificada.

Info

Publication number
MX2012002565A
MX2012002565A MX2012002565A MX2012002565A MX2012002565A MX 2012002565 A MX2012002565 A MX 2012002565A MX 2012002565 A MX2012002565 A MX 2012002565A MX 2012002565 A MX2012002565 A MX 2012002565A MX 2012002565 A MX2012002565 A MX 2012002565A
Authority
MX
Mexico
Prior art keywords
protein
elution
enhanced
modified
purification
Prior art date
Application number
MX2012002565A
Other languages
English (en)
Inventor
Arick Brown
Christopher J Dowd
Asha Nandini Radhamohan
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=43649614&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2012002565(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of MX2012002565A publication Critical patent/MX2012002565A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención proporciona métodos para purificar un polipéptido que comprende una región CH2/CH3, que comprenden ligar el polipéptido a Proteína A y eludir con un gradiente de pH, empezando a un bajo pH.
MX2012002565A 2009-09-01 2010-09-01 Purificacion de proteina mejorada a traves de una elucion de proteina a modificada. MX2012002565A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US23886709P 2009-09-01 2009-09-01
US25343809P 2009-10-20 2009-10-20
PCT/US2010/047448 WO2011028753A1 (en) 2009-09-01 2010-09-01 Enhanced protein purification through a modified protein a elution

Publications (1)

Publication Number Publication Date
MX2012002565A true MX2012002565A (es) 2012-05-29

Family

ID=43649614

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012002565A MX2012002565A (es) 2009-09-01 2010-09-01 Purificacion de proteina mejorada a traves de una elucion de proteina a modificada.

Country Status (14)

Country Link
US (3) US9428548B2 (es)
EP (2) EP3736338A1 (es)
JP (1) JP5787891B2 (es)
KR (1) KR101764449B1 (es)
CN (2) CN102686738A (es)
BR (1) BR112012004697B8 (es)
CA (1) CA2772653C (es)
ES (1) ES2787234T3 (es)
HR (1) HRP20200581T1 (es)
MX (1) MX2012002565A (es)
PL (1) PL2473617T3 (es)
RU (1) RU2571929C2 (es)
SI (1) SI2473617T1 (es)
WO (1) WO2011028753A1 (es)

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CN104411820B (zh) 2012-06-29 2017-05-03 Emd密理博公司 在蛋白纯化过程中灭活病毒的方法
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ES2735354T3 (es) * 2013-03-14 2019-12-18 Emd Millipore Corp Métodos para aumentar la pureza de proteínas utilizando la cromatografía basada en proteína A
JP6462681B2 (ja) * 2013-07-12 2019-01-30 ジェネンテック， インコーポレイテッド イオン交換クロマトグラフィーのインプットの最適化の解明
ES2970817T3 (es) * 2013-11-15 2024-05-30 Hoffmann La Roche Procedimientos para la inactivación vírica usando detergentes ecológicos
HRP20200711T1 (hr) * 2014-03-10 2020-07-24 Richter Gedeon Nyrt. Pročišćavanje imunoglobulina korištenjem koraka prethodnog čišćenja
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TWI848953B (zh) 2018-06-09 2024-07-21 德商百靈佳殷格翰國際股份有限公司 針對癌症治療之多特異性結合蛋白
US20210122783A1 (en) * 2018-06-19 2021-04-29 Bristol-Myers Squibb Company Methods of purifying proteins using chromatography
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JP2023510595A (ja) * 2020-01-17 2023-03-14 リジェネロン・ファーマシューティカルズ・インコーポレイテッド ウイルスクリアランスのための疎水性相互作用クロマトグラフィー
KR20250011797A (ko) 2023-07-13 2025-01-22 주식회사 녹십자 알부민이 융합된 단백질의 정제방법

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Also Published As

Publication number Publication date
CA2772653A1 (en) 2011-03-10
SI2473617T1 (sl) 2020-07-31
CA2772653C (en) 2019-06-25
PL2473617T3 (pl) 2020-06-29
EP2473617B1 (en) 2020-02-26
JP5787891B2 (ja) 2015-09-30
BR112012004697A2 (pt) 2015-09-08
CN102686738A (zh) 2012-09-19
CN108409829A (zh) 2018-08-17
BR112012004697B8 (pt) 2021-05-25
RU2012112536A (ru) 2013-10-10
US9428548B2 (en) 2016-08-30
HRP20200581T1 (hr) 2020-07-10
EP2473617A4 (en) 2014-04-09
ES2787234T3 (es) 2020-10-15
JP2013503877A (ja) 2013-02-04
EP2473617A1 (en) 2012-07-11
US20130041139A1 (en) 2013-02-14
EP3736338A1 (en) 2020-11-11
US20220324905A1 (en) 2022-10-13
US20220081466A1 (en) 2022-03-17
KR101764449B1 (ko) 2017-08-02
KR20120106719A (ko) 2012-09-26
WO2011028753A1 (en) 2011-03-10
BR112012004697B1 (pt) 2021-02-09
RU2571929C2 (ru) 2015-12-27

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