MX2010013435A - Larvicide and insecticide emulsifiable concentrate for spatial application, process for the preparation thereof, insecticide composition, use and control method. - Google Patents

Abstract

Described is a larvicide and insecticide emulsifiable concentrate, which contains pyrethroid insecticides combined with juvenoid larvicides IGR and excipients selected from at least a surfactant, an antievaporant, an anti-UV protector, an antioxidant, an antifoam and a suitable organic solvent. The emulsion of the concentrate, preferably in water, is spread by an environmental or superficial sprinkle in order to control flies, in general, and mosquitoes, as vectors of diseases like dengue, yellow fever and particularly malaria.

Description

A CONCENTRATE EMULSIONABLE INSECTICIDE AND LARVICIDA FOR SPACE APPLICATION. PROCEDURE FOR PREPARATION.

INSECTICIDE COMPOSITION, USE AND CONTROL METHOD FIELD OF THE INVENTION The present invention relates to compositions of emulsifiable concentrate for space application with insecticidal and larvicidal activity comprising at least one pyrethroid compound and pyriproxyfen as active compounds. The compositions of the invention have insecticidal and larvicidal activity and are compatible for space use with water as a vehicle. The present invention also relates to a method for preparing the compositions and to a method for controlling mosquito vectors of dengue, yellow fever and malaria.

BACKGROUND OF THE INVENTION The insecticides commonly called pyrethroids are currently widely used in pest control because of their excellent selectivity. In other words they have a high toxicity on insect pests although low in mammals (Naumann K. 1990, Synthetic Pyrethroid Insecticides, Chemistry and Patents, Springer Verlag, Berlin, Ruigt S. F. 1985, Pyrethroids, pp 183- 262. In G. Kerkut and L Gilbert (editors) Comprehensive Insect Physiology, Biochemistry and Pharmacology, Vol 12, Pergamon, Oxford).

Pyrethroids are excellent insecticides for the control of adult flying forms of diptera in general and of mosquitoes in particular (Pest and Vector Control, H. van Emden and M. Service, Cambridge University Press, Cambridge, UK, 2004).

The Argentine patent No. AR004978B1, which is incorporated herein by reference, describes a method of enrichment in the cis isomer of permethrin, and its separation as a crystalline solid. Also the Argentine patent application, published No. AR025747A1, which is incorporated herein by reference, describes an emulsifiable emulsifiable concentrate of cis-permethrin, a process for its preparation and its use. This product has demonstrated a good effectiveness in laboratory and field in the control of adult mosquito vectors of the dengue species Aedes aegypti in space treatments. ("Laboratory and scaled up evaluation of Cis-permethrin applied as a new Ultra Low Volume formulation against Aedes aegypti (Diptera: culicidae)" Seccacini Emilia, Masuh Hector, Licastro Susana A. de and Zerba Eduardo, Acta Trópica 97, 1-4 (2006).

The conventional formulations of permethrin are prepared with the mixture of its cis-trans isomers (Pesticide Manual, 11 th Edition, CDS Tomlin Ed. British Crop Protection Council, UK 1997) and the emulsifiable concentrates of this pyrethroid have also been shown in good spatial treatments. effectiveness on diptera in general and mosquitoes in particular (Pest and Vector Control, H. van Emden and M. Service, Cambridge University Press, Cambridge, UK, 2004).

A series of compounds have in common their mode of action, which is to interfere in the processes involved in the growth of insects, which is why they are called insect growth regulators, known by their acronym in English IGR (Insect Growth Regulators). The IGR, for its selectivity and for its very low toxicity in mammals are a group of compounds nowadays widely used in the control of insects in general (Chemical Pesticides, Mode of Action and Toxicology, J. Stenersen, CRC Press, Boca Raton , USA, 2004)) and mosquito larvae in particular, (Guidelines for Dengue Surveillance and Mosquito Control, World Health Organization, Regional Office for the Western Pacific, Second Edition, Manila, Philippines (2003)).

There are two main types of IGR, inhibitors of chitin synthesis and juvenoids, which act as analogues of juvenile hormones.

Juvenoid IGRs act by interfering with the maturation of the insect to its adult form, since they are mimics of juvenile hormones (Chemical Pesticides, Mode of Action and Toxicology, J. Stenersen, CRC Press, Boca Raton, USA, 2004). This type of compounds has been recognized as having the greatest larvicidal effect in drinking water treatments for the control of mosquitoes. { Pesticides and their application for the control of Vectors and pests of Public health importance, Sixth Edition, WHO / CDS / NTD / WHOPES / GCDPP / 2006.1, World Health Organization, 2006). One of these juvenoid compounds, 2- (1-methyl-2- (4-phenoxyphenoxy) ethoxy) pyridine, called piriproxifen or pyriproxyfen, has recently been recommended by the World Health Organization for use in larvae control. mosquitoes in waters for human consumption because of their very high larvicidal efficacy and safety of use (Pyriproxyfen in drinking water, WHO / SDE WSH / 07.01 / 10, World Health Organization, 2007).

The formulations of emulsifiable concentrate (CE) or ultra low volume (ULV) of insecticides, applied spatially as thermo fogs or cold mists are the most used forms for the control of flying adults of plague insects with immature forms larvae and adult flying forms, which live in different habitats such as mosquitoes (Chavasse DC and Yap HH, Chemical Methods for the Control of Vectors and Pests of Public Health Importance, WHO / CDT WHOPES / 97.2 pp27, Geneva, Switzerland, 1997). One of the usual criticisms that are made to this type of treatment is its insufficient effectiveness on larvae. (Masuh H., Coto H., Licastro S. and Zerba E. "Control of Aedes aegypti (L.) in Clorinda: a model for urban areas." Entomol.Vect.10 (4) 485-494 (2003)) . Also another limitation of this type of formulations for spatial treatments is that when they require the addition of diluent, for their effectiveness to be maximum they must be diluted with organic solvents, particularly gas oil and not with water. This requirement responds to the fact that when water is used as a vehicle instead of organic solvents, the effectiveness of control is reduced of insecticides on insect pests (Toxicology oí Insecticides, F. Matsumura, Plenum Press, London, 1975).

There is a continued need to prepare new compositions of the emulsifiable concentrate type, mainly applicable as ultra low volume or thermoniebla for the control of insect pests in general and diptera such as mosquitoes in particular, which are friendly to the environment. For these compositions to have a low environmental impact, water should be used as a vehicle without diminishing its adulticidal and larvicidal effectiveness by "solvent effect" or by decomposition of active substances when applied as an aqueous emulsion thermoseal.

BRIEF DESCRIPTION OF THE INVENTION It is an object of the present invention an emulsionable concentrate composition insecticide and chemically stable and compatible for space use with water as a vehicle, for control of insect pests containing as active ingredients a combination of at least one pyrethroid insecticide and the larvícida juvenoide piriproxifen and whose spatial application in aqueous emulsion presents similar or greater effectiveness to that obtained with organic diluents.

The formulations of the invention combine a pyrethroid with excellent adulticidal action and piriproxifen, a juvenoid with great effect larvicide, in compositions chemically compatible with the use of water as a vehicle, maintaining its effectiveness even in applications in the form of thermoniebla in aqueous medium. In this way spatial treatments can be carried out using water as a vehicle even in the form of a thermoniebla, controlling the adult insect populations and eliminating the reservoirs of live larvae with the same or greater effectiveness than if an organic solvent were used as a medium for the application.

The invention preferably relates to an insecticidal and larvicidal emulsifiable concentrate composition for spatial use in aqueous medium for the control of pest insects comprising one or more pyrethroid compounds selected from cis-permethrin, permethrin, praletrin, cypermethrin, phenothrin, alethane, resmethrin, cyhalothrin, cyphenothrin, cyfluthrin, deltamethrin, bifenthrin, etofenprox, isomers and mixtures thereof.

The present invention is also directed to a ready-to-use insecticidal and larvicidal composition comprising an emulsion of an emulsifiable concentrate in a carrier solvent, wherein the emulsifiable concentrate comprises at least one pyrethroid insecticide and the juvenile pyriproxyfen larvicide. Preferably the carrier solvent is selected from water and organic hydrocarbons such as deodorized kerosene, gas oil, and the like. Even more preferably the solvent is water.

Another method of control of mosquito vectors of dengue fever, yellow fever and malaria, which comprises spreading by spraying over the controllable area a composition comprising an emulsion in water of the concentrate of the invention so as to provide a concentration of between 1 and 10 g of permethrin containing not less than 80% cis isomer, and between 0.2 and 3 g of pyriproxyfen per hectare of surface, still more preferably between 1 and 15 g of permethrin and between 0.2 and 3 g of pyriproxyfen per hectare of surface.

DETAILED DESCRIPTION OF THE INVENTION Surprisingly, it has been found that certain combinations of pyrethroid insecticides such as cis-permethrin or permethrin and the insect growth regulator piriproxifen can be formulated as stable products, chemically and biologically compatible with the use of water as a vehicle without losing effectiveness against the use alternative organic solvents and suitable for spatial applications, even as a cold in aqueous medium, give rise to a great adulticide and larvicide effectiveness on insect populations in general and diptera as mosquitoes in particular.

Even more preferably the invention relates to an insecticidal and larvicidal emulsifiable concentrate composition for spatial use in aqueous medium for the control of pest insects comprising as an active ingredient a pyrethroid selected from: cis-permethrin, permethrin, praletrin, cypermethrin, cyhalothrin, deltamethrin, isomers and mixtures thereof.

The pyrethroid content in the composition is between 1 and 50% (w / v), preferably between 5 and 30% (w / v).

The content of pyriproxyfen in the composition is between 0.5 and 20% (w / v), preferably between 2 and 10% (w / v).

The emulsifiable concentrate for spatial use in aqueous medium further comprises at least one excipient of the surfactant, anti-evaporation, anti-UV, antioxidant or antifoam type dissolved in a suitable solvent.

Among the surfactants that can be used are, for example, alkylarylsulfonate salts, ethoxylated polyaryl phenols, ethoxylated castor oil, nonylphenols ethoxylates, their mixtures and the like. Preferably, straight or branched calcium alkyl (C 10 -C 13) -benzene sulphonates can be used as alkylarylsulfonate salts. Preferably as ethoxylated polyaryl phenols, ethoxylated tristyrylphenol can be used. Preferably as ethoxylated castor oil ethoxylated castor oils with 36 to 40 moles of ethoxy can be used. Preferably as ethoxylated nonylphenols, ethoxylated nonylphenols with 9 to 14 moles of ethoxy can be used.

Preferably, the surfactants are present in the concentrate of the invention, in concentrations comprised between 0.5 and 16% (w / v).

Among the antievaporants that can be used are, for example, those of the vegetable oil type or esters of their fatty acids or mixtures thereof or polyethylethylene glycols and the like. Preferably as vegetable oils corn or soya oil or their mixtures can be used. Preferably, fatty acid esters of maize or soybean oil or mixtures thereof can be used as vegetable fatty acid esters. Polyethylene glycol 300 to 600 and 1000 are preferably used as polyethylene glycol.

Preferably the anti-evaporators are present in the concentrate of the invention in concentrations ranging from 2 to 50% (w / v).

Among the UV protectants that can be used in the concentrate of the invention are, for example, benzophenone, its derivatives or mixtures thereof and the like.

Preferably the UV protector used is the benzophenone.

Preferably the UV protectants are present in the concentrate of the invention in concentrations comprised between 0.1 and 5% (w / v).

Among the antioxidants that can be used in the concentrate of the invention are, for example, the alkyl gallates, butyl hydroxy toluene, butyl hydroxy anisole, nordihydroguaiaretic acid, tocopherols, thiodipropionic acid or their mixtures and the like.

Preferably, the antioxidants are present in the concentrate of the invention in concentrations comprised between 0.1 and 4% (w / v).

The defoamers that can be used in the concentrate of the invention are, for example, of the type of silicones, fatty alcohols or their mixtures, such as synthetic silicones, octanol, nonanol and their mixtures and the like.

Preferably, the defoamers are present in the concentrate of the invention in concentrations comprised between 0.01 and 1% (w / v).

The snts which can be used in the concentrate of the invention are, for example, of the organic type such as aromatic hydrocarbons such as toluene, xylene etc., ketones such as cyclohexanone, N-methyl-2-pyrrolidone, 2-pyrrolidone, etc. or its mixtures and the like.

The snts in the concentrate of the invention are used to complete the 100% formulation.

Another object of the present invention is a process for preparing the emulsifiable concentrate described above. The process for preparing an emulsifiable concentrate of pyrethroid insecticide and juvenoid larvicide, of the invention, comprises adding a pyrethroid insecticide and a juvenoid larvicide, and at least one excipient selected from a surfactant, an anti-evaporator, an anti-UV protector, a antioxidant and an antifoam in a suitable snt, and stir until dissd.

The procedure can be carried out by adding to a determined volume of adequate snt, a ratio of between 1.25 and 55% of a pyrethroid insecticide and between 1 and 25% of a juvenoid larvicide. Next, at least one excipient selected from a surfactant, an anti-evaporator, an anti-UV protector, an antioxidant and an antifoam is incorporated.

Among the surfactants which can be used in the process of the invention, mention may be made, by way of example, of the type of alkylarylsulfonate salts, ethoxylated polyaryl phenols, ethoxylated castor oil, ethoxylated nonylphenols or their mixtures and the like. Preferably, the surfactants are added to the initial mixture in concentrations between 0.5 and 16% (w / v).

Among the antievaporants which can be used in the process of the invention, mention may be made, by way of example, of the type of vegetable oils, esters of their fatty acids or mixtures thereof or polyethylethylene glycols and the like. Preferably the anti-evaporators are added to the composition of the invention in concentrations between 2 and 50% (w / v).

Among the UV protectants which can be used in the process of the invention, benzophenone, its derivatives or mixtures thereof and the like are mentioned by way of example. Preferably the UV protectors they are added to the composition of the invention in concentrations comprised between 0.1 and 5% (w / v).

Among the antioxidants which can be used in the process of the invention, mention may be made, for example, of the alkyl gallates, butyl hydroxy toluene, butyl hydroxy anisole, nordihydroguaiaretic acid, tocopherols, thiodipropionic acid or mixtures thereof and the like. Preferably the antioxidants are added to the composition of the invention in concentrations comprised between 0.1 and 4% (w / v).

The defoamers used in the process of the invention can be, for example, the type of silicones, fatty alcohols or the like and their mixtures. Preferably, antifoams are added in concentrations between 0.01 and 1.0% (w / v).

The snts that can be used in the process of the invention are of the organic type such as aromatic hydrocarbons such as toluene, xylene etc., ketones such as cyclohexanone, N-methyl-2-pyrrolidone, 2-pyrrolidone, etc., and the like , or their mixtures, in sufficient quantity to adjust the final content of the active compounds, as previously mentioned.

For its application, the concentrate of the invention is preferably emulsified in a liquid carrier in a ratio by volume of concentrate: carrier from about 1: 1000 to about 1: 2. Preferably the ratio in concentrated volume: carrier is from about 1: 500 to about 1: 3, more preferably from about 1: 300 to about 1: 4, even more preferably from about 1: 100 to about 1: 5. The liquid carrier is selected from water and organic solvents such as deodorized kerosene, diesel oil, biodiesel, etc. Preferably the liquid carrier is water.

The composition obtained from the concentrate of the invention is especially effective for combating pests in general, and for the spatial control of diptera such as flies and mosquitoes in particular.

The emulsifiable formulations of the invention are particularly suitable to be used as an emulsion in water for ultra-low volume (cold mist) or heat foggy (sparks) space treatments without the solvent effect or thermal decomposition losing effectiveness with dilutions with organic solvents in the control of dipterous insects in general and mosquito vectors of dengue (Aedes aegypti) in particular.

Suitable doses for environmental treatments are considered from 1 to 25 g of total active compounds per hectare.

The composition obtained from the concentrate of the present invention is particularly apt to be applied by means of spray devices such as thermonebulizers, cold mist generators, fixed high pressure sprayers with motor pumps, backpack sprayers with a pump, backpack sprayers. with piston pump or with diaphragm pump, open container sprayers with stirrup pump, manual intermittent or compression sprayers, trigger sprayers, and other devices generally known in the art.

The formulated emulsifiable concentrate of pyrethroid insecticide and juvenoid pyriproxygen larvicide based on easily accessible components, through its possibility of being applied as a cold mist or thermoniebla in aqueous medium with the same or greater effectiveness than in organic solvent on larvae and adult insects, improves and simplifies control strategies for insect populations in general and diptera, such as mosquitoes in particular, with a lower environmental impact and greater safety of use.

Having described the invention in a general manner, it will be better understood in relation to the following specific examples, which are included for the sole purpose of illustrating the invention, and should not be construed as limiting it.

EXAMPLES EXAMPLE 1 0% (w / v) permethrin (98% isomer c / 's), 2% pyriproxyfen, 10% (w / v) of a mixture of calcium dodecyl benzenesulfonate and tristyrylphenol ethoxylate, 10% (p. / v) of soybean oil and 0.1% (w / v) of synthetic silicone to an amount of xylene in sufficient quantity to reach 100%. The components were stirred vigorously until dissolved.

The concentration of permethrin (c / s 98%) was verified in the formula prepared by HPLC, on RP-18 column, 25 cm in length and 4.6 mm in diameter, with acetonitrile: water in a 75:25 ratio at a flow constant 1.5 ml / min using ultraviolet detector at 234 nm. To confirm the concentration of pyriproxyfen in the prepared formulation, a 25 cm column, MICROSORV PHENYL and acetonitrile / water was used as an elution solvent in a 65:35 ratio; flow 1 ml / min. The detection was made by ultraviolet detector at 234 nm.

The larvicidal and adulticidal activity on Aedes aegypti of the formulation described in a laboratory scale test was evaluated in cubic glass chamber of 0.35 m3. For this purpose, a spray was made by manual sprayer Environmentally acceptable solution with an aqueous dilution (0.85 ml of formulated in 50 ml of water) of the formulation through a hole with screw cap on the front face of the chamber to reach a final concentration of 5 mg / m3 of cis-permethrin and 1 mg / m3 of piriproxifen in aqueous emulsion inside the chamber. Inside were placed 4 disposable containers in the lower corners containing 250 ml of water with 20 larvae III of Aedes aegypti. Likewise, 3 metal mesh cages were placed with 10 adult mosquitoes of the same species each hanging from the upper part of the chamber.

The aqueous dilution of the described formulation was sprayed and the chamber was sealed for 1 h. The camera was opened, ventilated and removed the cages with adults and the pots with larvae. The adult mortality in the cages was evaluated at 2 hours of cage withdrawal and the non-emergency of adults after the emergence of all the adults in the control (13-15 days) as a parameter indicating the larvicidal effect by interruption of larval development.

The results obtained were 100% of adult mortality and 0% of hatching of adults, which indicates the total mortality of the exposed larvae. A control test was conducted with a formulation without active compounds, observing that the emergence of adults was 100%.

EXAMPLE 2 10% (w / v) of permethrin, 2% of pyriproxyfen, 10% (w / v) of a mixture of calcium dodecylbenzenesulfonate and ethoxylated tristyrylphenol, 10% (w / v) of soybean oil and 0.1% were added. (p / v) synthetic silicone to an amount of xylene in sufficient quantity for 100%. The components were stirred vigorously until dissolved. The content in assets was analyzed as described in example 1.

With this composition, pre-field tests were made by sprinkling with an aqueous dilution of the described formulation of permethrin and pyriproxyfen in a 420 m3 shed making the application from one end of the house. A dilution of 17.5 ml of formulated in 5 liters of water was used. Additionally, two trials were carried out using dilutions of the emulsifiable formulation of permethrin and pyriproxyfen prepared as described, in two organic solvents: diesel and biodiesel for comparative purposes. In each experiment 330 ml of the dilution (sprinkled for 1 minute) were applied and distributed in the house.

Adult mosquitoes (Aedes aegypti) were confined in disposable plastic cages covered with gauze and larvae III in disposable plastic containers containing 250 ml of water. The containers with mosquitoes were placed in racks at a height of 1.5 m from the floor and 3, 6 and 9 m from the spray machine.

A portable Motan machine was used to spray with drop size ULV in the above described shed.

Adult mortality was counted after 24 hours of treatment.

The growth and mortality of larvae was followed until the total hatching (100%) of adults in the controls and at that time the non-emergence of adults in the treated vessels was determined as a parameter indicating the larvicidal effect due to interruption of larval development.

The results obtained from the effect on adult mosquitoes and larvae are summarized in Table 1.

TABLE 1 Mortality of adult mosquitoes and larvae at different distances from the formulated application * Measured by not hatching adults. The adult hatch in the controls was 100%.

The results of the pre-field experience summarized in the Table 1 show that the application of the emulsifiable concentrate formula of permethrin + pyriproxyfen of the present invention, when applied spatially as an aqueous emulsion, it has an adulticidal effect on mosquitoes of the Aedes aegypti species similar to that obtained by the same formulated vehicled in organic solvents. On the other hand, surprisingly, the application of a formulation of the invention in emulsion aqueous showed a larvicidal effect superior to that obtained from the same formulation vehiculated in organic solvents.

EXAMPLE 3 15% (w / v) permethrin, 3% pyriproxyfen, 12% (w / v) of a mixture of calcium dodecyl benzenesulfonate and nonyl phenol ethoxylated, 10% sunflower oil and 0.1% (w / v) are added ) of synthetic silicone to an amount of xylene in sufficient quantity for 100%. The components are stirred vigorously until dissolved.

The concentration of permethrin and pyriproxyfen in the emulsifiable formulation was confirmed by HPLC according to that described in example 1. In the case of permethrin, as indicated for c / 's-permethrin.

The adulticidal and larvicidal effect of the described formulation was measured under laboratory conditions in chamber of 0.35 m3 with final active concentrations of 7.5 mg / m3 of permethrin and 1.5 mg / m3 of piriproxifen inside the chamber and with the methodology described in the example 1.

The results obtained were 100% of adult mortality and 0% of hatching of adults (in the control test the emergence of adults was 100%).

EXAMPLE 4 A comparative field trial was conducted in the towns of Puerto Libertad and Wanda, located in the province of Misiones, Argentina (25 ° 57'51.85"S and 54 ° 35'22.90" W).

To perform the test, an emulsifiable concentrate formulation of 10% permethrin and 2% pyriproxyfen prepared according to Example 1 was used. Dilutions in water and in diesel oil were tested separately in various treatment schemes. Four areas were determined completely separated from each other, each with approximately 200 homes. In each of these zones, the following treatments were carried out: Zone 1: Application of hot mist using solvent water.

Zone 2: Application of hot fog using diesel solvent.

Zone 3: Application of cold fog using solvent as water.

Zone 4: Control Zone, without treatment.

The evaluation of insecticidal effectiveness was carried out on larvae and adults of Aedes aegypti (Diptera: Culicidae).

To carry out the evaluation of the adulticidal effect, 10 adults of Aedes aegypti of 36-48 hours of life were transferred to bioassay cages in the laboratory, which were transported to the field one hour before treatment and were placed between three and five meters of the application line. 10 cages per zone were placed.

To evaluate the larvicidal activity, 10 plastic containers, white and opaque, of 250 ml with drinking water per zone were placed. They were placed between three and five meters from the application line and inside the houses. 15 Aedes aegypti larvae III / IV were placed per container.

The application of the formulations at ultra low volume (ULV) was made with cold fog generating machine 2P ™ ULV, Maxi-Pro ™ (Curtis Dyna-Fog Ltd. Westfield, Indiana, USA) and heavy machine generating hot fog SWingfog SN 101 (Motan Swingtec GMBH, Isny, Germany) each mounted on the back of a truck.

Three insecticidal compositions were prepared for application in each zone, in addition to the control composition (without active compounds).

- Composition for cold fog: 6.6 liters of insecticidal formula, 1.25 liters of polyethylene glycol 1000 and 17.15 liters of water.

- Composition for hot fog: 5.2 liters of insecticide formula, 1 liter of polyethylene glycol 1000 and 14 liters of water.

- Composition for hot fog: 5.2 liters of insecticide formula, 1 liter of polyethylene glycol 1000 and 14 liters of diesel oil.

Using the cold fog machine, 379 cm3 of insecticide mixture was released every 36 seconds at a vehicle speed of 10 km per hour. The hot fog machine released 390 cm3 of insecticide mixture every 36 seconds at a vehicle speed of 10 km per hour To evaluate the effectiveness of each type of application, a single application dose of 10 grams of permethrin / hectare and 2 grams of pyriproxyfen / hectare was used.

After approximately one hour of treatment, the adult cages and 250 ml containers were taken to the laboratory to determine mortality, which was carried out after 24 hours of treatment. finished treatments, and emergency inhibition of adults on larvae exposed to spray.

The following results were obtained: TABLE 2 Comparative values of mortality of adult mosquitoes and larvae with different treatments * Measured by not hatching adults. Hatching of adults in controls 100% The results of the field experience summarized in Table 2 demonstrate that the application of the emulsifiable concentrated formula of permethrin + pyriproxyfen of the present invention, when applied spatially as an aqueous emulsion, has an adulticidal and larvicidal effect on mosquitoes of the Aedes aegypti species superior to the one that demonstrates the same formulation vehicled in an organic solvent.

Claims (29)

NOVELTY OF THE INVENTION CLAIMS

1. - An emulsifiable insecticidal and larvicidal concentrate for insect control suitable for spatial treatments diluted with water characterized in that it comprises a combination of one or more insecticidal pyrethroid compounds and the mimic of the juvenile hormone of insects piriproxifen and suitable excipients selected from at least one surfactant, an anti-evaporator, an anti-UV protector, an antioxidant, an antifoam and a suitable organic solvent.

2. - The insecticidal and larvicidal emulsifiable concentrate according to claim 1, further characterized in that said pyrethroid insecticides are selected from: c / 's-permethrin, permethrin, praletrin, cypermethrin, phenothrin, allethrin, resmethrin, cyhalothrin, cyphenothrin, cyfluthrin, deltamethrin , bifenthrin, etofenprox, its isomers and combinations thereof.

3. - The insecticidal and larvicidal emulsifiable concentrate according to claim 1, further characterized in that the pyrethroid insecticide is c / 's-permethrin, permethrin, praletrin, cypermethrin, cyhalothrin, deltamethrin, its isomers or a combination thereof.

4. - The insecticidal and larvicidal emulsifiable concentrate according to claim 1, further characterized in that the pyrethroid insecticide is permethrin or its cis isomer.

5. - The insecticidal and larvicidal emulsifiable concentrate according to any of the preceding claims, further characterized in that the pyrethroid insecticide content is comprised between 1 and 50% w / v.

6. - The insecticidal and larvicidal emulsifiable concentrate according to claim 5, further characterized in that the content of pyrethroid insecticide is between 5 and 30% w / v.

7. - The insecticidal and larvicidal emulsifiable concentrate according to claim 6, further characterized in that the pyrethroid insecticide content is 10% w / v.

8. - The insecticidal and larvicidal emulsifiable concentrate according to claim 1, further characterized in that the juvenoid pyriproxyfen larvicide is present in a proportion comprised between 0.5 and 20% w / v.

9. - The insecticidal and larvicidal emulsifiable concentrate according to claim 8, further characterized in that the juvenoid pyriproxyfen larvicide is present in a proportion comprised between 2 and 10% w / v.

10. - The insecticidal and larvicidal emulsifiable concentrate according to claim 1, further characterized in that the pyrethroid is c / 's-permethrin.

11. - The insecticidal and larvicidal emulsifiable concentrate according to claim 1, further characterized in that the pyrethroid is permethrin.

12. - The insecticidal and larvicidal emulsifiable concentrate according to claim 1, further characterized in that it contains a surfactant selected from alkylarylsulfonate salts, ethoxylated polyaryl phenols, ethoxylated castor oil, ethoxylated nonylphenols and combinations thereof.

13. - The insecticidal and larvicidal emulsifiable concentrate according to claim 12, further characterized in that the surfactant is selected from linear or branched calcium (C10-C13) alkylbenzenesulfonate, ethoxylated tristyrylphenol, ethoxylated castor oil with 36 to 40 moles of ethoxy , nonylphenol ethoxylated with 9 to 14 moles of ethoxy and combinations thereof.

14. - The insecticidal and larvicidal emulsifiable concentrate according to claim 1, further characterized in that it contains between 2 and 50.0% (w / v) of an antievaporant selected from vegetable oils or esters of their fatty acids or mixtures thereof or polyethylethylene glycols and combinations thereof.

15. - The insecticidal and larvicidal emulsifiable concentrate according to claim 1, further characterized in that it contains between 0.1 and 5% (w / v) of an anti-UV protector selected from benzophenone, its derivatives and combinations thereof.

16. - The insecticidal and larvicidal emulsifiable concentrate according to claim 1, further characterized in that it contains between 0.1 and 4% (w / v) of an antioxidant selected from alkyl gallates, butyl hydroxy toluene, butyl hydroxy anisole, nordihydroguaiaretic acid, tocopherols, acid thiodipropionic and combinations thereof.

17. - The insecticidal and larvicidal emulsifiable concentrate according to claim 1, further characterized in that it contains between 0.01 and 1% (w / v) of an antifoam selected from silicones, fatty alcohols and combinations thereof.

18. - The insecticidal and larvicidal emulsifiable concentrate according to claim 1, further characterized in that the solvent is selected from aromatic hydrocarbons, ketones and combinations thereof.

19. - The insecticidal and larvicidal emulsifiable concentrate according to claim 18, further characterized in that the solvent is selected from toluene, xylene, cyclohexanone, N-methyl-2-pyrrolidone and combinations thereof.

20. - A process for the preparation of an insecticidal and larvicidal emulsifiable concentrate of claim 1, characterized by add permethrin, pyriproxyfen and at least one excipient selected from a surfactant, an anti-evaporator, an anti-UV protector, an antioxidant and an antifoam to a sufficient amount of suitable organic solvent and dissolve by stirring.

21. - A ready-to-use insecticidal and larvicidal composition, consisting of an emulsion of the claimed emulsifiable concentrate of any of claims 1 to 19, in a liquid carrier wherein the ratio of concentrate: carrier is from about 1: 1000 to about 1: 2.

22. - The insecticidal and larvicidal composition ready for use, according to claim 21, further characterized in that the ratio of concentrate: carrier is from about 1: 300 to about 1: 4.

23. - The insecticidal and larvicidal composition ready for use, according to claim 21, further characterized in that the ratio of concentrate: carrier is from about 1: 100 to about 1: 5.

24. - The insecticidal and larvicidal composition ready for use, according to any of claims 21-23, further characterized in that the carrier is selected from water and a solvent.

25. - The insecticidal and larvicidal composition ready for use, according to any of claims 21-23, further characterized in that the carrier is water.

26. - The use of an insecticidal and larvicidal composition of claim 21 for the control of mosquitoes.

27. - The use of an insecticidal and larvicidal composition as claimed in claim 26 for the control of the mosquito / Aedes aegypti vector of dengue and yellow fever.

28. - A method of control of mosquito vectors of dengue, yellow fever and malaria, characterized in that a composition of any of claims 21 to 25 is sprayed onto the controllable area in order to provide a concentration of between 1 and 10 g of permethrin containing not less than 80% cis isomer, and between 0.2 and 3 g of pyriproxyfen per hectare of surface.

29. - A method of control of mosquito vectors of dengue, yellow fever and malaria, characterized in that a composition of any of claims 21 to 25 is sprayed onto the controllable area in order to provide a concentration of between 1 and 15 g of permethrin and between 0.2 and 3 g of pyriproxyfen per hectare of surface.