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MX2010005111A - Metodo para la deteccion y/o analisis de levaduras y moho en liquidos filtrables. - Google Patents

Metodo para la deteccion y/o analisis de levaduras y moho en liquidos filtrables.

Info

Publication number
MX2010005111A
MX2010005111A MX2010005111A MX2010005111A MX2010005111A MX 2010005111 A MX2010005111 A MX 2010005111A MX 2010005111 A MX2010005111 A MX 2010005111A MX 2010005111 A MX2010005111 A MX 2010005111A MX 2010005111 A MX2010005111 A MX 2010005111A
Authority
MX
Mexico
Prior art keywords
sample
yeast
food
analysis
mold
Prior art date
Application number
MX2010005111A
Other languages
English (en)
Inventor
Frank R Burns
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of MX2010005111A publication Critical patent/MX2010005111A/es

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

Esta invención es un proceso mejorado para preparar una muestra de alimento o de bebida que contiene células de levaduras y moho para la prueba analítica. La muestra de alimento es preparada en la forma de un líquido filtrable, y luego filtrada utilizando un filtro de microfibras de vidrio. El filtro que contiene el retenido de células de moho es luego colocado en un recipiente de desintegración y batido con esferas hasta que el filtro de microfibras de vidrio es completamente desintegrado en fibras de vidrio en suspensión. Puede ser luego probada una alícuota directamente utilizando el análisis de la curva de fusión del producto de amplificación por PCR, derivado de los ácidos nucleicos de la muestra, para detectar la presencia de las células de moho a partir de la muestra.
MX2010005111A 2007-11-16 2008-11-12 Metodo para la deteccion y/o analisis de levaduras y moho en liquidos filtrables. MX2010005111A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US334507P 2007-11-16 2007-11-16
PCT/US2008/083204 WO2009064766A1 (en) 2007-11-16 2008-11-12 Method for detection and/or analysis of yeast and mold in filterable liquids

Publications (1)

Publication Number Publication Date
MX2010005111A true MX2010005111A (es) 2010-05-27

Family

ID=40227626

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2010005111A MX2010005111A (es) 2007-11-16 2008-11-12 Metodo para la deteccion y/o analisis de levaduras y moho en liquidos filtrables.

Country Status (8)

Country Link
US (1) US8835109B2 (es)
EP (1) EP2222847A1 (es)
JP (1) JP5659018B2 (es)
AU (1) AU2008321063B2 (es)
BR (1) BRPI0817166A2 (es)
CA (1) CA2703735A1 (es)
MX (1) MX2010005111A (es)
WO (1) WO2009064766A1 (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017116694A1 (en) * 2015-12-29 2017-07-06 3M Innovative Properties Company Method and system for detecting microorganisms in large-volume samples
EP3441477A1 (en) * 2017-08-08 2019-02-13 Microgenetics Limited Method for detecting a microorganism in a sample
KR102237035B1 (ko) * 2019-10-14 2021-04-06 성균관대학교산학협력단 여과액을 이용한 곰팡이 검출용 전기화학센서
JP7428326B2 (ja) * 2021-11-02 2024-02-06 愛知県 検体中の核酸を回収する方法

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5145786A (en) 1990-09-21 1992-09-08 The United States Of America As Represented By The Secretary Of Agriculture Preenriched broth medium for the simultaneous sampling of foods for salmonella and listeria
US5846717A (en) 1996-01-24 1998-12-08 Third Wave Technologies, Inc. Detection of nucleic acid sequences by invader-directed cleavage
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US6312930B1 (en) 1996-09-16 2001-11-06 E. I. Du Pont De Nemours And Company Method for detecting bacteria using PCR
JP4456182B2 (ja) 1996-09-16 2010-04-28 ザ カバメント オブ ザ ユーナイテッド ステイツ オブ アメリカ,リプリゼンテッド バイ ザ セクレタリー,デパートメント オブ ヘルス アンド ヒューマン サービシーズ,センターズ フォー ディジ Candida spp.の検出のための方法および組成物
WO1998020148A1 (en) 1996-11-04 1998-05-14 The Regents Of The University Of California Method for detection of pathogens in food
EP1253203A1 (en) 2001-04-25 2002-10-30 Becton Dickinson and Company Rapid resuscitation, growth, capture and detection of microorganisms
AU2002354838B2 (en) 2001-07-19 2007-11-29 Infectio Diagnostic (I.D.I.) Inc. Universal method and composition for the rapid lysis of cells for the release of nucleic acids and their detection
US7291465B2 (en) * 2004-02-20 2007-11-06 Karaolis David K R Method for direct detection of fungal pathogens
US7776530B2 (en) * 2004-06-29 2010-08-17 Wallac Oy Integrated nucleic acid analysis

Also Published As

Publication number Publication date
WO2009064766A1 (en) 2009-05-22
AU2008321063B2 (en) 2015-04-23
CA2703735A1 (en) 2009-05-22
JP5659018B2 (ja) 2015-01-28
EP2222847A1 (en) 2010-09-01
JP2011502536A (ja) 2011-01-27
US8835109B2 (en) 2014-09-16
US20110104672A1 (en) 2011-05-05
AU2008321063A1 (en) 2009-05-22
BRPI0817166A2 (pt) 2014-10-14

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