[go: up one dir, main page]

MX2010003644A - Ensamble de acidos nucleicos grandes. - Google Patents

Ensamble de acidos nucleicos grandes.

Info

Publication number
MX2010003644A
MX2010003644A MX2010003644A MX2010003644A MX2010003644A MX 2010003644 A MX2010003644 A MX 2010003644A MX 2010003644 A MX2010003644 A MX 2010003644A MX 2010003644 A MX2010003644 A MX 2010003644A MX 2010003644 A MX2010003644 A MX 2010003644A
Authority
MX
Mexico
Prior art keywords
assembly
nucleic acids
large nucleic
cassettes
recombination
Prior art date
Application number
MX2010003644A
Other languages
English (en)
Inventor
J Craig Venter
Daniel G Gibson
Gwynedd A Benders
Lei Young
John I Glass
Clyde A Hutchison Iii
Hamilton O Smith
Original Assignee
Synthetic Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Synthetic Genomics Inc filed Critical Synthetic Genomics Inc
Publication of MX2010003644A publication Critical patent/MX2010003644A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Se describe un método para ensamblar cualquier molécula de ácido nucleico deseada al combinar casetes in vitro para formar ensambles que además se combinan in vivo, o al ensamblar números grandes de fragmentos de DNA por recombinación en un cultivo de levadura para obtener moléculas de DNA deseadas de tamaño sustancial.
MX2010003644A 2007-10-08 2008-10-07 Ensamble de acidos nucleicos grandes. MX2010003644A (es)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
US97838807P 2007-10-08 2007-10-08
US98354907P 2007-10-29 2007-10-29
US6221408P 2008-01-23 2008-01-23
US2339208P 2008-01-24 2008-01-24
US9627008P 2008-09-11 2008-09-11
PCT/US2008/079109 WO2009048885A2 (en) 2007-10-08 2008-10-07 Assembly of large nucleic acids

Publications (1)

Publication Number Publication Date
MX2010003644A true MX2010003644A (es) 2010-06-23

Family

ID=40549815

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2010003644A MX2010003644A (es) 2007-10-08 2008-10-07 Ensamble de acidos nucleicos grandes.

Country Status (11)

Country Link
US (1) US9593329B2 (es)
EP (1) EP2207899B1 (es)
JP (1) JP5618413B2 (es)
CN (1) CN101932725A (es)
AU (1) AU2008311000B2 (es)
CA (1) CA2701923C (es)
DK (1) DK2207899T3 (es)
IL (1) IL204892B (es)
MX (1) MX2010003644A (es)
MY (1) MY161418A (es)
WO (1) WO2009048885A2 (es)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9267132B2 (en) 2007-10-08 2016-02-23 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
WO2010045326A1 (en) 2008-10-14 2010-04-22 Barnes Wayne M In vitro recombination methods using 5' exonuclease
MX2011005195A (es) * 2008-11-19 2011-09-01 Amyris Inc Composiciones y metodos para el ensamblaje de polinucleotidos.
EP2403944B1 (en) 2009-03-06 2017-06-28 Synthetic Genomics, Inc. Methods for cloning and manipulating genomes
KR20120039047A (ko) 2009-07-31 2012-04-24 노파르티스 아게 역유전학 시스템
US20110269119A1 (en) 2009-10-30 2011-11-03 Synthetic Genomics, Inc. Encoding text into nucleic acid sequences
CN102939380A (zh) * 2010-03-05 2013-02-20 合成基因组股份有限公司 用于克隆和操作基因组的方法
WO2012051327A2 (en) 2010-10-12 2012-04-19 Cornell University Method of dual-adapter recombination for efficient concatenation of multiple dna fragments in shuffled or specified arrangements
CN103945931B (zh) 2011-09-26 2017-03-22 基因技术股份公司 高效的小体积核酸合成
WO2014153188A2 (en) 2013-03-14 2014-09-25 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
US20140303036A1 (en) * 2011-11-23 2014-10-09 Dsm Ip Assets B.V. Nucleic Acid Assembly System
WO2013191950A2 (en) 2012-06-22 2013-12-27 Monsanto Technology Llc Unique modular vector design
US9206435B2 (en) * 2012-08-31 2015-12-08 Synthetic Genomics, Inc. Crowding agent-induced nucleic acid transfer into a recipient host cell
WO2014093535A1 (en) * 2012-12-13 2014-06-19 Synthetic Genomics, Inc. Peg-mediated assembly of nucleic acid molecules
WO2014115104A1 (en) * 2013-01-23 2014-07-31 Novartis Ag Influenza virus reassortment
US20140274806A1 (en) 2013-03-15 2014-09-18 Synthetic Genomics Vaccines Influenza virus reassortment
WO2016007604A1 (en) 2014-07-09 2016-01-14 Gen9, Inc. Compositions and methods for site-directed dna nicking and cleaving
CA3147259A1 (en) 2014-12-09 2016-06-16 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
CN106282224B (zh) * 2015-06-26 2020-05-01 深圳华大生命科学研究院 在靶细胞中构建人工染色体的方法以及真核细胞
EP3359669B1 (en) 2015-10-06 2020-05-13 Pierce Biotechnology, Inc. Devices and methods for producing proteins
CA3018199A1 (en) 2016-03-23 2017-09-28 Synthetic Genomics, Inc. Generation of synthetic genomes
US20210284991A1 (en) * 2016-11-21 2021-09-16 Novozymes A/S Yeast Cell Extract Assisted Construction of DNA Molecules
US10726110B2 (en) * 2017-03-01 2020-07-28 Seven Bridges Genomics, Inc. Watermarking for data security in bioinformatic sequence analysis
EP3814494B1 (en) 2018-06-29 2023-11-01 Thermo Fisher Scientific GENEART GmbH High throughput assembly of nucleic acid molecules
CA3106410A1 (en) 2018-07-23 2020-01-30 Dna Script Massively parallel enzymatic synthesis of nucleic acid strands
GB201905303D0 (en) 2019-04-15 2019-05-29 Thermo Fisher Scient Geneart Gmbh Multiplex assembly of nucleic acid molecules
WO2020224987A1 (en) * 2019-05-06 2020-11-12 Dsm Ip Assets B.V. Multipartite crispr donor
WO2021178809A1 (en) 2020-03-06 2021-09-10 Life Technologies Corporation High sequence fidelity nucleic acid synthesis and assembly
CN113380322B (zh) * 2021-06-25 2023-10-24 倍生生物科技(深圳)有限公司 人工核酸序列水印编码系统、水印字符串及编码和解码方法
EP4638776A1 (en) 2022-12-19 2025-10-29 Thermo Fisher Scientific GENEART GmbH Retrieval of sequence-verified nucleic acid molecules

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6348353B1 (en) * 1995-06-07 2002-02-19 Case Western Reserve University Artificial mammalian chromosome
US5976846A (en) * 1996-01-13 1999-11-02 Passmore; Steven E. Method for multifragment in vivo cloning and mutation mapping
AU6487196A (en) * 1996-07-09 1998-02-02 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Transformation-associated recombination cloning
EP1538206B1 (en) * 1997-09-16 2010-03-24 Centocor, Inc. Method for the complete chemical synthesis and assembly of genes and genomes
AU5217599A (en) * 1998-07-27 2000-02-21 Genotypes, Inc. Automatic eukaryotic artificial chromosome vector
JP2003501082A (ja) * 1999-06-07 2003-01-14 セル ジェネシス インコーポレイテッド ハイブリッド酵母−細菌クローニング系およびその使用
US7822969B2 (en) * 2001-04-16 2010-10-26 Digimarc Corporation Watermark systems and methods
AU2002332027A1 (en) * 2001-10-04 2003-04-14 The Government Of The United States Of America, As Tandem repeat markers
WO2004113534A1 (en) * 2003-05-22 2004-12-29 University Of California Method for producing a synthetic gene or other dna sequence
WO2007021944A2 (en) 2005-08-11 2007-02-22 The J. Craig Venter Institute In vitro recombination method
MY144014A (en) 2005-08-11 2011-07-29 Synthetic Genomics Inc Method for in vitro recombination
JP2009511051A (ja) 2005-10-12 2009-03-19 ザ ジェイ． クレイグ ベンター インスティテュート 最小限の細菌ゲノム
MX2010003157A (es) 2007-10-05 2010-05-21 Sapphire Energy Inc Sistema para capturar y modificar piezas grandes de adn genomico y construir organismos con cloroplastos sinteticos.

Also Published As

Publication number Publication date
JP5618413B2 (ja) 2014-11-05
WO2009048885A3 (en) 2009-12-30
IL204892B (en) 2018-02-28
AU2008311000B2 (en) 2013-12-19
AU2008311000A2 (en) 2010-06-03
CN101932725A (zh) 2010-12-29
CA2701923C (en) 2018-07-10
EP2207899A2 (en) 2010-07-21
EP2207899B1 (en) 2018-04-18
CA2701923A1 (en) 2009-04-16
EP2207899A4 (en) 2010-12-01
AU2008311000A1 (en) 2009-04-16
WO2009048885A2 (en) 2009-04-16
US9593329B2 (en) 2017-03-14
US20090275086A1 (en) 2009-11-05
DK2207899T3 (en) 2018-09-03
IL204892A0 (en) 2010-11-30
JP2010539994A (ja) 2010-12-24
MY161418A (en) 2017-04-14

Similar Documents

Publication Publication Date Title
MY161418A (en) Assembly of large nucleic acids
BRPI0914440A8 (pt) Método para produção biogênica de etanol e cianobactéria geneticamente modificada
EP2379748A4 (en) MULTIBASE RELEASE FOR LONG READINGS IN SEQUENCING BY SYNTHESIS PROTOCOLS
WO2013032850A3 (en) Compositions and methods for high fidelity assembly of nucleic acids
WO2011005786A3 (en) Compositions and methods for enhancing production of a biological product
MX343457B (es) Manipulación de la biosíntesis de fructosano y aumento de biomasa vegetal.
EA201390444A1 (ru) Производство бутанола из монооксида углерода рекомбинантным микроорганизмом
NZ597299A (en) Use of a cas gene in combination with CRISPR repeats for modulating resistance in a cell
MX2011004049A (es) Produccion de etanol en microorganismos.
EP4268832A3 (en) Nucleic acid molecules for pseudouridylation
WO2008005847A3 (en) Method of producing factor viii proteins by recombinant methods
MX2021000209A (es) Metodos y productos para transfeccion de celulas.
MX2009004659A (es) Procedimiento para la produccion biologica de 1,3-propanodiol a partir de glicerina con un alto rendimiento.
EA200870204A1 (ru) Системы и способы производства биотоплива и связанных с этим материалов
BRPI0506369A (pt) microorganismos, e, métodos para produzir um l-aminoácido
WO2008067070A3 (en) Compositions and methods for producing benzylisoquinolines alkaloids
MY164623A (en) Method for producing high amount of glycolic acid by fermentation
MY166543A (en) A microorganism for methionine production with enhanced glucose import
MX2012004579A (es) Recombinacion homologa en genoma nuclear de algas.
AR073602A1 (es) Metodo para producir un aditivo para la degradacion enzimatica de micotoxinas y aditivo y uso del mismo
WO2009073557A3 (en) Biological systems for production of commercially valuable compounds
BRPI0613543B8 (pt) Método para produção de 1,3-propanodiol usando glicerol bruto, um subproduto da produção de biodiesel
WO2008131191A8 (en) Nucleic acids hybridizable to micro rna and precursors thereof
BR112014001371A2 (pt) fermentação de glicerol para ácidos orgânicos
EA201200368A1 (ru) Способ ферментативного получения дикарбоновых кислот