MX2010003511A - Método optimizado y definido para el aislamiento y conservación de células precursoras del cordón umbilical humano. - Google Patents
Método optimizado y definido para el aislamiento y conservación de células precursoras del cordón umbilical humano.Info
- Publication number
- MX2010003511A MX2010003511A MX2010003511A MX2010003511A MX2010003511A MX 2010003511 A MX2010003511 A MX 2010003511A MX 2010003511 A MX2010003511 A MX 2010003511A MX 2010003511 A MX2010003511 A MX 2010003511A MX 2010003511 A MX2010003511 A MX 2010003511A
- Authority
- MX
- Mexico
- Prior art keywords
- cells
- umbilical cord
- optimized
- isolation
- precursor cells
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 6
- 239000002243 precursor Substances 0.000 title abstract 4
- 210000003954 umbilical cord Anatomy 0.000 title abstract 4
- 238000002955 isolation Methods 0.000 title abstract 2
- 238000004321 preservation Methods 0.000 title 1
- 238000011194 good manufacturing practice Methods 0.000 abstract 2
- 238000002659 cell therapy Methods 0.000 abstract 1
- 230000001413 cellular effect Effects 0.000 abstract 1
- 239000002537 cosmetic Substances 0.000 abstract 1
- 230000008014 freezing Effects 0.000 abstract 1
- 238000007710 freezing Methods 0.000 abstract 1
- 239000007787 solid Substances 0.000 abstract 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/10—Preservation of living parts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/44—Vessels; Vascular smooth muscle cells; Endothelial cells; Endothelial progenitor cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0604—Whole embryos; Culture medium therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0668—Mesenchymal stem cells from other natural sources
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Developmental Biology & Embryology (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Gynecology & Obstetrics (AREA)
- Cell Biology (AREA)
- Reproductive Health (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Rheumatology (AREA)
- Pregnancy & Childbirth (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Pharmacology & Pharmacy (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Vascular Medicine (AREA)
- Immunology (AREA)
- Virology (AREA)
- Epidemiology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Materials For Medical Uses (AREA)
- Cosmetics (AREA)
Abstract
La presente invención se refiere a un método optimizado y definido para el aislamiento y conservación de células precursoras a partir del cordón umbilical humano. Además de ser reproducible y 100% confiable en términos del número de muestras procesadas, el método resulta en un número alto y definido de células precursoras, la mayoría que se obtienen después de una fase de adhesión sencilla y de expansión/multiplicación ex vivo (otorgando así un fenotipo celular), en un marco de tiempo más corto que aquel que fue previamente descrito en el estado del arte. Con este método, es posible obtener en 9 días, después de congelamiento directo de una fracción celular, y después de una fase de expansión/multiplicación ex vivo (final de PO) de la mayoría de las células, alrededor de 8.6 (±0.1) x 105 células/gramo de cordón umbilical procesado. A su vez, las características de las células permiten por ejemplo, después de 35 días, obtener un promedio de 7.7 x 1015 células, con fenotipo del precursor, a partir del 100% de las muestras procesadas de cordón umbilical. El método, debido a que es sencillo, sólido y 100% confiable, se puede efectuar bajo buenas prácticas de manufactura (GMP) en laboratorios dedicados a la terapia celular en humanos. Además, el método tiene aplicaciones las áreas farmacéutica, de cosmética y biotecnología.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PT103843A PT103843B (pt) | 2007-10-04 | 2007-10-04 | Método de isolamento de células precursoras a partir do cordão umbilical humano |
| PCT/IB2008/054067 WO2009044379A2 (en) | 2007-10-04 | 2008-10-03 | Optimised and defined method for isolation and preservation of precursor cells from human umbilical cord |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2010003511A true MX2010003511A (es) | 2010-09-10 |
Family
ID=40456372
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2010003511A MX2010003511A (es) | 2007-10-04 | 2008-10-03 | Método optimizado y definido para el aislamiento y conservación de células precursoras del cordón umbilical humano. |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US9546353B2 (es) |
| EP (1) | EP2203555B1 (es) |
| JP (1) | JP5785394B2 (es) |
| KR (1) | KR20100087705A (es) |
| CN (1) | CN101868536B (es) |
| AU (1) | AU2008306442B2 (es) |
| BR (1) | BRPI0817522A2 (es) |
| CA (1) | CA2701907A1 (es) |
| EA (1) | EA025532B1 (es) |
| ES (1) | ES2582369T3 (es) |
| IL (1) | IL204788A (es) |
| MX (1) | MX2010003511A (es) |
| PT (1) | PT103843B (es) |
| WO (1) | WO2009044379A2 (es) |
| ZA (1) | ZA201002260B (es) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BR112012030678B1 (pt) | 2010-06-01 | 2021-10-19 | Auxocell Laboratories, INC | Método para purificação de células-tronco da geleia de wharton e método de cultivo de células-tronco mesenquimais |
| WO2012054501A1 (en) * | 2010-10-18 | 2012-04-26 | Sunshine Biotech Inc. | Human multipotent embryonic stem cell-like progenitor cells |
| TWI535377B (zh) * | 2011-09-01 | 2016-06-01 | Storage, culture and application of umbilical cord tissue and its derived cells | |
| DK3321355T3 (da) * | 2011-12-30 | 2021-11-08 | Amit Patel | Fremgangsmåder og sammensætninger til klinisk afledning af en allogen celle og terapeutiske anvendelser |
| CN102550543B (zh) * | 2012-02-10 | 2014-04-16 | 四川新生命干细胞科技股份有限公司 | 用于保存脐带组织的液体 |
| CN103461322B (zh) * | 2012-06-07 | 2016-08-03 | 西比曼生物科技（上海）有限公司 | 用于冻存复苏后的细胞的保存液 |
| EP2756754B1 (de) | 2013-01-17 | 2017-01-04 | Vita 34 Ag | Verfahren zur Behandlung von Nabelschnurgewebe, insbesondere im Zusammenhang mit der Konservierung des Gewebes |
| US20170119824A1 (en) * | 2014-05-24 | 2017-05-04 | Neelam Krishnan Venkataramanaa | A method for the treatment of glioblastoma with wharton jelly-mesenchymal stem cells (wj-msc) derived from human umbilical cord |
| EP3215224A4 (en) * | 2014-11-05 | 2018-06-27 | Tissuetech, Inc. | Compositions and method for promoting nerve growth and regeneration |
| WO2017004460A1 (en) * | 2015-06-30 | 2017-01-05 | Surgenex, LLC | Compositions and methods for flowable allograft tissue |
| WO2017096611A1 (zh) * | 2015-12-11 | 2017-06-15 | 郭镭 | 一种从脐带华通氏胶组织中分离培养脐带间充质干细胞的方法 |
| US20210047621A1 (en) | 2018-03-12 | 2021-02-18 | Universidade Do Porto | Compositions for use in the treatment of musculoskeletal conditions and methods for producing the same leveraging the synergistic activity of two different types of mesenchymal stromal/stem cells |
| CN112342191A (zh) * | 2019-08-07 | 2021-02-09 | 路春光 | hMSC生产试剂盒开发和质量管理标准优化和体系建立 |
| CN111893092A (zh) * | 2020-06-28 | 2020-11-06 | 国大生命科学产业集团(深圳)有限公司 | 一种人脐带来源的间充质干细胞及其制备方法 |
| CN113025567A (zh) * | 2021-03-31 | 2021-06-25 | 中国人民解放军陆军特色医学中心 | 一种椎间盘单细胞的分离方法 |
| DE102021116694B4 (de) * | 2021-06-29 | 2023-03-09 | Promocell Gmbh | Wässrige Lösung zur Zellkonservierung |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AT402890B (de) * | 1994-03-16 | 1997-09-25 | Berbi Gmbh | Verwendung von notoginsenosid r1 zur herstellung von arzneimitteln |
| JPWO2003080822A1 (ja) * | 2002-03-27 | 2005-07-28 | ニプロ株式会社 | 胎盤由来の間葉系細胞およびその医学的用途 |
| KR20080097190A (ko) * | 2005-12-29 | 2008-11-04 | 안트로제네시스 코포레이션 | 태반 줄기세포의 수집과 보존을 위한 개선된 조성물과 이조성물의 이용 방법 |
| CA2669589C (en) * | 2006-11-13 | 2016-01-12 | Ethicon, Incorporated | In vitro expansion of postpartum-derived cells using microcarriers |
-
2007
- 2007-10-04 PT PT103843A patent/PT103843B/pt active IP Right Grant
-
2008
- 2008-10-03 KR KR1020107009850A patent/KR20100087705A/ko not_active Ceased
- 2008-10-03 ES ES08807881.1T patent/ES2582369T3/es active Active
- 2008-10-03 EP EP08807881.1A patent/EP2203555B1/en not_active Not-in-force
- 2008-10-03 US US12/680,604 patent/US9546353B2/en not_active Expired - Fee Related
- 2008-10-03 AU AU2008306442A patent/AU2008306442B2/en not_active Ceased
- 2008-10-03 EA EA201000425A patent/EA025532B1/ru not_active IP Right Cessation
- 2008-10-03 WO PCT/IB2008/054067 patent/WO2009044379A2/en not_active Ceased
- 2008-10-03 JP JP2010527592A patent/JP5785394B2/ja not_active Expired - Fee Related
- 2008-10-03 BR BRPI0817522-5A2A patent/BRPI0817522A2/pt not_active Application Discontinuation
- 2008-10-03 CA CA2701907A patent/CA2701907A1/en not_active Abandoned
- 2008-10-03 CN CN200880114557.0A patent/CN101868536B/zh not_active Expired - Fee Related
- 2008-10-03 MX MX2010003511A patent/MX2010003511A/es active IP Right Grant
-
2010
- 2010-03-28 IL IL204788A patent/IL204788A/en not_active IP Right Cessation
- 2010-03-30 ZA ZA2010/02260A patent/ZA201002260B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EA201000425A1 (ru) | 2010-10-29 |
| IL204788A0 (en) | 2010-12-30 |
| EA025532B1 (ru) | 2017-01-30 |
| CA2701907A1 (en) | 2009-04-09 |
| PT103843B (pt) | 2008-08-12 |
| EP2203555A2 (en) | 2010-07-07 |
| AU2008306442B2 (en) | 2014-12-04 |
| ES2582369T3 (es) | 2016-09-12 |
| AU2008306442A1 (en) | 2009-04-09 |
| BRPI0817522A2 (pt) | 2014-10-14 |
| JP2010539970A (ja) | 2010-12-24 |
| IL204788A (en) | 2013-07-31 |
| CN101868536B (zh) | 2015-03-25 |
| JP5785394B2 (ja) | 2015-09-30 |
| US20100216237A1 (en) | 2010-08-26 |
| US9546353B2 (en) | 2017-01-17 |
| WO2009044379A3 (en) | 2009-05-22 |
| CN101868536A (zh) | 2010-10-20 |
| KR20100087705A (ko) | 2010-08-05 |
| WO2009044379A2 (en) | 2009-04-09 |
| PT103843A (pt) | 2008-01-09 |
| EP2203555B1 (en) | 2016-04-13 |
| ZA201002260B (en) | 2011-06-29 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| HH | Correction or change in general | ||
| FG | Grant or registration |