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MX2009006681A - Composiciones y metodos para la expresion de acidos nucleicos. - Google Patents

Composiciones y metodos para la expresion de acidos nucleicos.

Info

Publication number
MX2009006681A
MX2009006681A MX2009006681A MX2009006681A MX2009006681A MX 2009006681 A MX2009006681 A MX 2009006681A MX 2009006681 A MX2009006681 A MX 2009006681A MX 2009006681 A MX2009006681 A MX 2009006681A MX 2009006681 A MX2009006681 A MX 2009006681A
Authority
MX
Mexico
Prior art keywords
compositions
methods
expression
nucleic acids
transposon
Prior art date
Application number
MX2009006681A
Other languages
English (en)
Inventor
David P Davis
Daniel C Gray
Zhenyu Gu
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of MX2009006681A publication Critical patent/MX2009006681A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/635—Externally inducible repressor mediated regulation of gene expression, e.g. tetR inducible by tetracyline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1135—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against oncogenes or tumor suppressor genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1136—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against growth factors, growth regulators, cytokines, lymphokines or hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/50—Methods for regulating/modulating their activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Endocrinology (AREA)
  • Oncology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Se proporcionan aquí composiciones y métodos para la expresión de ácidos nucleicos. Las composiciones y métodos se proporcionan también aquí para la expresión inducible de ácidos nucleicos en células transgénicas y animales usando estructuras de ácidos nucleicos basadas en transposón. Las composiciones y los métodos se proporcionan también aquí para la modulación de la expresión de un gen endógeno.
MX2009006681A 2006-12-21 2007-12-04 Composiciones y metodos para la expresion de acidos nucleicos. MX2009006681A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US87139006P 2006-12-21 2006-12-21
PCT/US2007/086417 WO2008079608A1 (en) 2006-12-21 2007-12-04 Compositions and methods for the expression of nucleic acids

Publications (1)

Publication Number Publication Date
MX2009006681A true MX2009006681A (es) 2009-07-29

Family

ID=39368542

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2009006681A MX2009006681A (es) 2006-12-21 2007-12-04 Composiciones y metodos para la expresion de acidos nucleicos.

Country Status (7)

Country Link
US (1) US20100077495A1 (es)
EP (1) EP2097530A1 (es)
JP (1) JP2010512794A (es)
AU (1) AU2007337263A1 (es)
CA (1) CA2672203A1 (es)
MX (1) MX2009006681A (es)
WO (1) WO2008079608A1 (es)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20110097798A1 (en) * 2008-06-30 2011-04-28 Atgcell Inc. Mammalian cell expression vectors and utilization
WO2010065550A2 (en) * 2008-12-01 2010-06-10 Board Of Regents University Of Texas System Production and use of rat spermatogonial stem cell lines
WO2010083841A2 (en) * 2009-01-23 2010-07-29 Nsgene A/S Improved cell lines and their use in encapsulated cell biodelivery
JP5447803B2 (ja) * 2009-07-02 2014-03-19 独立行政法人産業技術総合研究所 鳥類卵管細胞を用いた抗体製造方法
EP2653541B1 (en) * 2010-12-15 2017-11-08 Kyowa Hakko Kirin Co., Ltd. Method for producing proteins
DK3359671T3 (da) 2015-10-08 2021-09-20 Dna Twopointo Inc Dna-vektorer, transposoner og transposaser til eukaryot genommodifikation
US11466290B2 (en) * 2016-01-26 2022-10-11 Cedars-Sinai Medical Center Systems and methods for in vivo dual recombinase-mediated cassette exchange (dRMCE) and disease models thereof
CN110402305B (zh) * 2016-11-30 2023-07-21 北京复昇生物科技有限公司 一种crispr文库筛选的方法
WO2019178550A1 (en) 2018-03-16 2019-09-19 Cedars-Sinai Medical Center Methods and compositions for inducible expression of neurotrophic factors
US20190300870A1 (en) * 2018-04-03 2019-10-03 The Broad Institute, Inc. Target recognition motifs and uses thereof
US20230203530A1 (en) * 2020-02-19 2023-06-29 WuXi Biologics Ireland Limited Enhanced expression system and methods of use thereof
CN111996214A (zh) * 2020-07-29 2020-11-27 温州医科大学 一种人多能干细胞中可诱导高效表达基因体系的建立及应用
WO2025250465A1 (en) * 2024-05-28 2025-12-04 Nanjing Probio Biotech Co., Ltd. Vector composition and use thereof

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6962810B2 (en) * 2000-10-31 2005-11-08 University Of Notre Dame Du Lac Methods and compositions for transposition using minimal segments of the eukaryotic transformation vector piggyBac
GB2404382B (en) * 2003-07-28 2008-01-30 Oxitec Ltd Pest control
EP1627563A1 (en) * 2004-08-10 2006-02-22 Academisch Medisch Centrum bij de Universiteit van Amsterdam Means and methods for producing a stabilized cell of interest
EP1690942A1 (en) * 2005-02-11 2006-08-16 Friedrich-Alexander-Universität Erlangen-Nürnberg Tetracycline-controlled insertion element for systematic mutagenesis
WO2007004062A2 (en) * 2005-05-31 2007-01-11 Centre National De La Recherche Scientifique Tetracycline-dependent regulation of rna interference
WO2006131543A1 (en) * 2005-06-09 2006-12-14 Artemis Pharmaceuticals Gmbh Shrna and sirna expression in a living organism under control op a codon-optimized tetracycline repressor gene
US20080220471A1 (en) * 2005-07-27 2008-09-11 Genentech, Inc. Vectors and Methods Using Same

Also Published As

Publication number Publication date
US20100077495A1 (en) 2010-03-25
AU2007337263A1 (en) 2008-07-03
WO2008079608A1 (en) 2008-07-03
EP2097530A1 (en) 2009-09-09
AU2007337263A2 (en) 2009-08-20
JP2010512794A (ja) 2010-04-30
CA2672203A1 (en) 2008-07-03

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