MX2008011324A - Process for evaluating the antioxidant ability in aqueous extracts of food. - Google Patents
Process for evaluating the antioxidant ability in aqueous extracts of food.Info
- Publication number
- MX2008011324A MX2008011324A MX2008011324A MX2008011324A MX2008011324A MX 2008011324 A MX2008011324 A MX 2008011324A MX 2008011324 A MX2008011324 A MX 2008011324A MX 2008011324 A MX2008011324 A MX 2008011324A MX 2008011324 A MX2008011324 A MX 2008011324A
- Authority
- MX
- Mexico
- Prior art keywords
- abts
- antioxidant
- food
- lacasse
- evaluating
- Prior art date
Links
- 239000003963 antioxidant agent Substances 0.000 title abstract 6
- 230000003078 antioxidant effect Effects 0.000 title abstract 6
- 238000000034 method Methods 0.000 title abstract 3
- 239000006286 aqueous extract Substances 0.000 title abstract 2
- ZTOJFFHGPLIVKC-YAFCTCPESA-N (2e)-3-ethyl-2-[(z)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound S\1C2=CC(S(O)(=O)=O)=CC=C2N(CC)C/1=N/N=C1/SC2=CC(S(O)(=O)=O)=CC=C2N1CC ZTOJFFHGPLIVKC-YAFCTCPESA-N 0.000 abstract 4
- AEMRFAOFKBGASW-UHFFFAOYSA-N Glycolic acid Chemical compound OCC(O)=O AEMRFAOFKBGASW-UHFFFAOYSA-N 0.000 abstract 4
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 abstract 2
- ZTOJFFHGPLIVKC-UHFFFAOYSA-N 3-ethyl-2-[(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound S1C2=CC(S(O)(=O)=O)=CC=C2N(CC)C1=NN=C1SC2=CC(S(O)(=O)=O)=CC=C2N1CC ZTOJFFHGPLIVKC-UHFFFAOYSA-N 0.000 abstract 1
- 102000030523 Catechol oxidase Human genes 0.000 abstract 1
- 108010031396 Catechol oxidase Proteins 0.000 abstract 1
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 abstract 1
- 241000208227 Toxicodendron vernicifluum Species 0.000 abstract 1
- 239000008351 acetate buffer Substances 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 239000003593 chromogenic compound Substances 0.000 abstract 1
- 229910001882 dioxygen Inorganic materials 0.000 abstract 1
- 229940074391 gallic acid Drugs 0.000 abstract 1
- 235000004515 gallic acid Nutrition 0.000 abstract 1
- 230000002401 inhibitory effect Effects 0.000 abstract 1
- 230000005764 inhibitory process Effects 0.000 abstract 1
- 238000011002 quantification Methods 0.000 abstract 1
- 239000011541 reaction mixture Substances 0.000 abstract 1
Landscapes
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Abstract
The present invention relates to the development of a quick, easy and reproducible method for evaluating the antioxidant ability in food samples, where a system of the model ABTS/lacasse was used for that purpose, the system including the chromogenic compound 2,2'-azino-bis(3-ethylbenz- thiazoline-6-sulfonic acid), (ABTS) and lacasse , which is a polyphenol oxidase for generating ABTS+ free radicals in the presence of molecular oxygen. The method involves preparing a pattern curve where a standard antioxidant, preferably glycolic acid, is used. The reaction mixture of 1 ml includes: Denilite IISTM or Rhus vernicifera lacasse, ABTS 0.05 mM in an acetate buffer of 0.1 M with pH 4 and glycolic acid at different concentrations and at a temperature of 25°C. The lag time for inhibiting the formation of the ABTS radical is determined by following the reaction in a spectrophotometer at 728 nm. An antioxidant concentration vs ABTSò+ inhibition lag time graphic is subsequently built. The quan tification of the sample is performed by substituting the standard antioxidant by a suitable aliquot from the aqueous extract of the food to be assessed. The result is expressed in equivalent pg of food gallic acid/g or any other antioxidant equivalent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MX2008011324A MX2008011324A (en) | 2008-09-04 | 2008-09-04 | Process for evaluating the antioxidant ability in aqueous extracts of food. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MX2008011324A MX2008011324A (en) | 2008-09-04 | 2008-09-04 | Process for evaluating the antioxidant ability in aqueous extracts of food. |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2008011324A true MX2008011324A (en) | 2010-03-04 |
Family
ID=42269807
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2008011324A MX2008011324A (en) | 2008-09-04 | 2008-09-04 | Process for evaluating the antioxidant ability in aqueous extracts of food. |
Country Status (1)
| Country | Link |
|---|---|
| MX (1) | MX2008011324A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013058676A1 (en) | 2011-10-21 | 2013-04-25 | ОБЩЕСТВО С ОГРАНИЧЕННОЙ ОТВЕТСТВЕННОСТЬЮ "ДИСофт" | Method for determining the quantity and/or activity of antioxidants |
| CN105241965A (en) * | 2014-07-09 | 2016-01-13 | 西南大学 | Method of on-line quickly detecting total anti-oxidizing property of sample |
| ES2590351A1 (en) * | 2015-05-18 | 2016-11-21 | Electrochemistry For Lightweight & Integrated Analytical Solutions Elias Sl | Synthesis and storage procedure for the dissolution of radical abts and its use for the measurement of antioxidant capacity (Machine-translation by Google Translate, not legally binding) |
-
2008
- 2008-09-04 MX MX2008011324A patent/MX2008011324A/en active IP Right Grant
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013058676A1 (en) | 2011-10-21 | 2013-04-25 | ОБЩЕСТВО С ОГРАНИЧЕННОЙ ОТВЕТСТВЕННОСТЬЮ "ДИСофт" | Method for determining the quantity and/or activity of antioxidants |
| CN105241965A (en) * | 2014-07-09 | 2016-01-13 | 西南大学 | Method of on-line quickly detecting total anti-oxidizing property of sample |
| ES2590351A1 (en) * | 2015-05-18 | 2016-11-21 | Electrochemistry For Lightweight & Integrated Analytical Solutions Elias Sl | Synthesis and storage procedure for the dissolution of radical abts and its use for the measurement of antioxidant capacity (Machine-translation by Google Translate, not legally binding) |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG | Grant or registration |