MX2008010413A

MX2008010413A - Nucleic acid constructs and methods for producing altered seed oil compositions.

Info

Publication number: MX2008010413A
Application number: MX2008010413A
Authority: MX
Inventors: Neal A Bringe; Toni Voelker; Joanne J Fillatti; Tim Ulmasov
Original assignee: Monsanto Technology Llc
Priority date: 2006-02-13
Filing date: 2007-02-12
Publication date: 2009-01-09
Also published as: RU2008136856A; RU2392795C2; JP2009526550A; JP5242418B2; BRPI0707755A2

Abstract

The present invention is in the field of plant genetics and provides recombinant nucleic acid molecules, constructs, and other agents associated with the coordinate manipulation of multiple genes in the fatty acid synthesis pathway. In particular, the agents of the present invention are associated with the simultaneous enhanced expression of certain genes in the fatty acid synthesis pathway and suppressed expression of certain other genes in the same pathway. Also provided are plants incorporating such agents, and in particular plants incorporating such constructs where the plants exhibit altered seed oil compositions, e.g. soybean plants bearing see, wherein the seed exhibit an oil composition which comprises 42-85% oleic acid and 1.5-8% saturated fatty acids. The genes to be suppressed are selected from the group consisting of FAD2, FAD3, and FATB, and the genes whose expression is to be enhanced are selected from the group consisting of beta-ketoacyl-ACP synthase I, beta-ketoacyl-ACP synthase IV, and delta-9 desaturase.

Description

NUCLEIC ACID CONSTRUCTS AND METHODS FOR THE PRODUCTION OF SEED OIL COMPOSITIONS ALTERED CROSS REFERENCE TO RELATED REQUESTS This application claims the benefit under 35 U.S.C. § 1 19 (e) of the Provisional Application of E.U.A. No. 60 / 772,614, whose title is "Modified Gene Silencing," filed on February 13, 2006; Provisional Application No. 60/781, 519 entitled "Soybean Seed and Oil Compositions and Method for Making Same," filed on March 10, 2006 and the application of E.U.A. No. 1 1 / 376,328, whose title is "Nucleic Acid Constructs and Methods for Producing Altered Seed Oil Compositions" filed on March 16, 2006.

INCORPORATION OF THE LIST OF SEQUENCES A paper copy of the sequence listing and a computer-readable form of the flexible disk sequence listing, which contains the name of the "Omni2 AS FILED.txt" tab, which is 60,690 bytes in size (as measured in MS- DOS) and that is registered on September 25, 2003 and presented in the EUA Application No. 10 / 669,888, are incorporated herein for reference. A paper copy of the sequence listing and a legible form computer of the list of sequence in flexible disc, that contains the name of ficha "OmniChild.txt", that has a size of 61, 434 bytes of size (as measured in MS-DOS) and that registers the 15 of March of 2006 is incorporated here for reference.

FIELD OF THE INVENTION The present invention relates to recombinant nucleic acid molecules, constructs, and other agents associated with the coordinated manipulation of multiple genes in the path of fatty acid synthesis. In particular, the agents of the present are associated with the simultaneous increased expression of certain genes in the pathway of fatty acid synthesis and suppressed expression of certain different genes in the same path. The present invention also relates to plants that incorporate said agents, and in particular to plants that incorporate said constructs where the plants exhibit altered seed oil compositions.

BACKGROUND OF THE INVENTION Vegetable oils are used in a variety of applications.

Novel vegetable oil compositions and improved methods are needed to obtain oily compositions, from bio-synthetic or natural plant sources. Depending on the use of the intended oil, several different fatty acid compositions are desired. Plants, especially species that synthesize large quantities of oils in seeds, are an important source of oils for edible and industrial uses. Seed oils are composed almost entirely of triacylglycerols in which the fatty acids are esterified to the three hydroxyl groups of glycerol. Soybean oil typically contains approximately 16-20% saturated fatty acids: 13-16% palmitate and 3-4% stearate. See generally Gunstone et al., The lipid Handbook, Chapman & Hall, London (1994). Soybean oils have been modified by various breeding methods to create benefits for specific markets. However, soybean oil is not available that is widely beneficial to most soybean oil users such as consumers of salad oil, cooking oil and frying oil, and in commercial markets such as in markets that use biodiesel or lubricating oil. Soybean oils are either very expensive or lack an important qualitative food property such as oxidative stability, good taste of fried foods or saturated fat content, or a property of important biodiesel such as appropriate nitric oxide emitters or tolerance to Cold or cold flow. Higher plants synthesize fatty acids via a common metabolic pathway - the pathway of fatty acid synthetase (FAS), which is located in the plastids. Β-ketoacyl-ACP synthases are important important frequency limiting enzymes in cell FAS vegetables and they exist in several versions. The ß-ketoacyl-ACP synthase I catalyses the elongation of the chain to plamitoyl-ACP (C16: 0), while the ß-ketoacyl-ACP synthase II catalyzes the chain elongation to stearoyl.ACP (C18: 0). β-ketoacyl-ACP synthase IV is a variant of β-ketoacyl-ACP synthase II, and can also catalyze chain elongation at 18: 0-ACP. In soybeans, the main products of FAS are 16: 0-ACP and 18: 0-ACP. The desaturation of 18: 0-ACP to form 18: 1 -ACP is catalyzed by a soluble delta-9 desaturase located in plastid (also referred to as "stearoyl-ACP desaturase"). See Voelker et al., 52 Annu. Rev. Plant Physiol. Plant Mol. Biol. 335-61 (2001). The FAS plastidial and delta-9 desaturase products, 16: 0-ACP, 18: 0-ACP, and 18: 1 -ACP, are hydrolyzed by specific thioesterases (FAT). Plant thioesterases can be classified into two gene families based on sequence homology and substrate preference. The first family, FATA, includes acyl-ACP long-chain thioesterases that have activity primarily in 18: 1 -ACP. Enzymes of the second family, FATB, commonly use 16: 0-ACP (palmitoyl-ACP), 18: 0-ACP (stearoyl-ACP), and 18: 0-ACP (oleoyl-ACP). Said thioesterases have an important function in the determination of chain length during the biosynthesis of de novo fatty acid in plants, and thus these enzymes are useful in providing various modifications of fatty acyl compositions, particularly with respect to the proportions relative of various fatty acyl groups that are present in storage seed oils.

The products of the FATA and FATB reactions, the free fatty acids, leave the plastids and are converted to their respective acyl-CoA esters. Acyl-CoAs are substrates for the pathway of lipid biosynthesis (Kennedy Pathway), which is located in the endoplasmic reticulum (ER). This trajectory is responsible for the lipid formation of the membrane as well as the biosynthesis of triacylglycerols, which constitute the oil of the seed. In the ER there are additional membrane binding desaturases, which can further desaturate 18: 1 to polyunsaturated fatty acids. A delta-12 desaturase (FAD2) catalyzes the insertion of a double bond in 18: 1, forming linoleic acid (18: 2). A delta-15 desaturase (FAD3) catalyzes the insertion of a double bond in 18: 2, forming linoleic acid (18: 3). Many complex biochemical trajectories have now been genetically engineered, usually through the suppression or over-expression of single genes. Further exploitation of the plant genetic manipulation potential will require the coordinated manipulation of multiple genes in one path. A number of methods have been used to combine transgenes in a plant - including sexual crossbreeding, retransformation, co-transformation, and the use of linked transgenes. A chimeric transgene with linked partial gene sequences can be used to coordinately suppress numerous plant endogenous genes. Constructs modeled on viral polyproteins can be used to simultaneously introduce multiple coding genes into plant cells. For a review, see Halpin et al., Plant Mol. Biol. 47: 295-310 (2001).

Thus, a desired plant phenotype may require the expression of one or more genes and the concurrent reduction of expression of another gene or genes. Thus, there is a need to simultaneously over-express one or more genes and suppressors, or sub-regulate, the expression of one other gene or genes in plants using a simple transgenic construct.

BRIEF DESCRIPTION OF THE INVENTION The present invention provides a recombinant nucleic acid molecule or molecules, which when introduced into a cell or organism are capable of suppressing, at least partially reducing, reducing, substantially reducing, or effectively eliminating the expression of at least one or more FAD2 RNA., Endogenous FAD3 or FATB while at the same time co-expressing, simultaneously co-expressing, or coordinately producing one or more RNA or transcribed proteins of a gene encoding beta-ketoacyl-ACP synthase I, beta-ketoacyl-ACP synthase IV, delta-9 desaturase, or CP4 EPSPS. The present invention also provides plant cells and plants transformed with the same molecule or nucleic acid molecules, and seeds, oil and other products produced from the transformed plants. Also provided by the present invention is a recombinant nucleic acid molecule comprising a first set of DNA sequences that are capable, when expressed in a host cell, of suppressing the endogenous expression of at least one, preferably two, genes selected from the group consisting of FAD2, FAD3, and FATB genes; and a second set of DNA sequences which is capable, when expressed in a host cell, of increasing the endogenous expression of at least one gene selected from the group consisting of a beta-ketoacyl-ACP synthase I gene, a beta-gene. ketoacyl-ACP synthase IV, a delta-9 desaturase gene, and CP4 EPSPS. Further provided by the present invention is a recombinant nucleic acid molecule comprising a first set of DNA sequences which is capable, when expressed in a host cell, of forming a dsRNA construct and suppressing the endogenous expression of at least one , preferably two, genes selected from the group consisting of FAD2, FAD3, and FATB genes, wherein the first set of DNA sequences comprises a first non-coding sequence that expresses a first RNA sequence exhibiting at least 90% identity to a non-coding region of a FAD2 gene, a first antisense sequence expressing a first antisense RNA sequence capable of forming a double stranded RNA molecule with the first RNA sequence, a second non-coding sequence expressing a second RNA sequence exhibiting at least 90% identity to a non-coding region of a FATB gene, and a second antisense sequence which e expresses a second antisense RNA sequence capable of forming a double stranded RNA molecule with the second RNA sequence; and a second set of DNA sequences that is capable, when expresses in a host cell, of increasing the endogenous expression of at least one gene selected from the group consisting of a beta-ketoacyl-ACP synthase I gene, a beta-ketoacetyl-ACP IV gene, a delta-9 desaturase gene, and CP4 EPSPS. The present invention provides methods of transforming plants with these recombinant nucleic acid molecules. The methods include a method for the production of a transformed plant having a seed with an increased oleic acid content, reduced saturated fatty acid content, and reduced polyunsaturated fatty acid content, comprising (A) the transformation of a plant cell with a recombinant nucleic acid molecule comprising a first set of DNA sequences which is capable, when expressed in a host cell, of suppressing the endogenous expression of at least one, preferably two, genes selected from the group consisting of FAD2 genes , FAD3, and FATB, and a second set of DNA sequences that is capable, when expressed in a host cell, of increasing the endogenous expression of at least one gene selected from the group consisting of a beta-ketoacetyl-ACP synthase gene I, a beta-ketoacyl-ACP synthase IV gene, a delta-9 desaturase gene, and CP4 EPSPS; and (B) the growth of the transformed plant, where the transformed plant produces seed with an increased oleic acid content, reduced saturated fatty acid content, and polyunsaturated fatty acid content in relation to the seed of a plant having a bottom genetic but lacking nucleic acid molecule recombinant. In addition methods are provided for the transformation of plant cells with the recombinant nucleic acid molecules. The methods include a method of altering the oily composition of a plant cell comprising (A) transforming a plant cell with a recombinant nucleic acid molecule comprising a first set of DNA sequences that is capable, when expressed in a host cell, of suppressing the endogenous expression of at least one, preferably two, genes selected from the group consisting of FAD2, FAD3, and FATB genes, and a second set of DNA sequences that is capable, when expressed in a host cell , of increasing the endogenous expression of at least one gene selected from the group consisting of a beta-ketoacetyl-ACP synthase I gene, a beta-ketoacyl-ACP synthase IV gene, a delta-9 desaturase gene, and CP4 EPSPS; and (B) the growth of the plant cell under conditions where the transcription of the first set of DNA sequences and the second set of DNA sequences is initiated, where the oily composition is altered in relation to a plant cell with a similar genetic background but lacking the recombinant nucleic acid molecule. The present invention also provides a transformed plant comprising a nucleic acid molecule comprising a recombinant nucleic acid molecule comprising a first set of DNA sequences that is capable, when expressed in a cell host, to suppress the endogenous expression of at least one, preferably two, genes selected from the group consisting of FAD2, FAD3, and FATB genes, and a second set of DNA sequences that is capable, when expressed in a host cell, of increasing the endogenous expression of at least one gene selected from the group consisting of a beta-ketoacetyl-ACP synthase I gene, a beta-ketoacyl-ACP synthase IV gene, a delta-9 desaturase gene, and CP4 EPSPS. Also provided by the present invention is a processed soybean plant having seed, wherein the seed exhibits an oily composition comprising 55 to 80% by weight of oleic acid, 10 to 40% by weight of linoleic acid, 6% or less in weight of linoleic acid, and 2 to 8% by weight of saturated fatty acids and raw material, plant parts, and seed derived from the plant. In another embodiment, the present invention provides and a transformed soybean plant having seed, wherein the seed exhibits an oily composition comprising about 65-80% oleic acid, about 3-8% saturated, and about 12-32% of poly-unsaturated. Also included is the raw material, plant parts, and seed derived from said plant. In another embodiment, the present invention provides a transformed soybean plant having seed, wherein the seed exhibits an oily composition comprising approximately 65-80% oleic acid, approximately 2-3.5% saturates, and approximately 16.5-33% polyunsaturated Raw material, plant parts, and seed derived from said plant are also included.

The present invention provides a soybean seed that exhibits an oily composition comprising 55 to 80% by weight of oleic acid, 10 to 40% by weight of linoleic acid, 6% or less by weight of linoleic acid, and 2 to 8% by weight of saturated fatty acids and also provides a soybean seed that exhibits an oily composition comprising about 65-80% by weight of oleic acid, 10-30% by weight of linoleic acid, 6% or less by weight of linoleic acid , and 2 to 8% by weight of saturated fatty acids. In another embodiment, the present invention provides a soybean seed that exhibits an oily composition comprising about 65-80% oleic acid, about 3-8% saturated, and about 12-32% polyunsaturated. In another embodiment, the present invention provides a soybean seed that exhibits an oily composition comprising about 65-80% oleic acid, about 2-3.5% saturates, and about 16.5-33% polyunsaturates. Also provided by the present invention are the soy foods comprising an oily composition comprising 69 to 73% by weight of oleic acid, 21 to 24% by weight of linoleic acid, 0.5 to 3% by weight of linoleic acid, and -3% by weight of saturated fatty acids. The crude soybean oil provided by the present invention exhibits 55 to 80% by weight of oleic acid, 10 to 40% by weight of linoleic acid, 6% or less by weight of linoleic acid, and 2 to 8% by weight of saturated fatty acids. Another crude soybean oil provided by the present invention exhibits an oily composition comprising 65 to 80% by weight of oleic acid, 10 to 30% by weight of Iinoleic acid, 6% or less by weight of Iinoleic acid, and 2 to 8% by weight of saturated fatty acids. In another embodiment, the crude soybean oil provided by the present invention exhibits an oily composition comprising about 65-80% by weight oleic acid, about 3-8% saturated, and about 12-32% polyunsaturated. In another embodiment, the raw soybean oil provided by the present invention exhibits an oily composition comprising about 65-80% by weight oleic acid, about 2-3.5% saturated, and about 16.5-33% polyunsaturated. The present invention also provides a soybean seed that exhibits an oily composition comprising about 42% to about 85% by weight of oleic acid and about 8% to about 1.5% by weight of saturated fatty acids. In another modality, a soybean seed of the present invention exhibits an oily composition comprising about 42% to about 85% by weight of oleic acid, about 8% to about 1.5% by weight of saturated fatty acids, less than about 35% by weight. Iinoleic acid weight, wherein a combined amount of oleic acid and linoleic acid is about 65% to about 90% by weight of the total oily composition; and the seed has a recombinant nucleic acid molecule with a DNA sequence having an FAD2-1 intron fragment between about 50 and approximately 400 contiguous nucleotides in length, a FATB3 'UTR, and a FATB 5' UTR, a heterologous beta-ketoacyl-ACP synthase V, and heterologous delta-9 desaturase in a host cell. A soybean seed of the present invention can exhibit an oil composition comprising about 50% to about 80% by weight of oleic acid, about 8% to about 1.5% by weight of saturated fatty acids, about 2% to about 45% by weight of linoleic acid, about 4% to about 14% by weight of linoleic acid, wherein a combined amount of oleic acid and linoleic acid is about 65% to about 90% by weight of the total oily composition, and the seed comprises a recombinant nucleic acid molecule comprising a DNA sequence comprising an FAD2-1 intron fragment that is between about 50 and about 400 contiguous nucleotides in length, a FATB CTP coding region, and 42 contiguous nucleotides of a FATB 5 'UTR. In another embodiment, a soybean may comprise a recombinant nucleic acid molecule comprising a DNA sequence that suppresses the endogenous expression of FAD2 and FATB, wherein the seed exhibits an oily composition comprising 46 to 75% by weight of acid oleic, 1.5 to 8.5% by weight of saturated fatty acids, 2.5 to 38% by weight of linoleic acid, and 4.5 to 17.5% by weight of linoleic acid. The present invention also includes a method for reducing the amount of FAD2 gene deletion with respect to the amount of FAD2 gene deletion obtained by the expression of an RNAids construct having a recombinant FAD2 sequence consisting of a complete FAD2 intron or a complete FAD2 UTR per; i) expression of a recombinant FAD2 sequence in a plant cell, wherein the recombinant FAD2 sequence is derived from an endogenous FAD2 gene in a plant cell and the recombinant FAD2 sequence consists of an FAD2 intron fragment or a FAD2 UTR fragment; yi) deletion of an endogenous FAD2 gene with the recombinant FAD2 sequence, wherein the amount of the FAD2 gene deletion is less than the amount of gene expression obtained by the expression of an RNAids construct having a recombinant FAD2 sequence consisting of the full length of an FAD2 intron or the full length of an FAD2 UTR. Also provided by the present invention are methods for altering the oily composition of a plant cell by: transforming a plant cell with a recombinant FAD2 sequence derived from a part of an endogenous FAD2 gene. The recombinant FAD2 sequence consists of an FAD2 intron fragment or a FAD2 UTR fragment; and the growth of the plant cell under conditions where the transcription of the recombinant sequence FAD2 is initiated, whereby the oily composition is altered in relation to a plant cell with a similar genetic background but lacking the recombinant FAD2 sequence. In another modality, a method for increase the content of oleic acid and the reduction of saturated fatty acid content in a plant seed by: i) shortening the length of a first recombinant FAD2 sequence up to the amount of gene deletion FAD2 of a plant transformed with the first recombinant sequence FAD2 is at least partially reduced in relation to the amount of gene deletion FAD2 in a plant cell comprising a similar genetic background and a second recombinant FAD2 sequence, wherein the second FAD2 recombinant sequence consists of more endogenous FAD2 sequences than the first recombinant FAD2 sequence; ii) the expression of a recombinant FATB sequence capable of at least partially reducing FATB gene expression in a plant cell in relation to the suppression of FATB in a plant cell with a similar genetic background but without the recombinant FATB sequence; iii) the growth of a plant with a recombinant nucleic acid molecule comprising the first recombinant FAD2 sequence and the recombinant FATB sequence; and iv) cultivating a plant that produces seed with a reduced saturated fatty acid content relative to the seed of a plant that has a similar genetic background but lacks the first recombinant FAD2 sequence and the recombinant FATB sequence. In yet another embodiment, the present invention includes a method for the production of a transformed plant having seed with a reduced saturated fatty acid content by: transforming a plant cell with a recombinant nucleic acid molecule comprising a recombinant DNA sequence that suppresses the endogenous expression of FAD2 and FATB, where the recombinant DNA sequence has a recombinant FAD2 nucleic acid sequence and recombinant FATB, wherein the FAD2 sequence consists of less than the complete sequence of an FAD2 intron; and the growth of the transformed plant, wherein the transformed plant produces seed with a reduced saturated fatty acid content in relation to the seed of a plant that has a similar genetic background but lacks the recombinant DNA sequence. In another embodiment, the present invention relates to a method of modulating the fatty acid composition of oil from a seed of a temperate oilseed crop by isolating a genetic element of at least 40 nucleotides that is capable of suppressing the expression of an endogenous gene in the path of fatty acid synthesis; generate more than one shortened fragment of the genetic element; introducing each of the more than one shortened fragment into a plant cell of the temperate oil seed culture to produce transgenic plants; and selecting a transgenic plant comprising a shortened fragment of determined length and sequence that effects a desirable change in the fatty acid composition of seed oil. The present invention also includes a soybean seed that exhibits an oily composition having a strongly saturated fatty acid content and a moderately increased fatty acid content having a DNA sequence that suppresses expression.

Endogenous FAD2 in a plant cell, where the DNA sequence has a recombinant DNA sequence consisting of an FAD2 intron fragment. Another embodiment of the present invention is a nucleic acid molecule comprising a sequence of an FAD2-1 A intron, wherein the FAD2-1 A intron fragment is between about 60 to about 320 contiguous nucleotides. In an alternative embodiment, the present invention also includes a soybean seed having a first recombinant DNA sequence that suppresses the expression of endogenous soybean FAD2-1, which comprises an FAD2-1 intron of soybean, and a second recombinant DNA sequence expressing increased levels of a gene selected from the group consisting of KASI, delta-9 desaturase, KASIV, and combinations thereof. The present invention also includes a soybean plant cell that exhibits a fatty acid composition of seed oil comprising an oleic acid content of about 42% to about 85% by weight of the total fatty acids and an acid content. saturated fat of less than 8% by weight of the total fatty acids. Also included in the present invention is a soybean seed cell from a soybean that exhibits a fatty acid composition of seed oil comprising an oleic acid content of about 42% to about 85% by weight of the acids total fatty acids and a linolenic acid content of less than about 3% by weight of the total fatty acids.

The present invention also includes a nucleic acid molecule of an intron FAD2-1 A, wherein the intron FAD2-1 A is between about 60 to about 320 contiguous nucleotides. Also included is a recombinant DNA construct comprising an FAD2-1 intron fragment of soybean that is between 20 to about 420 contiguous nucleotides in length and a FATB gene fragment of soybean that is between about 40 and approximately 450 contiguous nucleotides in length. Another embodiment includes a recombinant nucleic acid molecule having a first DNA sequence that suppresses the endogenous expression of FAD2-1 and soybean FATB, where the first recombinant DNA sequence includes an FAD2-1 intron fragment that is found between about 20 and about 420 contiguous nucleotides in length, a FATB '3 UTR of soybean seed, and a FATB 5' UTR or CTP of soybean that encodes the area, and a second recombinant DNA sequence that increases the expression of at least one of the genes selected from the group that It consists of beta-ketoacyl-ACP synthase IV and delta-9 desaturase. The present invention also includes a non-mixed soybean oil having a fatty acid composition comprising an oleic acid content of about 42% to about 85% by weight of the total fatty acids and a saturated fatty acid content of about 1.5% to about 8% by weight of the total fatty acids; a non-mixed soybean oil that has a fatty acid composition comprising an oleic acid content of about 42% to about 85% by weight of the total fatty acids and a saturated fatty acid content of about 8% or less by weight of the total fatty acids; an unmixed soybean oil having a fatty acid composition comprising an oleic acid content of about 42% to about 85% by weight of the total fatty acids and a linolenic acid content of less than 3% by weight of total fatty acids; and an unmixed soybean oil having a fatty acid composition comprising an oleic acid content of about 42% to about 85% by weight of the total fatty acids, a saturated fatty acid content of about 8% or less in weight of the total fatty acids, and a linolenic acid content of about 1.5% or less by weight of the total fatty acids. The present invention also includes a soybean meal derived from a soybean seed exhibiting a fatty acid composition of seed oil comprising an oleic acid content of about 42% to about 85% by weight of the total fatty acids and a Saturated fatty acid content of 8% by weight of the total fatty acids. Also included is a soybean meal derived from a soybean seed exhibiting a fatty acid composition of seed oil comprising an oleic acid content of about 42% to about 85% by weight of the total fatty acids and a linolenic acid content of less than about 3% by weight of the total fatty acids. The present invention also includes a method for reducing the amount of FAD2 gene suppression obtained by expressing an RNAid construct comprising a heterologous FAD2 sequence consisting of a complete FAd2 intron or a complete FAD2 UTR, the method i) expressing a heterologous FAD2 sequence in a plant cell, wherein the heterologous FAD2 sequence is derived from an endogenous FAD2 gene in a plant cell and consists of an FAD2 intron fragment or a FAD2 UTR fragment; and ii) suppressing an endogenous FAD2 gene with the heterologous FAD2 sequence, wherein the amount of FAD2 gene deletion is less than the amount of gene expression obtained by the expression of a heterologous FAD2 sequence consisting of the full length of an FAD2 intron. or the full length of an FAD2 UTR. The present invention also includes a method for altering the oily composition of a plant cell by: transforming a plant cell with a heterologous FAD2 sequence derived from a part of an endogenous FAD2 gene, where the heterologous FAD2 sequence consists of an FAD2 intron fragment or a fragment of FAD2 UTR; and the growth of the plant cell under conditions where the transcription of the heterologous FAD2 sequence is initiated, whereby the oily composition is altered in relation to a plant cell with a similar genetic background but lacking the heterologous FAD2 sequence.

The present invention also includes a method for increasing the content of oleic acid and reducing the content of saturated fatty acid in a plant seed comprising: i) shortening the length of a first heterologous FAD2 sequence up to the amount of gene deletion FAD2 of a plant transformed with the first heterologous FAD2 sequence is at least partially reduced in relation to the amount of gene deletion FAD2 in a plant cell comprising a similar genetic background and a second heterologous FAD2 sequence, where the second heterologous FAD2 sequence consists of more sequences endogenous FAD2 than the first heterologous FAD2 sequence; ii) the expression of a heterologous FATB sequence capable of at least partially reducing the FATB gene expression in a plant cell in relation to the suppression of FATB in a plant cell with a similar genetic background but without the heterologous FATB sequence; iii) the growth of a plant comprising a genome with the first heterologous sequence FAD2 and the heterologous sequence FATB; and iv) cultivating a plant that produces seed with a reduced saturated fatty acid content relative to the seed of a plant that has a similar genetic background but lacks the first heterologous FAD2 sequence and the heterologous FATB sequence. The present invention also includes a method of modulating the fatty acid composition of oil from a seed of a temperate oil seed culture comprising isolating a fragment of a genetic element of at least 40 nucleotides in length that is capable of suppress the expression of an endogenous gene in the path of fatty acid synthesis; introducing the genetic element into a plant cell of a temperate oilseed crop; produce a transgenic plant; and selecting a transgenic plant seed comprising the genetic element that modulates the fatty acid composition of seed oil. In another embodiment, the present invention includes a soybean cell that exhibits a fatty acid composition of seed oil comprising an oleic acid content of about 42% to about 85% by weight of the total fatty acids and a content of of saturated fatty acid of less than 8% by weight of the total fatty acids. The present invention also includes a heterologous nucleic acid molecule comprising an FAD2-1 soybean intron fragment that is between about 20 and about 420 contiguous nucleotides in length and a fragment of the FATB soybean gene that is found between about 40 and about 450 contiguous nucleotides in length. In another embodiment, the present invention relates to a heterologous nucleic acid molecule comprising a nucleic acid sequence comprising an intron fragment of soybean FAD2-1 which is between about 20 and about 420 nucleotides in length, a FATB soybean gene fragment that is between about 40 and about 450 contiguous nucleotides in length, and a sequence of nucleic acid that increases the expression of one or both of beta-ketoacyl-ACP synthase IV and delta-9 desaturase. The present invention also relates to a method for decreasing the linolenic acid content of a soybean by i) introducing into a soybean cell a heterologous nucleic acid molecule comprising the nucleic acid sequence of at least two members of a FAD3 gene family; ii) expressing a nucleic acid sequence of an FAD3 gene capable of at least partially reducing endogenous FAD3 gene expression in a plant cell; iii) the development of a plant cell comprising a genome with the nucleic acid sequence of at least two members of the FAD3 gene family; and iv) cultivating the plant cell with reduced content of linolenic acid in relation to a plant cell having a similar genetic background but lacking at least two members of the FAD3 gene family. The present invention also includes a recombinant DNA construct with DNA fragments from at least two members of the FAD3 gene family. The present invention also includes a non-mixed soybean oil having a fatty acid composition comprising an oleic acid content of about 42% to about 85% by weight of the total fatty acids, a saturated fatty acid content of about 8% or less by weight of the total fatty acids, and a linolenic acid content of about 1.5% or less by weight of the total fatty acids.

BRIEF DESCRIPTION OF THE DRAWINGS Figures 1-4 each represent exemplary nucleic acid molecule configurations. Figures 5A-5D and 6A-6C each represent illustrative configurations of a first set of DNA sequences. Figures 7-20C each represent nucleic acid molecules of the present invention. Figure 21 represents the construct pMON68537. Figure 22 represents the construct pMON68539.

DETAILED DESCRIPTION OF THE INVENTION Description of Nucleic Acid Sequences SEQ ID NO: 1 is a nucleic acid sequence of an intron 1 FAD2-1 A. SEQ ID NO: 2 is a nucleic acid sequence of an intron 1 FAD2-1 B. SEQ ID NO: 3 is a nucleic acid sequence of a FAD2-1 B promoter. SEQ ID NO: 4 is a nucleic acid sequence of a genomic clone FAD2-1 A. SEQ ID NOs: 5 &; 6 are nucleic acid sequences of a FAD2-1 A 3 'UTR and 5'UTR, respectively. SEQ ID NOs: 7-13 are nucleic acid sequences of introns FAD3-1 A 1, 2, 3A, 4, 5, 3B, and 3C, respectively. SEQ ID NO: 14 is a nucleic acid sequence of an FAD3-1 C intron 4. SEQ ID NO: 15 is a nucleic acid sequence of a partial FAD3-1 A genomic clone. SEQ ID NOs: 16 & 17 are nucleic acid sequences of an FAD3-1 A 3 'UTR and 5'UTR, respectively. SEQ ID NO: 18 is a nucleic acid sequence of a partial FAD3-1 B genomic clone. SEQ ID NOs: 19-25 are nucleic acid sequences of introns FAD3-1 B 1, 2, 3A, 3B, 3C, 4 and 5, respectively. SEQ ID NOs: 26 & 27 are nucleic acid sequences of an FAD3-1 b 3 'UTR and 5'UTR, respectively. SEQ ID NO: 28 is a nucleic acid sequence of a genomic clone FATB-1. SEQ ID NOs: 29-35 are nucleic acid sequences of introns FATB-1 I, II, III, IV, V, VI and VII, respectively. SEQ ID NOs: 36 & 37 are nucleic acid sequences of a FATB1 - 3 'UTR and 5'UTR, respectively. SEQ ID NO: 38 is a nucleic acid sequence of a Cuphea pulcherrima KAS I gene.

SEQ ID NO: 39 is a nucleic acid sequence of a Cuphea pulcherrima KAS IV gene. SEQ ID NOs: 40 & 41 are nucleic acid sequences from Ricinus communis and Simmondsia Chinensis delta-9 desaturase genes, respectively. SEQ ID NO: 42 is a nucleic acid sequence of a cDNA FATB-2. SEQ ID NO: 43 is a nucleic acid sequence of a genomic clone FATB-2. SEQ ID NOs: 44-47 are nucleic acid sequences of FATB-2 I, II, III, IV, V, VI and VII, respectively. SEQ ID NOs: 48-60 are nucleic acid sequences of PCR primers. SEQ ID NOs: 61 & 62 are nucleic acid sequences of FAD3-1 C 3 'UTR and 5'UTR d of soybean, respectively.

Definitions "ACP" refers to a radical of acyl carrier protein. "Altered seed oil composition" refers to a seed oil composition of a transgenic or transformed plant of the invention having altered or modified levels of the fatty acids, in relation to a seed oil of a plant having a genetic background similar but that has not been transformed.

"Antisense suppression" refers to specific silencing of the gene that is induced by the introduction of an antisense RNA molecule. "Co-expression of more than one agent such as an mRNA or protein" refers to the simultaneous expression of one agent in the superposition of time frames in the same cell or tissue as another agent. "Coordinated expression of more than one agent" refers to the coexpression of more than one agent when the production of transcripts and proteins of said agents is carried out using a shared or identical promoter. "Complement" of a nucleic acid sequence refers to the complement of the sequence along its entire length. "Cosuppression" is the reduction in expression levels, usually at the RNA level, of a particular endogenous gene or gene family by expression of a homologous sense construct that is capable of transcribing mRNA of the same arrangement into chains such as the transcript of the endogenous gene. Napoli et al., Plant Cell 2: 279-289 (1990); van der Krol et al., Plant Cell 2291-299 (1990). "Crude soybean oil" refers to a soybean oil that has been extracted from soybeans, but has not been refined, processed, or mixed, although it can be degummed. "CTP" refers to a chloroplastic transit peptide, encoded by the "sequence encoding chloroplastic transit peptide." "When referring to proteins and nucleic acids here, "derivative" refers to directly (for example, by looking at the sequence of a known protein or nucleic acid and when preparing a protein or nucleic acid having a sequence similar, at least in part, to the sequence of the protein or acid known nucleic acid) or indirectly (for example, by obtaining a protein or nucleic acid from an organism which is related to a known protein or nucleic acid) obtaining a protein or nucleic acid from a known protein or nucleic acid. Other methods of "derivatization" of a protein or nucleic acid of a known protein or nucleic acid are known to a person skilled in the art. Double-stranded RNA (dsRNA "), double-stranded RNA interference (" RNAids ") and RNA interference (" RNAi ") refer to specific gene silencing that is induced by the introduction of a contract able to transcribe at least partially a double-stranded RNA molecule A "dsRNA molecule" and an "iRNA molecule" refer to a region of an RNA molecule that contains segments with nucleotide sequences in complementary and therefore can hybridize with each other and These double-stranded RNA molecules are capable, when introduced into a cell or organism, of at least partially reducing the level of mRNA species present in a cell or a cell of an organism. ARNds can be created after the live assembly of appropriate DNA fragments through illegitimate recombination and site-specific recombination as described in International Application No. PCT / US2005 / 00468 , registered on 1 1 of February 2005, which is hereby incorporated by reference in its entirety. "Exon" refers to the normal meaning of the term meaning a segment of nucleic acid molecules, usually DNA, that encodes part or all of an expressed protein. "Fatty acid" refers to free fatty acids and fatty acyl groups. "Gene" refers to a nucleic acid sequence that includes a 5 'promoter region associated with the expression of the gene product, any of the intron and exon regions, and 3' and 5 'untranslated regions associated with the expression of the gene product . "Genetic silencing" refers to the suppression of gene expression or down regulation of gene expression. A "family of genes" is two or more genes in an organism that encode proteins that exhibit similar functional attributes, and a "family member of genes" is any gene from the family of genes found within the genetic material of the plant, by example, a "FAD2 gene family member" is any FAD2 gene found within the genetic material of the plant. An example of two members of a gene family are FAD2-1 and FAD2-2. A family of genes can be further classified by the similarity of the nucleic acid sequences. A gene, FAD2, for example, includes alleles at the site. Preferably, a gene family member exhibits at least 60%, more preferably at least 70%, more preferably at least 80% nucleic acid sequence identity in the coding sequence portion of the gene. "Heterologist" means that they are not of natural origin as a whole. A nucleic acid molecule is to be "introduced" if it is inserted into a cell or organism as a result of human manipulation, regardless of whether it is indirect. Examples of introduced nucleic acid molecules include, but are not limited to, nucleic acids that have been introduced into cells via transformation, transfection, injection, and projection, and those that have been introduced into an organism via methods that include, but are not they limit, conjugation, endocytosis and phagocytosis. "Intron" refers to the normal meaning of the term as meaning a segment of nucleic acid molecules, usually DNA, that does not encode part or all of an expressed protein, and that, under endogenous conditions, is transcribed into RNA molecules, but that is spliced out of the endogenous RNA before the RNA is translated into a protein. An "intron dsRNA molecule" and an "intron RNAi molecule" refer to a double-stranded RNA molecule capable, when introduced into a cell or organism, of at least partially reducing the level of a few mRNA species present in a cell or a cell of an organism where the double-stranded RNA molecule exhibits sufficient identity to an intron of a gene present in the cell or organism to reduce the level of an mRNA containing this intron sequence. An "unsaturated" oily composition contains between 3.6 and 8 percent of saturated fatty acids. A "medium oleic soybean seed" is a seed having between 50% and 85% oleic acid present in the oily composition of the seed. An "low linolenic content" oily composition containing less than about 3% linolenic acid by weight of the total fatty acids. The term "non-coding" refers to the sequences of nucleic acid molecules that do not encode part or all of an expressed protein. Non-coding sequences include but are not limited to neutrons, promoter regions, 3 'untranslated regions (3'UTR), and 5' untranslated regions (5'UTR). The term "oily composition" refers to levels of fatty acids. A promoter that is "operably linked" to one or more nucleic acid sequences is capable of driving expression of one or more nucleic acid sequences, including multiple coding or non-coding nucleic acid sequences arranged in a polycistronic configuration. "Physically linked" nucleic acid sequences are nucleic acid sequences that are found in a single nucleic acid molecule. A "plant" includes the reference to whole plants, organs of plants (for example leaves, stems, roots etc), seeds, and plant cells and progeny thereof. The term "plant cell" includes, without limitation, seed suspension cultures, embryos, meristematic regions, callus tissue, leaves, roots, shoots, gametophytes, sporophytes, pollen, and microspores. "Plant promoters", include, without limitation, plant viral promoters, promoters derived from plants, and synthetic promoters capable of functioning in a plant cell to promote the expression of an mRNA. A "polycistronic gene" or "polycistronic mRNA" is any gene or MRNA containing transcribed nucleic acid sequences that correspond to nucleic acid sequences of more than one gene that is targeted for suppression or expression. It is understood that said polycistronic genes or mRNA may contain sequences corresponding to introns, 5'UTR, 3'UTR, transit peptide coding sequences, exons, or combinations thereof, and that a recombinant polycistronic gene or mRNA may, for example, without limitation, contain sequences corresponding to one or more UTR of a gene and one or more introns of a second gene. A "seed-specific promoter" refers to a promoter that is preferably or exclusively active in a seed. "Preferred activity" refers to promoter activity that is substantially greater in the seed than in other tissues, organs or organelles of the plant.

"Seed-specific" includes without limitation activity in the aleurone layer, endosperm, and / or embryo of the seed. "Sense intron suppression" refers to the silencing gene that is induced by the introduction of a sense intron or its fragment. The sense intron suppression is described, for example by Fillatti in PCT WO 01/14538 A2. "Simultaneous expression" of more than one agent such as an mRNA or protein refers to the expression of one agent at the same time as another agent. Said expression can only partially overlap and can also occur in different tissue or at different levels. "Total oil level" refers to the total aggregate amount of fatty acid without considering the type of fatty acid. As used herein, the total oil level does not include the glycerol backbone. "Transgene" refers to a nucleic acid sequence associated with the expression of a gene introduced into an organism. A transgene includes, but is not limited to, an endogenous gene or a gene not of natural origin in the organism. A "transgenic plant" is any plant that stably incorporates a transgene in a manner that facilitates the transmission of this transgene from a plant by any sexual or asexual method. An oily composition "zero saturated" contains less than 3. 6 percent saturated fatty acids. When referring to proteins and nucleic acids here, the use of ordinary capital letters, for example, "FAD2", indicates a reference to an enzyme, protein, polypeptide, or peptide, and the use of cursive capital letters, for example, "FAD2" is used to refer to nucleic acids, including without limitation genes, cDNA, and mRNA. A cell or organism can have a family of more than one gene encoding a particular enzyme, and the capital letter that follows the terminology of the gene (A, B, C) is used to designate the family member, ie, FAD- 2-1A is a different family member of the FAD2-1B gene. As used herein, any exposed range is inclusive of the end points of the interval unless stated otherwise.

A. Agents The agents of the invention will preferably be "biologically active" with respect to a structural attribute, such as the ability of a nucleic acid molecule to hybridize to another nucleic acid molecule, or the ability of a protein to be linked by an antibody (or to compete with another molecule for said binding). Alternatively, said attribute can be catalytic and thus involves the agent's ability to mediate a chemical reaction or response. The agents will preferably be "substantially purified". The term "substantially purified" as used herein, refers to a molecule separated from substantially all other molecules normally associated with it in its native environmental conditions. More preferably a substantially purified molecule is the predominant species present in a preparation. A purified molecule can be substantially greater than 60% free, greater than 75% free, preferably greater than 90% free, and more preferably greater than 95% free of the other molecules (exclusive of solvent) present in the natural mixture. The term "substantially purified" is not intended to include molecules present in their native environmental conditions. The agents of the invention can also be recombinants. As used herein, the term "recombinant" means any agent (eg, includes but is not limited to DNA or peptide), that is, or results, however, indirectly, from human manipulation of a nucleic acid molecule. It is also understood that the agents of the invention can be labeled with reagents that facilitate the targeting of the agent, for example, fluorescent labels, chemical labels, and / or modified bases. Agents of the invention include DNA molecules having a nucleotide sequence that is capable of being transcribed in sense and anti-sense orientations that form at least one RNA molecule that is, at least in part, double stranded. In a preferred embodiment, an agent of the invention is a double-stranded RNA molecule having a nucleotide sequence that is a fragment of FAD2, FATB or FAD2 and FATB. In another embodiment, an agent of the present invention is a DNA molecule capable of being transcribed to produce sense and antisense orientations of a nucleotide sequence in a host cell. In another embodiment, a nucleic acid molecule can have a sequence of nucleotide in a sense orientation and in an antisense orientation, or in another embodiment, a nucleic acid molecule can have a nucleotide sequence in a sense orientation or an antisense orientation. Said nucleotide sequences can be operably linked to the same promoter, different promoters, a single promoter, or more than one promoter. Said nucleotide sequences may be in a single DNA molecule or more than one DNA molecule. Agents of the invention include nucleic acid molecules comprising a DNA sequence that is at least 50%, 60% or 70% identical over its entire length to a coding or non-coding region of the plant, or to an acid sequence nucleic that is complementary to a coding or non-coding region of the plant. More preferably they are DNA sequences which are, in their entire length, at least 80% identical, at least 85% identical, at least 90% identical; at least 95% identical, at least 97% identical; at least 98% identical, at least 99% identical or 100% identical to a coding region or non-coding region of the plant, or to a nucleic acid sequence that is complementary to a coding or non-coding region of the plant . "Identity" as is well understood in the art, is a relationship between two or more polypeptide sequences or two more nucleic acid molecule sequences, as determined by the comparison of the sequences. In the art, "identity" also means the degree of sequence relationship between polypeptide or nucleic acid molecule sequences, as determined by the balance between chains of said sequences. "Identity" can be easily calculated by known methods including, but not limited to, those described in Computational Molecular Biology, Lesk, ed., Oxford University Press, New York 1988; Biocomputing: Informatics and Genome Projects, Smith, ed., Academic Press, New York 1993; Computer Analysis of Sequence Data, Parí I, Griffin and Griffin, eds., Humana Press, New Jersey 1994; Sequence Analysis in Molecular Biology, von Heinje, Academic Press 1987; Sequence Analysis Primer, Gribskov and Devereux, eds., Stockton Press, New York 1991; and Carillo and Lipman, SIAM J. Applied Math, 48: 1073 1988. Methods to determine identity are designed to provide the greatest balance between the sequences tested. further, methods to determine identity are encoded in publicly available programs. Computer programs which can be used to determine identity between two sequences include, but are not limited to, GCG, a series of five BLAST programs, three designed for nucleotide sequence issues (BLASTN, BLASTX, and TBLASTX) and two designed for protein sequence issues (BLASTP and TBLASTN). The BLASTX program is publicly available from NCBI and other sources, for example, BLAST Manual, AltschuI et al., NCBI NLM NIH, Bethesda, MD 20894; AltschuI et al., J. Mol. Biol. 215: 403-410 (1990). The well-known Smith Waterman algorithm can also be used to determine identity. Parameters for comparison of polypeptide sequence they usually include the following: Algorithm: Needleman and Wunsch, J. Mol. Biol. 48: 443-453 (1970); Comparison matrix: BLOSSUM62 by Hentikofí and Hentikofí, Proc. Nati Acad. Sci. USA 89: 10915-10919 (1992); Separation Penalty 12: Gap Length Penalty: 4. A program that can be used with these parameters is publicly available as the "separation" program from Genetics Computer Group ("GCG"), Madison, Wisconsin. The above parameters together with no penalty for the final separation are omission parameters for peptide comparisons. Parameters for nucleic acid molecule sequence comparison include the following: algorithm: Needleman and Wunsch, J. Mol. Bio. 48: 443-453 (1970) Comparison matrix: equilibrium - + 10; imbalance = 0; Separation penalty: 50; Separation length penalty: 3. As used herein, "% identity" is determined using the above parameters as the default parameters for nucleic acid molecule sequence comparisons and GCG "separation" program, version 10.2 . Subsets of the nucleic acid sequences of the present invention include fent nucleic acid molecules. "Nucleic acid molecule fent" refers to a piece of a larger nucleic acid molecule, and may consist of a portion or portions significant of, or indeed greater than, the larger nucleic acid molecule. The nucleic acid molecule fent may comprise a smaller oligonucleotide having from about 15 to about 400 contiguous nucleotides and more preferably, about 15 to about 45 contiguous nucleotides, about 20 to about 45 contiguous nucleotides, about 15 to about 30 contiguous nucleotides, about 21 to about 30 contiguous nucleotides, about 21 to about 25 contiguous nucleotides, about 21 to approximately 24 contiguous nucleotides, approximately 19 to approximately 25 contiguous nucleotides, or approximately 21 contiguous nucleotides. The fent of nucleic acid molecules may consist of a significant portion or portions of, or indeed greater than, a coding or non-coding region of the plant, or alternatively may comprise smaller oligonucleotides. In a preferred embodiment, a fent shows 100% identity for the coding or non-coding region of the plant. In another preferred embodiment, a fent comprises a portion of a larger nucleic acid sequence. In another aspect, a fent of nucleic acid molecule has a nucleic acid sequence having at least 15, 25, 50, 100, 200, 300, or 400 contiguous nucleotides of a nucleic acid molecule of the present invention. In a preferred embodiment, a nucleic acid molecule has a nucleic acid sequence having at least 15, 25, 50, 100, 200, 300 or 400 contiguous nucleotides of a coding or non-coding region of the plant. In a more preferred embodiment, a nucleic acid molecule has a nucleic acid sequence that has approximately 1, 2, 5, 10, 20, 30, 40, 50, 60, 70, 80 or 90% of the contiguous nucleotides of an entire coding or non-coding region. In a preferred embodiment, a whole coding or non-coding region can be an element of the gene selected from a whole gene, a single exon, or a single intron, a signal sequence, or a non-translated region (UTR). An element of the gene that does not have the entire sequence of an entire genetic element can be a fent of a gene element. In a genetic aspect of the present invention, a genetic element is at least 40 nucleotides in length. In one aspect of the present invention, a fent of a gene is a portion of the entire gene element and said fent contains contiguous nucleotides of about 1, 2, 5, 10, 20, 30, 40, 50, 60, 70, 80 or 90% of the entire gene element. In one aspect of the present invention, a nucleic acid molecule fragment is between about 5% -about 80%, between about 10% -about 70%, between about 10% -about 60%, between about 10% -about 50 %, between about 25% - about 60%, between about 25% - about 50%, between about 40% - about 60%, between about 40% - about 80%, between about 50% - about 90% length of a element of the entire gene. In a preferred embodiment, an FAD2-1 intron fragment is between about 20 and about 420, about 30 and about 420, between about 40 and about 320, between about 50 and about 200, between about 50 and about 400, between about 50 and about 420, between about 60 and about 320, about 70 and about 220, between about 100 and about 200 , between about 100 and about 320, between about 150 and about 200, between about 150 and about 220, between about 150 and about 400, between about 200 and about 300, or between about 300 and about 400 contiguous nucleotides. In another preferred embodiment, an FAD2-1 intron fragment is about 100, about 150, about 200, about 220, about 250, about 300, about 320, or about 350 contiguous nucleotides in length. In another preferred embodiment, an intron FAD2-1 fragment is reduced in length by about 20, about 40, about 60, about 80, about 100, about 120, about 140, about 160, about 180, about 200, about 220, about 240, about 260, about 280, about 290, about 300, about 320, about 340, about 360, about 380, about 400 contiguous nucleotides with the length of SEQ ID NO: 1. For all these fragments of intron FAD2-1, truncation or deletion can start at the 5 'end, start at the 3' end, or can be internal to an FAD2-1 intron. For all these intron fragments FAD2-1, the sequence of an intron FAD2-1 can be SEQ ID NO: 1. In a preferred embodiment, a fragment of a FA TB gene is about 80 to about 450, about 100 to about 500, about 70 to about 500, about 200 to about 400, about 150 to about 300, about 250 to about 350, about 200 to about 350 contiguous nucleotides of a FATB gene. In a preferred embodiment, a FATB fragment is derived from a half of the total nucleotides in FATB starting at the 5 'end. For all these fragments of FATB, truncation or deletion can start at the 5 'end, starting at the 3' end, or be internal to FATB. In a preferred embodiment, a fragment of FATB is derived from a half of the total nucleotides in FATB that starts at the 5 'end of FATB, is derived from a third of the total nucleotides in FATB that close at the 5' end. In a particularly preferred embodiment, a FATB fragment contains a transit peptide coding sequence, which preferably codes for the chloroplast transit peptide. In a particularly preferred embodiment, a fragment of FATB is a fragment of a transit peptide coding sequence, which preferably codes for the chloroplast transit peptide. In another modality in particular preferred, a FATB fragment further includes about 20, about 25, about 30, about 35, 38, 39, 40, 41, 42, 43, about 45, about 50, about 55, or about 60 contiguous nucleotides of a FATB 5 'UTR. In a more preferred embodiment, a fragment includes a combination of two or more discontinuous fragments or separated gene elements, such as FATB 3'UTR fused to a FATB 5'URT. Agents of the invention include nucleic acid molecules. For example, without limitation, in one aspect of the present invention, the nucleic acid molecule of the present invention comprises an intron sequence of SEQ ID NOs: 19, 20, 21, 22, 23, 25, 32, 33, 34 , 35, 44, 45, 46 or 47 or their fragments or their complements. In another aspect of the invention, the nucleic acid molecule comprises a nucleic acid sequence, which, when introduced into a cell or organism, is capable of suppressing the production of RNA or protein while simultaneously expressing, co-expressing or co-expressing another RNA or protein. In one aspect of the invention, the nucleic acid molecule comprises a nucleic acid sequence, which when introduced into a cell or organism is capable of suppressing, at least partially reducing, reducing, substantially reducing, or effectively eliminating RNA expression. FAD2, FAD3, and / or endogenous FATB although at the same time co-expressing, simultaneously expressing, or coordinatingly expressing at least one of a beta-ketoacyl-ACP synthase I, beta-ketoacyl-ACP synthase IV, delta-9 desaturase, and / or CP4 EPSPS RNA or protein.

By suppressing, at least partially reducing, reducing, substantially reducing, or effectively eliminating the expression of at least one or more endogenous genes, the amount of FAD2 and / or FAD3 available in a plant cell is decreased, i.e. state levels Stationary protein are reduced, and a decreased percentage of polyunsaturated fatty acids such as linoleate (C18: 2) and linolenate (C18: 3) can be provided. Modifications in the fatty acid pool available for incorporation into triacylglycerols can similarly affect the composition of oils in the plant cell. In this way, a decrease in the expression of FAD2 and / or FAD3 can result in an increased proportion of monounsaturated fatty acids such as oleate (C18.1). When the amount of FATB is decreased in a plant cell, a decreased amount of saturated fatty acids such as palmitate and stearate can be provided. In this way, a decrease in the expression of FATB can result in an increased proportion of unsaturated fatty acids such as oleate (18: 1). The simultaneous suppression of FAD2, FAD3 and FATB expression results in directing the FAS path towards a complete increase in monounsaturated fatty acids of 18 carbons length, such as oleate (C18: 1). See U.S. Pat. No. 5,955,650. By increasing the amount of beta-ketoacyl-ACP synthase I (KAS I) and / or beta-ketoacyl-ACP IV synthase IV (KAS IV) available from a plant cell, a decreased percentage of 16: 0-ACP can be provided, driving to a increased percentage of 18: 0-ACP. A greater amount of 18: 0-ACP in combination with the simultaneous suppression of one or more of FAD2, FAD3, and FATB, thereby helping to direct the oily composition towards a total increase in oleate (C18: 1). By increasing the amount of delta-9 desaturase available in a plant cell, an increased percentage of unsaturated fatty acids can be provided, resulting in a total decrease in stearate and total saturates. These combinations of increased and decreased enzyme expression can be manipulated to produce oily compositions, including fatty acids, which have an increased oleate level, decreased linoleate, linolenate, stearate, and / or palmitate levels, and a decreased total level of saturated . The increase of gene expression in plants can occur through the introduction of extra copies of gene coding sequences in the plant cell or, preferably, the incorporation of extra copies of gene coding sequences in the plant genome. Overexpression can also occur through increasing the activities of regulatory mechanisms that regulate gene expression, that is, over-regulation of gene expression. The production of CP4 EPSPS in a plant cell provides the plant cell with resistance or tolerance to glyphosate, which provides a convenient method for the successful identification of transformants via glyphosate-tolerant selection. The suppression of gene expression in plants, too known as gene silencing, it occurs at the transcriptional level and post-transcriptional level. There are several methods for the suppression of expression of endogenous sequences in a host cell, including, but not limited to, anti-sense deletion, co-suppression, ribozymes, sense and antisense combinations (double-stranded RNAi), promoter silencing, and DNA binding proteins such as "zinc finger" zinc finger proteins (see, for example, WO 98/53083, WO 01/14538, and US Patent No. 5,759,829 (Schewmaker)). Certain of these mechanisms are associated with nucleic acid homology at the DNA or RNA level. Said homology refers to the similarity in DNA or protein sequences within the same species or between different species. Gene silencing occurs if the DNA sequence introduced into a host cell is sufficiently homologous to an endogenous gene that transcription of the introduced DNA sequence will induce silencing of the transcriptional or post transcriptional gene of the endogenous gene. Sufficient homology for the suppression of steady state expression levels can be at least 50%approximately 60%, or approximately 70% identical over the entire length of a DNA sequence to a coding region or non-coding region of the plant, or to a nucleic acid sequence that is complementary to a coding or coding region. no coding of the plant. More preferably they are DNA sequences which are, over their entire length, at least 80% identical, at least 85% identical, at least 90% identical, at least 95% identical; at least 97% identical; at least 98% identical; at least 99% identical or 100% identical to a coding region or non-coding region of the plant, or to a nucleic acid sequence that is complementary to a coding or non-coding region of the plant. In plants, double-stranded RNA molecules can induce specific sequence silencing. Gene silencing is often referred to as double-stranded RNA ("RNAids") in plants, an RNA or RNAi interference in Caenorhabditis elegants and in animals, and as chelation in fungi. In a preferred embodiment, the nucleic acid molecule of the present invention comprises a first set of DNA sequences, each of which exhibits sufficient homology to one or more coding or non-coding sequences of a plant gene such that when expresses, is capable of effectively removing, substantially reducing, or at least partially reducing the level of a mRNA or protein transcript encoded by the gene from which the coding or non-coding sequence is derived, or any gene having homology to the sequence of coding or non-coding of the objective. In a preferred embodiment, the nucleic acid molecule of the present invention comprises (a) a first set of DNA sequences, each of which exhibits sufficient homology to one or more coding or non-coding sequences of a plant gene such that when expressed, it is capable of effectively eliminating, substantially reducing, or at least partially reducing the level of a mRNA or protein transcript encoded by the gene from which the coding or non-coding sequence is derived, or any gene which has homology to the non-coding sequence of the target, and (b) a second set of DNA sequences, each of which exhibits sufficient homology to a plant gene so that when expressed, it is capable of at least partially increasing, increasing, increasing, or substantially increasing the level of a mRNA or protein transcript encoded by the gene. As used herein, "a set" of DNA sequences may be one or more sequences, which encode or do not code for a protein. For example, a first set of DNA sequences can be composed of only one promoter, one non-coding region, and one terminator. A second set of DNA sequences may or may not be present after or before the first set of DNA sequences. As used herein, "a reduction" in the level or amount of an agent such as a protein or mRNA means that the level or amounts are reduced relative to a cell or organism lacking an ADBN sequence capable of reducing the agent. For example, "at least a partial reduction" refers to a reduction of at least 25%, "a substantial reduction" refers to a reduction of at least 75%, and "an effective elimination" refers to a reduction of more than of 95%, all reductions which in the level or amount of the agent are relative to a cell or organism lacking a DNA sequence capable of reducing the agent. As used herein, a level or amount "increased" or "increased" of an agent such as a protein or mRNA means that the level or amount is higher than the level or amount of the agent present in a cell, tissue or plant with a similar genetic background but lacking a nucleic acid molecule introduced that encodes the protein or mRNA. For example, a "at least partially increased" level refers to an increase of at least 25%, and a "substantially increased" level refers to an increase of at least 100%, all increases in the level or amount of a agent are relative to the level or amount of agent that is present in a cell, tissue or plant with a similar genetic background but lacks an introduced nucleic acid molecule encoding the protein or mRNA. In a preferred embodiment, an increase in expression can be any expression where the protein is heterologous to the system. For example, any expression of CP4 EPSPS can be an increase in expression if there is no expression in the plant before for the introduction of a nucleic acid molecule encoding the protein. When the levels of an agent are compared, such a comparison is preferably carried out between organisms with a similar genetic background. Preferably, a similar genetic background is a background where the organisms are shared compared to 50% or more, more preferably 75% or more, and, even more preferably 90% or more of sequence identity of the nuclear genetic material. In another preferred aspect, a similar genetic background is a background where the organisms that are compared are plants, and the plants are isogenic except for any genetic material originally introduced using plant transformation techniques. Measurements of the level or amount of an agent can be carried out by any suitable method, non-limiting examples of which include comparison of mRNA transcript levels, protein or peptide levels, and / or phenotype, especially oil content. As used herein, mRNA transcripts include processed or unprocessed mRNA transcripts, and proteins or peptides include proteins or peptides with or without any post-translational modification. The DNA sequences of the first set of DNA sequences can be coding sequences, intron sequences, 3'UTR sequences, 5'UTR sequences, promoter sequences, other non-coding sequences, or any combination of the above. The first set of DNA sequences encoding one or more sequences which, when expressed, are capable of selectively reducing either or both of the protein and the transcript encoded by a gene selected from the group consisting of FAD2, FAD3 and FATB. In a preferred embodiment, the first set of DNA sequences is capable of expressing the antisense RNA, in which the individual antisense sequences can be linked in a transcript, or they can be in unbound individual transcripts. In a further preferred embodiment, the first set of DNA sequences are physically linked sequences that are capable of expressing a single dsRNA molecule. In a different preferred embodiment, the first set of DNA sequences is capable of expressing sense co-deletion RNA, in the which individual sense sequences can be linked in a transcript, or they can be in unbound individual transcripts. Exemplary embodiments of the first set of DNA sequences are described in part B of the detailed description, and in the examples. The second set of DNA sequences encodes one or more sequences of which, when expressed, are capable of increasing either or both of the protein and transcript encoded by a gene selected from the group consisting of synthase I beta-ketoacyl-ACP ( KAS I), synthase IV beta-ketoacyl-ACP (KAS IV), delta-9 desaturase, and CP4 EPSPS. The DNA sequences of the second set of DNA sequences can be physically linked sequences. Exemplary embodiments of the second set of DNA sequences are described below in Parts C and D of the detailed description. In this manner, the present invention provides methods for altering the composition of fatty acids and compounds containing said fatty acids, such as oils, waxes and fats. The present invention also provides methods for the production of particular fatty acids in host cell plants. Said methods employ the use of expression cassettes described herein for the modification of the FAS path of host plant cell.

B. First set of DNA sequences In one aspect of the present invention, an acid molecule nucleic comprises a first set of DNA sequences, which when introduced into a cell or organism, expresses one or more sequences capable of effectively removing, substantially reducing, or at least partially reducing the levels of mRNA or protein transcripts encoded by one or more genes. Preferred aspects include as an objective an endogenous gene, a plant gene, and a non-viral gene. In one aspect of the present invention, a gene is a FAD2, FAD3 or FATB gene. In one aspect, a nucleic acid molecule of the present invention comprises a DNA sequence that exhibits sufficient homology to one or more coding or non-coding sequences of a plant gene, that when introduced into a plant cell or plant and it is capable of effectively eliminating, substantially reducing, or at least partially reducing the level of a mRNA transcript or protein encoded by the gene from which the coding or non-coding sequence or sequences are derived. The DNA sequences of the first set of DNA sequences transcribe RNA sequences or RNA fragments that exhibit at least 90%, preferably at least 95%, more preferably at least 98%, or more preferably 100% identity for a coding or non-coding region derived from the gene which is to be deleted. Said percent identity can be compared to another nucleic acid fragment. Preferably, the non-coding sequence is about 3'UTR, 5'UTR, a fraction of a protein coding sequence or a intron of a plant gene. More preferably, the non-coding sequence is a 3'UTR, 5'UTR promoter sequence, or an intron of a plant gene. The intron can be located between exons, or located within about 5 'or 3'UTR of a plant gene. The coding sequence is preferably a fraction of a coding frame of the protein. The sequence or sequences of the first set of DNA sequences can be designated to produce dsRNA, a sense suppression RNA, or an antisense RNA or any other deletion transcript in order to achieve the desired effect when introduced into a plant cell or plant . Said DNA sequence or sequences can be a fragment of nucleic acid molecules. A plant intron can be any plant intron of a gene, whether endogenous or introduced. The nucleic acid sequences of said intron organisms can be obtained or derived from a multitude of sources, including, without limitation, databases such as EMBL and Genbank which can be found on the internet at ebi.ac.uk/swisprot/; expasy.ch/; embl-heidelberg.de/; and ncbi.nlm.nih.gov. The nucleic acid sequences of said introns can also be derived, without limitation, from sources such as the GENSCAN program which can be found on the internet at genes.mit.edu/GENSCAN.html. Additional introns can also be obtained by methods which include, without limitation, classification of a genomic library with a probe from known exon or intron sequences, which compare the genomic sequence with its corresponding cDNA sequence, or cloning an intron such as soybean cDNA by aligning a genomic sequence of another organism, such as, for example, Arabidopsis. In addition, other intron nucleic acid sequences will be apparent to one of ordinary skill in the art. The methods described above can also be used to derive and obtain other non-coding sequences, including but not limited to, promoter sequences, 3'UTR sequences, and 5'UTR sequences. A "FAD2" desaturase "12" or "omega-6 desaturase" gene encodes an enzyme (FAD2) capable of catalyzing the insertion of a double bond in a fatty acyl radical in the twenty position counted from the carboxyl terminus. FAD2-1"is used to refer to the FAD2 gene that is naturally expressed in a specific manner in the seed tissue, and the term" FAD2-2 'is used to refer to an FAD2 gene that is (a) a gene different from a FAD2-1 gene and (b) is naturally expressed in multiple tissues, including the seed. Representative FAD2 sequences include, without limitation, those set forth in the U.S. Patent Application. No. 10 / 176,149 registered on June 21, 2002, and in SEQ ID NOs: 1-6. A "FAD3", "desaturase? 15" or "omega-3 desaturase" gene encodes an enzyme (FAD3) capable of catalyzing the insertion of a double bond into a fatty acyl radical at position fifteen counted from the carboxyl terminus. "FAD3-1, FAD3-A, FAD3-B and FAD3-C" are used to refer to the members of the FAD3 gene family that are expressed naturally in multiple tissues, including the seed. Representative FAD3 sequences include, without limitation, those set forth in the U.S. Patent Application. No. 10 / 176,149 registered on June 21, 2002 and in SEQ ID NOs: 7-27. A "FATB 'or" palmitoyl-ACP thioesterase "gene encodes an enzyme (FATB) capable of catalyzing the hydrolytic cleavage of carbon-sulfur thioester in the pantothene prosthetic group of palmitoyl-ACP as its preferred reaction. ACP thioesters of fatty acid may also be catalyzed by this enzyme Representative FATB-1 sequences include, without limitation, those set forth in U.S. Provisional Application No. 60 / 390,185 filed June 21, 2002; US Patent Nos. 5,955,329; 5,723,761; 5,955,650 and 6,331,664; and SEQ ID NOs: 28-37. Representative FATB sequences include, without limitation, those set forth in SEQ ID NOs: 42-47.

C. Second set of DNA sequences In one aspect of the present invention, a nucleic acid molecule comprises a second set of DNA sequences, which when introduced into a cell or organism, is capable of partially increasing, increasing, increasing, or to substantially increase the levels of mRNA transcripts or proteins encoded by one or more genes. In one aspect of the present invention, a gene is an endogenous gene. In another aspect of the present invention, a gene can be a heterologous gene. In a Preferred aspect, heterologous and endogenous genes can be in the same nucleic acid molecule. In one aspect of the present invention, a gene is a plant gene. In another aspect of the present invention, a gene is a truncated gene where the truncated gene is capable of catalyzing the reaction catalyzed by the full gene. In one aspect of the present invention, a gene is a beta-ketoacyl-ACP synthase I gene, beta-ketoacyl-ACP synthase IV gene, delta 9 desaturase gene, CP4 EPSPS gene or a combination of these genes. A gene of the present invention can be any gene, either endogenous or introduced. Nucleic acid sequences of said genes can be derived from a multitude of sources, including, without limitation, databases such as EMBL and Genbank which can be found on the internet at ebi.ac.uk/swisprot/; expasy.ch/; embl-heidelberg.de/; and ncbi.nlm.nih.gov. The nucleic acid sequences of said genes can also be derived, without limitation, from sources such as the GENSCAN program which can be found on the internet at genes.mit.edu/GENSCAN.html. Additional genes can also be obtained by methods which include, without limitation, the classification of a genomic library or a cDNA library with a probe of both known gene sequences, cloning of a gene by aligning a gene or probe from another organism , such as, for example, Arabidopsis. In addition, other gene nucleic acid sequences will be apparent to a person with ordinary skill in the art. Additional genes may, for example without limitation, be amplified by the polymerase chain reaction (PCR) and used in one embodiment of the present invention. In addition, other gene nucleic acid sequences will be apparent to a person with ordinary skill in the art. Automatic nucleic acid synthesizers can be used for this purpose, and to make a nucleic acid molecule having a sequence also found in a cell or organism. In place of such synthesis, nucleic acid molecules can be used to define a pair of primers that can be used with the PCR to amplify and obtain any desired nucleic acid molecule or fragment of a first gene. A "KAS I" gene or beta-ketoacyl-ACP synthase I encodes an enzyme (KAS I) capable of catalyzing the elongation of a fatty acyl radical to a palmitoyl-ACP (C16: 0) .Representative KAS I sequences include, without limitation, those set forth in U.S. Patent No. 5,475,099 and PCT publication WO 94/10189, and in SEQ ID NO: 38. A "KAS IV" or "beta-ketoacyl-ACP synthase IV" gene encodes an enzyme (KAS) IV) able to catalyze the condensation of medium chain acyl-ACP and increase the production of 18: 0-ACP.Representative KAS IV sequences include, without limitation, those set forth in PCT publication WO 98/46776, and in SEQ ID. NO: 39. A gene "delta-9 desaturase" or "stearoyl-ACP desaturase" or "Omega-9 desaturase" encodes an enzyme capable of catalyzing the insertion of a double bond into a fatty acyl radical at the nine position counted from the carboxyl terminus. A preferred delta-9 desaturase of the present invention is a delta-9 cyanobacterial plant or desaturase, and more preferably a delta-9 desaturase which is found in an organism selected from the group consisting of Cartharmus tinctorius, Ricinus communis, Simmonsia chinensis, and Brassica campestris. Representative delta-9 desaturase sequences include, without limitation, those set forth in the U.S. Patent. No. 5,723,595 and SEQ ID NOs: 40-41. A "CP4 EPSPS" or "CP4 5-enolpyruvylshikimate-3-phosphate synthase" gene encodes an enzyme (CP4 EPSPS) capable of conferring a substantial degree of glyphosate resistance in the plant cell and plants generated thereof. The CP4 EPSPS sequence can be a CP4 EPSPS sequence derived from Agrobacterium tumefaciens sp. CP4 or a variant or its synthetic form, as described in the patent of E.U.A. No. 5,633,435. Representative CP4 EPSPS sequences include, without limitation, those set forth in U.S. Patents. Nos. 5,627,061 and 5,633,435.

D. Recombinant Vectors and Constructs One or more of the nucleic acid constructs of the invention can be used in plant transformation or transfection. Levels of products such as transcripts or proteins can be increased or decreased through an organism such as a plant or located in one or more specific organs or tissues of the organism. For example, product levels may be increased or decreased in one or more tissues and organs of a plant including, without limitation: roots, tubercle, stems, leaves, trunks, fruit, berries, nuts, bark, pod, seeds and flowers. A preferred organ is a seed. For example, exogenous genetic material can be transferred into a plant cell and the plant cell regenerated into a whole, fertile or sterile plant or part of the plant. "Exogenous genetic material" is any genetic material, whether of natural origin or otherwise, from any source that is capable of being inserted into any organism. Said exogenous genetic material includes, without limitation, nucleic acid molecules and constructs of the present invention. Exogenous genetic material can be transferred into a host cell by the use of a DNA vector or construct designed for that purpose. Similarly, a virus can transfer exogenous genetic material into a host cell. Exogenous genetic material may have a DNA sequence identical to the endogenous gene, but has been re-introduced to the host cell by the use of a DNA vector or construct for the purpose of endogenous gene deletion expression. The design of said vector is generally within a person skilled in the art (See, for example, Plant Molecular Biology: A Laboratory Manual, Clark (ed.), Springer, New York (1997)). In a preferred embodiment, exogenous genetic material is recombinant DNA. A construct or vector may include a functional promoter in a plant cell, or a plant promoter, to express a nucleic acid molecule of choice. A number of promoters that are activated in plant cells have been described in the literature, and the CaMV 35S promoters and FMV are preferred for use in plants. Other examples of preferred promoters include bean arcelin and 7S alpha. Additional preferred promoters are increased or duplicate versions of CaMV 35S and FMV 35S promoters. Odell et al., Nature 313: 810-812 (1985); Patent of E.U.A. No. 5,378,619. Additional promoters that can be used are described, for example, in US Patents. 5,378,619; 5,391, 725; 5,428,147; 5,447,858; 5,608,144; 5,608,144; 5,614,399; 5,633,441; 5,633,435; and 4,633,436. In addition, a specific tissue enhancer can be used. Particularly preferred promoters can also be used to express a nucleic acid molecule of the present invention in seeds or fruits. Indeed, in a preferred embodiment, the promoter used is a seed-specific promoter. Examples of such promoters include the 5 'regulatory regions of such genes as napin (Kridl et al., Seed Sci. Res. 1: 209-219 (1991)), phaseolin, desaturase stearoyl-ACP, 7Sa, 7Sa' (Chen et al. al., Proc. Nati, Acad. Sci., 83: 8560-8564 (1986)), USP, arcelin and oleosin. Preferred promoters for expression in the seed are 7Sa, 7Sa ', napin and FAD2-1 A promoters. Constructs and vectors can also include other genetic elements, including but not limited to, 3' transcriptional terminators, 3 'polyadenylation signals, other untranslated nucleic acid sequences, transit or targeting sequences, selectable or classifying markers, promoters, enhancers and operators. Construct and vectors may also contain a gene without promoter that can use an endogenous promoter in insertion. Nucleic acid molecules that can be used in plant transformation or transfection can be any of the nucleic acid molecules of the present invention. It is not intended that the present invention be limited to the illustrated embodiments. Exemplary nucleic acid molecules have been described in part A of the detailed description, and exemplary additional non-limiting nucleic acid molecules are described below and illustrated in Figures 1-4, and in the examples. Referring now to the accompanying drawings, embodiments of the nucleic acid molecule of the present invention are shown in Figures 1-4. As described above, the nucleic acid molecule comprises (a) a first set of DNA sequences and (b) a second set of DNA sequences, which are located in one or more regions of T-DNA, each of the which is flanked by a right edge and a left edge. Within the T-DNA regions the transcription direction is shown by arrows. The nucleic acid molecules described can have their DNA sequences arranged in monocistronic or polycistronic configurations. Preferred configurations include a configuration in which the first set of DNA sequences and the second set of DNA sequences are located in a single T-DNA region. In each of the illustrated embodiments, the first set of DNA sequences comprises one or more sequences which when They are capable of selectively reducing one, two or all of the proteins and transcripts encoded by a gene selected from the group consisting of FAD2, FAD3 and FATB. Preferably each sequence in the first set of DNA sequences is capable, when expressed, of suppressing the expression of a different gene, including without limitation different gene family members. The sequences may include coding sequences, intron sequences, 3'UTR sequences, 5'UTR sequences, other coding sequences or any combination of the above. The first set of DNA sequences can be expressed in any available form, including as a dsRNA construct, a sense cosuppression construct, or as an antisense construct. The first set of DNA sequences is operably linked to at least one promoter that drives the expression of the sequences, which may be any functional promoter in a plant, or any plant promoter. Preferred promoters include, but are not limited to, a napin promoter, a 7Sa promoter, a 7Sa 'promoter, an arcelin promoter, or an FAD2-1A promoter. The second set of DNA sequences comprises coding sequences, each of which is a DNA sequence encoding a sequence that when expressed is capable of increasing one or both of the protein and transcript encoded by a gene selected from the group that consists of KAS I, KAS IV, delta 9 desaturase, and CP4 EPSPS. Each coding sequence is associated with a promoter, which can be any functional promoter in a plant, or any plant promoter.

Preferred promoters for use in the second set of DNA sequences are an FMV promoter and / or specific seed promoters. Preferred specific seed promoters, include, but are not limited to, a napin promoter, a 7Sa promoter, a 7Sa promoter, an arcelin promoter, a delta-9 desaturase promoter; or a FAD2-1A promoter. In the embodiments depicted in Figures 1 and 2, the first set of DNA sequences, when expressed, is capable of forming a dsRNA molecule that is capable of suppressing the expression of one or both of the protein and transcript encoded by, or transcribed from, a gene selected from the group consisting of FAD2, FAD3 and FATB. The first set of DNA sequences depicted in Figure 1 comprises three non-coding sequences, each of which expresses an RNA sequence (not shown) that exhibits identity for a non-coding region of a gene selected from the group that consists of FAD2, FAD3 and FATB genes. The non-coding sequences each express an RNA sequence exhibiting at least 90% identity for a non-coding region of a gene selected from the group consisting of FAD2, FAD3 and FATB genes. The first set of DNA sequences also comprises three antisense sequences, each of which expresses an antisense RNA sequence (not shown) which is capable of forming a double stranded RNA molecule with its respective corresponding RNA sequence (as is expressed by non-coding sequences). The non-coding sequences can be separated from the antisense sequences by a spacer sequence, preferably one that promotes the formation of a dsRNA molecule. Examples of such spacer sequences include those set forth in Wesley et al., Plant J., 27 (6): 581-90 (2001), and Hamilton et al., Plant J., 75: 737-746 (1988). In a preferred aspect, the spacer sequence is capable of forming a hairpin structure as illustrated in Wesley et al., Supra. Spacer sequences preferred particularly in this context with plant introns or their parts. A particularly preferred vegetable intron is an intron that can be spliced. Introns that can be spliced include but are not limited to, an intron selected from the group consisting of intron PDK, intron # 5 FAD3- 1A or FAD3- 1B, intron # 1 FAD3, intron # 3A FAD3, intron # 3B FAD3, intron # 3C FAD3, intron # 4 FAD3, intron # 5 FAD3, intron # 1 FAD2, and intron FAD2 -2. Other preferred spliced introns include, but are not limited to, an intron selected from the group consisting of intron # 1 FAD3, intron # 3A FAD3, intron # 3B FAD3, intron # 3C FAD3, and intron # 5 FAD3. Other preferred spliced introns include, but are not limited to, a spliceable intron that is about 0.75 kb to about 1.1 kb in length and is capable of facilitating an RNA hairpin structure. A non-limiting example of a particularly preferred splice intron is intron # 5 FAD3. The non-coding, sense-oriented molecules can optionally be separated from the corresponding antisense oriented molecules by a DNA spacer segment. The segment Spacer can be a gene fragment or artificial DNA. The spacer segment may be short to facilitate the formation of hairpin dsRNAs or so long as to facilitate dsRNA without a hairpin structure. The spacer can be provided by extending the length of one of the sense or antisense molecules as described in US 2005/0176670. Alternatively, a right-edge-right-edge sequence ("RB-RB") can be created after insertion into the plant genome as described in the U.S. patent application. 2005/0183170. Referring now to Figure 1, the nucleic acid molecule comprises two regions of T-DNA, each of which is flanked by a right border and a left border. The first T-DNA region comprises the first set of DNA sequences that is operably linked to a promoter, and the first T-DNA region further comprises a first part of the second set of DNA sequences comprising a first operably linked promoter. to a first coding sequence, and a second promoter operably linked to a second coding sequence. The second T-DNA region comprises a second part of the second set of DNA sequences comprising a third promoter operably linked to a third coding sequence. In a preferred embodiment shown in Figure 2, the nucleic acid molecule comprises a unique T-DNA region, which is flanked by a right border and a left border. The first and second sets of DNA sequences are all located in the region of Unique T-DNA. In the embodiments that express dsRNAs shown in Figures 1 and 2, the order of the sequences can be alternating from the illustrated and described, however the non-coding sequences and the antisense sequences are preferably arranged around the spacer sequence such that, when expressed, the first non-coding sequence can hybridize to the first antisense sequence, the second non-coding sequence can hybridize to the second antisense sequence, and the third non-coding sequence can hybridize to the third antisense sequence such that one molecule of single dsRNA can be formed. Preferably the non-coding sequences are in a sense orientation, and the antisense sequences are in an antisense orientation relative to the promoter. The numbers of non-coding, antisense and coding sequences, and their various relative positions in the T-DNA region (s) can also be altered in a manner suitable to achieve the objects of the present invention. Referring now to Figures 3 and 4, the illustrated nucleic acid molecule comprises a T-DNA region flanked by a right border and a left border, in which the first and second sets of DNA sequences are located. The first set of DNA sequences is operably linked to a promoter and a transcriptional termination signal. The second set of DNA sequences comprising a first promoter operably linked to a first sequence of encoding, a second promoter operably linked to a second coding sequence, and a third promoter operably linked to a third coding sequence. The transcriptional termination signal can be any functional transcriptional termination signal in a plant, or any plant transcriptional termination signal. Preferred transcriptional termination signals include, but are not limited to, an E9 3 'Rubisco pea sequence, a 3' napin Brassica sequence, a 3 'tml sequence and a 3' nos sequence. In the embodiment depicted in Figure 3, the first set of DNA sequences, when expressed, is capable of forming a sense cosuppression construct that is capable of suppressing the expression of one or more encoded proteins or transcripts., or derivatives of, a gene selected from the group consisting of FAD2, FAD3, and FATB. The first set of DNA sequences comprises three non-coding sequences, each of which expresses an RNA sequence (not shown) that exhibits sufficient identity for one or more non-coding regions of a gene selected from the group consisting of genes FAD2, FAD3 and FATB. The non-coding sequences each express an RNA sequence exhibiting at least 90% identity for one or more non-coding regions of a gene selected from the group consisting of FAD2, FAD3 and FATB genes. The order of the non-coding sequences within the first set of DNA sequences can be altered from that illustrated and described herein, but the non-coding sequences are arranged in a meaning sense relative to the promoter. Figure 4 depicts an embodiment in which the first set of DNA sequences, when expressed, is capable of forming an antisense construct that is capable of suppressing the expression of one or more proteins or transcripts encoded by, or derived from, a gene selected from the group consisting of FAD2, FAD3, and FATB. The first set of DNA sequences comprises three antisense sequences, each of which expresses an antisense RNA sequence (not shown) that exhibits identity for one or more non-coding regions of a gene selected from the group consisting of FAD2 genes, FAD3, and FATB. The antisense sequences each express an antisense RNA sequence that exhibits at least 90% identity for one or more non-coding regions of a gene selected from the group consisting of FAD2, FAD3, and FATB genes. The order of the antisense sequences within the first set of DNA sequences can be altered from that illustrated and described herein, but the antisense sequences are arranged in an antisense orientation relative to the promoter. The nucleic acid molecules described above are preferred embodiments which achieve the objects, aspects and advantages of the present invention. It is not intended that the present invention be limited to the illustrated embodiments. The arrangement of the sequences in the first and second sets of DNA sequences within the nucleic acid molecule is not limited to the arrangements illustrated and described, and can be altering in any suitable manner to achieve the objects, aspects and advantages of the present invention as described herein and illustrated in the accompanying drawings.

E. Transgenic Organisms, and Methods for Producing Them Any of the nucleic acid molecules and constructs of the invention can be introduced into a plant or plant cell in a permanent or transient handle. Preferred nucleic acid molecules and constructs of the present invention are described above in parts A through D of the detailed description, and in the examples. Another embodiment of the invention is directed to a method of producing transgenic plants which generally comprises the steps of selecting a suitable plant or plant cell, transforming the plant or plant cell with a recombinant vector, and obtaining a transformed host cell. . In a preferred embodiment the plant or cell is, or is derived from, a plant involved in the production of vegetable oils for edible and industrial uses. Especially preferred are temperate oilseed crops. Plants of interest include, but are not limited to, nabina (high erucic acid and cañola varieties), corn, soybeans, crambe, mustard, castor, peanut, sesame, cotton, linseed, safflower, oil palm, flax, sunflower, and coconut. The invention is applicable to monocotyledonous or dicotyledonous species in the same way, and will be easily applicable to new and / or improved transformations and regulatory techniques. Methods and technology for the introduction of DNA into plant cells are well known to those of skill in the art, and virtually any method by which nucleic acid molecules can be introduced into a cell is suitable for use in the present invention. Non-limiting examples of suitable methods include: chemical methods; physical methods such as microinjection, electroporation, the gene gun, microprojectile bombardment, and vacuum infiltration; viral vectors; and mechanisms mediated by receiver. Other methods of cell transformation can also be used to include but are not limited to the introduction of DNA into plants by direct DNA transferred into pollen, by direct injection of DNA into reproductive organs of a plant, or by direct injection of DNA into plants. immature embryo cells followed by rehydration of dissected embryos. Agrobacterium-mediated transfer is a widely applicable system for the introduction of genes into plant cells. See, for example, Fraley et al., Bio / Technology 3: 629-635 (1985); Rogers et al., Methods Enzymol. 153: 253-277 (1987). The region of DNA to be transferred is defined by the border sequences and DNA intervention is usually inserted into the plant genome. Spielmann et al., Mol. Gen. Genet. 205: 34 (1986). Modern Agrobacterium transformation vectors are capable of replication in E. coli as well as Agrobacterium, which is allowed for convenient manipulations. Klee et al., In: Plant DNA Infectious Agents, Hohn and Schell (eds.), Springer-Verlag, New York, pp. 179-203 (1985). The regeneration, development and cultivation of protoplast transformant plants of single plant or of several transformed explants is well known in the art. See, generally Maliga et al., Methods in Plant Molecular Biology, Cold Spring Harbor Press (1995); Weissbach and Weissbach, In: Methods for Plant Molecular Biology, Academic Press, San Diego, CA (1988). The plants of the present invention may be part of or generated from a breeding program, and may also be reprod using apomixis. Methods for the production of apomictic plants are known in the art. See, for example, the U.S. Patent. 5.81 1, 636. In a preferred embodiment, a plant of the present invention which includes nucleic acid sequences which when expressed are capable of selectively reducing the level of an FAD2, FAD3 and / or FATB protein, and / or an FAD2, FAD3 transcript, and / or FATB is crossed with another plant of the present invention that includes nucleic acid sequences which are expressed with the ability to over-expression with another enzyme. Preferably the other enzyme is selected from the group consisting of beta-ketoacyl-ACP synthase I, beta-ketoacyl-ACP synthase IV, delta-9 desaturase, and CP4 EPSPS. In another aspect, a plant of the present invention can be crossed with another plant that is transgenic or non-transgenic. A plant of the present invention can be crossed with another plant having a composition of oil containing modified levels of fatty acids, for example without limitation, a variety with an oil composition having a lower level of linolenic acid. In a preferred embodiment, a plant of the present invention is crossed with a variety with less than 3% by weight of linolenic acid, or in another embodiment, a plant of the present invention is crossed with another plant having more than 20% by weight. weight of stearic acid. Such plants which have modified levels of fatty acids are known in the art and are described, for example, in Hawkins and Kridl (1998) Píant Journal 13 (6): 743-752 and U.S. Pat. No. 6,365,802.

F. Products of the present invention The plants of the present invention may be used whole or in part. Preferred plant parts include reproductive or storage parts. The term "plant parts" as used herein includes, without limitation, seed, endosperm, ovule, pollen, roots, tuber, stems, leaves, logs, fruit, berries, nuts, bark, pods, seeds and flowers. In a particularly preferred embodiment of the present invention, the plant part is a seed. Any of the plants or their parts of the present invention can be processed to proda food, food, protein or oily preparation. In a preferred embodiment of the present invention may be a plant of the present invention having an oil with a fatty acid composition of the present invention. A part of the plant particularly preferred for this purpose is a seed. In a preterm embodiment the food, food, protein or oily preparation is designed for cattle, fish or human animals or any combination. Methods for producing food, food, protein and oily preparations are known in the art. See, for example, US Patents. 4,957,748, 5,100,679, 5,219,596, 5,936,069, 6,005,076, 6,146,669, and 6,156,227. In a preferred embodiment, the protein preparation is a high protein preparation. Said high protein content preparation preferably has a protein content of more than 5% w / v, more preferably 10% w / v, and even more preferably 15% w / v. In a preferred oily preparation, the oily preparation is a high oil content preparation with an oil content derived from a plant or part thereof of the present invention of more than 5% w / v, more preferably 10% w / v v, and even more preferably 15% w / v. In one embodiment, the oily preparation is a liquid and of a volume of more than 1.5, 10 or 50 liters. The present invention is provided for oil produced from plants of the present invention or generated by a method of the present invention. Said oil may exhibit increased oxidative stability. Also, said oil may be a minor or major component of any resulting product. In addition, said oil can be mixed with other oils. In a preferred embodiment, the oil produced from plants of the present invention or generated by a method of the present invention constitutes more 0.5%, 1%, 5%, 10%, 25%, 50%, 75% or 90% in volume or weight of the oily component of any product. In another embodiment, the oily preparation can be mixed and can constitute more than 10%, 25%, 35%, 50% or 75% of the mixture by volume. The oil produced from a plant of the present invention can be mixed with one or more organic solvents or petroleum distillates. The seeds of the plants can be placed in a container. As used herein, a container is any object capable of maintaining said seeds. A container preferably contains more than about 500, 1, 000, 5,000 or 25,000 seeds where at least about 10%, 25%, 50%, 75% or 100% of the seeds are derived from a plant of the present invention. The present invention also provides a container of about 10,000, more preferably about 20,000 and even more preferably about 40,000 seeds where about 10%, more preferably about 25%, more preferably 50% and even more preferably about 75% or 90% of the seeds are seeds derived from a plant of the present invention. The present invention also provides a container of about 10 kg, more preferably about 25 kg, and even more preferably about 50 kg of seeds where about 10%, more preferably about 25%, more preferably about 50% and even more preferably about 75% or 90% of the seeds are seeds of a plant of the present invention.

G. Oily compositions For many oily applications, saturated fatty acid levels are preferably less than 8% by weight, and more preferably approximately 2-3% by weight. Saturated fatty acids have high melting points which are undesirable in many applications. When used as a raw material or fuel, saturated fatty acids cause turbidity at low temperatures, and confer poor cold flow properties such as pour points and cold filter plugging points for fuel. Oil products that contain low saturated fatty acid levels may be preferred by consumers and the food industry because they are perceived as healthy and / or can be labeled "free of saturated fat" according to FDA guidelines. In addition, low saturated oils reduce or eliminate the need to winterize oil for food applications such as salty oils. In biodiesel and lubricant applications, oils with low saturated fatty acid levels confer improved cold flow properties and do not cloud at low temperatures. The factors that govern the physical properties of a particular oil are complex. Palmitic acid, stearic acid and other saturated fatty acids are usually solid at room temperature, in contrast to unsaturated fatty acids, which remain liquid. Because the saturated fatty acids do not have double bonds in the acyl chain, they remain stable for oxidation at elevated temperatures. Saturated fatty acids are important components in margarine and chocolate formulations, but for many food applications, reduced levels of saturated fatty acids are desired. Oleic acid has a double bond, but is relatively stable at high temperatures, and oils with high levels of oleic acid are suitable for cooking and other procedures where heating is required. Recently, the increased consumption of high oleic oils has been recommended, because oleic acid appears to lower blood levels of low density lipoproteins ("LDL") without affecting the high density lipoprotein ("HDL") levels. However, some limitation of oleic acid levels is desirable, because when oleic acid degrades at high temperatures, it creates negative taste compounds and decreases the positive flavors created by the oxidation of linoleic acid. Neff et al., JAOCS, 77: 1303-1313 (2000); Warner et al., J. Agrie. Food Chem. 49: 899-905 (2001). Preferred oils have oleic acid levels that are 65-85% or less by weight, in order to limit abnormal flavors in food applications such as frying oil and fried foods. Other preferred oils have oleic acid levels that are greater than 55% by weight in order to improve oxidative stability. Linoleic acid is a major polyunsaturated fatty acid in foods and is an essential nutrient for humans. It is a component desirable for many food applications because it is a major precursor of fried food flavor substances such as 2,4-decadienal, which makes good taste in fried foods. However, linoleic acid has limited stability when heated. Preferred food oils have levels of linoleic acid that are 10% or more by weight, to increase the formation of desirable fried food flavoring substances and are also 25% or less by weight, so that the formation of abnormal flavors is reduced. Linoleic acid also has cholesterol lowering properties, although dietary excess can reduce the ability of human cells to protect themselves from oxidative damage, thereby increasing the risk of cardiovascular disease. Toborek et al., Am J. Clin. J. 75: 1 19-125 (2002). See generally Flavor Chemistry of Lipid Foods, editors D.B. Min & T.H. Smouse, Am Oil Chem. Soc, Champaign, IL (1989). Linoleic acid has a lower melting point than oleic acid, it also contributes to improving the desirable cold flow properties in biodiesel and biolubricant applications. Preferred oils for more applications have linoleic acid levels of 30% or less by weight, because the oxidation of linoleic acid limits the useful storage or time of use of the oil for frying, food, food, and lubricating products. See generally Physical Properties of Fats, Oils, and Emulsifiers, ed. N. Widlak, AOCS Press (1999); Erhan & Asadauskas, Lubricant Basestocks from Vegetable Oils, Industrial Crops and Products, 1 1: 277-282 (2000). In addition, high levels of linoleic acid in livestock feed can lead to undesirable high levels of linoleic acid in the milk of the daily cattle, and therefore oxidative stability and deficient flavor. Timmons et al., J. Dairy Sci. 84: 2440-2449 (2001). An oily composition useful widely has linoleic acid levels of 10-25% by weight. Linolenic acid is also an important component of the human diet. It is used to synthesize the? -3 family of long chain fatty acids and the prostaglandins derived from them. However, their double bonds are highly susceptible to oxidation, so that oils with high levels of linolenic acid deteriorate rapidly on exposure to air, especially at high temperatures. Partial hydrogenation of such oils is often necessary before they can be used in food products to retard the formation of abnormal flavors and rancidity when the oil is heated, but hydrogenation creates unhealthy trans fatty acids that contribute to cardiovascular disease. To achieve improved oxidative stability, and reduce the need for hydrogenated oil, preferred oils have linolenic acid levels that are 8% or less by weight, 6% or less, 4% or less, less than about 3%, and more preferably 0.5. -2% by weight of the total fatty acids in the oil of the present invention. Soybean oil of the present invention can also be used as a mixing source to make a mixed oil product. By a mixing source, it means that the oil of a soybean seed of the present invention can be mixed with other oils vegetables to improve the characteristics, such as fatty acid composition, taste, and oxidative stability, of the other oils. The amount of oil of a soybean seed of the present invention that can be used will depend on the properties sought to be achieved in the resulting final mixed oil product. Examples of oil products include, but are not limited to, margarines, fat to make the pastry more friable, oils for frying, salty oils, etc. The oil of a soybean seed of the present invention may be a mixed oil, synthesized oil or in a preferred embodiment an oil generated from an oleaginase having an appropriate oily composition. An oil generated directly from a soybean seed is an unmixed oil. In another aspect, an oil is directly from a mature soybean seed. In this regard, a mature seed as defined by a seed that is harvested in the field for commercial agricultural practices, such as sale for food. In a preferred embodiment, the oil is a soybean oil. The oil can be a crude oil such as crude soybean oil, or it can be a processed oil, for example the oil can be refined, bleached, deodorized and / or conditioned for winter. As used herein, "refinement" refers to a process for treating natural or processed fat or oil to remove impurities, and can be performed by the treatment of grease or oil with caustic soda, followed by centrifugation, washing with water, and heating under vacuum. "Whitening" refers to a procedure for the treatment of fat or oil to remove or reduce levels of coloring materials in the grease or oil. Whitening can be done by treating fat or oil with activated charcoal or fuller's earth (diatomaceous earth). "Deodorization" refers to a process of removing components of a grease or oil that contributes objectionable flavors or odors to the final product, and can be accomplished by the use of high vacuum and superheated steam washing. "Conditioning for winter" refers to a procedure for removing saturated glycerides from an oil, and can be performed by cooling and removing from the solidified portions of an oil fat. A preferred oil of the present invention has a low saturated oily composition, or a zero saturated oily composition. In other preferred embodiments, oils of the present invention have increased levels of oleic acid, reduced saturated fatty acid levels, and reduced polyunsaturated fatty acid levels. In further preferred embodiments, oils of the present invention have increased levels of oleic acid and reduced saturated fatty acid levels. In a preferred embodiment, the oil is a soybean oil. The percentages of fatty acid content, or fatty acid levels, used herein refer to percentages by weight. In a first embodiment, an oil of the present invention preferably has an oily composition that is 55 to 80% oleic acid, about 12 to 43% polyunsaturated, and 2 to 8% saturated fatty acids, more preferably has an oily composition What is it 55 to 80% oleic acid, approximately 1 to 42% polyunsaturated, and 3 to 6% saturated fatty acids; and even more preferably has an oily composition which is 55 to 80% oleic acid, approximately 16.5 to 43% polyunsaturated, and 2 to 3.6% saturated fatty acids. In a second embodiment, an oil of the present invention preferably has an oily composition that is 65 to 80% oleic acid, about 12 to 33% polyunsaturated, and 2 to 8% saturated fatty acids, more preferably has an oily composition which is 65 to 80% oleic acid, approximately 14 to 32% polyunsaturated, and 3 to 6% saturated fatty acids; and even more preferably has an oily composition that is 65 to 80% oleic acid, about 6.5 to 33% polyunsaturated, and 2 to 3.6% saturated fatty acids. In a third embodiment, an oil of the present invention preferably has an oily composition that is about 42 to about 85% oleic acid and about 8 to about 1.5% saturated fatty acids, more preferably the oily composition furthermore has an amount combined oleic acid and linoleic acid equaling about 65% to about 95% by weight of the total oily composition. Even more preferably the oily composition of the present invention has a combined amount of oleic acid and linoleic acid equaling from about 75% to about 90%, about 75% to about 95%, about 75% to about 85%, about 65% a about 90%, about 70% to about 90% by weight of the total oily composition. In a fourth embodiment, an oil of the present invention has an oily composition that is about 42 to about 85% oleic acid, about 8% to about 1.5% saturated fatty acids, about 6% to about 15% by weight of linoleic acid; more preferably it has an oily composition which is about 42 to about 85% oleic acid, about 8% to about 1.5% saturated fatty acids, less than 35% by weight linoleic acid, and even more preferably has an oily composition which is about 42% to about 85% oleic acid, about 8% to about 1.5% saturated fatty acids, about 9% by weight of linoleic acid. In a fifth embodiment, an oil of the present invention has an oily composition that is about 50% to about 85% oleic acid and about 8% to about 1.5% saturated fatty acids; more preferably about 50% to about 85% oleic acid, about 8% to about 1.5% saturated fatty acids, about 4% to about 14% by weight of linolenic acid; more preferably it has an oily composition which is about 50% to about 85% oleic acid, about 8% a about 1.5% of saturated fatty acids, less than about 35% by weight of linolenic acid; and even more preferably has an oily composition which is about 42 to about 85% oleic acid, about 8% to about 1.5% saturated fatty acids, about 2% to about 45% by weight of linolenic acid. In another embodiment, an oil of the present invention has an oily composition that is about 65-80% oleic acid, about 3-8% saturated, and about 12-32% polyunsaturated. In another embodiment, an oil of the present invention has a composition that is about 65-80% oleic acid, about 2-3.5% saturates, and about 16.5-33% polyunsaturates. In a particularly preferred embodiment, an oil of the present invention has an oily composition that is about 47-83% oleic acid and about 5% saturated; about 60-80% oleic acid and about 5% saturated; approximately 50.85% oleic and approximately 2-7% saturated; about 55-85% oleic acid and about 2.5-7% saturates; about 47-88% oleic acid and about 3-7% saturated; about 43-85% oleic acid and about 5-7% saturates; about 81 -85% oleic acid and about 5% saturates; Approximately 74-83% of oleic acid and about 6% saturates; about 65-87% oleic acid and about 6% saturates; about 66-80% oleic acid and about 6% saturates; about 42-77% oleic acid and about 5-8% saturates; about 60-77% oleic acid and about 6% saturates; about 70-81% oleic acid and about 5-7% saturates; about 52-71% oleic acid and about 5-7% saturates; about 44-71% oleic acid and about 6% saturates; about 61 -71% oleic acid and about 8% saturated; about 57-71% oleic acid and about 7% saturates; about 23-58% oleic acid and about 8-14% saturated; about 20-70% oleic acid and about 6% saturates; about 21 -35% oleic acid and about 5-6% saturated; or about 19-28% oleic acid and about 5% saturates. In other embodiments, the percentage of oleic acid is 50% or greater, 55% or greater; 60% or greater; 65% or greater; 70% or greater; 75% or greater; u 80% or greater; or is in a range of 50 to 80%; 55 to 80%; 55 to 75%; 55 to 65%; 60 to 85%; 60 to 80%; 60 to 75%; 60 to 70%; 65 to 85%; 65 to 80%; 65 to 70%; 65 to 70%; or 69 to 73%. Suitable percent ranges for the oleic acid content in oils of the present invention also include ranges in which the lower limit is selected from the following percentages: 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73 , 74, 75, 76, 77, 78, 79, or 80 percent; and the upper limit is selected from the following percentages: 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75, 76, 77, 78, 79 , 80, 81, 82, 83, 84, 85, 86, 87, 88, 89 or 90 percent. In these other embodiments, the percentage of linoleic acid in an oil of the present invention is a range of 10 to 40%; 10 to 39% >; 10 to 30%; 10 to 29%; 10 to 28%; 10 to 25%; 10 to 21%; 10 to 20%; 1 1 to 30%; 12 to 30%; 15 to 25%; 20 to 25%; 20 to 30%; or 21 to 24%. The ranges of percentages suitable for the linoleic acid content in oils of the present invention also include ranges in which the lower limit is selected from the following percentages: 10, 1, 2, 13, 14, 15, 16, 17, 18 , 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29 or 30 percent; and the upper limit is selected from the following percentages: 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39 or 40 percent. In these other embodiments, the percentage of linolenic acid in an oil of the present invention is 10% or less, 9% or less, 8% or less; 7% or less; 6% or less; 5% or less; 4.5% or less; 4% or less; 3.5% or less; 3% or less; 3.0% or less; 2.5% or less; or 2% or less; or it is a range of 0.5 to 2%; 0.5 to 3%; 0.5 to 4.5%; 0.5% to 6%; 3 to 5% 3 to 6%; 3 to 8%; 1 to 2%; 1 to 3%; or 1 to 4%. In these other embodiments, the percentage of saturated fatty acids in an oily composition of the present invention is 15% or less; 14% or less; 13% or less; 12% or less; eleven % 0 less; 10% or less; 9% or less; 8% or less; 7% or less; 6% or less; 5% or less; 4% or less; or 3.6% or less; or is a range of 2 to 3%; 2 to 3.6%; 2 to 4%; 2 to 8%; 3 to 15%; 3 to 10%; 3 to 8%; 3 to 6%; 3.6 to 7%; 5 to 8%; 7 to 10%; or 10 to 15%. In other embodiments, saturated fatty acids in an oil of the present invention include the combination of palmitic and stearic fatty acids. In one embodiment, the percentage of saturated fatty acids varies from about 10% or less; approximately 9% or less; about 8% or less; approximately 7% or less; about 6% or less; about 5% or less; approximately 4.5% or less; about 4% or less; approximately 3.5% or less; about 3% or less; approximately 3.0% or less; approximately 2.5% or less; or about 2% or less; or it is a range of 0.5 to 2%; 0.5 to 4.5%; 0.5 to 6%; 0.5 to 7%; 0.5 to 8%, 0.5 to 9%; 1 to 4%, 1 to 5%; 1 to 6% 1 to 7%; 1 to 8%; 1 to 9%; 1.5 to 5%; 1.5 to 6%; 1.5 to 7%; 1.5 to 8%; 1.5 to 9%; 2 to 5%; 2 to 6%; 2 to 7%; 2 to 8%; 2 to 9%; 3 to 5%; 3 to 6%; 3 to 7%; 3 to 8%; 3 to 9%; 4 to 7%; 4 to 8%; 4 to 9%; 5 to 7%; 5 to 8%; and 5 to 9%. In these embodiments, suitable percentage ranges for saturated fatty acid content in oils of the present invention also include ranges in which the lower limit is selected from the following percentages: 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5, 5, 5.5, 6 or 6.5 percent; and the upper limit is selected from the following percentages: 1 1, 10, 9, 8, 7, 6, 5, 4.5, 4, 3.5, 3, 2.5, 2, 1 .5, 1, or 0.5 percent.

In other embodiments, the percentage of palmitic fatty acid in an oily composition of the present invention varies from 6% or less; 5% or less; 4.5% or less; 4% or less; 3.5% or less; 3% or less; 3.0% or less; 2.5% or less; or 2% or less; or it is a range of 0.5 to 2%; 0.5 to 3%; 0.5 to 4.5%; 0.5 to 6%; 1 to 3%; 1 to 4%; 1 to 5%; 1 to 6%; 1.5 to 2%; 1.5 to 3%; 1.5 to 4%; 1.5 to 4.5%; 1.5 to 5%; 1.5 to 5.5%; 1.5 to 6%; 1.5 to 6.5%; 1.5 to 7%; 2 to 3%; 2 to 3.5%; 2 to 4%; 2 to 4.5%; 2 to 5%; 2 to 6%; 2 to 7%; 2 to 8%; 3 to 5%; 3 to 6; 3 to 7%, 3 to 8%; 3 to 9%. In these embodiments, suitable percentage ranges for linoleic acid content in oils of the present invention also include ranges in which the lower limit is selected in the following percentages: 0.5, 1, 1.5, 2, 2.5, 3, 3.5 , 4, 4.5, 5, 5.5, 6, 6.5, 7 or 7.5 percent; and the upper limit is selected from the following percentages: 1 1, 10, 9, 8, 7, 6, 5, 4.5, 4, 3.5, 3 or 2 percent. In other embodiments, the percentage of stearic fatty acid in an oily composition of the present invention varies from 3% or less; 3.0% or less; 2.5% or less; or 2% or less; or it is a range of 0.5 to 1%; 0.5 to 1.5%; 0.5 to 2%; 0.5 to 2.5%; 0.5 to 3%; 0.5 to 4%; 1 to 2%; 1 to 3%; 1 to 4%; 1.5 to 2%; 1.5 to 3% or 1.5 to 4%. In these embodiments, suitable percentage ranges for linoleic acid content in oils of the present invention also include ranges in which the lower limit is selected from the following percentages: 0.5, 1, 1.5, 2, 2.5, 3 or 3.5 percent; and the upper limit is selected from the following percentages: 3.5, 3, 2.5, 2 or 1.5.

An oil of the present invention is particularly suitable for use as an oil for cooking or frying. Due to its reduced polyunsaturated fatty acid content, the oil of the present invention does not require excessive processing of customary oils because some objectionable odors and coloring compounds are present. In addition, the low saturated fatty acid content of the present oil improves the cold flow properties of the oil, and obviates the need to heat the stored oil to prevent it from crystallization or solidification. The improved cold flow also increases drainage of the oil from the fried food material once it has been removed from the frying oil, resulting in a lower fat product. See Bouchon et al., J. Food Science 66: 918-923 (2001). the low levels of linolenic acid in the present oil are particularly advantageous in frying to reduce abnormal flavors. The present oil is also well suited for use as a salty oil (an oil that maintains clarity at refrigerator temperatures of 40-50 degrees Fahrenheit). Its clarity improves at refrigeration temperatures, due to its low saturated fatty acid and moderate linoleic acid content, reducing or eliminating the need to winterize the oil before use as a salty oil. In addition, the low linoleic and low linoleic acid content of the present oil makes it well suited for the production of fat to make the most friable pastry, margarine and other semi-solid vegetable fats used in foods. The production of these fats involves usually hydrogenation of unsaturated oils such as soybean oil, corn oil, or canola oil. The increased oxidation and flavor stability of the present oil means that it does not need to be hydrogenated to the extent that the typical vegetable oil is for uses such as margarine and fat to make the pastry more friable, thereby reducing the processing costs and the production of unhealthy trans isomers. An oil of the present invention is also suitable for use as a raw material for producing biodiesel, particularly since the biodiesel made from said oil has improved cold flow, improved ignition quality (cetane number), improved oxidative stability, and emissions of reduced nitric oxide. Biodiesel is an alternative diesel fuel usually comprised of methyl esters of C16-C22 saturated, monounsaturated and polyunsaturated fatty acids. The cetane number is a measure of ignition quality - the shortest ignition delay time of fuel in the engine, the highest cetane number. The ASTM standard specification for biodiesel fuel (D 6751 -02) requires a minimum cetane number of 47. The use of biodiesel in conventional diesel engines results in substantial reductions of contaminants such as sulfates, carbon monoxide and particulates compared to fuel of diesel oil, and the use in school buses can greatly expose children to toxic diesel exhaust gases. A limitation for the use of 100% conventional biodiesel as fuel is the point of high turbidity of conventional soybean biodiesel (2 degrees C) compared to diesel number 2 (-16 degrees C). Dunn et al., Recent. Res. Devel. in OH Chem., 1: 31 -56 (1997). The biodiesel made from the oil of the present invention has a (reduced) cloud point and improved cold filter plugging point, and can also be used in blends to improve the cold temperature properties of biodiesel made from highly saturated but inexpensive sources. fat such as animal fats (bait, lard, chicken fat) and palm oil. Biodiesel can also be mixed with petroleum diesel at any level. Biodiesel is typically obtained by the extraction, filtration and refining of soybeans to remove free fats and phospholipids, and then transesterification of the oil with methanol to form methyl esters of fatty acids. See, for example, the U.S. Patent. No. 5,891, 203. The resulting soy methyl esters are commonly referred to as "biodiesel". The oil of the present invention can also be used as a diesel fuel without the formation of methyl esters, such as, for example, the acetal mixtures with the oil. See, for example, the patent of E.U.A. No. 6,013.1 14. Due to its improved cold flow and oxidative stability properties, the oil of the present invention is also useful as a lubricant, and as a diesel fuel additive. See, for example, the Patents of E.U.A. Nos. 5,888,947, 5,454,842 and 4,557,734. Soybeans and oils of the present invention are also suitable for use in a variety of soy foods made from whole soybeans, such as soy milk, soy nut butter, natto, and tempeh, and soy foods made from processed soybeans and soybean oil. soybeans, which include soybean meal, soybean meal, soy protein concentrate, soy protein isolates, textured soy protein concentrate, hydrolyzed soy protein, chantilly custard, cooking oil, salty oil, fat to make the most friable pastry and lecithin. Whole soybeans are also edible, and are usually sold to raw, toasted or edamame consumers. Soy milk is usually produced by wetting and crushing whole soybeans, it can be consumed as it is, spray dried or processed to form soy yogurt, tofu, tofu, or yuba. The soybean or oil present can be advantageously used in these and other soy foods because of its improved oxidative stability, the reduction of abnormal taste precursors, and its low saturated fatty acid level.

G. Suppression Modulation Another embodiment of the invention is directed to a method of modulating gene suppression levels. The modulation of gene suppression can result in more or less gene deletion. The deletion of a gene product can be the result of insertion of a construct of the present invention into a plant genome. Similarly, modulation of gene deletion can result from insertion of a construct of the present invention into a plant genome. Other examples of methods for modulating gene suppression include, without limitation, antisense techniques, co-suppression, RNA interference (RNAids), transgenic animals, hybrids, and ribozymes using a construct of the present invention. The following examples are provided by way of illustration, and are not intended to be limiting of the present invention. Deletion of a gene can be modulated by altering the length of the transcribable DNA used for deletion, whose sequence is derived from the gene targeted for suppression. Many methods can be used for suppression of a gene that uses post-transcriptional gene silencing mechanisms. Without being limited by theory, these methods are believed to have in common the expression of an RNA molecule that hybridizes to another RNA molecule. Surprisingly, it may be advantages to use an RNA molecule of particular lengths to modulate or moderate the suppression of steady state expression levels of an endogenous target gene. The deletion of FAD2-1 gene leads to high levels of oleic acid and reduction of linoleic acid levels. When FAD2-1 is heavily suppressed, oleic acid levels may be greater than 65%, which causes a reduction in palmitic acid and linoleic acid levels. For example, when FAD2-1 is suppressed, oleic acid levels can reach 85% and palmitic and stearic acid levels are reduced to approximately 10%. Similarly, the down regulation of FATB results in decreased levels of saturated fatty acids, primarily palmitate. When FAD2 and FATB are suppressed so that the levels are approximately 85%, the saturates levels are approximately 10%. When FAD2 and FATB are suppressed so that oleic levels are greater than 85%, saturated levels can fall below 10%. In view of the present invention, saturation levels can be reduced to less than 10% without increasing the oleic acids above 85%. In another embodiment, the suppression of FAD2 is modulated by reducing the intron length of FAD2-1 induced in the plant. Less suppression of FAD2 results at moderate levels of oleic acid, approximately 40-85% oleic acid. The deletion of FAD2 is reduced as the length of introduced FAD2-1 intron fragment is reduced. For example, an FAD2-1 intron reduced in length by at least 100 contiguous nucleotides can reduce the suppression of FAD2 and the corresponding increase in oleic acid and decrease in levels of linoleic acid. The relationship between the decrease in deletion of endogenous gene and decrease in length of homologous DNA can be determined empirically by introducing different DNA lengths. For example, the amount of reduction in suppression obtainable by reducing the length of homologous introduced DNA can be determined by deleting increased portions of homologous DNA that are introduced and assayed for target gene expression.

Included in the present invention is a method for moderating the suppression of FAD2 although it still has a strong reduction of saturated levels in a plant. In these plants, oleic acid levels can vary from 40-85%. Similarly, less than complete suppression of FATB occurs when the combined 3 'and 5' untranslated regions are introduced as compared to when the full-length FATB gene is introduced into a host cell. In a similar way, levels of FATB suppression are reduced when the 5 'part of the open reading frame, which encodes mainly the chloroplast transit peptide, is introduced into a host cell. In cells with deleted FAD2 and FATB using methods according to the present invention, the levels of oleic acid can be 40-85% although the saturated levels can be between 1 to 9 percent. In one embodiment, the present invention is directed to a method for modulating gene suppression to reduce deletion relative to the deletion of a whole gene element, where an entire gene element can be a whole gene, an entire exon, a whole intron, a whole signal sequence, or a whole UTR, then constructing a recombinant nucleic acid molecule comprising a fragment of the endogenous sequence of the gene element; initiating the expression of the recombinant nucleic acid molecule in a host cell; and the suppression of the endogenous gene with recombinant acid molecule. The gene that is deleted can be any gene, including FAD2 and FATB. In one modality, the present invention is directed to a method for modulating the suppression of FAD2 or FATB which comprises: expressing an element sequence of the FAD2 gene or partial FATB in host cell, wherein an element of the FAD2 or FATB gene is of an endogenous FAD2 or FATB gene in the host cell and an element sequence of the FAD2 or FATB gene can be an FAD2 or FATB gene, an FAD2 or FATB exon, an FAD2 intron or FATB, a coding region of the FAD2 transit peptide or FATB, or a FAD2 UTR or FATB; and the element sequence of the FAD2 gene or partial FATB is smaller than the element sequence of the FAD2 gene or whole FATB; and suppressing an endogenous FAD2 or FATB with the FAD2 gene element sequence or partial FATB, where the deletion levels of the endogenous FAD2 gene or FATB in the host cell are lower than the levels of endogenous gene FAD2 or FATB deletion in a cell host with a similar genetic background and a second FAD2 or FATB nucleic acid sequence comprising the element sequence of the FAD2 gene or whole FATB of the FAD2 or FATB gene element. In another embodiment, the present invention is directed to a method of altering the oily composition of a plant cell by transforming a plant cell with a recombinant nucleic acid molecule comprising a DNA sequence that suppresses the endogenous expression of FAD2, FATB or FAD2 and FATB where the DNA sequence comprises a nucleic acid sequence of FAD2, FATB or FAD2 and FATB that is shorter than the entire sequence of an entire genetic element selected from a gene, an exon, an intron, a region coding of transit peptide, about 3'UTR, about 5'-UTR and an open reading frame; and the growth of the plant cell under conditions where the transcription of said DNA sequence is initiated, whereby the oily composition is altered in relation to a plant cell with a similar genetic background but lacks the recombinant nucleic acid molecule. An element of the FAD2 or FATB gene can be shortened in length by 50, 75, 100, 150, 175, 200, 250, 300, 350, 400, 450, 500, 600, 800, 1000, 2000, 3000 or 4000 nucleotides . A length of an element of the FAD2 or FATB gene may be 50, 75, 100, 150, 175, 200, 220, 250, 300, 320, 350, 400, 420, 450, 500, 550, 600, 800 or 1000 nucleotides. In another embodiment, the present invention is directed to a method of increasing the content of oleic acid and reducing the content of saturated fatty acid in a plant seed by: i) shortening the length of an exogenous FAD2 DNA sequence in a host cell until the amount of FAD2 expression suppression of a transformed plant is at least partially reduced in relation to the suppression of FAD2 expression in host cell with a similar genetic background and an entire exogenous FAD2 gene DNA sequence; and ii) growth of a plant with a nucleic acid molecule comprising the shortened FAD2 DNA sequence, wherein the shortened FAD2 DNA sequence at least partially suppresses the endogenous expression of FAD2; and ii) cultivate a plant that produces seed with a reduced saturated fatty acid content in relation to the seed of a plant that has a similar genetic background but that lacks the shortened DNA FAD2 sequence. The amount that the exogenous FAD2 DNA sequence is shortened to at least partially reduce the suppression of endogenous FAD2 that can be determined empirically by introducing different lengths of DNA. For example, the amount of reduction in suppression obtainable by reducing the length of homologous introduced DNA can be determined by suppressing increased portions of homologous DNA that is introduced and assayed for expression of the target gene. The amount of FAD2 expression suppression can be obtained as an average of three or more, six or more, ten or more, fifteen or more, or twenty or more seeds of a plant. In another embodiment, the present invention is directed to a method of producing a transformed plant having seed with a reduced saturated fatty acid content by transforming a plant cell with a recombinant nucleic acid molecule comprising a DNA sequence that suppresses the endogenous expression of FAD2 and FATB, wherein the DNA sequence comprises an FAD2 nucleic acid sequence that is shorter than the entire sequence of a whole genetic element selected from a gene, an exon, an intron, a transit peptide coding region.; and some UTR; and the growth of the transformed plant, wherein the transformed plant produces seed with a reduced saturated fatty acid content in relation to the seed of a plant having a similar genetic background but lacking said recombinant nucleic acid molecule.

In another embodiment, the present invention is directed to a method of modulating the fatty acid composition of oil from a seed of a temperate oleaginous culture by isolating a genetic element of at least 40 nucleotides in length which is capable of suppressing the expression of an endogenous gene in the path of fatty acid synthesis; generating more than one shortened fragment of the genetic element; introducing each of more than one of the shortened fragments into a plant cell of the temperate oleaginous culture to produce transgenic plants; and selecting a transgenic plant comprising a shortened fragment of determined length and sequence that effects a desirable change in the fatty acid composition of seed oil. In a preferred embodiment, the above method also includes the construction of a recombinant DNA construct having at least two shortened fragments of two different different endogens that effect different desirable changes in the fatty acid composition of seed oil; introducing the recombinant DNA construct into a plant cell of the temperate oleaginous culture to produce transgenic plants; and selecting a transgenic plant comprising at least two shortened fragments and an oil fatty acid composition of a seed having more than one desirable change effected by at least two shortened fragments. In another embodiment, the present invention is directed to a soybean that exhibits an oil composition having a strongly reduced saturated fatty acid content and an oleic acid content. moderately increased having a DNA sequence that suppresses the endogenous expression of FAD2 in a host cell, where the DNA sequence has an FAD2 nucleic acid sequence that is shorter than the entire sequence of an entire genetic element selected from a gene , an exon, an intron, a transit peptide coding region, and UTR. The following examples are illustrative and are not intended to limit in any way. All publications, patents and patent applications mentioned in this specification are incorporated herein for reference to the same extent as if each individual publication, patent or patent application is specifically and individually indicated to be incorporated for reference.

EXAMPLES EXAMPLE 1 Isolation of FATB-2 sequences The leaf tissue is obtained from the Asgrow A3244 soybean variety, which grows in liquid nitrogen and is stored at -80 ° C until use. Six mi of SDS extraction pH regulator (650 ml of sterile ddH2O, 100 ml of 1 M Tris-CI with pH 8, 100 ml of EDTA 0.25M, 50 ml of 20% SDS, 100 ml of 5M NaCl, μ? of beta-mercaptoethanol) is added to 2 ml of tissue from frozen / crushed leaf, and the mixture is incubated at 65 ° C for 45 minutes. The sample is shaken every 15 minutes. 2 ml of 5 M potassium acetate cooled with ice is added to the sample, the sample is shaken, and then incubated on ice for 20 minutes. 3 ml of CHCL3 is added to the sample and the sample is stirred for 10 minutes. The sample is centrifuged at 10,000 rpm for 20 minutes, and the supernatant is collected. 2 ml of isopropanol are added to the supernatant and mixed. The sample is then centrifuged at 10,000 rpm for 20 minutes and the supernatant drained. The pellet is re-suspended in 200 μ? of RNase and incubated at 65 ° C for 20 minutes. 300 μ? of ammonium acetate / isopropanol (1: 7) and mixed. The sample is then centrifuged at 10,000 rpm for 15 minutes and the supernatant discarded. The pellet is rinsed with 500 μ? of 80% ethanol and allowed to air dry. The pellet of genomic DNA is then resuspended in 200 μ? of T10E1 (10 m Tris: 1 mM EDTA). A contiguous sequence of soy FATB-2 cDNA (SEO ID NO: 42) is used to designate thirteen oligonucleotides that extend the gene: F1 (SEQ ID NO: 48), F2 (SEO ID NO: 49), F3 (SEO ID) NO: 50), F4 (SEQ ID NO: 51), F5 (SEQ ID NO: 52), F6 (SEQ ID NO: 53), F7 (SEQ ID NO: 54), R1 (SEQ ID NO: 55), R2 (SEQ ID NO: 56), R3 (SEQ ID NO: 57), R4 (SEQ ID NO: 58), R5 (SEQ ID NO: 59), and R6 (SEQ ID NO: 60). Oligonucleotides are used in pairs for PCR amplification of isolated soybean genomic DNA: pair 1 (F1 + R1), pair 2 (F2 + R1), pair 3 (F3 + R2), pair 4 (F4 + R3), pair 5 (F5 + R4), par 6 (F6 + R5), and par 7 (F7 + R6). The PCR amplification of par 5 is carried out as follows: 1 cycle, 95 ° C for 10 minutes; 30 cycles, 95 ° C for 15 seconds, 43 ° C for 30 seconds, 72 ° C for 45 seconds; 1 cycle, 72 ° C for 7 minutes. For all other oligo pairs, PCR amplifications are carried out as follows: 1 cycle, 95 ° C for 10 minutes; 30 cycles 95 ° C for 15 seconds, 48 ° C for 30 seconds, 72 ° C for 45 seconds; 1 cycle, 72 ° C for 7 minutes. Positive fragments are obtained from the first pairs 1, 2, 4, 5, 6 and 7. Each fragment is cloned in vector pCR2.1 (Invitrogen). Fragments 2, 4, 5 and 6 are confirmed and sequenced. These four sequences are aligned to form a genomic sequence for the FATB-2 gene (SEQ ID NO: 43). Four introns are identified in the soybean FATB-2 gene by comparison of the genomic sequence to the cDNA sequence; intron 1 (SEQ ID NO: 44) the base 1 19 is expanded to the base 1333 of the genomic sequence (SEQ ID NO: 43) and is 1215 bp in length; intron II (SEQ ID NO: 45) expands base 2231 to base 2568 of the genomic sequence (SEQ ID NO: 43) and is 338 bp in length; intron III (SEQ ID NO: 46) expands base 2702 to base 3342 of the genomic sequence (SEQ ID NO: 43) and is 641 bp in length; and intron IV (SEQ ID NO: 47) expands base 3457 to base 3823 of the genomic sequence (SEQ ID NO: 43) and is 367 bp in length.

EXAMPLE 2 Suppression constructs 2A. Constructs FAD2-1 Intron # 1 FAD2-1 A (SEQ ID NO: 1) is cloned into the expression cassette, pCGN3892, in sense and antisense orientations. The vector pCGN3892 contains the soybean 7S promoter and a rbcS 3 'pea. Both gene fusions are then ligated separately into pCGN3892, a vector containing the CP4 EPSPS gene regulated by the FMV promoter. The resulting expression constructs (sense pCGN5469 and antisense pCGN5471) are used for the transformation of the soybean seed. Intron FAD2-1 B (SEQ ID NO: 2) is fused at the 3 'end of intron # 1 FAD2-1 A in plasmid pCGN5468 (contains the soybean 7S promoter fused to the FAD2-1 A intron ( sense) and a rbcS 3 'pea) or pCGN5470 (contains the soybean 7S promoter fused to the FAD2-1 A intron (antisense) and a rbcS 3' vetch) in sense and antisense orientation, respectively. The resulting intron combination fusions are then ligated separately into pCGN9372, a vector containing the CP4 EPSPS gene regulated by the FMV promoter. The resulting expression constructs (pCGN5485, intron sense FAD2-1 A & FAD2-1 B and intron antisense pCGN5486, FAD2-A &FAD2-1 B) are used for the transformation of soybean seed. 2B. Constructs FAD3-1 Intron # 1, # 2, # 4 and # 5 FAD3-1 (SEQ ID NOs: 7, 8, 10 and 1 1, respectively), intron # 3C FAD3-1 B (SEQ ID NO: 23) and # $ (SEQ ID NO: 24), are all linked separately in pCGN3892, in sense and antisense orientation. pCGN3892 contains the soybean 7S promoter and a 3b rbcS pea. These funsions are ligated into pCGN9372, a vector containing the CP4 EPSPS gene regulated by the FMV promoter for soybean transformation. The resulting expression constructs (pCGN5455, sense intron # 4 FAD3-1A, pCGN5459, antisense intron # 4 FAD3-1A, pCGN5456, sense intron # 5 FAD3, pCGN5460, antisense intron # 5 FAD3-A; pCGN5456, antisense intron # 2 FAD3-1A, pCGN5473, antisense intron # 1 FAD3-1 A) are used for the transformation of soybean seed. 2 C. Constructos FatB The intron sequence II FATB-1 of soybean (SEQ ID NO: 30) is amplified via PCR using a partial genomic clone FATB-1 as a template. The PCR amplification is carried out as follows: 1 cycle 95 ° C for 10 minutes, 25 cycles, 95 ° C for 30 seconds, 62 ° C for 30 seconds, 72 ° C for 30 seconds; 1 cycle, 72 ° C for 7 minutes. The PCR amplification results in a product that is 854 bp long, which includes restriction sites redesigned at both ends. The PCR product is cloned directly into the expression cassette pCGN3892 in sense orientation, as Xho sites designed at the 5 'end of the primers PCR, to form pMON70674. The vector pCGN3892 contains the soybean 7S promoter and a rbcS 3 'pea. pMON70674 is then cut with Noñ and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter. The resulting gene expression construct, pMON70678, is used for the transformation of soybean using Agrobacterium methods. Two other expression constructs containing the intron II FATB-1 sequence (SEQ ID NO: 30) are created. pMON70674 is cut with Noñ and ligated into pMON70675 containing the CP4 EPSPS gene regulated by the FMV promoter and the KAS IV gene regulated by the napin promoter, resulting in pMON70680. The expression vector pMON70680 is then cut with SnaBly and ligated with a gene fusion of the delta-9 humpback desaturase gene (SEQ ID NO: 41) in sense orientation regulated by the 7S promoter. The expression constructs pMON70680 and pMON70681 are used for the tranfromation of soybean using Agrobacterium methods. 2D Combination Constructs Expression constructs are made by containing several permutations of a first set of DNA sequences. The first set of DNA sequences are any of those described, or illustrated in Figures 5A-5D and 6A-6C, or any other set of DNA sequences containing various combinations of coding regions or non-coding FAD2, FAD3 and FATB sense or antisense, antisense sense so that they are capable of the formation of dsRNA constructs, sense co-deletion constructs, antisense constructs, or various combinations of the foregoing. Figures 5A-5C represent several first sets of DNA sequences that are capable of expressing sense antisense or co-deletion constructs in accordance with the present invention, the non-coding sequences that are described in the following Tables 1 and 2. The non-coding sequences can be single sequences, combinations of sequences (for example, the 5'UTR linked to the 3'UTR), or any combination of the above. To express a sense co-deleted construct, all non-coding sequences are sense sequences, and to express an antisense construct, all non-coding sequences are antisense sequences. Figure 5D depicts a first set of DNA sequences that are capable of expressing sense and antisense constructs in accordance with the present invention. Figures 6A-6C represent several first sets of DNA sequences that are capable of expressing dsRNA constructs according to the present invention, the non-coding sequences that are described in the following Tables 1 and 2. The first set of DNA sequences depicted in Figures 6A-6C comprise pairs of related sense and antisense sequences, arranged such that, for example, the RNA expressed by the first sense sequence is capable of forming a double-stranded RNA with the antisense RNA expressed by the first antisense sequence. For example, referring to Figure 6A and Illustrative Combination No. 1 (from Table 1), the first set of DNA sequences comprises a sense FAD2-1 sequence, a sense FAD3-1 sequence, an antisense and FAD2-1 sequence. an antisense FAD3-1 sequence. Both antisense sequences correspond to the sense sequences so that the expression products of the first set of DNA sequences are capable of forming a double-stranded RNA with one another. The sense sequences can be separated from the antisense sequences by a spacer sequence, preferably one that promotes the formation of a dsRNA molecule. Examples of such spacer sequences include those outlined in Wesley et al., Supra, and Hamilton et al., Plant J., 75: 737-746 (1988). The promoter is any functional promoter in a plant, or any plant promoter. Non-limiting examples of suitable promoters are described in part D of the detailed description. The first set of DNA sequences is inserted into an expression construct in sense or anti-sense orientation using a variety of DNA manipulation techniques. If convenient restriction sites are present in the DNA sequences, are inserted into the expression construct by digestion with restriction endonucleases and ligation in the construct that has been digested at one or more of the available cloning sites. If convenient restriction sites are not available in the DNA sequences, the construct DNA or sequences of DNA are modified in a variety of ways to facilitate the DNA sequences in the construct. Examples of methods for modifying the DNA are included by PCR, synthetic linker or adapter ligation, site-directed mutagenesis in vitro, filling or retro-cutting of the pendant 5 'or 3' ends and the like. These and other DNA manipulation methods are well known to those of ordinary skill in the art. pMON97552 contains a 7Sa 'soybean promoter operably linked to an intron 1 FAD2-1 A of soybean seed (SEQ ID NO: 1), which is reduced by 140 contiguous nucleotides of the 3' end, operably linked to 42 contiguous nucleotides from a FATB-1 to 5'UTR, followed by a CTP coding region FATB-1 a, operably linked to 70 nucleotides of intron 4 FAD3-1 A operably linked to a CTP coding region FATB-1 a in the anti-orientation - felt followed by 42 contiguous nucleotides of a FATB-1 at 5'UTR in the antisense orientation, followed by a soybean FAD2-1 A intron 1 (SEQ ID NO: 1), which is reduced by 140 contiguous nucleotides of the 3 'end and in the antisense orientation, operably linked to a 3' H6 polyadenylation segment with a CP4 EPSPS gene operably linked to an EFMV promoter and an E9 3 'end sequence. Pea Rubisco, all of which are flanked by a RB and a LB. The resulting gene expression construct is used for the transformation using methods as described herein. pMON93758 contains a 7Sa 'soybean promoter operably linked to an intron 1 FAD2-1 A of soybean (SEQ ID.

NO: 1), which is reduced by 160 contiguous nucleotides of the 5 'end and ligated to a FATB-1 to 3'UTR followed by a FATB-1 to 5'UTR operably linked to 70 nucleotides of intron 4 linked FAD3-1A operably at FATB-1 at 5'UTR in the antisense orientation, followed by an intron 1 FAD2-1 A of soybean (SEQ ID NO: 1), which is reduced by 160 contiguous nucleotides of the 5 'end and in the orientation antisense, operably linked to a 3 'H6 polyadenylation segment with a CP4 EPSPS gene operably linked to an EFMV promoter and a E9 3' end sequence. Pea Rubisco flanked by RB and LB on the same DNA molecule. The resulting gene expression construct is used for the transformation using methods as described herein. pMON97553 contains a soybean 7Sa 'promoter operably linked to a soybean FAD2-1 A intron 1 (SEQ ID NO: 1), which is reduced by 200 contiguous nucleotides from the 3' end and ligated to 42 contiguous nucleotides from a FATB-1 to 5'UTR followed by a CTP coding region FATB-1 operably linked to 70 nucleotides of intron 4 FAD3-1 A operably linked to a CTP coding region FATB-1 a in the antisense orientation followed by 42 contiguous nucleotides of a FATB-1 to 5'UTR in the antisense orientation, followed by a soybean FAD2-1 A intron 1 (SEQ ID NO: 1), which is reduced by 200 contiguous nucleotides of the 3 'end and in the antisense orientation, operably linked to a 3 'H6 polyadenylation segment with a CP4 EPSPS gene operably linked to an EFMV promoter and a termination E9 3 'Rubisco of pea flanked by RB and LB in the same DNA molecule. The resulting gene expression construct is used for the transformation using methods as described herein. pMON93770 contains a 7S soybean promoter operably linked to a soybean FAD2-1A intron 1 (SEQ ID NO: 1), which is reduced by 240 contiguous nucleotides from the 3 'end and ligated to a FATB-1 at 3'UTR and followed by a FATB-1 to 5'UTR operably linked to 70 nucleotides of intron 4 FAD3-1A operably linked to FATB-1 at 5'UTR in the antisense orientation, followed by FATB-1 to 3'UTR in the antisense orientation, followed by an intron 1 FAD2-1 A of soybean seed (SEQ ID NO: 1), which is reduced by contiguous 240 nucleotides of the 3 'end and in antisense orientation, operably linked to a 3 'H6 polyadenylation segment with a CP4 EPSPS gene operably linked to an EFMV promoter and a 3' E9 termination sequence Pea Rubisco flanked by RB and LB on the same DNA molecule. The resulting gene expression construct is used for the transformation using methods as described herein. pMON93759 contains a soybean 7Sa 'promoter operably linked to a soybean FAD2-1 A intron 1 (SEQ ID NO: 1), which is reduced by 240 contiguous nucleotides from the 5' end and ligated to a FATB- 1 to 3'UTR followed by a FATB-1 to 5'UTR operably linked to 70 nucleotides of intron 4 FAD3-A operably linked to a FATB-1 to 5'UTR in the antisense orientation followed by a FATB-1 to 3'UTR in the antisense orientation, followed by a soybean FAD2-1 A intron 1 (SEQ ID NO: 1), which is reduced by 240 contiguous nucleotides of the 5 'end and in the antisense orientation, operably linked to a polyadenylation segment H6 3 'with a CP4 EPSPS gene operably linked to an EFMV promoter and a E9 3' termination sequence Pea Rubisco flanked by RB and LB in the same DNA molecule. The resulting gene expression construct is used for the transformation using methods as described herein. pMON97554 contains a soybean 7Sa 'promoter operably linked to an intron soybean FAD2-1 A (SEQ ID NO: 1), which is reduced by 260 contiguous nucleotides from the 3' end and ligated to 42 contiguous nucleotides from a FATB-1 to a 5'UTR followed by a CTP coding region FATB-1 a, operably linked to 70 nucleotides of intron 4 FAD3-1 A, operably linked to a CTP coding region FATB-1 a in antisense orientation followed by 42 contiguous nucleotides of a FATB-1 at 5'UTR in the antisense orientation, followed by a soybean FAD2-1 A intron 1 (SEQ ID NO: 1), which is reduced by 260 contiguous nucleotides of the 3-terminal and in the antisense orientation, operably linked to a 3 H6 polyadenylation segment with a CP4 EPSPS gene operably linked to an EFMV promoter and an E9 3 'termination sequence. Pea Rubisco flanked by RB and LB on the same DNA molecule . The resulting gene expression construct is used for the transformation using methods as described herein. pMON93771 contains a soybean 7Sa 'promoter operably linked to a soybean FAD2-1 A intron 1 (SEQ ID NO: 1), which is reduced by 300 contiguous nucleotides from the 3' end and ligated to a FATEM at 3'UTR followed by a FATB-1 at 5'UTR, operably linked to 70 nucleotides of intron 4 FAD3-1 A operably linked to a FATB-1 at 5'UTR in the antisense orientation followed by a FATB-1 at 3 ' UTR in the antisense orientation, followed by a soybean FAD2-1 A intron 1 (SEQ ID NO: 1), which is reduced by 300 contiguous nucleotides of the 3 'end and in the antisense orientation, operably linked to a segment of polyadenylation H6 3 'with a CP4 EPSPS gene operably linked to an EFMV promoter and an E9 3' termination sequence Pea Rubisco flanked by RB and LB on the same DNA molecule. The resulting gene expression construct is used for the transformation using methods as described herein. pMON97555 contains a soybean 7Sa 'promoter operably linked to an soybean FAD2-1 A intron 1 (SEQ ID NO: 1), which is reduced by 320 contiguous nucleotides from the 3' end and ligated to 42 contiguous nucleotides from a FATB-1 to 5'UTR followed by a CTP-coding region FATB-1 operably linked to 70 nucleotides of intron 4 FAD3-1A, operably linked to a CTP coding region FATB-1 a in the antisense orientation followed by 42 contiguous nucleotides from a FATB-1 to 5'UTR in the antisense orientation, followed by an intron 1 FAD2-1 A of soybean seed (SEQ ID NO: 1), which is reduced by 320 nucleotides contiguous of the 3 'end and in the antisense orientation, operably linked to a 3' H6 polyadenylation segment with a CP4 EPSPS gene operably linked to an EFMV promoter and a E9 3 'termination sequence. Pea Rubisco flanked by RB and LB in the same DNA molecule. The resulting gene expression construct is used for the transformation using methods as described herein. pMON93760 contains a soybean 7Sa 'promoter operably linked to a soybean FAD2-1 A intron 1 (SEQ ID NO: 1), which is reduced by 320 contiguous nucleotides of the 5' end and ligated to a FATB- 1 to 3'UTR followed by a FATB-1 at 5'UTR, operably linked to 70 nucleotides of intron 4 FAD3-1 A operably linked to a FATB-1 at 5'UTR in the antisense orientation followed by a FATB-1 at 3'UTR in the antisense orientation followed by an intron 1 FAD2-1 A of soybean seed (SEQ ID NO: 1), which is reduced by 320 contiguous nucleotides of the 5 'end and in the antisense orientation, operably linked to a 3' H6 polyadenylation segment with a CP4 EPSPS gene operably linked to an EFMV promoter and an E9 3 'termination sequence. flanked by RB and LB in the same DNA molecule. The resulting gene expression construct is used for the transformation using methods as described herein. pMON93772 contains a soybean 7Sa 'promoter operably linked to an intron soybean FAD2-1 A (SEQ ID NO: 1), which is reduced by 360 contiguous nucleotides of the 3' end and ligated to FATB-1 at 3'UTR and followed by FATB-1 at 5'UTR operably linked to 70 nucleotides of intron 4 FAD3-1 A, operably linked to a FATB-1 at 5'UTR in the antisense orientation followed by a few FATB- 1 to 3 'UTR in the antisense orientation, followed by an intron 1 FAD2-1 A of soybean seed (SEQ ID NO: 1), which is reduced by 360 contiguous nucleotides of the 3' end and in the antisense orientation, operably linked to a polyadenylation segment H6 3 'with a CP4 EPSPS gene operably linked to an EFMV promoter and an E9 3' termination sequence. Pea Rubisco flanked by RB and LB in the same DNA molecule. The resulting gene expression construct is used for the transformation using methods as described herein. pMON97556 contains a soybean 7Sa 'promoter operably linked to a soybean FAD2-1 A intron 1 (SEQ ID NO: 1), which is reduced by 380 contiguous nucleotides from the 3' end and ligated to 42 contiguous nucleotides from a FATB-1 to 5'UTR followed by a CTP coding region FATB-1 operably linked to 70 nucleotides of intron 4 FAD3-1 A, operably linked to a CTP coding region FATB-1 a in the antisense orientation followed for 42 contiguous nucleotides of a FATB-1 to 5'UTR in the antisense orientation, operably linked to a soybean FAD2-1 A intron 1 (SEQ ID NO: 1), which is reduced by 380 contiguous nucleotides of the 3-terminal and in the antisense orientation, operably linked to a polyadenylation segment H6 3 'with a CP4 EPSPS gene operably linked to an EFMV promoter and a sequence of termination E9 3 'Rubisco of pea flanked by RB and LB in the same DNA molecule. The resulting gene expression construct is used for the transformation using methods as described herein. pMON93764 contains a soybean 7Sa promoter operably linked to an intron soybean FAD2-1 A (SEQ ID NO: 1), which is reduced by 400 contiguous nucleotides of the 3 'end and ligated to a coding region CTP FATB-1 a followed by a CTP coding region FATB-2a operably linked to 70 nucleotides of intron 4 FAD3-1 A, operably linked to a CTP coding region FATB-2a in the antisense orientation followed by a CTP coding region FATB-1 a in the antisense orientation, followed by an intron 1 FAD2-1 A of soybean seed (SEQ ID NO: 1), which is reduced by 400 contiguous nucleotides of the 3 'end and in the antisense orientation, operably linked to a CTP coding region FATB-2a in the antisense orientation followed by 42 contiguous nucleotides of a 5'UTR FATB-2a in the antianthisense orientation operably linked to a 3 'H6 polyadenylation segment with a linked CP4 EPSPS gene opera pea to an EFMV promoter and an E9 3 'termination sequence. Pea Rubisco flanked by RB and LB in the same DNA molecule. The resulting gene expression construct is used for the transformation using methods as described herein.

TABLE 1 Combines coding or non-coding sequences (sense or antisense) - First First Third Third Fourth illustrative 1 FAD2-1A or B FAD3-1A or B or C 2 FAD3-1A or B FAD2-1A or B oC 3 FAD2-1A or B FAD3-1A or B or FAD3-1A C different or sequence B or C 4 FAD2-1A or B FAD3-1A or B or FATB-1 C 5 FAD2-1 A or B FATB-1 FAD3-1A or B or C 6 FAD3-1A or B FAD2-1A or B FATB-1 oC 7 FAD3-1A or B FATB-1 FAD2-1A or B oC 8 FATB-1 FAD3-1A or B or FAD2-1A or BC 9 FATB-1 FAD2- 1A or B FAD3-1A or B or C 10 FAD2-1A or B FAD3-1A or B or FAD3-1A FATB-1 C different or sequence B or C 11 FAD3-1A or B FAD2-1A or B FAD3-1A FATB -1 oC different or sequence B or C 12 FAD3-1A or B FAD3-1A FAD2-1A or B FATB-1 oC different or sequence B or C 13 FAD2-1A or B FAD3-1A or B or FATB-1 FAD3- 1A different C or sequence B or C 14 FAD3-1A or B FAD2-1A or B FATB-1 FAD3-1A oC different or sequence B or C FAD3-1A or B FAD3-1A FATB-1 FAD2-1A or B oC different or sequence B or C FAD2-1A or B FATB-1 FAD3-1A or B or FAD3-1A C different or sequence B or C FAD3-1A or B FATB-1 FAD2-1A or B FAD3-1A oC different or sequence B or C FAD3- A or B FATB-1 FAD3-1A FAD2-1A or B oC different or sequence B or C FATB-1 FAD2-1A or B FAD3-1A or B or FAD3-1A C different or sequence B or C FATB-1 FAD3-1A or B FAD2-1A or B FAD3-1A oC different or sequence B or C FATB-1 FAD3-1A or B FAD3-1A FAD2-1A or B oC different or sequence B or C FAD2-1 A or B FAD3-1A or B FATB-2 oC FAD2-1A or B FATB-2 FAD3-1A or B or C FAD3-1A or B FAD2-1A or B FATB-2 oC FAD3-1A or B FATB-2 FAD2-1A or B oC FATB-2 FAD3-1A or B FAD2-1A or B oC FATB-2 FAD2-1 A or B FAD3-1A or B or C FAD2-1A or B FAD3-1A or B FAD3-1A FATB-2 oC different or sequence B or C FAD3-1A or B FAD2-1 A or B FAD3-1A FATB-2 oC different or sequence B or C FAD3-1A or B FAD3-1A FAD2-1A or B FATB-2 oC different or sequence B or C FAD2-1 A or B FAD3-1A or B or FATB-2 FAD3-1A C different or sequence B or C FAD3-1A or B FAD2-1A or B FATB-2 FAD3-1A oC different or sequence B or C FAD3-1A or B FAD3-1A FATB-2 FAD2-1A or B oC different or sequence B or C FAD2-1 A or B FATB-2 FAD3-1A or B or FAD3-1A C different or sequence B or C FAD3-1A or B FATB-2 FAD2-1A or B FAD3-1A oC different or sequence B or C FAD3-1A or B FATB-2 FAD3-1A FAD2-1A or B oC different or sequence B or C FATB-2 FAD2-1A or B FAD3-1A or B or FAD3-1A C different or sequence B or C FATB-2 FAD3-1A or B or FAD2-1 A or B FAD3-1A C different or sequence B or C FATB-2 FAD3-1A or B or FAD3-1A FAD2-1A or B C different or sequence B or C TABLE 2 EXAMPLE 3 Combination constructions In Figures 7-15, promoters are indicated by arrows, coding sequences (both coding or non-coding) are indicated by pentagons whose point in the direction of transcription, the sense sequences are labeled in normal text, and antisense sequences are tag in inverted text. The abbreviations used in these figures include: promoter 7Sa = 7Sa; promoter 7Sa '= 7Sa'; Brassica napin promoter; FMV = an FMV promoter; ARC = arcelin promoter; RBC EO 3 '= termination signal E9 Rubisco; We 3 '= sign of ending us; TML 3 '= termination signal tml; napin 3 '= napin termination signal; '3 (in the same box as FAD or FAT) = 3' UTR; 5 '(in the same box as FAD or FAT); 5'UTR; Cr = Cuphea pulcherrima; Gm = glycerin max; Re = Ricimus communis; FAB2 = a FAB2 allele of a delta 9 stearoyl desaturase gene; and Intr or Int = intron. 3A. DsRNA constructs Figures 7-9 represent nucleic acid molecules of the present invention in which the first sets of DNA sequences are capable of expressing dsRNA constructs. The first set of DNA sequences depicted in Figures 7-9 comprise pairs of related sense and antisense sequences, arranged as, for example, the RNA expressed by the first sense sequence is capable of forming a double-stranded RNA with the RNA antisense expressed by the first antisense sequence. The sense sequences may be adjacent to the antisense sequences, or separated from the antisense sequences by a spacer sequence, as shown in Figure 9. The second set of DNA sequences comprises coding sequences, each of which is a sequence of DNA encoding a sequence that when expressed is capable of increasing one or both of the protein and transcript encoded by a gene selected from the group consisting of KAS I, KAS IV, delta-9 desaturase, and CP4 EPSPS. Each coding sequence is associated with a promoter, which may be any functional promoter in a plant, or any plant promoter, and may be an FMV promoter, a napin promoter, a 7S promoter (either 7Sa or 7Sa '), a promoter of arcelin, a delta-9 desaturase promoter, or a FAD2-1 A promoter. Referring now to Figure 7, soybean intron 1 FAD2-1 sequences (SEQ ID NO: 1 or 2), FAD3-1 A 3'UTR (SEO ID NO: 16), and FATB-1 3'UTR (SEQ ID NO: 36) are amplified via PCR to result in PCR products that include restriction sites redesigned at both ends. The PCR products are cloned directly, in sense and antisense orientations, separated by a spliceable soybean FAD3-1 A intron (SEQ ID NO: 1 1), in a vector containing the seed 7Sa 'promoter. of soybeans and a tml 3 'terminator sequence, in the form of Xho sites designed at the 5' ends of the PCR primers. The vector is then cut with Noñ and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and a 3 'E9 termination sequence. Rubisco of pea. Vectors containing a KAS IV C. pulcherríma gene (SEQ ID NO: 39) regulated by a Brassica napin promoter and a 3 'napin termination sequence Brassica; and a delta-9 R. communis desaturase gene (FAB2) (SEQ ID NO: 40) regulated by an FAD2 promoter of soybean seed and a 3 'end sequence, is cut with appropriate restriction enzymes, and ligated into pMON41 164. The resulting gene expression construct, pMON68539, is depicted in Figure 7 and used for transformation using methods as described here. Sequences of intron 1 FAD2-1 of soybean seed (SEQ ID NO: 1 or 2), intron 4 FAD3-1 A (SEQ ID NO: 10), and intron II FATB-1 (SEQ ID NO: 30) are amplified via PCR to result in PCR products that include restriction sites redesigned at both ends . The PCR products are cloned directly, in sense and antisense orientations, separated by a spliceable soybean FAD3-1 A intron (SEQ ID NO: 1 1), in a vector containing the seed 7Sa 'promoter. of soybean and a sequence of completion ÍAT? / 3 ', in the manner of Xho sites \ designed at the 5' ends of the PCR primers. The vector is then cut with Noft and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and a 3 'E9 termination sequence. Pea Rubisco. The resulting gene expression construct, pMON68540, is depicted in Figure 7 and used for transformation using methods as described herein. Sequences of intron 1 FAD2-1 of soybean (SEQ ID NO: 1 or 2), intron 4 FAD3-1A (SEQ ID NO: 10), and intron II FATB-1 (SEQ ID NO: 30) are amplified via PCR to result in PCR products that include restriction sites redesigned at both ends. PCR products are cloned directly, in sense and antisense orientations, separated by an intron 5 FAD3-1 A of spliceable soybean (SEQ ID NO: 1 1), in a vector containing the soybean 7Sa 'promoter and a fm / 3' termination sequence, as Xho sites \ designed at the 5 'ends of the PCR primers. The vector is then cut with Noñ and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and a 3 'E9 termination sequence. Rubisco of pea. A vector containing a KAS IV C. pulcherrima gene (SEQ ID NO: 39) regulated by a Napin Brassica promoter and a 3 'Napin termination sequence Brassica is cut with appropriate restriction enzymes, and ligated into pMON41 164. The The resulting gene expression construct, pMON68544, is depicted in Figure 7 and used for the transformation using methods as described herein. Sequences of intron 1 FAD2-1 of soybean (SEQ ID NO: 1 or 2), intron 4 FAD3-1A (SEQ ID NO: 10), intron II FATB-1 (SEQ ID NO: 30), and intron 4 FAD3-1 B (SEQ ID NO: 24) are amplified via PCR to result in PCR products that include restriction sites redesigned at both ends. The PCR products are cloned directly, in sense and antisense orientations, separated by a spliceable soybean FAD3-1 A intron (SEQ ID NO: 1), in a vector containing the seed 7Sa 'promoter. soybean and a tml 3 'termination sequence, in the form of Xho sites designed at the 5' ends of the PCR primers. The vector is then cut with Noñ and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and a sequence Termination E9 3 'Pea Rubisco. The resulting gene expression construct, pMON68546, is depicted in Figure 7 and used for transformation using methods as described herein. Referring now to Figure 8, intron sequences 1 FAD2-1 of soybean (SEQ ID NO: 1 or 2), FAD3-1 A 3'UTR (SEQ ID NO: 16) and FATB-1 3'UTR (SEQ ID NO: 36) are amplified via PCR to result in PCR products that include restriction sites redesigned at both ends. The PCR products are cloned directly, in sense and antisense orientations, separated by a spliceable soybean FAD3-1 A intron (SEQ ID NO: 1 1), in a vector containing the seed 7Sa 'promoter. of soybeans and a tml 3 'termination sequence, as Xho sites designed at the 5 'ends of the PCR primers. The vector is then cut with Noti and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and a 3 'E9 termination sequence. Pea Rubisco. The resulting gene expression construct, pMON68536, is depicted in Figure 8 and used for transformation using methods as described herein. Sequences of intron 1 FAD2-1 of soybean (SEQ ID NO: 1 or 2), FAD3-1 A 3'UTR (SEQ ID NO: 16) and FATB-1 3'UTR (SEQ ID NO: 36) are amplify via PCR to result in PCR products that include restriction sites redesigned at both ends. The PCR products are cloned directly, in sense and antisense orientations, separated by a spherical soybean intron FAD3-1 A (SEQ ID NO: 1 1), in a vector containing the soybean 7Sa 'promoter and a rm / 3' termination sequence, in the form of Xho sites designed at the 5 'ends of the PCR primers. A vector containing a delta-9 R. communis desaturase gene (FAB2) (SEQ ID NO: 40) regulated by a soybean FAD2 promoter and a 3 'nos termination sequence, is cut with appropriate restriction enzymes, and they are ligated just upstream of the tml 3 'termination sequence. The vector is then cut with Noñ and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and a 3 'E9 termination sequence. Rubisco of pea. The resulting gene expression construct, pMON68537, is depicted in Figure 8 and used for transformation using methods as described herein. Sequences of intron 1 FAD2-1 of soybean (SEQ ID NO: 1 or 2), FAD3-1 A 3'UTR (SEQ ID NO: 16), and FATB-1 3'UTR (SEQ ID NO: 36) they are amplified via PCR to result in PCR products that include restriction sites redesigned at both ends. The PCR products are cloned directly, in sense and antisense orientations, separated by a splicing soybean intron FAD3-1 A (SEQ ID NO: 1 1), in a vector containing the 7Sa seed promoter. soybean and a rm / 3 'termination sequence, in the form of Xho sites designed at the 5' ends of the PCR primers. The vector is then cut with Noñ and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and a 3 'E9 termination sequence. Rubisco of pea. A vector containing a KAS IV C. pulcherrima gene (SEQ ID NO: 39) regulated by a Brassica napin promoter and a 3 'napin termination sequence Brassica is cut with appropriate restriction enzymes, and ligated into pMON41 164. The construct The expression of the resulting gene, pMON68538, is represented in FIG. 8 and used for the transformation using methods as described herein. Referring now to Figure 9, FAD2-1 3'UTR sequences of soybean (SEQ ID NO: 5), FATB-1 3'UTR (SEQ ID NO: 36), FAD3-1 A 3'UTR ( SEQ ID NO: 16) and FAD3-1 B 3'UTR (SEQ ID NO: 26) are amplified via PCR to result in PCR products that include restriction sites redesigned at both ends. The PCR products are cloned directly, in sense and antisense orientations, separated by a spliceable soybean FAD3-1 A intron (SEQ ID NO: 1 1), in a vector containing the seed 7Sa 'promoter. of soybeans and a tml 3 'terminator sequence, in the form of Xho sites designed at the 5' ends of the PCR primers. The vector is then cut with Noft and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and a 3 'E9 termination sequence. Pea Rubisco. The resulting gene expression construct, pMON80622, is depicted in Figure 9 and used for transformation using methods as described herein. Sequences of FAD2-1 3'UTR of soybean (SEQ ID NO: 5), FATB-1 3'UTR (SEQ ID NO: 36), and FAD3-1 A 3'UTR (SEQ ID NO: 16) are amplify via PCR to result in PCR products that include restriction sites redesigned at both ends. The PCR products are cloned directly, in sense and antisense orientations, separated by a spliceable soybean FAD3-1 A intron (SEQ ID NO: 1 1), in a vector containing the seed 7Sa 'promoter. of soybeans and a tml 3 'terminator sequence, in the form of Xho sites designed at the 5' ends of the PCR primers. The vector is then cut with Noti and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and a 3 'E9 termination sequence. Rubisco of pea. The resulting gene expression construct, pMON80623, is depicted in Figure 9 and used for transformation using methods as described herein. Sequences of FAD2-1 5'UTR-3'UTR of soybean seed (SEQ ID NOs: 6 and 5, linked together), FATB-1 5'UTR-3'UTR (SEQ ID NOs: 37 and 36, linked together), FAD3-1A 3'UTR (SEQ ID NO: 16) and FAD3-1 B 5'UTR- 3'UTR (SEQ ID NOs: 27 and 26, ligated together) are amplified via PCR to result in PCR products that include restriction sites redesigned at both ends. The PCR products are cloned directly, in sense and antisense orientations, into a vector containing the soybean 7S 'promoter and a 3' tml termination sequence, as Xho sites designed at the 5 'ends of the primers PCR The vector is then cut with Noti and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and a 3 'E9 terminator sequence.

Pea Rubisco The resulting gene expression construct, 05, is depicted in Figure 9 and used for transformation using methods as described herein. Sequences of FAD2-1 5'UTR-3'UTR of soybean (SEQ ID NOs: 6 and 5, linked together), FATB-1 5'UTR-3'UTR (SEQ ID NOs: 37 and 36, linked together ) and FAD3-1 A 3'UTR (SEQ ID NO: 16) are amplified via PCR to result in PCR products that include restriction sites redesigned at both ends. The PCR products are cloned directly, in sense and antisense orientations, into a vector containing the soybean 7Soc 'promoter and a tml 3' terminator sequence, in the form of Xho sites designed at the 5 'ends of the primers PCR The vector is then cut with Noñ and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and a 3 'E9 termination sequence. Rubisco of pea. A vector containing a KAS IV C. pulcherrima gene (SEQ ID NO: 39) regulated by a Napin Brassica promoter and a 3 'napin termination sequence Brassica is cut with appropriate restriction enzymes, and ligated into pMON41 164. The The resulting gene expression construct, 06, is depicted in Figure 9 and used for transformation using methods as described herein. 3B. Conscious Co-Deletion Constructs Figures 10-13 and 19A-20C represent nucleic acid molecules of the present invention wherein the first set of sequences of DNA are able to express constructs of sense co-suppression. The second set of DNA sequences comprises coding sequences, each of which is a DNA sequence encoding a sequence that when expressed is capable of increasing one or both of the protein and transcript encoded by a gene selected from the group It consists of KAS I, KAS IV, delta-9 desaturase, and CP4 EPSPS. Each coding sequence is associated with a promoter, which is any functional promoter in a plant, or any plant promoter, and can be an FMV promoter, a napin promoter, a 7S promoter (7Sa or 7Sa), an arcelin promoter, a promoter delta-9 desaturase, or a FAD2-1A promoter. Now with reference to Figure 10, intron sequences 1 FAD2-1 of soybean (SEQ ID NO: 1 or 2), intron 4 FAD3-1 C (SEQ ID NO: 14), intron II FATB-1 (SEQ ID NO: 30), intron 4 FAD3-1 A (SEQ ID NO: 10), and intron 4 FAD3-1 B (SEQ ID NO: 24) are amplified via PCR to result in PCR products that include re-designed restriction sites at both ends. The PCR products are cloned directly, in sense orientation, into a vector containing the 7Sa 'promoter of soybean and termination sequence E9 3' Rubisco of pea, in the form of Xhol sites designed on the 5 'ends of the PCR primers . The vector is then cut with Notl and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and a 3 'E9 termination sequence. Rubisco of pea. The resulting gene expression construct, pMON68522, is depicted in Figure 10 and used for transformation using methods such as those described here. Intron 1 FAD2-1 sequences of soybean (SEQ ID NO: 1 or 2), intron 4 FAD3-1A (SEQ ID NO: 10), intron 4 FAD3-1 B (SEQ ID NO: 24), and intron II FATB-1 (SEQ ID NO: 30) are amplified via PCR to result in PCR products that include re-designed restriction sites at both ends. The PCR products are cloned directly, in sense orientation, into a vector containing the soybean 7Sa 'promoter and a 3' tml termination sequence, in the form of Xhol sites designed on the 5 'ends of the PCR primers. The vector is then cut with Notl and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and a 3 'E9 termination sequence. Rubisco of pea. Vectors containing a C. pulcherrima KAS IV gene (SEQ ID NO: 39) regulated by a Brassica napin promoter and a 3 'napin Brassica termination sequence, and a R. communis delta 9 desaturase gene (FAB2) (SEQ ID NO: 40) regulated by a soybean FAD2 promoter and a 3 'nos termination sequence, are cut with appropriate restriction enzymes, and ligated into pMON41 164. The resulting gene expression construct, pMON80614, is depicted in Figure 10. and it is used for the transformation using methods such as those described herein. Intron 1 FAD2-1 sequences of soybean (SEQ ID NO: 1 or 2), FAD3-1 A 3'UTR (SEQ ID NO: 16) and FATB-1 3 'UTR (SEQ ID NO: 36) are amplified via PCR to result in PCR products that include re-designed restriction sites in both extremes. The PCR products are cloned directly, in sense orientation, in a vector containing the soybean 7Sa 'promoter and a 3' tml termination sequence, in the form of Xhol sites designed on the 5 'ends of the PCR primers. The vector is then cut with Notl and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and a 3 'E9 termination sequence. Rubisco of pea. The resulting gene expression construct, pMON68531, is depicted in Figure 10 and used for transformation using methods such as those described herein. Intron sequences 1 FAD2-1 from soybean (SEQ ID NO: 1 or 2), FAD3-1 A 3'UTR (SEQ ID NO: 16) and FATB-1 3 'UTR (SEQ ID NO: 36) are amplified via PCR to result in PCR products that include re-designed restriction sites at both ends. The PCR products are cloned directly, in sense orientation, into a vector containing the soybean 7Sa 'promoter and a 3' tml termination sequence, in the form of Xhol sites designed on the 5 'ends of the PCR primers. The vector is then cut with Notl and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and a 3 'E9 termination sequence. Rubisco of pea. Vectors containing a C. pulcherrima KAS IV gene (SEQ ID NO: 39) regulated by a Brassica napin promoter and a 3 'napin Brassica termination sequence, and a R. communis delta 9 desaturase gene (FAB2) (SEQ ID NO: 40) regulated by a soybean FAD2 promoter and a 3 'end sequence, are cut with appropriate restriction enzymes, and ligated into pMON41 164. The The resulting gene expression, pMON68534, is depicted in Figure 10 and used for transformation using methods such as those described herein. Intron sequences 1 FAD2-1 from soybean (SEQ ID NO: 1 or 2), FAD3-1 A 3'UTR (SEQ ID NO: 16) and FATB-1 3 'UTR (SEQ ID NO: 36) are amplified via PCR to result in PCR products that include re-designed restriction sites at both ends. The PCR products are cloned directly, in sense orientation, into a vector containing the soybean 7Sa 'promoter and a 3' tml termination sequence, in the form of Xhol sites designed on the 5 'ends of the PCR primers. The vector is then cut with Notl and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and a 3 'E9 termination sequence. Rubisco of pea. A vector containing a delta 9 desaturase gene R. communis (FAB2) (SEQ ID NO: 40) regulated by a soybean FAD2 promoter and a 3 'nos termination sequence, is cut with appropriate restriction enzymes, and liga in pMON41 164. The resulting gene expression construct, pMON68535, is depicted in Figure 10 and used for transformation using methods such as those described herein. Now with reference to Fig. 11, sequences FAD2-1 3'UTR of soybean (SEQ ID NO: 5), FAD3-1 A 3'UTR (SEQ ID NO: 16), and FATB-1 3 'UTR (SEQ ID NO: 36) are amplified via PCR to result in PCR products that include re-designed restriction sites at both ends. The PCR products are cloned directly, in sense orientation, into a vector containing the soybean 7Sa 'promoter and a Termination sequence tml 3 ', in the form of Xhol sites designed on the 5' ends of the PCR primers. The vector is then cut with Notl and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and a 3 'E9 termination sequence. Rubisco of pea. The resulting gene expression construct, pMON80605, is depicted in Figure 11 and used for transformation using methods such as those described herein. Sequences FAD2-1 3'UTR of soybean seed (SEQ ID NO: 5), FAD3-1 A 3'UTR (SEQ ID NO: 16), and FATB-1 3 'UTR (SEQ ID NO: 36) are amplified via PCR to result in PCR products including re-designed restriction sites at both ends . The PCR products are cloned directly, in sense orientation, into a vector containing the soybean 7Sa 'promoter and a 3' tml termination sequence, in the form of Xhol sites designed on the 5 'ends of the PCR primers. The vector is then cut with Notl and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and a 3 'E9 termination sequence. Rubisco of pea. A vector containing a C. pulcherrima KAS IV gene (SEQ ID NO: 39) regulated by a Napin Brassica promoter and a 3 'termination sequence Brassica napin is cut with appropriate restriction enzymes, and ligated into pMON4 164. The construct The resulting gene expression, pMON80606, is depicted in Figure 11 and used for transformation using methods such as those described herein. Sequences FAD2-1 3'UTR of soybean seed (SEQ ID NO: 5), FAD3-1 A 3'UTR (SEQ ID NO: 16), and FATB-1 3 'UTR (SEQ ID NO: 36) are amplified via PCR to result in PCR products that include re-engineered restriction sites at both ends. The PCR products are cloned directly, in sense orientation, into a vector containing the soybean 7Sa 'promoter and a 3' tml termination sequence, in the form of Xhol sites designed on the 5 'ends of the PCR primers. The vector is then cut with Notl and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and a 3 'E9 termination sequence. Rubisco of pea. A vector containing a delta 9 desaturase gene R. communis (FAB2) (SEQ ID NO: 40) regulated by a soybean FAD2 promoter and a 3 'nos termination sequence, is cut with appropriate restriction enzymes, and liga in pMON41 164. The resulting gene expression construct, pMON80607, is depicted in Figure 11 and used for transformation using methods such as those described herein. Sequences FAD2-1 3'UTR of soybean seed (SEQ ID NO: 5), FAD3-1 A 3'UTR (SEQ ID NO: 16), and FATB-1 3 'UTR (SEQ ID NO: 36) are amplified via PCR to result in PCR products that include re-engineered restriction sites at both ends. The PCR products are cloned directly, in sense orientation, into a vector containing the soybean 7Sa 'promoter and a 3' tml termination sequence, in the form of Xhol sites designed on the 5 'ends of the PCR primers. The vector is then cut with Notl and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and a sequence of Termination E9 3 'Pea Rubisco. Vectors containing a C. pulcherrima KAS IV gene (SEQ ID NO: 39) regulated by a Brassica napin promoter and a 3 'napin Brassica termination sequence, and a R. communis delta 9 desaturase gene (FAB2) (SEQ ID NO: 40) regulated by a soybean FAD2 promoter and a 3 'nos termination sequence, cut with appropriate restriction enzymes, and ligated into pMON41 164. The resulting gene expression construct, pMON80614, is depicted in Figure 1 1 and is used for transformation using methods such as those described herein. Now with reference to Figure 12, FAD2-1 3'UTR sequences of soybean (SEQ ID NO: 5), FATB-1 3 'UTR (SEQ ID NO: 36) and FAD3-1 A 3'UTR (SEQ. ID NO: 16) are amplified via PCR to result in PCR products that include re-designed restriction sites at both ends. The PCR products are cloned directly, in sense orientation, into a vector containing the soybean 7Sa promoter and a 3 'tml termination sequence, in the form of Xhol sites designed on the 5' ends of the PCR primers. The vector is then cut with Notl and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and a 3 'E9 termination sequence. Rubisco of pea. The resulting gene expression construct, pMON80629, is depicted in Figure 12 and used for transformation using methods such as those described herein. Intron 1 FAD2-1 sequences of soybean (SEQ ID NO: 1 or 2), intron 4 FAD3-1 A (SEQ ID NO: 10), intron II FATB-1 (SEQ ID NO: 30), and Intron 4 FAD3-1 A (SEQ ID NO: 10) are amplified via PCR to result in PCR products that include re-designed restriction sites at both ends. The PCR products are cloned directly, in sense orientation, in a vector containing the soybean 7S promoter and a 3 'tml terminator sequence, in the form of Xhol sites designed on the 5' ends of the PCR primers. The vector is then cut with Notl and ligated into pMON4 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and a 3 'E9 termination sequence. Rubisco of pea. The resulting gene expression construct, pMON81902, is depicted in Figure 12 and used for transformation using methods such as those described herein. FAD2-1 5'UTR-3'UTR sequences of soybean seeds (SEQ ID NOs: 6 and 5, linked together), FAD3-1 5'UTR-3'UTR (SEQ ID NOs: 17 and 16, linked together, or 27 and 26, linked together), and FATB-1 5'UTR-3'UTR (SEQ ID NOs: 37 and 36, linked together) are amplified via PCR to result in PCR products that include re-designed restriction sites at both ends. The FAD2-1 PCR product is cloned directly, in sense orientation, into a vector containing the soybean 7Sa 'promoter and a 3' tml termination sequence, in the form of Xhol sites designed on the 5 'ends of the primers PCR Similarly, the FAD3-1 PCR product is cloned directly, in sense orientation, into a vector containing the soybean 7Soc promoter and a 3 'tml terminator sequence, as Xhol sites designed on the 5' ends from the PCR primers. The FATB-1 PCR product is cloned directly, in sense orientation, into a vector containing the arcelin promoter and a 3 'tml terminator sequence, in the form of Xhol sites designed on the 5' ends of the PCR primers. These vectors are then cut with Notl and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and an E9 3 'Rubisco pea. The resulting gene expression construct, 01, is depicted in Figure 12 and used for transformation using methods such as those described herein. Sequences FAD2-1 5'UTR-3'UTR of soybean seed (SEQ ID NOs: 6 and 5, linked together), FAD3-1 5'UTR-3'UTR (SEQ ID NOs: 17 and 16, linked together, or 27 and 26, linked together), and FATB-1 5 ' UTR-3'UTR (SEQ ID NOs: 37 and 36, bound together) are amplified via PCR to result in PCR products that include re-designed restriction sites at both ends. The FAD2-1 PCR product is cloned directly, in sense orientation, into a vector containing the soybean 7Sa 'promoter and a 3' tml termination sequence, in the form of Xhol sites designed on the 5 'ends of the primers PCR Similarly, the FAD3-1 PCR product is cloned directly, in sense orientation, into a vector containing the soybean 7Sa promoter and a 3 'tml terminator sequence, as Xhol sites designed on the 5' ends of PCR primers. The FATB-1 PCR product is cloned directly, in sense orientation, into a vector containing the arcelin promoter and a Termination sequence tml 3 ', in the form of Xhol sites designed on the 5' ends of the PCR primers. These vectors are then cut with Notl and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and a 3 'E9 termination sequence. Rubisco of pea. A vector containing a C. pulcherrima KAS IV gene (SEQ ID NO: 39) regulated by a Napin Brassica promoter and a 3 'termination sequence napin Brassica is cut with appropriate restriction enzymes, and ligated into pMON41 164. The construct of The resulting gene expression, 02, is depicted in Figure 12 and used for transformation using methods such as those described herein. Now with reference to Figure 13, FAD2-1 5'UTR-3'UTR sequences of soybean (SEQ ID NOs: 6 and 5, linked together), FATB-1 5'UTR-3'UTR (SEQ ID NOs: 37 and 36, linked together), FAD3-1 A 3'UTR (SEQ ID NO: 16), and FAD3-1 B 5 'UTR-3'UTR (SEQ ID NOs: 27 and 26, linked together ) and amplified via PCR to result in PCR products that include re-designed restriction sites at both ends. The PCR products are cloned directly, in sense orientation, into a vector containing the soybean 7Soc 'promoter and a 3' tml terminator sequence, in the form of Xhol sites designed on the 5 'ends of the PCR primers. The vectors are then cut with Notl and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and an E9 3 'termination sequence Rubisco of pea. A vector containing a C. pulcherrima KAS IV gene (SEQ ID NO: 39) regulated by a Napin Brassica promoter and a 3 'termination sequence Napin Brassica is cut with appropriate restriction enzymes, and ligated into pMON41 164. The resulting gene expression construct, 07, is depicted in Figure 13 and used for transformation using methods like those described here. Intron 1 FAD2-1 of soybean (SEQ ID NO: 1 or 2) is amplified via PCR to result in PCR products that include re-designed restriction sites at both ends. The PCR products are cloned directly, in sense orientation, into a vector containing the soybean 7Sa 'promoter and a 3' tml termination sequence, in the form of Xhol sites designed on the 5 'ends of the PCR primers. FATB-1 5'UTR-3'UTR of soybeans (SEQ ID NOs: 37 and 36, linked together), FAD3-1 A 3'UTR (SEQ ID NO: 16), and FAD3-1 B 5 'UTR-3'UTR (SEQ ID NOs: 27 and 26, linked together) are amplified via PCR to result in PCR products that include re-designed restriction sites at both ends. The PCR products are cloned directly, in sense orientation, into a vector containing the soybean 7Sot promoter and a 3 'nos termination sequence, as Xhol sites designed on the 5' ends of the PCR primers. The vectors are then cut with Notl and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and an E9 3 'end sequence Rubisco of pea. A vector containing a C. pulcherrima KAS IV gene (SEQ ID NO: 39) regulated by a Napin Brassica promoter and a 3 'napin termination sequence Brassica is cut with appropriate restriction enzymes, and ligated into pMON41 164. The resulting gene expression construct, 09, is depicted in Figure 13 and used for transformation using methods such as those described herein. Now referring to FIGS. 19A-19C, non-coding sequences of soybean FATB-2 (SEQ ID NOs: 44-47), non-coding sequences FAD2-1 (SEQ ID NOs: 1 and 5-6), and non-coding FATB-1 sequences (SEQ ID NOs: 29-37) are amplified via PCR to result in PCR products that include re-engineered restriction sites at both ends. The PCR products are cloned directly, in sense orientation, into a vector containing the soybean 7Sa 'promoter and a 3' tml termination sequence, in the form of Xhol sites designed on the 5 'ends of the PCR primers. The vectors are then cut with Notl and ligated into p ON80612, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and an E9 3 'end sequence Rubisco of peas. The resulting gene expression construct is depicted in Figure 19A and used for transformation using methods such as those described herein. A DNA sequence containing a delta-9 desaturase is regulated by a 7S alpha promoter and a 3 'TML termination sequence is cut using the appropriate restriction enzymes and ligated into the above expression construct. The resulting expression construct is depicted in Figure 19B and used for transformation using methods described herein.

A vector containing a C. pulcherrima KAS IV gene (SEQ ID NO: 39) regulated by a bean arcelin promoter and a 3 'napin termination sequence is cut with appropriate restriction enzymes, and ligated into the above expression construct . The resulting gene expression construct is depicted in Figure 19C and used for transformation using methods as described herein. Now with reference to Figures 20A-20C, non-coding sequences of soybean FATB-2 (SEQ ID NOs: 44-47), non-coding sequences FAD2-1 (SEQ ID NOs: 1 and 5-6), non-coding sequences FATB-1 (SEQ ID NOs: 29-37), FAD3-1 A (SEQ ID NOs: 7-13 and 16-17), and non-coding sequences FAD3-1 B (SEQ ID NOs: 19 -27) are amplified via PCR to result in PCR products that include re-designed restriction sites at both ends. The PCR products are cloned directly, in sense orientation, into a vector containing the soybean 7Sa 'promoter and a 3' tml termination sequence, in the form of Xhol sites designed on the 5 'ends of the PCR primers. The vectors are then cut with Notl and ligated into pMON80612, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and an E9 3 'end sequence Rubisco of pea. The resulting gene expression construct is depicted in Figure 20A and used for transformation using methods such as those described herein. A DNA sequence containing a delta-9 desaturase is regulated by a 7S alpha promoter and a TML 3 'terminator sequence is cut using the appropriate restriction enzymes and ligated into the above expression construct. The resulting expression construct is depicted in Figure 20B and used for transformation using methods described herein. A vector containing a C. pulcherrima KAS IV gene (SEQ ID NO: 39) regulated by a Brassica bean arcelin promoter and a 3 'napin termination sequence is cut with appropriate restriction enzymes, and ligated into the expression construct previous. The resulting gene expression construct is depicted in Figure 20C and used for transformation using methods as described herein. pMON93501 contains a soybean FAD2-1 A intron (SEQ ID NO: 1) operably linked to a soybean 7Sa 'promoter and a 3' H6 terminator sequence, a C. pulcherrima KAS IV gene (SEQ ID NO: 39) operably linked to a Brassica napin promoter and a 3 'termination sequence Brassica napin, the delta 9 desaturase gene R. communis (US Patent Application Publication No. 2003/002299 8 A1) operably to a 7SA soybean promoter and a 3 'nos termination sequence, and an operably linked CP4 EPSPS gene to an eFMV promoter (a constitutive promoter derived from a escrofularia mosaic virus) and a Rubisco E9 3 'end sequence of whole pea flanked by T-DNA Agrobacterium border elements, ie right border DNA (RB) and DNA from left edge (LB). The resulting gene expression construct is used for transformation using methods as described herein. 3C. Antisense Constructs Figure 14 depicts nucleic acid molecules of the present invention wherein the first sets of DNA sequences are capable of expressing antisense constructs, and Figures 15 to 18C represent nucleic acid molecules of the present invention wherein the first sets of DNA sequences are capable of expressing combinations of sense and antisense constructs. The second set of DNA sequences comprises coding sequences, each of which is a DNA sequence encoding a sequence that when expressed is capable of increasing one or both of the protein and transcript encoded by a gene selected from the group It consists of KAS I, KAS IV, delta-9 desaturase, and CP4 EPSPS. Each coding sequence is associated with a promoter, which is any functional promoter in a plant, or any plant promoter, and can be an FMV promoter, a napin promoter, a 7S promoter (7Sot or 7S '), an arcelin promoter, a delta-9 desaturase promoter, or an FAD2-1A promoter. Now with reference to Figure 14, FAD2-1 3'UTR sequences of soybean (SEQ ID NO: 5), FATB-1 3'UTR (SEQ ID NO: 36), and FAD3-1 A 3'UTR ( SEQ ID NO: 16) are amplified via PCR to result in PCR products that include re-designed restriction sites at both ends. The PCR products are cloned directly, in sense orientation, into a vector containing the soybean 7S 'promoter and a 3' tml termination sequence, as Xhol sites designed on the 5 'ends of the PCR primers. The vectors are then cut with Notl and ligated into pMON41 64, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and an E9 3 'end sequence Rubisco of pea. The resulting gene expression construct, pMON80615, is depicted in Figure 14 and used for transformation using methods such as those described herein. Sequences FAD2-1 3'UTR of soybean (SEQ ID NO: 5), FATB-1 3 'UTR (SEQ ID NO: 36), and FAD3-1 A 3'UTR (SEQ ID NO: 16) are amplified via PCR to result in PCR products that include re-designed restriction sites at both ends. The PCR products are cloned directly, in sense orientation, into a vector containing the soybean 7Sa promoter and a 3 'tml termination sequence, in the form of Xhol sites designed on the 5' ends of the PCR primers. The vector is then cut with Notl and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and a 3 'E9 termination sequence. Rubisco of pea. A vector containing a C. pulcherrima KAS IV gene (SEQ ID NO: 39) regulated by a napin Brassica promoter and a 3 'termination sequence napin Brassica is cut with appropriate restriction enzymes, and ligated into pMON41 164. The construct The resulting gene expression, pMON80616, is depicted in Figure 14 and used for transformation using methods such as those described herein. Sequences FAD2-1 3'UTR of soybeans (SEQ ID NO: 5), FATB-1 3 'UTR (SEQ ID NO: 36), and FAD3-1 A 3'UTR (SEQ ID NO: 16) are amplify via PCR to result in PCR products that include re-designed restriction sites at both ends. The PCR products are cloned directly, in sense orientation, into a vector containing the soybean 7Sa 'promoter and a 3' tml termination sequence, in the form of Xhol sites designed on the 5 'ends of the PCR primers. The vector is then cut with Notl and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and a 3 'E9 termination sequence. Rubisco of pea. A vector containing a R. communis delta-9 desaturase (FAB2) gene (SEQ ID NO: 40) regulated by a soybean FAD2 promoter and a 3 'nos termination sequence, is cut with appropriate restriction enzymes, and is ligated into pMON41 164. The resulting gene expression construct, pMON80617, is depicted in Figure 14 and used for transformation using methods such as those described herein. Sequences FAD2-1 3'UTR of soybean seed (SEQ ID NO: 5), FATB-1 3 'UTR (SEQ ID NO: 36), and FAD3-1 A 3'UTR (SEQ ID NO: 16) are amplified via PCR to result in PCR products that include re-engineered restriction sites at both ends. The PCR products are cloned directly, in sense orientation, into a vector containing the soybean 7Sa 'promoter and a 3' tml termination sequence, in the form of Xhol sites designed on the 5 'ends of the PCR primers. The vector is then cut with Notl and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and a sequence of Termination E9 3 'Pea Rubisco. The resulting gene expression construct, pMON80630, is depicted in Figure 14 and used for transformation using methods such as those described herein. FAD2-1 5'UTR-3'UTR sequences of soybeans (SEQ ID NOs: 6 and 5, linked together), FATB-1 5'UTR-3'UTR (SEQ ID NOs: 37 and 36, linked in set), FAD3-1 A 3'UTR (SEQ ID NO: 16), and FAD3-1 B 5'UTR-3'UTR (SEQ ID NOs: 27 and 26, linked together), are amplified via PCR to result in PCR products that include re-designed restriction sites at both ends. The PCR products are cloned directly, in sense orientation, into a vector containing the soybean 7S 'promoter and a 3' tml termination sequence, as Xhol sites designed on the 5 'ends of the PCR primers. The vector is then cut with Notl and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and a 3 'E9 termination sequence. Rubisco of pea. A vector containing a C. pulcherrima KAS IV gene (SEQ ID NO: 39) regulated by a Napin Brassica promoter and a 3 'termination sequence napin Brassica is cut with appropriate restriction enzymes, and ligated into pMON41 164. The construct of The resulting gene expression, 08, is depicted in Figure 14 and used for transformation using methods such as those described herein. Now with reference to Figure 15, FAD2-1 5'UTR-3'UTR sequences of soybean (SEQ ID NOs: 6 and 5, linked together), FAD3-1 A 5'UTR-3'UTR (SEQ ID NOs: 17 and 16 linked together), and FATB-1 5'UTR-3'UTR (SEQ ID NOs: 37 and 36, linked together) are amplified via PCR to result in PCR products that include re-designed restriction sites at both ends. The PCR products are cloned directly in sense and antisense orientation in a vector containing the soybean 7Soc 'promoter and a 3' tml termination sequence, with an additional 7Sa soybean promoter located between the sense and antisense sequences, in the manner of Xhol sites designed on the 5 'ends of the PCR primers. The vector is then cut with Notl and ligated into pMON41 164, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and a 3 'E9 termination sequence. Rubisco of pea. The resulting gene expression construct, 03, is depicted in Figure 15 and used for transformation using methods such as those described herein. FAD2-1 5'UTR-3'UTR sequences of soybean seeds (SEQ ID NOs: 6 and 5, linked together), FAD3-1A 5'UTR-3'UTR (SEQ ID NOs: 27 and 26, linked in set), and FATB-1 5'UTR-3'UTR (SEQ ID NOs: 37 and 36, linked together) are amplified via PCR to result in PCR products that include re-engineered restriction sites at both ends. The PCR products are cloned directly in sense and antisense orientation in a vector containing the soybean 7Soc 'promoter and a 3' tml terminator sequence, with an additional 7Soc soybean promoter located between the sense and antisense sequences, in the manner of Xhol sites designed on the 5 'ends of the PCR primers. The vector is then cut with Notl and ligated into pMON41 164, a vector containing the CP4 gene EPSPS regulated by the FMV promoter and an E9 3 'finishing sequence Rubisco de pea. A vector containing a C. pulcherrima KAS IV gene (SEQ ID NO: 39) regulated by a Napin Brassica promoter and a 3 'termination sequence Brassica napin is cut with appropriate restriction enzymes, and ligated in pMON41 164. The resulting gene expression construct, 04, is depicted in Figure 15 and used for transformation using methods such as those described herein. Now with reference to Figures 16A-16C, non-coding sequences of soybean FATB-2 (SEQ ID NOs: 44-47), non-coding sequences FATB-1 (SEQ ID NOs: 29-37), and sequences of non-coding FAD2-1 (SEQ ID NOs: 1 and 5-6) are amplified via PCR to result in PCR products that include re-designed restriction sites at both ends. The PCR products are cloned directly in sense and antisense orientation in a vector containing the soybean 7Sa 'promoter and a 3' tml terminator sequence. The vector is then cut with an appropriate endonuclease and ligated into pMON80612, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and a 3 'E9 termination sequence. Pea Rubisco. The resulting gene expression construct is depicted in Figure 16A and used for transformation using methods as described herein. A DNA sequence containing a delta-9 desaturase is regulated by a 7S alpha promoter and a 3 'TML termination sequence is cut using the appropriate restriction enzymes and ligated into the construct of previous expression. The resulting expression construct is depicted in Figure 16B and used for transformation using methods described herein. A vector containing a C. pulcherrima KAS IV gene (SEQ ID NO: 39) regulated by a bean arcelin promoter and a 3 'napin termination sequence is cut with appropriate restriction enzymes, and ligated into the above expression construct . The resulting gene expression construct is depicted in Figure 16C and used for transformation using methods as described herein. Now referring to FIGS. 17A-17C, non-coding sequences of soybean FATB-2 (SEQ ID NOs: 44-47), non-coding sequences FATB-1 (SEQ ID NOs: 29-37), sequences of non-coding FAD2-1 (SEQ ID NOs: 1 and 5-6), and non-coding sequences FAD3-A (SEQ ID NOs: 7-3 and 16-17) are amplified via PCR to result in PCR products including sites Restricted restriction on both ends. The PCR products are cloned directly in sense and antisense orientation in a vector containing the soybean 7Soc 'promoter and a 3' tml terminator sequence. The vector is then cut with an appropriate restriction endonuclease and ligated into pMON80612, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and an E9 3 'end sequence Rubisco of pea. The resulting gene expression construct is depicted in Figure 7A and used for transformation using methods such as those described herein. A DNA sequence containing a delta-9 desaturase is Regulated by a 7S alpha promoter and a TML 3 'terminator sequence is cut using the appropriate restriction enzymes and ligated into the above expression construct. The resulting expression construct is depicted in Figure 17B and used for transformation using methods described herein. A vector containing a C. pulcherrima KAS IV gene (SEQ ID NO: 39) regulated by a bean arcelin promoter and a 3 'napin termination sequence is cut with appropriate restriction enzymes, and ligated into the above expression construct. The resulting gene expression construct is depicted in Figure 17C and used for transformation using methods as described herein. Now with reference to FIGS. 18A-18C, non-coding sequences of soybean FATB-2 (SEQ ID NOs: 44-47), non-coding sequences FATB-1 (SEQ ID NOs: 29-37), sequences of non-coding FAD2-1 (SEQ ID NOs: 1 and 5-6), and non-coding sequences FAD3-1 A (SEQ ID NOs: 7-13 and 16-17) and non-coding sequences FAD3-B (SEQ ID NOs: 19-27) are amplified via PCR to result in PCR products that include re-designed restriction sites at both ends. The PCR products are cloned directly in sense and antisense orientation in a vector containing the soybean 7Sct 'promoter and a 3' tml terminator sequence. The vector is then cut with an appropriate restriction endonuclease and ligated into pMON80612, a vector containing the CP4 EPSPS gene regulated by the FMV promoter and an E9 3 'end sequence Rubisco of pea. The resulting gene expression construct is depicted in Figure 18A and used for transformation using methods such as those described herein. A DNA sequence containing a delta-9 desaturase is regulated by a 7S alpha promoter and a 3 'TML termination sequence is cut using the appropriate restriction enzymes and ligated into the above expression construct. The resulting expression construct is depicted in Figure 18B and used for transformation using methods described herein. A vector containing a C. pulcherrima KAS IV gene (SEQ ID NO: 39) regulated by a bean arcelin promoter and a 3 'napin termination sequence is cut with appropriate restriction enzymes, and ligated into the above expression construct. The resulting gene expression construct is depicted in Figure 18C and used for transformation using methods as described herein. The nucleic acid molecules described above are preferred embodiments that achieve the objectives, characteristics and advantages of the present invention. The present invention is not intended to be limited to the illustrated embodiments. The arrangement of the sequences in the first and second sets of DNA sequences within the nucleic acid molecule is not limited to the illustrated and described arrangements, and can be altered in any suitable manner to achieve the objectives, characteristics and advantages of the invention. present invention as described herein, illustrated in the accompanying drawings, and included within the claims. 3D In vivo assembly One aspect of the present invention includes a DNA construct that is assembled within a recombinant transcription unit on an in plant plant chromosome capable of forming double-stranded RNA. The assembly of said constructs and methods for the in vivo assembly of a recombinant transcription unit for gene suppression are described in International Application No. PCT / US2005 / 00681, hereby incorporated by reference in its entirety. pMON93505 is a construct used for in vivo assembly and has two T-DNA segments, each flanked by T-DNA Agrobacterium border elements, ie right border DNA (RB) and left border DNA (LB). The first T-DNA contains a soybean 7Sot 'promoter operably linked to a soybean FAD2-1 A intron 1 (SEQ ID NO: 1), which is reduced by 100 contiguous nucleotides of the 3' end and ligated to the FATG-1 a3 'UTR followed by a FATB-1 a5' UTR, a C. pulcherrima KAS IV gene (SEQ ID NO: 39) operably linked to a Brassica napin promoter and a 3 'termination sequence Brassica napin, a delta gene 9 desaturase Ricinus communis (US Patent Application Publication No. 2003/00229918 A1) operably to a 7SA soybean promoter and a 3 'nos terminus sequence, and a CP4 EPSPS gene operably linked to an eFMV promoter and a Rubisco E9 3 'termination sequence of whole pea flanked by T-DNA border elements Agrobacterium, ie right border DNA (RB) and left border DNA (LB). At same construct, in the second T-DNA segment, flanked by another RB and LB, is a 3 'H6 terminator sequence operably linked to a soy intron 1 FAD2-1 A (SEQ ID NO: 1), which is reduced by 100 contiguous nucleotides of the 3 'end and ligated to the FATB-1 a3' UTR followed by a FATB-1 a5 'UTR. The gene expression construct is used for transformation using methods as described herein. When the two T-DNA segments of the above construct are inserted into a single site of the chromosome of a host organism in an RB to RB orientation, the assembled transcription unit has a soybean 7Sa 'promoter operably linked to intron 1 FAD2 -1 A of soybean oriented sense and anti-sense and DNA fragments FATB-1 a. When transcribed the operably linked sense and anti-sense oriented RNA sequences are capable of forming double-stranded RNA for deletion of FAD2-1 A and FATB. pMON93506 is a construct used for in vivo assembly that has two T-DNA segments, each flanked by T-DNA Agrobacterium border elements, right border DNA (RB) and left border DNA (LB). The first T-DNA segment contains a soybean 7Sa 'promoter operably linked to a soybean FAD2-1A intron 1 (SEQ ID NO: 1), which is reduced by 100 contiguous nucleotides of the 3' end and ligated to the FATB-1 a3 'UTR followed by a FATB-1 a5' UTR, a Ricinus communis delta 9 desaturase gene (U.S. Patent Application Publication No. 2003/00229918 A1) operably linked to a 7SA promoter of soybean seed and a 3 'end sequence, and a CP4 EPSPS gene operably linked to an eFMV promoter and a Rubisco E9 3' end sequence of whole pea flanked by TB and RB. In the same vector, in the second T-DNA segment, it is a 3 'H6 terminator sequence operably linked to a soybean FAD2-1 A intron 1 (SEQ ID NO: 1), which is reduced by 100 contiguous nucleotides of the extreme 3 'and is linked to FATB-1 a3' UTR followed by a FATB-1 a5 'UTR, flanked by another RB and LB. The resulting gene expression construct is used for transformation using methods as described herein. When the two T-DNA segments of the above construct are inserted into a single site of the chromosome of a host organism in an RB to RB orientation, the assembled transcription unit has a soybean 7Sa 'promoter operably linked to intron 1 FAD2 -1 A of soybean oriented sense and anti-sense and DNA fragments FATB-1 a. When they are transcribed, operably linked sense and anti-sense oriented RNA sequences are capable of forming double-stranded RNA for suppression of FAD2-1 A and FATB. pMON95829 is a construct used for in vivo assembly that has two T-DNA segments, each flanked by Agrobacterium T-DNA border elements, ie right border DNA (RB) and left border DNA (LB). The first T-DNA contains a soybean 7Sa 'promoter operably linked to a soybean FAD2-1 A intron 1 (SEQ ID NO: 1), which is reduced by 100 contiguous nucleotides of the 3' end and ligated to 42 contiguous nucleotides of a FATG-1 a5 'UTR, followed by the coding region ("CTP") of transit peptide FATB-1 acloroplast and a CP4 EPSPS gene operably linked to an EFMV promoter and a Rubisco termination sequence E9 3 'whole pea flanked by T-DNA Agrobacterium border elements, ie right border DNA (RB) and left border DNA (LB). In the same vector, in the second T-DNA segment, flanked by another RB and LB, is a 3 'H6 terminator sequence operably linked to a soybean FAD2-1 A intron (SEQ ID NO: 1), which is reduced by 100 contiguous nucleotides of the 3 'end and ligated to 42 contiguous nucleotides of a FATB-1 a5' UTR, followed by the coding region ("CTP") of transit peptide FATB-1 acyloroplast. The resulting gene expression construct is used for transformation using methods as described herein. When the two T-DNA segments of the above construct are inserted into a single site of the chromosome of a host organism in an RB to RB orientation, the assembled transcription unit has a soybean 7Sa 'promoter operably linked to intron 1 FAD2 -1 A of soybean oriented sense and anti-sense and DNA fragments FATB-1 a. When transcribed the operably linked sense and anti-sense oriented RNA sequences are capable of forming double-stranded RNA for deletion of FAD2-1 A and FATB. pMON97595 is a construct used for in vivo assembly and has two T-DNA segments, each flanked by T-border elements.

Agrobacterium DNA, that is, right border DNA (RB) and left border DNA (LB). The first T-DNA segment contains a 7Sa 'soybean promoter operably linked to a soybean FAD2-1A intron 1 (SEQ ID NO: 1), which is reduced by 320 contiguous nucleotides of the 3' end and ligated to 42 contiguous nucleotides of a FATB-1 a5 'UTR, followed by the coding region ("CTP") of transit peptide FATB-1 acloroplast and a CP4 EPSPS gene operably linked to an EFMV promoter and a Rubisco E9 termination sequence 3 The entire pea is flanked by T-DNA Agrobacterium border elements, ie right border DNA (RB) and left border DNA (LB). In the second T-DNA segment, flanked by another RB and LB, is a 3 'H6 terminator sequence operably linked to a soybean FAD2-1 A intron 1 (SEQ ID NO: 1), which is reduced by 320 contiguous nucleotides of the 3 'end and ligated to 42 contiguous nucleotides of a FATB-1 a5' UTR, followed by the coding region FATB-1 aCTP. The resulting gene expression construct is used for transformation using methods as described herein. When the two T-DNA segments of the above construct are inserted into a single site of the chromosome of a host organism in an RB to RB orientation, the assembled transcription unit has a soybean 7Sa 'promoter operably linked to intron 1 FAD2 -1 A of soybean oriented sense and anti-sense and DNA fragments FATB-1 a. When transcribed, operably linked sense and anti-sense oriented RNA sequences are capable of forming chain RNA double for suppression of FAD2-1 A and FATB. pMON97581 is a construct used for in vivo assembly and has two T-DNA segments, each flanked by T-DNA Agrobacterium border elements, ie right border DNA (RB) and left border DNA (LB). The first T-DNA segment contains a soybean 7Sa 'promoter operably linked to a soybean FAD2-1A intron 1 (SEQ ID NO: 1), which is reduced by 320 contiguous nucleotides of the 3' end and ligated to the coding region ("CTP") of transit peptide FATB-1 acloroplast and a CP4 EPSPS gene operably linked to an EFMV promoter and a Rubisco E9 3 'end sequence of whole pea flanked by T-DNA Agrobacterium border elements, that is, right border DNA (RB) and left border DNA (LB). In the same construct, in the second T-DNA segment, flanked by another RB and LB, is a 3 'H6 terminator sequence operably linked to a soy FAD2-A intron 1 (SEQ ID NO: 1), which is reduced by 320 contiguous nucleotides of the 3 'end and binds to the FATB-1aCTP coding region. The resulting gene expression construct is used for transformation using methods as described herein. When the two T-DNA segments of the above construct are inserted into a single site of the chromosome of a host organism in an RB to RB orientation, the assembled transcription unit has a soybean 7Sa 'promoter operably linked to intron 1 FAD2 - A soybean seed oriented sense and anti-sense and DNA fragments FATB-1 a. When transcribed the operably linked sense and anti-sense oriented RNA sequences are capable of forming double-stranded RNA for deletion of FAD2-1 A and FATB. pMON97596 is a construct used for in vivo assembly that has two T-DNA segments, each flanked by T-DNA border elements Agrobacterium, ie right border DNA (RB) and left border DNA (LB). The first T-DNA segment contains a soybean 7Sa 'promoter operably linked to a soybean FAD2-1 A intron 1 (SEQ ID NO: 1), which is reduced by 320 contiguous nucleotides of the 3' end and ligated at 5'180bp of the coding region ("CTP") of transit peptide FATB-1 acloroplast and a CP4 EPSPS gene operably linked to an EFMV promoter and a Rubisco E9 3 'end sequence of whole pea flanked by border elements T-DNA Agrobacterium, ie right-edge DNA (RB) and left-border DNA (LB). In the same construct, in the second T-DNA segment, flanked by another RB and LB, is a 3 'H6 terminator sequence operably linked to a soybean FAD2-1A intron 1 (SEQ ID NO: 1), which is reduced by 320 contiguous nucleotides of the 3 'end and ligated to 5' 180 bp of the coding region FATB-1 aCTP. The resulting gene expression construct is used for transformation using methods as described herein. When the two T-DNA segments of the previous construct are inserted into a single site of the chromosome of a host organism in an RB to RB orientation, the assembled transcription unit has a 7Sa soybean promoter operably linked to intron 1 sense and anti-sense oriented soybean FAD2-1 A and DNA fragments FATB-1 a. When transcribed the operably linked sense and anti-sense oriented RNA sequences are capable of forming double-stranded RNA for deletion of FAD2-1 A and FATB. pMON97597 is a construct used for in vivo assembly that has two T-DNA segments, each flanked by Agrobacterium T-DNA border elements, ie right border DNA (RB) and left border DNA (LB). The first T-DNA segment contains a soybean 7Sa 'promoter operably linked to a soybean FAD2-1 A intron 1 (SEQ ID NO: 1), which is reduced by 320 contiguous nucleotides of the 3' end and ligated at 5 '120bp of the coding region ("CTP") of transit peptide FATB-1 acloroplast and a CP4 EPSPS gene operably linked to an EFMV promoter and a Rubisco E9 3' termination sequence of whole pea flanked by border elements T-DNA Agrobacterium, ie right-edge DNA (RB) and left-border DNA (LB). In the same construct, in the second T-DNA segment, flanked by another RB and LB, is a 3 'H6 terminator sequence operably linked to a soy intron 1 FAD2-1 A (SEQ ID NO: 1), which is reduced by 320 contiguous nucleotides of the 3 'end and ligated to 5' 120bp of the coding region FATB-1 aCTP. The resulting gene expression construct is used for transformation using methods as described herein. When the two segments of T-DNA of the previous construct are inserted into a single chromosome site of a host organism in an RB to RB orientation, the assembled transcription unit has a soybean 7Sa 'promoter operably linked to intron 1 sense and anti-soybean intron 1 FAD2-1 A sense and fragments DNA FATB-1 a. When transcribed the operably linked sense and anti-sense oriented RNA sequences are capable of forming double-stranded RNA for suppression of FAD2-A and FATB. pMON97598 is a construct used for in vivo assembly that has two T-DNA segments, each flanked by Agrobacterium T-DNA border elements, ie right border DNA (RB) and left border DNA (LB). The first T-DNA segment contains a soybean 7Sa 'promoter operably linked to a soybean FAD2-1 A intron 1 (SEQ ID NO: 1), which is reduced by 340 contiguous nucleotides of the 3 'end and ligated to the coding region ("CTP") of transit peptide FATB-1-chloroplast and a CP4 EPSPS gene operably linked to an EFMV promoter and a Rubisco E9 termination sequence 3 The entire pea is flanked by T-DNA Agrobacterium border elements, ie right border DNA (RB) and left border DNA (LB). In the same construct, in the second T-DNA segment, flanked by another RB and LB, is a 3 'H6 terminator sequence operably linked to a soy intron 1 FAD2-1 A (SEQ ID NO: 1), which is reduces by 340 contiguous nucleotides of the 3 'end and binds to the coding region FATB-1 aCTP. The resulting gene expression construct is used for transformation using methods as described here. When the two T-DNA segments of the above construct are inserted into a single site of the chromosome of a host organism in an RB to RB orientation, the assembled transcription unit has a soybean 7Sa 'promoter operably linked to intron 1 FAD2 -1 A of soybean oriented sense and anti-sense and DNA fragments FATB-1 a. When transcribed the operably linked sense and anti-sense oriented RNA sequences are capable of forming double-stranded RNA for deletion of FAD2-1 A and FATB.

EXAMPLE 4 Plant transformation and analysis The constructs of Examples 2 and 3 are stably introduced into soybeans (for example, Asgrow A4922 or Asgrow A3244 or Asgrow A3525) by methods described at the beginning, including the methods of McCabe et al., Bio / Technology, 6: 923-926 (1988), or transformation mediated by Agrobacterium. Transformed soybean plants are identified by selection in medium containing glyphosate. The fatty acid compositions are analyzed from the seed of soy lines transformed with the constructs using gas chromatography. In addition, any of the constructs may contain other sequences of interest, as well as different combinations of promoters.

For some applications, modified fatty acid compositions are detected in seed development, while in other cases, such as for oil profile analysis, detection of fatty acid modifications that occur subsequently in the FAS path, or for detection of minor modifications to the fatty acid composition, analysis of fatty acid or mature seed oil is preferred. In addition, analysis of oil and / or fatty acid content of individual seeds may be desirable, especially in the detection of oil modification in the segregation of seed populations Ri. As used herein, the R0 generation indicates the plant emergence of the transformation / regeneration protocols described herein, the R1 generation indicates the growth of the seeds in the transgenic R0 plant itself. The R1 plants develop from the R1 seeds. The fatty acid compositions are determined for the seed of soybean lines transformed with the constructs of example 3. From one to ten seeds of each of the transgenic soybean and control soybean lines are ground individually using a tissue homogenizer (Pro Scientific) for the extraction of oil. The oil from the crushed soybean is extracted overnight in 1.5 ml of heptane containing triheptadecanoin (0.50 mg / ml). Aliquots of 200 μ? of the extracted oil are derived to methyl esters with the addition of 500 μ? of sodium methoxide in absolute methanol. The derivatization reaction is allowed to progress for 20 minutes at 50 ° C. The reaction is interrupted by the addition Simultaneous 500 μ? 10% (w / v) of sodium chloride and heptane 400 μ ?. The resulting fatty acid methyl esters extracted in hexane are resolved by gas chromatography (GC) in a GC 6890 Hewlett Packard (Palo Alto, CA). The GC is equipped with a Supelcowax 250 column (30 m, 0.25 mm id, 0.25 microns film thickness) (Supelco, Bellefonte, PA). Column temperature is 175 ° C at the injection and the programmed temperature from 175 ° C to 245 ° C to 175 ° C to 40 ° C / min. The temperatures of the injector and detector are 250 ° C and 270 ° Cm, respectively.

EXAMPLE 5 Synthetic fuel oil with improved biodiesel properties A synthesized fuel oil fatty acid composition is prepared having the following mixtures of fatty acid methyl esters: 73.3% oleic acid, 21.4% linoleic acid, 2.2% palmitic acid, 2.1% linolenic acid and 1.0 % stearic acid (all by weight). The purified fatty acid methyl esters are obtained from Nu-Chek prep., Inc., Elysian, MN, USA. The cetane number and the ignition delay of this composition is determined by the Southwest research Institute using an Ignition Quality Analyzer ("IQT") 613 (Southwest Research Institute, San Antonio, Texas, USA). An IQT consists of a constant volume combustion chamber that is electrically heated, an injection system fuel, and a computer that is used to control the experiment, recording the data and providing interpretation of the data. The fuel injection system includes a fuel injection nozzle that forms an entrance to the combustion chamber. A needle enhancement sensor in the fuel injection nozzle detects the fuel flow in the combustion chamber. A pressure transducer attached to the combustion chamber measures the cylinder pressure, the pressure inside the combustion chamber. The basic concept of an IQT is measured from the time of the start of fuel injection in the combustion chamber to the start of combustion. The thermodynamic conditions in the combustion chamber are precisely controlled to provide the measurement consisting of the ignition delay time. For a cetane number and ignition delay test, the test fuel is filtered using a 5-micron filter. The fuel reservoir, injection line, and nozzle are purged with pressurized nitrogen. The fuel reservoir then re-cleans with a lint-free flannel. A portion of the test fuel is used to purge the fuel reservoir, injection line, and nozzle. The reservoir is filled with the test fuel and all the air is poured out of the system. The reservoir is pressurized to 3,402 atm. The method basically consists of injecting a precisely metered quantity of the test fuel into the combustion chamber at high pressure, which is charged with air at the desired pressure and desired temperature. The measurement consists of determining the time from the start of the injection to the start of combustion, sometimes referred to as the ignition delay time. This determination is based on the measured needle enhancement and combustion chamber pressures. The normal cetane classification procedure requires the adjustment of skin temperature to 567.5 ° C and the air pressure to 2.1 MPa. A fuel with a known injection delay is run in the IQT combustion pump at the start of the day to ensure that the unit operates within normal parameters. The synthetic test then runs. The known fuel is run again to verify that the system has not changed. Once the fuel reservoir is reconnected to the fuel injection pump, the test procedure starts in the PC controller. The computer controls the entire procedure, including air loading, fuel injection, and exhaust events. 32 repeated combustion events are taken. The ignition delay is the time from the start of the injection.

It is determined from loyal needle enhancement and cylinder pressure data. The increase of injection needle signals starting from the injection. The cylinder pressure drops slightly due to the cooling effect of the vaporization of the fuel. The start of combustion is defined as the recovery time of the cylinder pressure - increases due to combustion at pressure is just before the fuel injection. The measured ignition delay times are then used to determine the cetane number based on a calibration curve which is incorporated into the data acquisition and reduction software. The calibration cu which consists of cetane number as a function of the ignition delay time, is generated using mixtures of the primary reference fuels and NEG verification fuels. In the case of test fuels that are liquid at ambient conditions, the calibration cu are verified on a daily basis using at least one known cetane number fuel check (Ryan, "Correlation of Physical and Chemical Ignition Delay to Cetane Number ", SAE Paper 852103 (1985); Ryan," Diesel Fuel Ignition Quality as Determined in a Constant Volume Combustion Bomb ", SAE Paper 870586 (1986); Ryan, "Development of a Portable Fuel Cetane Quality Monitor," Belvoir Fuels and Lubricants Research Facility Report No. 277, May (1992); Ryan, "Engine and Constant Volume Bomb Studies of Diesel Ignition and Combustion", SAE Paper 881616 (1988); and Allard et al., "Diesel Fuel Ignition Quality as Determined in the Ignition Quality Tester (" IQT ")", SAE Paper 961 182 (1996)). As shown in Table 3, the synthesized oily composition exhibits cetane numbers and ignition delays which are suitable for use, for example, without limitation, as a biodiesel oil.

TABLE 3 1 The fuel called "High Check" is a calibration fuel. It can have a cetane number of 49.3 ± 0.5. The unit is verified with the calibration before and after the running of the synthetic test fuel. The fog point density (ASTM D-4052) (ASTM D-2500), pour point (ASTM D-97), and cold filter plugging point (IP 309 / ASTM D-6371) are determined for the synthesis of oil using ASTM D protocols. ASTM D protocols are obtained from ASTM, 100 Barr Harbor Drive, West Conshohocken, PA, USA. The results of these tests are set forth in Table 4. As shown in Table 4, the synthesized oil composition exhibits numbers that are suitable for use as, for example, without limitation as a biodiesel oil.

TABLE 4 Nitric oxide emission levels are estimated by evaluating the levels of unsaturation of a biologically based fuel by measuring the fuel density and directly calculating the estimated emission levels, or by measuring directly. There are also standard protocols available for levels measured directly from nitric oxide emissions. The synthesized oil is estimated to have lower nitric oxide emission levels than fatty acid methylated fatty acid esters of conventional soybean oil based on an evaluation of the overall level of instauration in the synthesized oil. Oils that contain large numbers of double bonds, that is, have a higher degree of unsaturation, tend to produce higher nitric oxide emissions. The oil has a total of 123 double bonds, as compared to the total of 153 double bonds of conventional soybean oil, as shown in table 5.

TABLE 5 SYNTHETIC OIL 73% oleic acid (18: 1) x 1 double bond = 73 22% linoleic acid (18: 2) x 2 double bonds 44 2% linolenic acid (18: 3) x 3 double bonds 6 TOTAL double bonds 123 CONVENTIONAL SOY SEED OIL 23% oleic acid (18: 1) x 1 double bond = 23 53% linoleic acid (18: 2) x 2 double bonds 106 8% linolenic acid (8: 3) x 3 double bonds 24 TOTAL double bonds 153 As reported by the National Renewable Energy Laboratory, Contract No. ACG-8-17106-02 Final report, The composition effect of biodiesel in emissions of engines of the series A DDC Series 60 Motor a Diesel, (June 2000), nitric acid emissions of biodiesel compositions are prededucen by the formula and - 46.959x - 36.388 where and are the emissions of oxide in grams / hours horsepower-brake; and x is the density of biodiesel The formula is based on regression analysis of emission data of nitric acid in a test involving 16 biodiesel fuels. The test makes use of a calibration 1991, serial production model 60 Detroit Diesel Corporation engine.

The density of the synthesized oil is determined by the Southwest Research Institute using the method ASTM D4052. The result shown in Table 4 is used in the above equation to predict an emission value of nitric oxide of 4.89 g / bhp-h. This result is compared to a control soybean product. The National Renewable Energy Laboratory report provides the density and emissions of nitric oxide from a biodiesel based on soybeans (IGT methyl ester). The density of the control biodiesel is 0.8877 g / mL, it provides a calculated nitric oxide emission of 5.30 g / bhp-h. This calculated emission value is similar to the experimental value for nitric oxide emission of 5.32 g / bhp-h. The synthesized oil composition exhibits improved numbers compared to the control and is suitable for use, for example, without limitation, as a biodiesel oil.

EXAMPLE 6 Optimal fatty acid composition for healthy serum lipid levels The cholesterol lowering properties of plant compositions are determined to identify fatty acid compositions that have a more favorable effect on serum lipid levels than conventional soybean oil (i.e., lower LDL-cholesterol and higher HDL-cholesterol). Published equations based on 27 clinical trials (Mensink, RP and Katan, MB Arteriosclerosis and Thrombosis, 12:91 1-919 (1992)) are used to compare the effects on serum lipid levels in human new seed compositions oleaginous with that of normal soybean oil. Table 6 below shows the results of the change in serum lipid levels are 30% of the energy of the carbohydrate diet is replaced by lipids. The results show that soybean oil already has favorable effects on serum lipids when it replaces carbohydrates in the diet. Improvements in this composition are possible by decreasing the levels of saturated fat and by obtaining a level of linoleic acid between 10-30% of the total fatty acids, preferably around 15-25% of the total fatty acids. When the proportion of linoleic acid is less than 10% of the total fatty acids, the new composition increases the LDL-cholesterol compared to the control soybean oil, even when the saturated fat content is decreased to 5% of the total fatty acids. When the proportion of linoleic acid is increased, the ability of the composition to increase serum HDL levels is reduced. Therefore, the preferred linoleic acid is determined to be about 15-25% of the total fatty acids.

TABLE 6 vs. control (mg / dl) 1 .00 72.00 21.00 1.00 2,000 3,000 21% 18: 2, < 3.2% sat (%) 0 0 0 0 Proportion of 30% fat 0.30 21 .60 0.30 0.600 6.300 0.900 E (%) 0 0 0 Calculation of LDL 0.38 0.16 0.768 5.184 3.465 0.216 -7.878 (mg / dl) 4 5 vs. control (mg / dl) -1,845 Calculation of HDL 0.14 0.08 0.282 7.344 1 .764 0.306 9.921 (mg / dl) 1 4 vs. control (mg / dl) 0.234 2.00 57.00 30.00 3.00 3.000 5.000 30% 18: 2, < 6% sat (%) 0 0 0 0 Proportion of 30% fat 0.60 17.10 0.90 0.900 9.000 1.500 E (%) 0 0 0 Calculation of LDL 0.76 0.49 1.152 4.104 4.950 0.360 -7.989 (mg / dl) 8 5 control (mg / dl) -1.956 Calculation of HDL 0.28 0.25 0.423 5.814 2.520 0.510 9.801 (mg / dl) 2 2 vs. control (mg / dl) 0.114 EXAMPLE 7 The following fourteen steps illustrate the construction of vector pMON68537 designated for plant transformation to suppress FAD2, FAD3 and FATB genes and overexpression of delta-9 desaturase in soybean seed. In particular, the construct comprises an alpha 7S promoter operably linked to intron oriented soybean sense and 3 UTR, i.e., an intron # 1 FAD2-1 A, a FAD3-1A 3'UTR, a FATB-1 3 ' UTR, an intron that can be spliced of fork loop formation, and an antisense oriented intron of soybean and 3'UTR, that is, an FA TB-1 3'UTR, an FAD3- 1A 3'UTR and a Intron # 1 FAD2- 1A and the FAD2 promoter of soybean that triggers the delta-9 desaturase.

Stage 1 The soybean intron # 5 FAD3-1 A, which serves as the spliced intron portion of the RNAids construct, is PCR amplified using genomic soybean DNA as the template, with the following primers. 5 'primer = 19037 ACTAGTATATTGAGCTCATATTCCACTGCAGTGGATATT GTTTAAACATAGCTAGCATATTACGCGTATATTATACAAGCTTATATTCCC GGGATATTGTCGACATATTAGCGGTACATTTTATTGCTTATTCAC 3' primer = 19045 ACTAGTATATTGAGCTCATATTCCTGCAGGATATTCTCGAG ATATTCACGGTAGTAATCTCCAAGAACTGGTTTTGCTGCTTGTGTCTGCAG TGAATC.

These primers add cloning sites to the 5 'and 3' ends. To the extreme 5 ': Spel, Sacl, BstXI, Pmel, Nhel, Mlul, HindIII, Xmal, Smal, Salí. To the 3 'end: Spel, Sacl, Sse8387l, Xhol. The intron PCR product # 5 from soybean FAD3-1A is cloned into pCR2.1, resulting in KAWHIT03.0065. KAWHIT03.0065 are then digested with Spel and the ends are filled with Pfu polymerase and pMON68526 (empty chloramphenicol (hereinafter CM) resistant vector) is digested with HindIII and the ends are filled with Pfu polymerase. KAWHIT03.0065 and pMON68526 are then linked to create pMON68541 (intron # 5 F4D3-1 A soybean seed with multiple cloning sites in the resistant vector Amp).

Stage 2 The FA TB-1 3'UTR of soybean is amplified with the following primers: 18662 = TTTTAATTACAATGAGAATGAGATTTACTGC (add Bsp120l to the 5 'end) and 18661 = GGGCCCGATTTGAAATGGTTAACG. The PCR product is then ligated into pCR2.1 to make KAWHIT03.0036.

Step 3 KAWHIT03.0036 is then digested with Bsp120l and EcoRI and then cloned into KAWHIT03.0032 (CM resistant vector empty with a multiple cloning site) to make KAWHIT03.0037 (FATB-1 3'UTR in CM resistant vector empty) .

Step 4 The FACfcA h 3'UTR of soybean is amplified with the following primers: 18639 = GGGCCCGTTTCAAAC I I I I GG (add Bsp120l to the 5 'end) and 18549 = TGAAACTGACAATTCAA. The PCR product is then ligated into pCR2.1 to make KAWHIT03.0034.

Stage 5 KAWHIT03.0034 is digested with Bsp120l and EcoRI and then ligated into KAWHIT03.0032 (CM resistant vector with a multiple cloning site) to make KAWHIT03.0035 (F4D3-1 A 3'UTR in CM resistant vector empty).

Step 6 Intron # 1 FAD 2-1 A soybean is PCR amplified using genomic DNA from soybean seed as template, with the following primers: 5 'primer = 18663 = GGGCCCGGTAAATTAAATTGTGC (Add the Bsp120l site to the 5' end); and initiator 3 * = 18664 = CTGTGTCAAAGTATAAACAAGTTCAG. The resulting PCR product is cloned into pCR 2.1 creating KAWHIT03.0038.

Stage 7 The intron # 1 of soybean FAD 2-1 A is the PCR product in KAWHIT03.0038 is cloned in KAWHIT03.0032 (vector resistant vacuum CM with a multiple cloning site) using the restriction sites Bsp 20l and EcoRI . The resulting plasmid is KAWHIT03.0039 (intron # 1 FAD 2-1 A soybean in CM resistant vector empty).

Stage 8 KAWHIT03.0039 is digested with AscI and Hindlll and pMON68541 (intron # 5FAD3- 1A AMP resistant core vectors AMP) is digested with Mlul and HindIII. The # 1 FAD 2-1 A in soybean is then directionally cloned into pMON68541 to generate KAWHIT03.0071 (intron # 1 FAD2-1A with an intron # 5 FAD3-1A soybean).

Stage 9 KAWHIT03.0035 (FAD3- 1A 3'UTR in CM resistant vector) is digested with AscI and HindIII and KAWHIT03.0071 (FAD2- 1A intron and FAD3-1A intron # 5 AMids AMP resistant base vector) is digested with Mlul and HindIII. The FAD 3-1 A 3'UTR of soybean is then directionally cloned into KAWHIT03.0071 to generate KAWHIT03.0072 (intron # 1 FAD2- 1A of soybean seed and FAD3- 1A 3'UTR with intron # 5 FAD3- 1A of soybean seed).

Step 10 KAWHIT03.0037 (FATB- † 3'UTR in CM resistant vector) is digested with AscI and HindIII and KAWHIT03.0072 is digested with Mlul and HindIII. The F47B-1 3'UTR is then cloned directionally into KAWHIT03.0072 to obtain KAWHIT03.0073 (FAD2AÍK in soybean intron, F / 4D3-1 A 3'UTR, FAT &- 3'UTR with intron # 5 ?? 03-1?).

Step 1 1 KAWHIT03.0073 is digested with BstXI and SalI and the fragment containing intron F4D2-1 A, FAD3AA 3'UTR and FAT A 3'UTR is purified gel. In a different tube KAWHIT03.0073 is digested with Xhol and Sse8387l. Thetron / 3'UTR fragment is then cloned back into KAWHIT03.0073 in the opposite orientation at the other intron site # 5 FAD3-1A of soybean seed to create KAWHIT03.0074 (intron # 1 FAD2- 1A seed of soy sense, FAD3-1A 3'UTR of soybean seed, FATB- 1 3'UTR of soybean seed, soybean seed, intron # 5 FAD3- 1A of soybean that can be spliced, FATB- 1 3 ' UTR of antisense soybean seed, FAD3- 1A 3'UTR of anti-sense soybean seed, intron # 1 FAD2- 1A anti-sense of soybean seed).

Step 12 To bind the RNAids construct to the alpha promoter 7S and the TML 3 ', KAWHIT03.0074 and pMON68527 (cassette 7Sa' / TML3 ') are digested with Sacl and ligated together to obtain pMON68563 (promoter 7S alpha' - intron # 1 FAD2- 1A sense, FAD3- 1A 3'UTR sense of soybean seed, FATB-1 3'UTR sense of soybean seed, FATB- 1 3'UTR anti-sense soybean that can be spliced, FAD3- 1A 3'UTR anti-sense of soybean seed, intron # 1 FAD2- 1A anti-sense of soybean seed - TML3 ').

Step 13: To introduce the assembled RNAids construct in pMON70682, pMON68563 and pMON70682 are digested with NotI and ligated together to form pMON68536 comprising a 7S alpha promoter operably linked to the double stranded RNA-forming construct of intron # 1 FAD2- 1A sense, FAD3- 1A 3'UTR sense of soybean seed, FATB- 1 3'UTR sense of soybean seed, intron # 5 FAD3- 1A of soybean that can be spliced, FATB- 1 3'UTR anti-sense soybean, FAD3- 1A 3'UTR anti-sense soybean, intron # 1 FAD2- 1A anti-sense soybean and terminator TML3 ').

Step 14 pMON68536 is then digested with AscI and Rsrll and pMON68529 (containing the marker select CP4 fused to the FMV promoter and the RBCS 3 'and the FAD1 promoter of soybean which drives the delta 9 desaturase) is digested with SanDI and Ascl. The mRNA portion of pMON68536 is then cloned directionally into pMON68529 to create pMON68537 (7S alpha promoter 'operably linked to the intron double stranded RNA formation construct # 1 FAD2-1A sense, FAD3-1A 3'UTR seed sense soybean, FATB- 1 3'UTR sense of soybean seed, intron # 5 FAD3- 1A of soybean that can be spliced, FATB- 1 3'UTR anti-sense soybean seed, FAD3- 1A 3'UTR anti-sense of soybean seed, intron # 1 FAD2- 1A anti-sense of soybean seed and terminator TML3 'and FAD2 promoter of soybean that triggers delta 9 desaturase).

EXAMPLE 8 The following fifteen steps illustrate the construction of vector pMON68539 (FIG. 22) designated for plant transformation to suppress FAD2, FAD3 and FATB genes and overexpression of delta-9 desaturase and the KASIV enzyme in soybean seed. In particular, the construct comprises an alpha 7S promoter operably linked to intron oriented soybean sense and 3 'UTR, i.e., an intron # 1 FAD2-1 A, a FAD3-1A 3'UTR, a FATB-1 3 'UTR, an intron that can be spliced of hairpin loop formation, and a complementary series of intron-oriented antisense soybean and 3'UTR, ie, a FATB-1 3'UTR, an FAD3-1A 3' UTR and an intron # 1 FAD2- 1A, the promoter of FAD2 of soybean that triggers the delta-9 desaturase, and the Napin promoter that drives KASIV.

Stage 1 The soybean intron # 5 FAD3-1 A, which serves as the spliced intron portion of the RNAids construct, is PCR amplified using genomic soybean DNA as the template, with the following primers. 5 'initiator = 19037 ACTAGTATATTGAGCTCATATTCCACTGCAGTGGATATTG TTTAAACATAGCTAGCATATTACGCGTATATTATACAAGCTTATATTCCCG GGATATTGTCGACATATTAGCGGTACATTTTATTGCTTATTCAC 3 'initiator = 19045 ACTAGTATATTGAGCTCATATTCCTGCAGGATATTCTCGAG ATATTCACGGTAGTAATCTCCAAGAACTGGTTTTGCTGCTTGTGTCTGCAG TGAATC. These primers add cloning sites to the 5 'and 3' ends. To the extreme 5 ': Spel, Sacl, BstXI, Pmel, Nhel, Mlul, Hindlll, Xmal, Smal, Salí. To the 3 'end: Spel, Sacl, Sse8387l, Xhol. The intron PCR product # 5 FAD3- 1A from soybean is cloned into pCR2.1, resulting in KAWHIT03.0065. KAWHIT03.0065 are then digested with Spel and the ends are filled with Pfu polymerase and pMON68526 (CM vacuum resistant vector) is digested with HindIII and the ends are filled with Pfu polymerase. KAWHIT03.0065 and pMON68526 are ligated to create p ON68541 (intron # 5 FAD3- ~ \ A soybean seed with multiple cloning sites in the resistant vector Amp).

Step 2 The soybean FATB-1 3'UTR is amplified with the following primers: 18662 = TTTTAATTACAATGAGAATGAGATTTACTGC (add Bsp120l to the 5 'end) and 18661 = GGGCCCGATTTGAAATGGTTAACG. The PCR product is then ligated into pCR2.1 to make KAWHIT03.0036.

Step 3 KAWHIT03.0036 is then digested with Bsp120l and EcoRI and then cloned into KAWHIT03.0032 (CM resistant vector empty with a multiple cloning site) to make KAWHIT03.0037. { FATB- 1 3'UTR in resistant CM vector empty).

Step 4 The FAD3-1A 3'UTR of soybean is amplified with the following primers: 18639 = GGGCCCGTTTCAAACTTTTTGG (add Bsp120l to the 5 'end) and 18549 = TGAAACTGACAATTCAA. The PCR product is then ligated into pCR2.1 to make KAWHIT03.0034.

Step 5 KAWHIT03.0034 is digested with Bsp120l and EcoRI and then ligated into KAWHIT03.0032 (CM resistant vector with a multiple cloning site) to make KAWHIT03.0035 (D3-1 A 3'UTR in CM resistant vector empty).

Step 6 Intron # 1 FAD 2-1 A soybean is PCR amplified using genomic DNA from soybean seed as template, with the following primers: 5 'primer = 18663 = GGGCCCGGTAAATTAAATTGTGC (Add the Bsp120l site to the 5' end); and initiator 3 '= 18664 = CTGTGTC AAAGTATAAAC AAGTTCAG. The resulting PCR product is cloned into pCR 2.1 creating KAWHIT03.0038.

Stage 7 The intron # 1 of soybean FAD 2-1 A is the PCR product in KAWHIT03.0038 is cloned into KAWHIT03.0032 (resistant vector empty CM with a multiple cloning site) using the restriction sites Bsp120l and EcoRI. The resulting plasmid is KAWHIT03.0039 (intron # 1 FAD 2-1 A soybean in CM resistant vector empty).

Step 8 KAWHIT03.0039 is digested with AscI and Hindlll and pMON68541 (intron 5FAD3-1A RNAids AMP resistant base vector) is digested with Mlul and Hindlll. The # 1 FAD 2-1 A intron of soybean is then cloned directionally in pMON68541 (intron # 5 FAD3-1A in vector resistant Amp with multiple cloning sites) to generate KAWHIT03.0071 (intron # 1 FAD2- 1A of soybean seed with an intron # 5 FAD3- 1A of soybean seed).

Stage 9 KAWHIT03.0035. { FAD3- 1A 3'UTR in CM resistant vector) is digested with AscI and Hindlll and KAWHIT03.0071 (FAD2- 1A intron and FAD 3-1 A intron # 5 AMids resistant base vector AMPs) is digested with Mlul and Hindlll. The FAD 3-1 A 3'UTR of soybean is then cloned in a directional manner in KAWHIT03.0071 to generate KAWHIT03.0072 (intron # 1 FAD2- 1A of soybean seed and FAD3- 1A 3'UTR with intron # 5 FAD3-1A of soybean seed).

Step 10 KAWHIT03.0037 (F4TB-1 3'UTR in resistant vector C) is digested with Ascl and Hindlll and KAWHIT03.0072 is digested with Mlul and Hindlll. The F47B-1 3'UTR is then cloned directionally into KAWHIT03.0072 to obtain KAWHIT03.0073 (F4D2-1 A soybean intron, F / AD3-1 A 3'UTR, F \ 7B-1 3 ' UTR with intron # 5 F4D3-1A).

Step 1 1 KAWHIT03.0073 is digested with BstXI and SalI and the fragment containing intron F4D2-1 A, F4D3-1 A 3'UTR and? 7? -1 3'UTR is purified gel. In a different tube KAWHIT03.0073 is digested with Xhol and Sse8387l. The intron / 3'UTR fragment is then cloned back into KAWHIT03.0073 in the opposite orientation at the other site of intron # 5 FAD3- 1A of soybean seed to create KAWHIT03.0074 (intron # 1 FAD2- 1A of soybean seed sense, FAD3- 1A 3'UTR of soybean seed, FATB- 1 3'UTR of soybean seed, soybean seed, intron # 5 FAD3- 1A of soybean that can be spliced, FATB- 1 3'UTR of antisense soybean seed, FAD3- 1A 3'UTR of anti-sense soybean seed, intron # 1 FAD2- 1A anti-sense of soybean seed).

Step 2 To bind the RNAids construct to the promoter alpha '7S and TML 3', KAWHIT03.0074 and pMON68527 (cassette 7Sa7TML3 ') are digested with Sacl and ligated together to obtain pMON68563 (promoter 7S alpha' - intron # 1 FAD2- 1A sense, FAD3-1A 3'UTR sense of soybean seed, FATB-1 3'UTR sense of soybean seed, FATB- 1 3'UTR anti-sense soybean seed that can be spliced, FAD3- 1A 3 ' UTR anti-sense of soybean seed, intron # 1 FAD2- 1A anti-sense of soybean seed - TML3 ').

Step 13 To introduce the assembled RNAids construct in pMON70682, pMON68563 and pMON70682 are digested with NotI and ligated together to form pMON68536 comprising a 7S alpha promoter operably linked to the intron double stranded RNA formation construct # 1 FAD2- 1A sense, FAD3- 1A 3'UTR sense of soybean seed, FATB-1 3'UTR sense of soybean seed, intron # 5 FAD3- 1A of soybean that can be spliced, FATB- 1 3'UTR anti-sense of soybean seed, FAD3- 1A 3'UTR anti-sense of soybean, intron # 1 FAD2- 1A antisense of soybean and terminator TML3 ').

Step 14 pMON68536 is then digested with AscI and Rsrll and pMON68529 (containing the selectable marker CP4 fused to the FMV promoter and the RBCS 3 'and the soybean FAD2 promoter that drives the delta 9 desaturase) is digested with SanDI and Ascl. The mRNA portion of pMON68536 is then cloned directionally into pMON68529 to create pMON68537 (7S alpha promoter 'operably linked to the intron double stranded RNA formation construct # 1 FAD2-1A sense, FAD3- 1A 3'UTR sense of soybean seed, FATB- 1 3'UTR sense of soybean seed, intron # 5 FAD3-1A of soybean that can be spliced, FATB- 1 3'UTR anti-sense of soybean seed, FAD3- 1A 3'UTR anti-sense of soybean seed, intron # 1 FAD2- 1A anti-sense of soybean seed and terminator TML3 'and promoter FAD2 of soybean that triggers delta 9 desaturase).

Step 15 pMON68537 is then digested with SanDI and Ascl and pMON70683 (Napin drive KaslV) is digested with Ascl and Rsrll. The Napin / KaslV fragment is cloned directionally in pMON68537 to create pMON68539 (7S alpha promoter 'operably linked to the intron double stranded RNA formation construct # 1 FAD2- 1A sense, FAD3- 1A 3'UTR seed sense of soybean, FATB- 1 3'UTR sense of soybean seed, intron # 5 FAD3-1A of soybean that can be spliced, FA TB- 1 3'UTR anti-sense of soybean seed, FAD3- 1A 3'UTR of anti-sense soybean seed, intron # 1 FAD2-1A anti-sense and a TML3 'terminator, soybean FAD2 promoter that drives the delta 9 desaturase and the promoter Napin activating KaslV.

EXAMPLE 9 This example illustrates the plant transformation to produce soy plants with suppressed genes. A transformation vector pMON68537 as prepared in Example 7 is used to introduce an intron double-stranded RNA / 3 'UTR formation construct into the soybean seed to suppress the? 12 desaturase,? 15 desaturase, and FATB genes. Vector pMON68537 also contains the delta-9 desaturase (FAB2) and the CP4 genes. The vector is stably introduced into soybeans (variety Asgrow A4922) via strain Agrobacterium tumefaciens ABI (Martinell, U.S. Patent No. 6,384,301). The selectable CP4 marker allows transformed soybean plants to be identified by selection in medium containing glyphosate herbicide. The fatty acid compositions are analyzed from the seed of soy lines transformed with the intron / 3 'UTR RNAid expression constructs using gas chromatography. Seed and grouped seed oil compositions Ri demonstrate that the mono- and polyunsaturated fatty acid compositions are altered in the oil of the seeds of transgenic soybean lines in comparison with those of the non-transformed soybean seed, ( see table 7). For example, the suppression of FAD2 provides plants with increased amount of oleic acid ester compounds; the suppression of FAD3 provides plants with diminished linolenic acid ester compounds; and the deletion of FATB provides plants with reduced saturated fatty ester compounds, for example, palmitates and stearates. The selections can be made from said lines depending on the desired relative fatty acid composition. The fatty acid compositions are analyzed from the seed of soybean lines transformed with constructs using gas chromatography.

EXAMPLE 10 This example illustrates the plant transformation to produce soy plants with suppressed genes. A transformation vector pMON68539 as prepared in Example 3 is used to introduce an intron double-stranded RNA / 3 'UTR formation construct into the soybean seed to suppress the? 12 desaturase,? 15 desaturase, and FATB genes. Vector pMON68539 also contains the KaslV and CP4 genes. The vector is stably introduced into soybeans (variety Asgrow A4922) via strain Agrobacterium tumefaciens ABI (Martinell, U.S. Patent No. 6,384,301). The CP4 selectable marker allows the transformed soybean plants to be identified by selection in glyphosate herbicide-containing medium. The fatty acid compositions are analyzed from the seed of soybean lines transformed with the intron / 3 'UTR RNAid expression constructs using gas chromatography. Seed and grouped seed oil compositions Ri demonstrate that the mono- or polyunsaturated fatty acid compositions are altered in the oil of the seeds of transgenic soybean lines as compared to those of the non-transformed soybean seed, ( see table 8). For example, the suppression of FAD2 provides plants with increased oleic acid ester compounds; the suppression of FAD3 provides plants with diminished linolenic acid ester compounds; and the deletion of FATB provides plants with reduced saturated fatty ester compounds, for example, palmitates and stearates. The selections can be made from said lines depending on the desired relative fatty acid composition. The fatty acid compositions are analyzed from the seed of soybean lines transformed with constructs using gas chromatography.

TABLE 7 Fatty acid composition of simple seeds R1 of events P ON68537 Constructo Event 18: 1 18: 3 16: 0 18: 0 18: 2 PMON68537 GM A36305 74.92 4.42 6.35 2.93 10.24 PMON68537 GM_ A36305 74.8 4.33 6.57 2.93 10.23 PMON68537 GM A36305 74.43 3.95 5.98 2.82 1 1 .81 PMON68537 GM_ A36305 73.32 3.99 6.79 3.24 1 1 .48 PMON68537 GM A36305 72.87 4.33 7.06 3.08 1 1 .7 PMON68537 GM_ A36305 16.63 9.53 13.5 4.06 55.31 PMON68537 GM_ A36305 16.52 9.61 13.92 4.24 54.79 PMON68537 GM_ A36305 15.67 9.66 13.64 4.19 55.89 PMON68537 GM A36306 77.45 3.93 6.76 2.47 8.4 PMON68537 GM_ A36306 74.51 4.38 6.58 2.47 10.94 PMON68537 GM .A36306 73.21 4.64 7.04 3.08 1 1 .04 PMON68537 GM_ .A36306 72.78 4.4 6.97 2.55 12.21 PMON68537 GM A36306 71 .67 4.76 6.94 3.25 12.2 PMON68537 GM .A36306 71 .01 4.86 7.64 3.05 12.41 PMON68537 GM .A36306 69.72 4.76 7.66 2.95 13.75 PMON68537 GM .A36306 17.41 8.88 13.35 3.85 55.63 PMON68537 GM. .A36307 77.22 3.71 6.8 2.77 8.5 PMON68537 GM. _A36307 76.79 3.65 6.76 2.85 8.75 P ON68537 GM. _A36307 71 .44 4.54 7.2 3.58 12.17 PMON68537 GM. _A36307 18.83 8.62 13.94 4.02 53.61 PMON68537 GM. _A36307 18.81 8.38 13.27 3.7 54.97 PMON68537 GM A36307 15.68 9.97 14.06 4.55 54.79 PMON68537 GM. _A36307 15.28 10.64 14.68 4.43 53.97 PMON68537 GM. _A36307 14.08 9.36 14.39 4.31 56.89 PMON68537 GM A36309 78.67 3.53 6.09 2.5 8.18 PMON68537 GM _A36309 75.43 3.96 6.7 2.53 10.3 PMON68537 GM _A36309 71 .41 4.19 6.92 2.74 13.67 PMON68537 GM A36309 70.51 4.14 6.85 3.16 14.33 PMON68537 GM _A36309 67.51 5.01 7.45 3.15 15.69 PMON68537 GM. _A36309 66.99 4.92 7.15 3.9 15.79 PMON68537 GM. _A36309 20.09 8.46 12.41 5 52.97 PMON68537 GM. _A36309 15.15 9.73 14.61 3.85 55.79 PMON68537 GM _A36310 74.28 4.77 7.31 1 .85 10.9 PMON68537 GM _A36310 74.03 5.43 8.23 1 .63 9.66 PMON68537 GM _A36310 73.07 5.09 7.37 1 .76 1 1 .75 Constructo Event 18: 1 18: 3 16: 0 18: 0 18: 2 PMON68537 GM_ A36310 71 .83 5.04 7.78 1 .86 12.54 PMON68537 GM_ A36310 68.01 6.26 9.8 1 .97 13.13 PMON68537 GM A36310 67.22 6.28 8.71 3.28 13.45 PMON68537 GM A36310 65.37 6.87 10.01 1 .94 14.9 PMON68537 GM A36310 15.76 10.09 13.4 4.28 55.52 PMON68537 GM A3631 1 77.87 3.56 5.9 2.46 9.05 PMON68537 GM_ A3631 1 75.8 3.87 5.91 2.93 10.22 PMON68537 GM A3631 1 75.61 3.71 6.21 2.56 10.75 PMON68537 GM A3631 1 73.68 4.06 6 3.09 1 1 .98 PMON68537 GM A3631 1 72.66 4.1 1 6.41 3.14 12.48 PMON68537 GM _A3631 1 70.89 4.39 6.52 3.1 1 13.93 PMON68537 GM_ A3631 1 70.82 3.97 6.52 3.18 14.29 PMON68537 GM_ A3631 1 16.67 9.39 13.65 4.44 54.77 PMON68537 GM_ A36312 78.32 4.3 6.36 1 .79 8.16 PMON68537 GM A36312 77.55 4.46 6.51 2.13 8.23 PMON68537 GM_ .A36312 77.43 4.17 6.31 1 .81 9.24 PMON68537 GM .A36312 76.98 4.29 6.25 2.27 9.05 PMON68537 GM .A36312 76.43 4.55 6.82 2.16 8.96 PMON68537 GM_ _A36312 76.38 4.5 6.46 2.04 9.54 PMON68537 GM A36312 75.25 4.27 6.41 1 .97 1 1 .06 P ON68537 GM. _A36312 18.24 9.43 13.6 3.07 54.75 PMON68537 GM A36313 80.18 4.07 6.17 2.59 5.85 PMON68537 GM _A36313 79.96 4.16 6.03 2.59 6.1 1 PMON68537 GM _A36313 78.88 3.9 5.6 2.8 7.65 PMON68537 GM _A36313 78.76 3.92 5.44 2.91 7.82 PMON68537 GM _A36313 77.64 4.22 5.88 2.9 8.25 PMON68537 GM. _A36313 76.15 4.14 6.06 3.13 9.42 PMON68537 GM A36313 19.05 8.87 13.45 3.71 54.03 PMON68537 GM. _A36313 18.47 8.46 13.13 3.63 55.41 PMON68537 GM _A36314 80.27 3.17 5.77 3.4 6.03 PMON68537 GM A3631 79.66 3.24 5.72 3.19 6.91 PMON68537 GM. _A36314 79.5 3.45 5.83 3.23 6.74 PMON68537 GM _A36314 77.42 3.52 5.76 3.57 8.42 PMON68537 GM _A36314 77.33 3.71 6.36 3.34 8.01 PMON68537 GM. A36314 76.83 3.71 6.38 3.24 8.59 PMON68537 GM. _A363 4 16.6 9.3 12.63 4.43 55.99 PMON68537 GM. _A36314 15.26 8.59 13.71 4.54 56.84 PMON68537 GM. _A36315 20.21 8.25 13.61 3.59 53.37 PMON68537 GM _A36315 17.47 9.22 13.46 3.35 55.57 PMON68537 G _A36315 16.75 9.3 13.61 3.66 55.75 PMON68537 GM _A36315 16.54 9.18 13.54 3.88 55.9 Constructo Event 18: 1 18: 3 16: 0 18: 0 18: 2 PMON68537 GM_ A36315 16.06 10.07 13.44 4.01 55.42 PMON68537 GM_ _A36315 16.05 9.58 12.82 4.25 56.29 PMON68537 GM_ A36315 15.95 10.42 13.12 3.63 55.91 PMON68537 GM_ _A36315 15.5 10.22 13.25 3.78 56.3 PMON68537 GM A36316 79.61 3.56 5.79 2.94 6.87 PMON68537 GM_ A36316 75.1 1 4.01 6.45 3.44 9.76 PMON68537 GM_ A36316 75.07 4.25 6.74 3.09 9.64 PMON68537 GM_ _A36316 73.92 3.97 6.53 3.56 10.75 PMON68537 GM A36316 17.26 9.59 13.1 4.26 54.78 PMON68537 GM_ A36316 17.15 9.03 12.81 4.04 55.97 PMON68537 GM_ A36316 16.62 9.2 13.15 3.99 56.03 PMON68537 GM_ _A36316 16.6 9.44 13.19 3.95 55.84 PMON68537 GM_ .A36317 18.96 7.55 13.2 3.75 55.51 PMON68537 GM_ _A36317 16.19 9.43 13.33 3.96 56.04 PMON68537 GM_ .A36317 16.05 9.1 14.02 3.94 55.91 PMON68537 GM_ _A36317 15.33 9.4 13.91 4.22 56.1 1 PMON68537 GM_ .A36317 15.28 9.2 13.87 4.27 56.36 PMON68537 GM_ .A36317 14.58 10.15 13.74 4.38 56.15 PMON68537 GM_ A36317 13.95 9.47 13.98 4.76 56.79 PMON68537 GM_ _A36317 13.91 9.88 14.26 4.62 56.25 PMON68537 GM. A36318 78.82 3.64 5.7 2.77 7.87 PMON68537 GM _A36318 77.94 3.73 5.9 2.94 8.29 PMON68537 GM. _A36318 75.18 4.1 1 6.08 3.48 9.95 PMON68537 GM. _A36318 75.1 3.93 6.02 3.04 10.75 PMON68537 GM. _A36318 75.01 4.22 6.57 3.29 9.72 PMON68537 GM. _A36318 74.17 4.2 6.51 3.27 10.68 PMON68537 GM _A36318 73.47 4.27 6.7 3.22 1 1 .16 PMON68537 GM. _A36318 30.57 10.54 14.83 5.55 36.92 PMON68537 GM _A36319 80 3.65 5.83 2.31 7.02 PMON68537 GM. _A36319 79.89 3.65 5.64 2.35 7.26 PMON68537 GM _A36319 79.4 3.59 5.73 1 .76 8.46 PMON68537 GM. _A36319 78 3.87 6.1 1 2.35 8.5 PMON68537 GM _A36319 76.08 4.22 6.5 2.35 9.74 PMON68537 GM _A36319 75.56 3.89 6.41 1 .78 1 .3 PMON68537 GM. _A36319 75.26 4.27 6.47 2.37 10.5 PMON68537 GM _A36319 75.16 4.1 6.48 2.49 10.66 PMON68537 GM. _A36320 81 .27 3.19 5.84 2.4 6.09 PMON68537 GM. _A36320 80.21 3.27 5.18 2.44 7.76 PMON68537 GM _A36320 79.64 3.38 5.5 2.67 7.63 PMON68537 GM _A36320 79.46 3.38 5.82 2.67 7.42 Constructo Event 18: 1 18: 3 16: 0 18: 0 18: 2 PMON68537 GM A36320 78.5 3.59 6.24 2.49 8 PMON68537 GM_ A36320 73.83 3.79 6.72 2.78 1 1 .74 PMON68537 GM_ A36320 73.1 3.95 6.9 2.39 12.48 PMON68537 GM_ A36320 22.99 8.03 12.19 4.81 50.89 PMON68537 GM_ A36324 75.93 3.77 6.58 2.76 9.76 PMON68537 GM_ A36324 75.1 4.05 7.01 2.83 9.8 PMON68537 GM_ A36324 17.83 8.79 12.78 4.1 1 55.49 PMON68537 GM A36324 16.46 8.88 12.84 4.48 56.29 PMON68537 GM A36324 16.35 9.25 13.51 4.17 55.66 PMON68537 GM_ A36324 15.25 8.99 13.73 4.28 56.69 PMON68537 GM A36324 14.16 10.17 13.95 4.1 1 56.58 PMON68537 GM A36324 13.59 9.87 14.61 4.5 56.33 PMON68537 GM_ A36357 80.19 3.03 5.59 3.2 6.62 PMON68537 GM_ A36357 79.78 3.19 5.51 3.24 6.89 PMON68537 GM_ A36357 78.5 3.55 5.75 3.17 7.71 PMON68537 GM_ .A36357 77.48 3.68 5.71 3.55 8.23 PMON68537 GM .A36357 77.28 3.79 5.66 3.48 8.46 PMON68537 GM. .A36357 77.1 3.51 5.43 3.65 8.99 PMON68537 GM_ .A36357 71.9 4.24 6.47 3.67 12.39 PMON68537 GM _A36357 17.66 9.32 13.26 4.21 54.51 PMON68537 GM. _A36359 77.91 3.35 5.67 3.24 8.53 PMON68537 GM _A36359 77.85 3.29 5.42 3.29 8.87 PMON68537 GM. .A36359 76.71 3.65 6.07 3.35 8.95 PMON68537 GM A36359 71 .73 4.01 6.79 3.49 12.68 PMON68537 GM. _A36359 69.32 4.51 6.99 3.66 14.13 PMON68537 GM _A36359 68.63 4.44 6.91 3.76 14.89 PMON68537 GM. A36359 18.87 8.03 13.38 3.86 54.81 PMON68537 GM _A36359 16.81 9.83 13.08 4.68 54.55 PMON68537 GM _A36360 79.34 3.29 5.99 3.15 6.88 PMON68537 GM. _A36360 75.42 3.47 6.47 3.08 10.26 PMON68537 GM _A36360 75.3 3.86 6.69 3.2 9.64 PMON68537 GM. _A36360 74.51 3.8 6.39 3.32 10.67 PMON68537 GM _A36360 21 .49 6.95 13.07 3.92 53.46 PMON68537 GM. _A36360 20.05 7.4 13.09 3.83 54.57 PMON68537 GM. _A36360 16.08 9.14 13.02 4.64 56.03 PMON68537 GM _A36360 15.86 9.07 13.44 4.49 56.04 PMON68537 GM. _A36361 82.13 2.83 5.67 3.13 4.81 PMON68537 GM _A36361 80.99 3.2 5.79 3.01 5.64 PMON68537 GM _A36361 74.39 3.85 6.33 3.5 10.59 PMON68537 GM. _A36361 18.01 8.46 13.18 3.92 55.41 PMON68537 GM. _A36361 17.99 8.1 1 13.05 4.09 55.7 Constructo Event 18: 1 18: 3 16: 0 18: 0 18: 2 PMON68537 GM A36361 17.35 8.31 13.4 4 55.88 PMON68537 GM A36361 16.81 10.2 12.9 4.32 54.87 PMON68537 GM_ A36361 16.55 8.5 13.21 4.22 56.45 PMON68537 GM A36362 78.05 3.89 6.29 2.81 7.76 PMON68537 GM A36362 76.89 3.69 6.32 3.12 8.76 PMON68537 GM A36362 76.1 4 6.57 3.02 9.24 PMON68537 GM_ A36362 76.01 4.08 6.24 3.03 9.48 PMON68537 GM A36362 75.86 3.76 5.68 3.56 9.95 PMON68537 GM A36362 75.79 4.07 6.43 3.15 9.34 PMON68537 GM_ A36362 74.89 4.14 6.63 3.1 1 10.07 PMON68537 GM A36362 17.22 8.8 13.75 3.77 55.54 PMON68537 GM A36363 79.15 3.57 6.2 3.03 6.84 PMON68537 GM A36363 75.69 3.83 7.07 2.73 9.53 PMON68537 GM A36363 73.97 4.22 6.82 3.39 10.33 PMON68537 GM_ A36363 72.53 4.31 6.64 3.7 1 .59 PMON68537 GM A36363 68.42 4.5 7.05 3.95 14.79 PMON68537 GM_ .A36363 18.39 8.7 13.61 4.1 54.28 P ON68537 GM .A36363 17.54 8.87 14.08 4.07 54.56 PMON68537 GM A36363 15.87 9.66 14.56 4.2 54.69 PMON68537 GM .A36365 78.79 3.1 1 5.87 1 .27 9.9 PMON68537 GM A36365 76.76 3.86 5.79 1 .66 10.91 PMON68537 GM. _A36365 75.41 3.49 6.06 1 .83 12.15 PMON68537 GM _A36365 73.57 3.65 6.1 1 1 .5 14.19 PMON68537 GM _A36365 71 .55 3.56 6.62 1 .24 16.08 PMON68537 GM A36365 70.41 4 6.07 2.15 16.33 PMON68537 GM A36365 66.66 3.9 6.84 1 .5 20.21 PMON68537 GM. _A36365 63.96 4.22 7.08 2.27 21 .52 PMON68537 GM _A36366 75.44 4.33 6.49 3.21 9.32 PMON68537 GM. _A36366 74.75 4.21 6.87 2.71 10.33 PMON68537 GM _A36366 74.69 4.65 6.91 3.06 9.65 PMON68537 GM _A36366 73.23 4.89 7.23 2.99 10.52 PMON68537 GM _A36366 72.53 4.76 7.42 3.26 10.85 PMON68537 GM _A36366 67.15 5.05 7.47 3.33 15.87 PMON68537 GM _A36366 65.81 5.6 7.9 3.37 16.09 PMON68537 GM A36366 62.31 6.19 8.71 3.22 18.55 PMON68537 GM. _A36367 80.56 3.3 6.07 2.58 6.34 P ON68537 GM _A36367 77.78 3.58 6.47 2.66 8.45 PMON68537 GM. _A36367 77.78 3.46 6.25 2.84 8.51 PMON68537 GM. _A36367 77.39 3.81 6.71 2.86 8.1 1 PMON68537 GM _A36367 77.32 3.74 6.17 3.12 8.47 PMON68537 GM _A36367 75.93 3.97 6.23 3.43 9.29 PMON68537 GM _A36367 72.82 4.09 6.85 3.25 1 .88 Constructo Event 18: 1 18: 3 16: 0 18: 0 18: 2 PMON68537 GM_A36367 19.31 7.58 13.7 3.59 55 PMON68537 GM_A36410 21 .67 7.62 13.38 3.43 53.1 PMON68537 GM_A36410 20.9 8.33 12.93 3.64 53.33 PMON68537 GM_A36410 20.21 8.04 13.28 3.86 53.66 PMON68537 GM_A36 10 20.02 8.71 12.79 3.71 53.87 PMON68537 GM_A36410 18.96 8.95 13.3 3.77 54.15 PMON68537 GM_A36410 18.18 8.98 13.56 3.74 54.66 PMON68537 GM_A36410 17.61 9.29 12.93 4.12 55.13 PMON68537 GM_A36410 16.78 9.8 13.78 3.92 54.83 PMON68537 GM_A3641 1 75.06 4.33 6.49 2.93 10.08 PMON68537 GM_A3641 1 74.32 4.46 6.76 2.96 10.38 PMON68537 GM_A3641 1 73.41 4.76 6.91 3.1 1 10.78 PMON68537 GM_A3641 1 73.24 4.87 7.28 2.89 10.67 PMON68537 GM_A36 1 1 22.38 8.17 13.47 3.6 51 .51 PMON68537 GM_A3641 1 18.26 9.07 14.14 3.81 54.02 PMON68537 GM_A3641 1 17.52 10.1 13.1 4.03 54.36 PMON68537 GM_A3641 1 7.02 9.71 13.45 4.02 54.89 A3244 A3244 18.29 7.79 13.69 4.15 55.08 A3244 A3244 17.54 8.19 13.32 4.32 55.57 A3244 A3244 17.13 8.13 13.21 4.46 56.04 A3244 A3244 15.47 9.56 13.04 4.43 56.46 A3244 A3244 15.17 8.95 13.79 4.3 56.78 A3244 A3244 15.05 9.03 14.16 4.01 56.8 A3244 A3244 13.51 10.07 12.95 5.07 57.3 A3244 A3244 13.49 9.91 13.31 4.56 57.67 TABLE 8 Fatty acid composition of simple seeds R1 of events P ON68539 Constructo Event 16: 0 18: 0 18: 1 18: 2 18: 3 PMON68539 GM_A36448 4.51 2.65 79.64 8.66 3.55 PMON68539 GM_A36448 4.62 2.64 78.35 9.99 3.77 PMON68539 GM_A36448 5.89 2.65 76.86 9.79 3.84 PMON68539 GM_A36448 4.92 2.62 72.61 14.61 4.01 PMON68539 GM A36448 5.48 2.86 71 .07 15.63 4.16 PMON68539 GM_A36448 13.5 4.2 16.28 56.86 8.29 PMON68539 GM_A36448 14.49 4.67 14.88 56.56 9.07 PMON68539 GM_A36449 5.16 2.42 81 .91 6.54 3.12 PMON68539 GM_A36449 4.26 2.41 79.99 8.4 3.94 PMON68539 GM_A36449 4.26 2.72 79.07 9.32 3.38 PMON68539 GM_A36449 5.01 2.54 75.71 1 1 .94 3.9 PMON68539 GM_A36449 4.34 2.76 75.07 12.75 4.16 PMON68539 GM_A36449 1 1.57 3.52 44.08 35.22 4.98 PMON68539 GM_A36449 13.42 3.84 21 .35 52.38 8.17 PMON68539 GM_A36449 13.25 3.99 15.3 57.6 9.04 PMON68539 GM_A36450 3.28 2.6 82.21 7.26 3.95 PMON68539 GM_A36450 4.16 2.51 80.93 7.72 3.76 PMON68539 GM_A36450 4.3 3.42 78.78 8.43 4.22 PMON68539 GM_A36450 4.84 3.16 77.07 9.6 4.22 PMON68539 GM_A36450 5.1 1 3.1 75.21 10.98 4.49 PMON68539 GM_A36450 13.74 4.26 17.31 54.32 10.1 1 PMON68539 GM_A36450 13.82 4.34 17.13 54.96 9.47 PMON68539 GM_A36450 13.56 3.83 17.06 56.7 8.6 PMON68539 GM_A36705 9.73 1 .83 75.04 8.23 4.27 PMON68539 GM_A36705 10.85 1.74 72.89 9.29 4.53 PMON68539 GM_A36705 10.05 1 .78 72.68 9.83 4.48 PMON68539 GM_A36705 10.02 1 .77 72.57 10.04 4.36 PMON68539 G _A36705 10.75 1.75 72.37 9.68 4.77 PMON68539 GM_A36705 10.58 1 .78 70.35 1 1 .64 4.43 PMON68539 GM_A36705 7.69 5.63 16.21 60.39 8.85 PMON68539 GM_A36705 8.02 5.69 15.2 5.02 19.83 49.96 8.86 A3244 12.63 4.84 19.55 53.18 8.66 A3244 13.27 4.48 8.28 54.4 8.5 A3244 13.22 4.91 17.38 54.73 8.63 A3244 13.44 4.81 15.46 56.49 8.91 EXAMPLE 11 A construct pMON95829 as described in the 3D example is used to introduce an intron double-stranded RNA formation construct FAD2-1 into the soybean seed to suppress the Fad2 gene. The vector is stably introduced into soybeans (variety Asgrow A4922) via a strain Agrobacterium tumefaciens ABI (Martinell, U.S. Patent No. 6,384,301). The CP4 selectable marker allows the transformed soybean plants to be identified by selection in glyphosate herbicide-containing medium. Subsequently, the genomes of transformed plants are classified for concurrent tandem insertion of the first T-DNA and the second T-DNA, that is, in the assembly "right edge to right edge". The classification is carried out with Southern hybridization mapping methods. Transformed soybean plants that contain the preferred configuration in their genome are transferred to a greenhouse for seed production. For example, leaf tissue is taken from the R0 plants transformed with construct pMON95829 and Southern analysis is performed. Restriction enzyme probes and digestions are selected in order to identify events that contain a right edge-right border assembly ("RB-RB") of both T-DNA. Typically, approximately 25% of all transformants have T-DNA RB-RB properly assembled. The fatty acid compositions are analyzed from the seed of Soybean lines transformed with a construct pMON95829 using gas chromatography as described in Example 4 to identify methyl esters of fatty acid compounds extracted from seeds. First, six Ri seeds taken from soybean plants transformed with construct pMON95829 are harvested, and the fatty acid composition of each single seed is determined. Since the Ri plants of each event are segregated for the transgenes and, therefore, produce seeds with conventional soybean seed composition, as well as modified versions. Positive seeds are grouped and averaged for each event. Pooled positive averages demonstrate that the mono- and polyunsaturated fatty acid compositions are altered in the oil of the seeds of transgenic soybean lines compared to those of the non-transformed soybean seed (see Table 9). For example, the suppression of FAD2 provides plants with increased amount of oleic acid ester compounds.

TABLE 9 Fatty Acid Composition of Single Seeds R1 of Events PMON95829 EXAMPLE 12 A pMON93505 construct as described in the 3D example is used to introduce an intron double-stranded RNA formation construct FAD2-1 into the soybean seed to suppress the Fad2 gene. The vector is stably introduced into soybeans (variety Asgrow A4922) via a strain Agrobacterium tumefaciens ABI (Martinell, U.S. Patent No. 6,384,301). The selectable CP4 marker allows transformed soybean plants to be identified by selection in medium containing glyphosate herbicide. Subsequently, the genomes of transformed plants are classify for concurrent tandem insertion of the first T-DNA and the second T-DNA, that is, in the assembly "right edge to right edge". The classification is carried out with Southern hybridization mapping methods. Transformed soybean plants that contain the preferred configuration in their genome are transferred to a greenhouse for seed production. For example, leaf tissue is taken from the R0 plants transformed with construct pMON93505 and Southern analysis is performed. Restriction enzyme probes and digestions are selected in order to identify events that contain a right edge-right border assembly ("RB-RB") of both T-DNA. Typically, approximately 25% of all transformants have T-DNA RB-RB properly assembled. The fatty acid compositions are analyzed from the seed of soy lines transformed with a construct pMON93505 using gas chromatography as described in example 4 to identify methyl esters of fatty acid compounds extracted from seeds. First, six seeds taken from soybean plants transformed with construct pMON93505 are harvested, and the fatty acid composition of each single seed is determined. Since the Ri plants of each event are segregated for the transgenes and, therefore, produce seeds with conventional soybean seed composition, as well as modified versions. Positive seeds are grouped and averaged for each event. The pooled positive averages show that the mono- and polyunsaturated fatty acid compositions are altered in the oil of the seeds of transgenic soybean lines compared to those of the non-transformed soybeans (see table 10). By example, the deletion of FAD2 provides plants with increased amount of oleic acid ester compounds.

TABLE 10 Fatty acid composition of simple seeds R1 of events PMON93505 Constructo Event # 16: 0 18: 0 18: 2 18: 3 PMON93505 GM_A87814 1.3 1.0 84.9 5.5 6.3 PMON93505 GM_A86449 1.5 0.9 84.9 4.9 6.8 PMON93505 GM_A86032 1.5 1.1 83.5 6.3 7.0 PMON93505 GM_A86159 1.5 0.9 82.8 6.7 7.5 PMON93505 GM_A86178 1.7 1.0 82.5 6.7 7.3 PMON93505 GM_A86075 1.4 0.9 81.4 6.6 8.5 PMON93505 GM_A86303 1.0 0.6 81.4 7.4 8.8 PMON93505 GM_A86454 1.4 0.9 79.9 7.4 8.8 PMON93505 GM_A86799 1.4 1.1 79.4 9.6 7.7 PMON93505 GM_A85997 2.2 2.5 79.3 7.7 7.4 PMON93505 GM_A86058 1.8 1.0 76.8 11.3 8.3 PMON93505 GM_A86274 1.2 0.7 74.6 10.2 11.9 PMON93505 GM_A86325 1.1 0.7 72.8 15.4 9.2 PMON93505 GM_A85969 2.0 0.7 70.7 13.6 12.1 PMON93505 GM_A86033 1.7 0.9 69.1 18.2 9.5 PMON93505 GM_A86372 1.7 1.0 65.7 12.6 17.6 PMON93505 GM_A86403 1.5 0.9 64.6 16.8 15.4 PMON93505 GM_A87803 1.1 0.6 57.7 26.0 13.8 PMON93505 GM_A86036 3.1 1.5 54.8 30.4 9.7 PMON93505 GM_A86269 4.9 1.8 51.4 31.9 9.5 EXAMPLE 13 A pMON93506 construct as described in the 3D example is used to introduce an intron double-stranded RNA formation construct FAD2-1 into the soybean seed to suppress the FAD2 gene. The vector is stably introduced into soybeans (variety Asgrow A4922) via a strain Agrobacterium tumefaciens ABI (Martinell, U.S. Patent No. 6,384,301). The selectable CP4 marker allows transformed soybean plants to be identified by selection in medium containing glyphosate herbicide. Subsequently, the genomes of transformed plants are classified for concurrent tandem insertion of the first T-DNA and the second T-DNA, that is, in the assembly "right edge to right edge". The classification is carried out with Southern hybridization mapping methods. Transformed soybean plants that contain the preferred configuration in their genome are transferred to a greenhouse for seed production. For example, leaf tissue is taken from the R0 plants transformed with pMON93506 construct and Southern analysis is performed. Restriction enzyme probes and digestions are selected in order to identify events that contain a right edge-right border assembly ("RB-RB") of both T-DNA. Typically, approximately 25% of all transformants have T-DNA RB-RB properly assembled. The fatty acid compositions are analyzed from the seed of Soybean lines transformed with a pMON93506 construct using gas chromatography as described in example 4 to identify Methyl esters of fatty acid compounds extracted from seeds.

First, six Ri seeds taken from soybean plants transformed with construct pMON93506 are collected, and the composition of Fatty acid from each single seed is determined. Since the P plants of each event are segregated for the transgenes and, therefore, produce seeds with conventional soybean seed composition, as well as modified versions. Positive seeds are grouped and averaged to each event The pooled positive averages show that the mono- and polyunsaturated fatty acid compositions are altered in the oil of the seeds of transgenic soybean lines compared to those of non-transformed soybeans (see table 11). By example, the deletion of FAD2 provides plants with increased amount of oleic acid ester compounds.

TABLE 1 1 Fatty acid composition of simple seeds R1 of events PMON93506 Constructo Event # 16: 0 18: 0 18: 1 18: 2 18: 3 PMON93506 GM_A87174 2.2 0.8 88.1 2.3 5.1 PMON93506 GM_A86998 2.1 0.6 87.1 3.4 5.5 Constructo Event # 16: 0 18: 0 18: 1 18: 2 18: 3 PMON93506 GM_A87075 2.7 1 .2 85.9 4.8 4.2 PMON93506 GM_A87255 2.9 0.8 84.8 5.5 5.4 PMON93506 GM_A91253 2.7 0.9 84.5 5.9 5.1 PMON93506 GM_A86561 2.8 0.7 83.8 5.9 6.0 PMON93506 GM_A86875 3.1 1 .0 83.6 6.2 5.5 PMON93506 GM_A89967 1 .8 1 .3 83.2 4.1 7.9 PMON93506 GM_A86927 2.1 0.8 82.6 4.8 8.5 PMON93506 GM_A87883 2.7 0.7 82.4 6.5 7.2 PMON93506 GM_A87133 3.0 3.1 81.5 5.2 6.3 PMON93506 GM_A88072 2.8 0.7 80.6 8.2 7.1 PMON93506 GM_A87069 3.8 0.7 80.4 8.2 6.4 PMON93506 GM_A86835 2.7 3.0 80.3 6.4 6.4 PMON93506 GM_A87929 2.7 1 .0 76.3 7.8 1 1 .5 PMON93506 GM "A87298 3.0 1 .2 72.9 13.0 9.1 PMON93506 GM_A91226 3.4 1 .0 69.3 18.0 7.7 PMON93506 GM_A88076 3.7 3.9 68.0 15.4 8.1 PMON93506 GM_A86530 2.9 1 .0 59.3 25.0 1 1 .5 PMON93506 GM_A87292 4.6 4.3 54.2 27.6 8.3 P ON93506 GM_A87076 5.5 0.9 46.7 38.0 8.4 EXAMPLE 14 A construct pMON93501 as described in example 3B is used to introduce a double-stranded RNA formation construct FAD2-1 intron in the soybean to suppress the FAD2 gene. The vector is Stably introduced into soybeans (variety Asgrow A4922) via a strain Agrobacterium tumefaciens ABI (Martinell, Patent of E.U.A. No. 6,384,301). The selectable CP4 marker allows seed plants processed soybeans are identified by the selection in the medium that contains glyphosate herbicide.

The fatty acid compositions are analyzed from the seed of Soybean lines transformed with a pMON93501 construct using gas chromatography as described in example 4 to identify Methyl esters of fatty acid compounds extracted from seeds.

First, six Ri seeds taken from soybean plants transformed with construct pMON93501 are collected, and the composition of Fatty acid from each single seed is determined. Since the RT plants of each event are segregated for the transgenes and, therefore, produce seeds with conventional soybean seed composition, as well as modified versions. Positive seeds are grouped and averaged to each event The pooled positive averages show that the mono- and polyunsaturated fatty acid compositions are altered in the oil of the seeds of transgenic soybean lines compared to those of the non-transformed soybean seed (see table 12). By example, the deletion of FAD2 provides plants with increased amount of oleic acid ester compounds.

TABLE 12 Fatty acid composition of simple seeds R1 of events PMON93501 Constructo Event # 16: 0 18: 0 18: 1 18: 2 18: 3 PMON93501 GM_A85435 4.4 1 .1 85.8 2.5 5.1 PMON93501 GM_A85439 4.6 0.9 84.8 3.7 5.1 Constructo Event # 16: 0 18: 0 18: 1 18: 2 18: 3 PMON93501 GM_A85276 4.8 1 .4 84.3 3.0 4.9 PMON93501 GM_A85697 4.8 1 .3 83.6 3.8 5.6 PMON93501 GM_A85777 6.6 1.8 80.0 4.5 6.4 PMON93501 GM_A84790 7.2 5.7 78.3 2.9 4.7 PMON93501 GM_A85910 4.2 1.1 77.8 6.9 9.3 PMON93501 GM_A86186 5.3 1 .1 77.4 7.4 7.7 PMON93501 GM_A85065 7.3 2.2 76.8 5.7 6.9 PMON93501 GM_A85744 4.1 0.9 76.0 7.4 10.6 PMON93501 GM_A85261 4.7 1 .0 75.8 4.9 1 1 .9 PMON93501 GM_A85479 3.7 1 .1 75.8 8.6 9.8 PMON93501 GM_A85819 4.5 1 .7 74.9 6.9 1 1 .1 PMON93501 GM_A85945 4.6 1 .2 74.6 8.7 10.0 PMON93501 GM_A85301 6.9 1 .2 73.1 9.5 8.4 PMON93501 GM_A85929 6.1 1 .4 72.4 10.8 8.7 PMON93501 GM_A85908 6.9 1.3 70.0 8.0 13.6 PMON93501 GM_A85393 4.8 1 .3 67.0 13.3 12.2 PMON93501 GM_A85756 4.8 1 .8 57.3 17.6 17.8 PMON93501 GM_A85415 5.0 1 .3 52.9 26.0 12.1 PMON93501 GM_A85950 5.5 1 .8 47.5 38.6 6.1 PMON93501 GM_A84705 5.7 2.3 46.0 37.7 7.4 PMON93501 GM_A85787 4.5 1 .6 43.4 37.0 13.1 EXAMPLE 15 A construct pMON97552 as described in the 2D example is used to introduce a double-stranded RNA formation construct FAD2-1 intron in the soybean to suppress the FAD2 gene. The vector is Stably introduced into soybeans (variety Asgrow A4922) via a strain Agrobacterium tumefaciens ABI (Martinell, Patent of E.U.A. No. 6,384,301). The selectable CP4 marker allows seed plants processed soybeans are identified by the selection in the medium that contains glyphosate herbicide. The fatty acid compositions are analyzed from the seed of soy lines transformed with a pMON97552 construct using gas chromatography as described in Example 4 to identify methyl esters of fatty acid compounds extracted from seeds. First, six Ri seeds taken from soybean plants transformed with construct pMON97552 are harvested, and the fatty acid composition of each single seed is determined. Since the plants of each event are segregated for the transgenes and, therefore, they produce seeds with conventional soybean seed composition, as well as modified versions. Positive seeds are grouped and averaged for each event. The pooled positive averages demonstrate that the mono- and polyunsaturated fatty acid compositions are altered in the oil of the seeds of transgenic soybean lines as compared to those of the non-transformed soybean seed (see Table 13). For example, the suppression of FAD2 provides plants with increased amount of oleic acid ester compounds.

TABLE 13 Fatty Acid Composition of Single Seeds R1 of Events PMON97552 EXAMPLE 16 A pMON93758 construct as described in example 2D is used to introduce an intron double-stranded RNA formation construct FAD2-1 into the soybean seed to suppress the FAD2 gene. The vector is stably introduced into soybeans (variety Asgrow A4922) via a strain Agrobacterium tumefaciens ABI (Martinell, U.S. Patent No. 6,384,301). The selectable CP4 marker allows transformed soybean plants to be identified by selection in medium containing glyphosate herbicide. The fatty acid compositions are analyzed from the seed of soy lines transformed with a construct pMON93758 using gas chromatography as described in example 4 to identify methyl esters of fatty acid compounds extracted from seeds. First, six seeds taken from soybean plants transformed with construct pMON93758 are harvested, and the composition of Fatty acid from each single seed is determined. Since the Fd plants of each event are segregated for the transgenes and, therefore, produce seeds with conventional soybean seed composition, as well as modified versions. Positive seeds are grouped and averaged to each event The pooled positive averages show that the mono- and polyunsaturated fatty acid compositions are altered in the oil of the seeds of transgenic soybean lines compared to those of the non-transformed soybeans (see table 14). By example, the deletion of FAD2 provides plants with increased amount of oleic acid ester compounds.

TABLE 14 Fatty acid composition of simple seeds R1 of events P ON93758 Constructo Event # 16: 0 18: 0 18: 2 18: 3 PMON93758 GM_A89686 2.7 2.9 82.7 5.3 5.5 PMON93758 GM_A89678 2.9 2.9 81 .8 5.5 6.0 PMON93758 GM_A89670 2.8 3.0 81 .7 5.6 6.1 PMON93758 GM_A89688 2.7 3.2 81 .6 5.8 5.9 PMON93758 GM_A89683 2.9 2.9 81.5 5.8 6.1 PMON93758 GM A89699 2.7 3.1 81 .4 5.8 6.1 PMON93758 GM_A89675 2.9 3.0 81 .4 5.6 6.2 PMON93758 GM_A89690 3.0 2.8 81 .3 5.7 6.3 PMON93758 GM_A89680 3.0 2.8 81 .3 5.9 6.0 PMON93758 GM_A89674 2.9 2.9 80.4 6.3 6.7 PMON93758 GM_A89677 3.0 2.8 79.7 7.0 6.8 PMON93758 GM_A89676 3.0 2.9 78.7 7.6 7.4 PMON93758 GM_A89694 3.2 2.8 76.7 8.8 8.0 PMON93758 GM_A89696 3.0 2.6 74.7 10.4 8.9 EXAMPLE 17 A pMON97553 construct as described in Example 2D is used to introduce an intron double-stranded RNA formation construct FAD2-1 into the soybean seed to suppress the FAD2 gene. The vector is stably introduced into soybeans (variety Asgrow A4922) via a strain Agrobacterium tumefaciens ABI (Martinell, U.S. Patent No. 6,384,301). The CP4 selectable marker allows the transformed soybean plants to be identified by selection in glyphosate herbicide-containing medium. The fatty acid compositions are analyzed from the seed of soybean lines transformed with a pMON97553 construct using gas chromatography as described in Example 4 to identify methyl esters of fatty acid compounds extracted from seeds. First, six seeds taken from soybean plants transformed with construct pMON97553 are harvested, and the fatty acid composition of each single seed is determined. Since the Ri plants of each event are segregated for the transgenes and, therefore, produce seeds with conventional soybean seed composition, as well as modified versions. Positive seeds are grouped and averaged for each event. The pooled positive averages show that the mono- and polyunsaturated fatty acid compositions are altered in the oil of the seeds of transgenic soybean lines compared to those of the non-transformed soybeans (see table 15). By example, the deletion of FAD2 provides plants with increased amount of oleic acid ester compounds.

TABLE 15 Fatty acid composition of simple seeds R1 of events PMON97553 Constructo Event # 16: 0 18: 0 18: 2 18: 3 PMON97553 GM_A98670 2.1 2.6 86.7 2.9 4.3 PMON97553 GM_A98595 2.3 2.7 86.3 3.5 4.7 PMON97553 GM_A98649 2.2 2.9 86.3 3.6 4.7 PMON97553 GM_A98669 2.1 3.0 85.5 3.3 4.6 PMON97553 GM_A98656 2.4 2.8 85.5 4.2 4.6 PMON97553 GM_A98643 2.3 2.8 85.0 3.8 4.9 PMON97553 GM_A98647 2.2 2.8 84.2 5.1 5.6 PMON97553 GM_A98582 2.6 2.8 84.0 4.1 5.6 PMON97553 GM_A98674 2.1 2.3 83.9 5.8 5.3 PMON97553 GM_A98663 2.2 2.8 83.3 5.5 5.1 PMON97553 GM_A98587 2.8 2.8 83.0 5.5 5.3 PMON97553 GM_A98592 2.9 2.9 82.9 4.6 5.8 PMON97553 GM_A98677 2.2 3.0 82.4 5.9 5.4 PMON97553 GM_A98594 2.2 2.9 82.3 6.5 5.4 PMON97553 GM_A98659 2.5 3.0 82.2 5.4 6.1 PMON97553 GM_A98622 2.8 3.0 81.6 6.0 6.1 PMON97553 GM_A98589 2.9 3.0 81.3 6.2 6.1 PMON97553 GM_A98679 2.2 3.1 81.2 6.7 5.7 PMON97553 GM_A98642 2.3 3.1 80.0 7.4 6.1 PMON97553 GM_A98639 2.7 3.0 78.4 8.0 6.8 PMON97553 GM_A98563 3.3 2.9 78.1 9.9 5.6 PMON97553 GM_A98618 2.9 2.8 78.0 8.8 6.9 PMON97553 GM_A98567 2.7 3.2 77 .5 9.1 6.3 PMON97553 GM_A98625 2.3 2.9 77.4 9.5 6.9 PMON97553 GM_A98660 3.3 2.9 77.1 10.7 5.6 PMON97553 GM_A98615 2.7 3.2 76.4 9.9 7.1 Constructo Event # 16: 0 18: 0 18: 2 18: 2 PMON97553 GM_A98561 3.3 3.1 75.3 10.9 6.7 PMON97553 GM_A98603 2.9 3.6 73.5 1 1 .0 7.8 PMON97553 GM_A98648 2.7 3.3 70.2 14.4 8.3 PMON97553 GM_A98565 3.2 2.8 67.9 17.9 7.2 PMON97553 GM_A98681 3.1 3.0 65.9 19.3 7.7 EXAMPLE 18 A pMON93770 construct as described in the 2D example is used to introduce a double-stranded RNA formation construct FAD2-1 intron in the soybean to suppress the FAD2 gene. The vector is Stably introduced into soybeans (variety Asgrow A4922) via a strain Agrobacterium tumefaciens ABI (Martinell, U.S. Patent No. 6,384,301). The CP4 selectable marker allows seed plants processed soybeans are identified by the selection in the medium that contains glyphosate herbicide.

The fatty acid compositions are analyzed from the seed of Soybean lines transformed with a pMON93770 construct using gas chromatography as described in example 4 to identify Methyl esters of fatty acid compounds extracted from seeds.

First, six Ri seeds taken from soybean plants transformed with construct pMON93770 are collected, and the composition of Fatty acid from each single seed is determined. Since the plants R-de each event are segregated for the transgenes and, therefore, produce seeds with conventional soybean seed composition, as well as modified versions. Positive seeds are grouped and averaged for each event. The pooled positive averages demonstrate that the mono- and polyunsaturated fatty acid compositions are altered in the oil of the seeds of transgenic soybean lines compared to those of the non-transformed soybean seed (see Table 16). For example, the suppression of FAD2 provides plants with increased amount of oleic acid ester compounds.

TABLE 16 Fatty Acid Composition of Single Seeds R1 of Events PMON93770 EXAMPLE 19 A pMON93759 construct as described in example 2D is used to introduce an intron double-stranded RNA formation construct FAD2-1 into the soybean seed to suppress the FAD2 gene. The vector is Stably introduced into soybeans (variety Asgrow A4922) via a strain Agrobacterium tumefaciens ABI (Martinell, U.S. Patent No. 6,384,301). The CP4 selectable marker allows the transformed soybean plants to be identified by selection in glyphosate herbicide-containing medium. The fatty acid compositions are analyzed from the seed of soy lines transformed with a pMON93759 construct using gas chromatography as described in example 4 to identify methyl esters of fatty acid compounds extracted from seeds. First, six Ri seeds taken from soybean plants transformed with construct pMON93759 are harvested, and the fatty acid composition of each single seed is determined. Since the Ri plants of each event are segregated for the transgenes and, therefore, produce seeds with conventional soybean seed composition, as well as modified versions. Positive seeds are grouped and averaged for each event. The pooled positive averages demonstrate that the mono- and polyunsaturated fatty acid compositions are altered in the oil of the seeds of transgenic soybean lines in comparison with those of the non-transformed soybean seed (see Table 17). For example, the suppression of FAD2 provides plants with increased amount of oleic acid ester compounds.

TABLE 17 Fatty Acid Composition of Single Seeds R1 of Events PMON93759 EXAMPLE 20 A pMON97554 construct as described in Example 2D is used to introduce an FAD2-1 double stranded RNA-forming construct into the soybean seed to suppress the FAD2 gene. The vector is stably introduced into soybeans (Asgrow variety A4922) via a strain Agrobacterium tumefaciens ABI (Martinell, U.S. Patent No. 6,384,301). The CP4 selectable marker allows the transformed soybean plants to be identified by selection in glyphosate herbicide-containing medium. The fatty acid compositions are analyzed from the seed of soybean lines transformed with a pMON97554 construct using gas chromatography as described in example 4 to identify methyl esters of fatty acid compounds extracted from seeds. First, six P seeds taken from soybean plants transformed with construct pMON97554 are harvested, and the fatty acid composition of each single seed is determined. Since the Ri plants of each event are segregated for the transgenes and, therefore, produce seeds with conventional soybean seed composition, as well as modified versions. Positive seeds are grouped and averaged for each event. The pooled positive averages demonstrate that the mono- and polyunsaturated fatty acid compositions are altered in the oil of the seeds of transgenic soybean lines as compared to those of the non-transformed soybean seed (see Table 18). For example, the suppression of FAD2 provides plants with increased amount of oleic acid ester compounds.

TABLE 18 Single-ingredient fatty acid composition R1 of events PMON97554 EXAMPLE 21 A pMON93771 construct as described in Example 2D is used to introduce an intron double-stranded RNA formation construct FAD2-1 into the soybean seed to suppress the FAD2 gene. The vector is stably introduced into soybeans (variety Asgrow A4922) via a strain Agrobacterium tumefaciens ABI (Martinell, U.S. Patent No. 6,384,301). The selectable CP4 marker allows transformed soybean plants to be identified by selection in medium containing glyphosate herbicide. The fatty acid compositions are analyzed from the seed of soybean lines transformed with a construct pMON93771 using gas chromatography as described in example 4 to identify methyl esters of fatty acid compounds extracted from seeds.

First, six Ri seeds taken from soybean plants transformed with construct pMON93771 are collected, and the composition of Fatty acid from each single seed is determined. Since the plants F \ ^ of each event are segregated for the transgenes and, therefore, produce seeds with conventional soybean seed composition, as well as modified versions. Positive seeds are grouped and averaged to each event The pooled positive averages show that the mono- and polyunsaturated fatty acid compositions are altered in the oil of the seeds of transgenic soybean lines compared to those of the non-transformed soybean seed (see table 19). By example, the deletion of FAD2 provides plants with increased amount of oleic acid ester compounds.

TABLE 19 Fatty acid composition of simple seeds R1 of events PMON93771 Constructo Event # 16: 0 18: 0 18: 1 18: 2 18: 3 PMON93771 GM_A97841 2.5 2.3 70.8 17.0 6.6 PMON93771 GM_A97839 3.8 3.0 65.8 18.3 8.1 PMON93771 GM_A97836 4.1 2.9 65.5 19.3 7.1 PMON93771 GM_A97844 2.6 2.7 65.2 20.9 8.0 PMON93771 GM_A97835 4.4 2.9 62.9 21 .0 7.8 PMON93771 GM_A97852 3.3 3.1 62.9 21 .0 8.9 PMON93771 GM_A97857 3.4 2.7 61 .7 22.6 8.7 PMON93771 GM_A97846 4.2 2.7 52.0 30.8 9.6 EJ EMPLO 22 A pMON97555 construct as described in example 2D is used to introduce an intron double-stranded RNA formation construct FAD2-1 into the soybean seed to suppress the FAD2 gene. The vector is stably introduced into soybeans (variety Asgrow A4922) via a strain Agrobacterium tumefaciens ABI (Martinell, U.S. Patent No. 6,384,301). The selectable CP4 marker allows transformed soybean plants to be identified by selection in medium containing glyphosate herbicide. The fatty acid compositions are analyzed from the seed of soybean lines transformed with a pMON97555 construct using gas chromatography as described in example 4 to identify methyl esters of fatty acid compounds extracted from seeds. First, six P seeds taken from soybean plants transformed with construct pMON97555 are harvested, and the fatty acid composition of each single seed is determined. Since the Ri plants of each event are segregated for the transgenes and, therefore, produce seeds with conventional soybean seed composition, as well as modified versions. Positive seeds are grouped and averaged for each event. The pooled positive averages show that the mono- and polyunsaturated fatty acid compositions are altered in the oil of the seeds of transgenic soybean lines compared to those of the non-transformed soybean seed (see table 20). For example, the suppression of FAD2 provides plants with increased amount of oleic acid ester compounds.

TABLE 20 Fatty acid composition of single seeds R1 of events P ON97555 EXAMPLE 23 A pMON93760 construct as described in Example 2D is used to introduce an intron double-stranded RNA formation construct FAD2-1 into the soybean seed to suppress the FAD2 gene. The vector is stably introduced into soybeans (variety Asgrow A4922) via a strain Agrobacterium tumefaciens ABI (Martinell, U.S. Patent No. 6,384,301). The selectable CP4 marker allows transformed soybean plants to be identified by selection in glyphosate herbicide-containing medium. The fatty acid compositions are analyzed from the seed of soybean lines transformed with a pMON93760 construct using gas chromatography as described in Example 4 to identify methyl esters of fatty acid compounds extracted from seeds. First, six P seeds taken from soybean plants transformed with construct pMON93760 are harvested, and the fatty acid composition of each single seed is determined. Since the Ri plants of each event are segregated for the transgenes and, therefore, produce seeds with conventional soybean seed composition, as well as modified versions. Positive seeds are grouped and averaged for each event. The pooled positive averages demonstrate that the mono- and polyunsaturated fatty acid compositions are altered in the oil of the seeds of transgenic soybean lines as compared to those of the non-transformed soybean seed (see Table 21). For example, intron FAD2-1, reduced in length by 320 contiguous nucleotides of the 5 'end (SEQ ID NO: 1) and capable of forming dsRNA, at least partially suppresses FAD2.

TABLE 21 Fatty Acid Composition of Single Seeds R1 of Events PMON93760 EXAMPLE 24 A pMON93772 construct as described in example 2D is used to introduce an intron double-stranded RNA formation construct FAD2-1 into the soybean seed to suppress the FAD2 gene. The vector is stably introduced into soybeans (variety Asgrow A4922) via a strain Agrobacterium tumefaciens ABI (Martinell, U.S. Patent No. 6,384,301). The selectable CP4 marker allows transformed soybean plants to be identified by selection in medium containing glyphosate herbicide.

The fatty acid compositions are analyzed from the seed of soy lines transformed with a pMON93772 construct using gas chromatography as described in example 4 to identify methyl esters of fatty acid compounds extracted from seeds. First, six Ri seeds taken from soybean plants transformed with construct pMON93772 are harvested, and the fatty acid composition of each single seed is determined. Since the Ri plants of each event are segregated for the transgenes and, therefore, produce seeds with conventional soybean seed composition, as well as modified versions. Positive seeds are grouped and averaged for each event. The pooled positive averages demonstrate that the mono- and polyunsaturated fatty acid compositions are altered in the oil of the seeds of transgenic soybean lines as compared to those of the non-transformed soybean seed (see Table 22). For example, intron FAD2-1, reduced in length by 360 contiguous nucleotides of the 3 'end (SEQ ID NO: 1) and capable of forming dsRNA, at least partially suppresses FAD2 for some events.

TABLE 22 Fatty acid composition of single seeds R1 of events PMON93772 EXAMPLE 25 A pMON97556 construct as described in example 2D is used to introduce an intron double-stranded RNA formation construct FAD2-1 into the soybean seed to suppress the FAD2 gene. The vector is stably introduced into soybeans (variety Asgrow A4922) via a strain Agrobacterium tumefaciens ABI (Martinell, U.S. Patent No. 6,384,301). The selectable CP4 marker allows transformed soybean plants to be identified by selection in medium containing glyphosate herbicide. The fatty acid compositions are analyzed from the seed of soybean lines transformed with a pMON97556 construct using gas chromatography as described in Example 4 to identify methyl esters of fatty acid compounds extracted from seeds. First, six seeds taken from soybean plants transformed with construct pMON97556 are harvested, and the fatty acid composition of each single seed is determined. Since the Ri plants of each event are segregated for the transgenes and, therefore, produce seeds with conventional soybean seed composition, as well as modified versions. Positive seeds are grouped and averaged for each event. The pooled positive averages demonstrate that the mono- and polyunsaturated fatty acid compositions are altered in the oil of the seeds of transgenic soybean lines compared to those of the non-transformed soybean seed (see Table 23). For example, intron FAD2-1, reduced in length by 200 contiguous nucleotides of the 3 'end (SEQ ID NO: 1) and capable of forming dsRNA, at least partially suppresses FAD2.

TABLE 23 Fatty acid composition of single seeds R1 of events PMON97556 EXAMPLE 26 A pMON93764 construct as described in Example 2D is used to introduce an intron double-stranded RNA formation construct FAD2-1 into the soybean seed to suppress the FAD2 gene. The vector is stably introduced into soybeans (variety Asgrow A4922) via a strain Agrobacterium tumefaciens ABI (Martinell, U.S. Patent No. 6,384,301). The selectable CP4 marker allows transformed soybean plants to be identified by selection in medium containing glyphosate herbicide. The fatty acid compositions are analyzed from the seed of soy lines transformed with a pMON93764 construct using gas chromatography as described in example 4 to identify Methyl esters of fatty acid compounds extracted from seeds.

First, six Ri seeds taken from soybean plants transformed with construct pMON93764 are harvested, and the composition of Fatty acid from each single seed is determined. Since the plants B ^ of each event are segregated for the transgenes and, therefore, produce seeds with conventional soybean seed composition, as well as modified versions. Positive seeds are grouped and averaged to each event The pooled positive averages show that the mono- and polyunsaturated fatty acid compositions are altered in the oil of the seeds of transgenic soybean lines compared to those of the non-transformed soybean seed (see table 24). As well, FAD2-1 intron, reduced in length by 400 contiguous end nucleotides 3 '(SEQ ID NO: 1) and capable of forming dsRNA, does not substantially reduce the expression of FAD2.

TABLE 24 Fatty acid composition of simple seeds R1 of events PMON93764 Constructo Event # 16: 0 18: 0 18: 1 18: 2 PMON93764 GM_A98489 2.1 2.2 28.1 60.5 6.5 PMON93764 GM_A98452 2.2 2.2 27.4 61 .3 6.8 PMON93764 GM_A98451 2.3 2.5 26.2 60.7 7.8 P ON93764 GM_A98467 2.5 2.8 25.4 60.9 8.2 Constructo Event # 16: 0 18: 0 18: 1 18: 2 18: 3 PMON93764 GM_A98455 1 .8 2.3 24.4 63.5 7.8 PMON93764 GM_A98499 1 .8 2.5 24.1 63.5 7.8 PMON93764 GM_A98453 2.5 2.6 23.7 63.2 7.5 PMON93764 GM_A98492 1 .6 2.7 23.7 63.6 7.7 PMON93764 GM_A98456 1 .8 2.4 23.4 64.2 8.0 PMON93764 GM_A98471 2.2 2.7 23.4 64.2 7.4 PMON93764 GM_A98500 2.5 2.3 22.9 64.1 7.9 PMON93764 GM_A98482 2.3 2.5 22.9 64.6 7.3 PMON93764 GM_A98485 2.5 2.7 22.8 63.8 8.0 PMON93764 GM_A98463 1 .9 2.2 22.6 64.7 8.3 PMON93764 GM_A98469 3.4 2.5 22.1 63.3 8.5 PMON93764 GM_A98474 1 .6 2.3 21 .5 65.7 8.4 PMON93764 GM_A98483 2.0 2.5 21 .4 65.4 8.5 PMON93764 GM_A98476 2.7 2.6 21 .2 64.4 8.8 PMON93764 GM_A98498 2.5 2.5 21 .1 64.8 8.9 PMON93764 GM_A98496 2.5 2.3 20.6 65.2 8.9 PMON93764 GM_A98468 1 .9 2.7 19.3 66.0 9.7 EXAMPLE 27 TaqMan is an assay that quantifies nucleic acids via a amplification and fluorescence measurements in real time (also called Real-time PCR). This procedure is used to determine the degree of deletion of target transcript in seeds of transgenic development. For determine the absolute transcript levels of target mRNA in a sample, a standard curve is established for each TaqMan experiment.

For this purpose, different amounts of target gene sequence Cloned soybean, diluted in 20 ng total RNA from canola, are amplified in parallel with samples of unknown target quantities. The accuracy of Transcript copy number determined in this way has a margin of error of 25%. For the tempered material, total RNA is extracted using an ABI 6100 preparative nucleic acid station, and 20 ng a TaqMan sample is used. Samples are analyzed in an ABI 700 sequence detection instrument using one-step ABI Prism master-mix RT-PCR chemistry. The TaqMan count (Ct) values from the end of the TaqMan PCR reaction are plotted against the known amount of synthetic target sequence to calculate a linear regression so that the amount of target DNA FAD2-1 in an unknown sample can be determined from Ct TaqMan values at the end of each Taiman PCR reaction. The plants are transformed with pMON68540, pMON68546, or pMON80623, all of which suppress FAD2-1A (see section 3A and figure 7 for descriptions of the constructs). The total RNA is obtained from weak and transformed plants using an ABI 6100 nucleic acid preparation station. The transformed plants are homozygous third generation and have oleic acid levels greater than 50%. FAD2-1 A primers, FAD2-1 B primers, or FAD2-2A primers are added in separate TaqMan samples to the total RNA of each plant to be analyzed. Samples are analyzed in an ABI 700 sequence detection instrument using one-step ABI Prism master-mix RT-PCR chemistry. All transgenic plants suppress substantially FAD2-1 A and FAD2-1 B transcript levels. None of the transgenic plants partially reduce FAD2-2A or FAD2-2B levels. Plant-to-plant comparisons of FAD2-1 A transcript levels in weak plants determine natural variation between plants. FAD2-1 A mRNA of developed seeds is analyzed using PCR primers, which produce the probe sequence in multiple plants. Seeds with a size of 0.2 g of fresh weight are taken from four different plants with weak R2 segregants, each plant from a different line. The R2 seed group of the same size class and four different weak segregants are analyzed. The PCR reactions are carried out in triplicate and the results are normalized in comparison to the amount of 18S RNA in each sample. Plant-to-plant biological variability in FAD2-1 A transcripts is low. Three of the four samples have a normalized TaqMan count value (Ct) of approximately 65 and one of the samples has a normalized TaqMan value (Ct) of approximately 50.

EXAMPLE 28 A contiguous 200 fragment of soybean intron 1 FAD2-1 sequence (SEQ ID NO: 1) is amplified via PCR to result in PCR products including the first 200 nucleotides of SEQ ID NO: 1, starting at the 5 end 'of SEQ ID NO: 1. PCR products are cloned directly, in sense orientation, into a vector containing the promoter 7Sa 'of soybean seed and a tml 3' termination sequence, in the manner of restriction sites designed at the 5 'ends of the PCR primers. The vector is then cut with a restriction enzyme and ligated into a vector containing the CP4 EPSPS gene regulated by the FMV promoter and an E9 3 'Rubisco termination sequence. The resulting gene expression construct is used for transformation using methods such as those described herein. The fatty acid compositions are analyzed from the seed of soy lines transformed with a construct using gas chromatography as described in Example 4 to identify methyl esters of fatty acid compounds extracted from seeds. First, six Ri seeds taken from soybean plants transformed with this construct are harvested, and the fatty acid composition of each single seed is determined. Since the Ri plants of each event are segregated for the transgenes and, therefore, produce seeds with conventional soybean seed composition, as well as modified versions. Positive seeds are grouped and averaged for each event. The pooled positive averages demonstrate that the saturated fatty acid compositions are altered in the oil of the seeds of transgenic soybean lines as compared to those of the non-transformed soybean seed.

EXAMPLE 29 A fragment of 180 contiguous soybean intron 1 FAD2-1 sequence (SEQ ID NO: 1) is amplified via PCR to result in PCR products that include the first 180 nucleotides of SEQ ID NO: 1, starting at end 3 'of SEQ ID NO: 1. The PCR products are cloned directly, in sense orientation, into a vector containing the soybean 7Sa 'promoter and a tml 3' terminator sequence, as a restriction site designed at the 5 'ends of the PCR primers. The vector is then cut with a restriction enzyme and ligated into a vector containing the CP4 EPSPS gene regulated by the FMV promoter and an E9 3 'Rubisco termination sequence. The resulting gene expression construct is used for transformation using methods such as those described herein. The fatty acid compositions are analyzed from the seed of soy lines transformed with this construct using gas chromatography as described in example 4 to identify methyl esters of fatty acid compounds extracted from seeds. First, six seeds R < The seeds of soybeans transformed with this construct are harvested, and the fatty acid composition of each single seed is determined. Since the R1 plants of each event are segregated for the transgenes and, therefore, produce seeds with conventional soybean seed composition, as well as modified versions. The positive seeds are group and average for each event. The pooled positive averages demonstrate that the saturated fatty acid compositions are altered in the oil of the seeds of transgenic soybean lines as compared to those of the non-transformed soybean seed.

EXAMPLE 30 pMON97562 contains a soybean 7Sa 'promoter operably linked to a soybean FAD2-1 A intron 1 (SEQ ID NO: 1), which is reduced by 100 contiguous nucleotides from the 3' end and linked to FAD3-1 A 5'UTR, followed by an FAD3-1 A 3'UTR, linked to an FAD3-1 B 5'UTR, followed by an FAD3-1 B 3'UTR, followed by a FATB-1 to 5'UTR, followed by a FATB-1 to 3'UTR, operably linked to 70 nucleotides of intron 4 FAD3-1 A, operably linked to a FATB-1 to 3 'UTR in the anti-sense orientation followed by a FATB-1a 5'UTR in orientation antisense, linked to a FAD3-1 B 3'UTR in antisense, followed by an FAD3-1 B 5'UTR in antisense, linked to an FAD3-1 A 3'UTR in antisense, followed by FAD3-1 A 5'UTR in antisense, followed by an intron 1 FAD2-1 A (SEQ ID NO: 1), which is reduced by 100 contiguous nucleotides of the 3 'end and in the antisense orientation, operably linked to a 3' H6 polyadenylation segment with a gene CP4 EPSPS operably and an EFMV promoter and an E9 3 'Rubisco termination sequence of whole pea flanked by RB and LB in the same DNA molecule. The construct of Resulting gene expression is used for soybean transformation using methods as described herein. The fatty acid compositions are determined from the seed of soybean lines transformed with this construct using gas chromatography as described in Example 4. Table 25 provides the representative seed compositions. The level of 18: 3 is reduced to approximately 1%.

TABLE 25 Fatty acid composition of single seeds R1 of events PMON97562 EXAMPLE 31 pMON97563 contains a soybean 7Sa 'promoter operably linked to a soybean FAD2-1 A intron 1 (SEQ ID NO: 1), which is reduced by 100 contiguous nucleotides of the 3' end and ligated to FAD3-1 A 5'UTR, followed by an FAD3-1 A 3'UTR, linked to an FAD3-1 B 5'UTR, followed by an FAD3-1 B 3'UTR, linked to an FAD3-1 C 5'UTR, followed by an FAD3-1 C 3'UTR, followed by a CTP coding region, followed by a CTP FATB-2a coding region operably bound to 70 nucleotides of intron 4 FAD3-1 A, operably linked to a CTP coding region FATB-2a in the antisense orientation followed by a CTP coding region FATB-1 a in the antisense orientation, linked to an FAD3-1 C 3'UTR in antisense, followed by a FAD3-1 C 5'UTR in antisense, linked to an FAD3-1 B 3'UTR in antisense, followed by FAD3-1 B 5'UTR in antisense, bound to an FAD3-1 A 3'UTR in antisense, followed by an FAD3-1 A 5 'UTR in antisense, followed by an intron 1 FAD2-1A of soybean seed (SEQ ID NO: 1), which is reduced by 100 contiguous nucleotides of the 3 end and in the antisense orientation, operably linked to a 3 H6 polyadenylation segment with a CP4 EPSPS gene operably linked to an EFMV promoter and a 3 'Rubisco E9 termination sequence of whole pea flanked by RB and LB in the same molecule. DNA The resulting gene expression construct is used for plant transformation using methods as described herein. The fatty acid compositions are determined from the seed of soybean lines transformed with this construct using gas chromatography as described in example 4. Table 26 provides the representative seed compositions. The level of 18: 3 is reduced to approximately 1%.

TABLE 26 Fatty acid composition of single seeds R1 of events PMON97563 All compositions and / or methods described and claimed herein may be made and executed without undue experimentation in view of the present disclosure. Although the compositions and methods of this invention have been described in terms of preferred embodiments, it will be apparent to those skilled in the art that variations can be applied to the compositions and / or methods and in the steps or sequence of steps of the method described herein without departing from the concept, essence and scope of the invention. More specifically, it will be apparent that certain agents that are chemically and physiologically related can be substituted for the agents described herein while the same or results are achieved. All the Similar substitutes and apparent modifications for those skilled in the art are considered within the essence, scope and concept of the invention as defined by the appended claims.

Claims

NOVELTY OF THE INVENTION CLAIMS

1 .- A soybean seed exhibiting a fatty acid composition of seed oil comprising an oleic acid content of about 42% to about 85% by weight of the total fatty acids and a saturated fatty acid content of less than 8% by weight of total fatty acids.

2. The soybean seed according to claim 1, further characterized in that said seed additionally comprises a genome with a nucleic acid sequence that suppresses the expression of FAD2-1 and FATB of endogenous soybean, where said acid sequence nucleic comprises a fragment of FAD2-1 intron of soybean that is between about 20 and about 420 nucleotides in length and a fragment of soybean gene FATB that is between about 40 and about 450 contiguous nucleotides in length; and said genome further comprises a nucleic acid sequence that increases the expression of one or both of beta-ketoacyl-ACP synthase IV and delta-9 desaturase.

3. The soybean seed according to claim 2, further characterized in that said fragment of intron FAD2-1 of soybean seed and said fragment of FATB of soybean are each transcribed in sense and antisense orientations, resulting in RNA that is at least in part double stranded.

4. - Soybean seed according to claim 2, further characterized in that said nucleic acid sequence that suppresses the expression of FAD2-1 and FATB of endogenous soybean is assembled as a functional transcription unit after insertion into a plant chromosome.

5. - The soybean seed according to claim 1, further characterized in that said seed further comprises a genome with a nucleic acid sequence comprising an FAD2-1 intron fragment of soybean seed that is between about 20 and about 420 nucleotides in length and a fragment of soybean gene FATB which is between about 40 and about 450 contiguous nucleotides in length.

6. Soybean seed according to claim 5, further characterized in that said fragment of FAD2-1 intron of soybean seed and said FATB fragment of soybean are each transcribed in sense and antisense orientations, resulting in RNA which is at least in part double-stranded.

7. The soybean seed according to claim 5, further characterized in that said nucleic acid sequence is assembled as a functional transcription unit after insertion into a plant chromosome.

8. - The soybean seed according to claim 1, further characterized in that said seed additionally comprises a genome with a nucleic acid sequence that suppresses the expression of FAD2-1 from endogenous soybean seed, comprising an intron fragment FAD2-1 of soybean seed that is between about 20 and about 420 nucleotides in length; and said genome further comprises a nucleic acid sequence that increases the expression of one or both of beta-ketoacyl-ACP synthase IV and delta-9 desaturase.

9. - A method for reducing the amount of FAD2 gene deletion with respect to the amount of FAD2 gene deletion obtained by the expression of an RNAids construct having a FAD2 heterologous sequence consisting of a complete FAD2 intron or a complete FAD2 UTR, said method comprising: i) expressing a heterologous FAD2 sequence in a plant cell, wherein the heterologous FAD2 sequence is derived from an endogenous FAD2 gene in a plant cell and consists of an FAD2 intron fragment or a FAD2 UTR fragment; and ii) the deletion of an endogenous FAD2 gene with said heterologous FAD2 sequence, wherein the amount of FAD2 gene deletion is less than the amount of gene expression obtained by the expression of a heterologous FAD2 sequence consisting of the full length of a intron FAD2 or the full length of an FAD2 UTR.

10. - The method according to claim 9, further characterized in that said fragment of intron FAD2 is found between about 40 and about 320 contiguous nucleotides of a complete FAD2 intron 1 and said FAD2 UTR fragment is about 100 to about 140 contiguous nucleotides of a 3 'FAD2 complete UTR. 1 1 .- A method for altering the oily composition of a plant cell comprising: transforming a plant cell with a heterologous FAD2 sequence derived from a part of an endogenous FAD2 gene, wherein said heterologous FAD2 sequence consists of an FAD2 intron fragment or a fragment of FAD2 UTR; and the growth of said plant cell under conditions where the transcription of the heterologous FAD2 sequence is initiated, whereby the oily composition is altered in relation to a plant cell with a similar genetic background but lacking the heterologous FAD2 sequence. 12. A method for increasing the content of oleic acid and reducing the content of saturated fatty acid in a plant seed comprising: i) shortening the length of a first heterologous FAD2 sequence up to the amount of gene deletion FAD2 of a plant transformed with said first heterologous FAD2 sequence is at least partially reduced in relation to the amount of gene deletion FAD2 in a plant cell comprising a similar genetic background and a second heterologous FAD2 sequence, where the second heterologous FAD2 sequence consists of more endogenous FAD2 sequences that said first heterologous FAD2 sequence; ii) expression of a heterologous FATB sequence capable of at least partially reducing the FATB gene expression in a plant cell in relation to the suppression of FATB in a plant cell with a similar genetic background but without the heterologous FATB sequence; iii) the growth of a plant comprising a genome with said first heterologous FAD2 sequence and said heterologous FATB sequence; and iv) cultivating a plant that produces seed with a reduced saturated fatty acid content relative to the seed of a plant that has a similar genetic background but lacks said first heterologous FAD2 sequence and said heterologous FATB sequence. 13. A method for modulating the fatty acid composition of oil from a seed of a temperate oil seed culture comprising isolating a fragment of a genetic element of at least 40 nucleotides in length that is capable of suppressing the expression of a gene endogenous in the path of fatty acid synthesis; introducing said genetic element into a plant cell of a temperate oilseed crop; produce a transgenic plant; and selecting a transgenic plant seed comprising said genetic element that modulates the oil fatty acid composition of said seed. 14. - The method according to claim 13, further characterized in that said endogenous gene is two or more endogenous genes in the path of fatty acid synthesis. 15. - The method according to claim 13, further characterized in that said genetic element is selected from group consisting of antron, an exon, a DNA sequence encoding a transit peptide, a 3'-UTR, a 5'UTR, an open reading frame or a transcript of said endogenous gene in the synthesis path of fatty acid. 16. The method according to claim 13, further characterized in that said endogenous gene is selected from the group consisting of FAD2-1, FAD3, FATB, and combinations thereof. 17. - The method according to claim 13, further characterized in that said temperate oilseed crop is selected from the group consisting of soybean, canola, sunflower, peanut, corn and cotton. 18. - A soybean cell of a soybean seed exhibiting a fatty acid composition of seed oil comprising an oleic acid content of about 42% to about 85% by weight of the total fatty acids and a content of saturated fatty acid of less than 8% by weight of the total fatty acids. 19. - A recombinant nucleic acid molecule comprising a FAD2-1 intron fragment of soybean seed that is between about 20 and about 420 nucleotides in length and a fragment of soybean gene FATB that is between about 40 and approximately 450 contiguous nucleotides in length. 20. - A recombinant nucleic acid molecule that comprises a nucleic acid sequence comprising a FAD2-1 intron fragment of soybean seed that is between about 20 and about 420 nucleotides in length, a fragment of soybean gene FATB that is between about 40 to about 450 nucleotides in length; and a nucleic acid sequence that increases the expression of one or both of beta-ketoacyl-ACP synthase IV and delta-9 desaturase. twenty-one . - An unmixed seed oil comprising a fatty acid composition comprising an oleic acid content of about 42% to about 85% by weight of the total fatty acids and a saturated fatty acid content of about 8% or less in weight of total fatty acids. 22. - A soybean meal derived from a soybean seed exhibiting a fatty acid composition of seed oil comprising an oleic acid content of about 42% to about 85% by weight of the total fatty acids and a content of saturated fatty acid of less than 8% by weight of total fatty acids. 23. - A method for decreasing the linolenic acid content of a soybean seed comprising i) introducing into a soybean cell a heterologous nucleic acid molecule comprising the nucleic acid sequence of at least two members of a family FAD3 gene; ii) expressing a nucleic acid sequence of a FAD3 gene capable of at least partially reducing endogenous FAD3 gene expression in a cell vegetable; I¡) the development of a plant cell comprising a genome with said nucleic acid sequence of at least two members of the FAD3 gene family; and iv) cultivating said plant cell with reduced content of linolenic acid in relation to a plant cell having a similar genetic background but lacking said at least two members of said FAD3 gene family. 24. The method according to claim 23, further characterized in that said gene family is selected from the group consisting of FAD3-1A, FAD3-1 B, and FAD3-1 C. 25.- The method according to the claim 23, further characterized in that said DNA fragments are 5 'or 3' -UTR of a transcribed region of FAD3. 26.- A recombinant DNA construct comprising DNA fragments of at least two members of a FAD3 gene family. 27. The recombinant DNA construct according to claim 26, further characterized in that it additionally comprises an FAD2-1 intron fragment of soybean seed that is between about 20 and about 420 contiguous nucleotides in length and a gene fragment of FATB soybean seed that is between approximately 40 and approximately 450 contiguous nucleotides in length. 28.- A non-mixed seed oil comprising a fatty acid composition comprising an oleic acid content of about 42% to about 85% by weight of the total fatty acids, a saturated fatty acid content of about 8% or less by weight of total fatty acids, and a linolenic acid content of about 1.5% or less by weight of the total fatty acids. 29. A soybean seed comprising the recombinant DNA construct of claim 26. 30.- A soybean meal derived from the soybean seed of claim 29.