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MA44418B1 - Procédé permettant la mesure quantitative de contenu particulaire à l'aide d'une imagerie à l'état hydraté - Google Patents

Procédé permettant la mesure quantitative de contenu particulaire à l'aide d'une imagerie à l'état hydraté

Info

Publication number
MA44418B1
MA44418B1 MA44418A MA44418A MA44418B1 MA 44418 B1 MA44418 B1 MA 44418B1 MA 44418 A MA44418 A MA 44418A MA 44418 A MA44418 A MA 44418A MA 44418 B1 MA44418 B1 MA 44418B1
Authority
MA
Morocco
Prior art keywords
state imaging
particulate content
quantitative measurement
hydrated state
sample
Prior art date
Application number
MA44418A
Other languages
English (en)
Other versions
MA44418A1 (fr
Inventor
Lars Haag
Martin Ryner
Mathieu Colomb-Delsuc
Rickard Nordström
Original Assignee
Intelligent Virus Imaging Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Intelligent Virus Imaging Inc filed Critical Intelligent Virus Imaging Inc
Publication of MA44418A1 publication Critical patent/MA44418A1/fr
Publication of MA44418B1 publication Critical patent/MA44418B1/fr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/42—Low-temperature sample treatment, e.g. cryofixation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06V—IMAGE OR VIDEO RECOGNITION OR UNDERSTANDING
    • G06V20/00—Scenes; Scene-specific elements
    • G06V20/60—Type of objects
    • G06V20/69—Microscopic objects, e.g. biological cells or cellular parts
    • G06V20/698—Matching; Classification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14121—Viruses as such, e.g. new isolates, mutants or their genomic sequences
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14123—Virus like particles [VLP]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/14011—Parvoviridae
    • C12N2750/14111—Dependovirus, e.g. adenoassociated viruses
    • C12N2750/14151—Methods of production or purification of viral material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/2806—Means for preparing replicas of specimens, e.g. for microscopal analysis
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N2015/0038—Investigating nanoparticles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses
    • G01N2333/075—Adenoviridae

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Dispersion Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Signal Processing (AREA)
  • Biomedical Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Theoretical Computer Science (AREA)
  • Multimedia (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Analysing Materials By The Use Of Radiation (AREA)
  • Computer Vision & Pattern Recognition (AREA)

Abstract

La présente invention concerne un procédé destiné à la mesure quantitative de contenu particulaire à l'aide d'une imagerie à l'état hydraté telle qu'une cryotem. L'invention concerne également un échantillon (100) de particules pseudo-virales (vlp) ou de particules virales. De préférence, l'échantillon (100) est rapidement congelé dans un liquide cryogénique à une température cryogénique. Selon l'invention, le contenu particulaire de chaque vlp dans l'échantillon congelé à température cryogénique est obse
MA44418A 2017-03-02 2018-01-24 Procédé permettant la mesure quantitative de contenu particulaire à l'aide d'une imagerie à l'état hydraté MA44418B1 (fr)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201762465981P 2017-03-02 2017-03-02
PCT/US2018/015263 WO2018160298A1 (fr) 2017-03-02 2018-01-25 Procédé permettant la mesure quantitative de contenu particulaire à l'aide d'une imagerie à l'état hydraté

Publications (2)

Publication Number Publication Date
MA44418A1 MA44418A1 (fr) 2020-04-30
MA44418B1 true MA44418B1 (fr) 2021-11-30

Family

ID=63370196

Family Applications (1)

Application Number Title Priority Date Filing Date
MA44418A MA44418B1 (fr) 2017-03-02 2018-01-24 Procédé permettant la mesure quantitative de contenu particulaire à l'aide d'une imagerie à l'état hydraté

Country Status (17)

Country Link
US (2) US11125672B2 (fr)
EP (1) EP3589643A4 (fr)
JP (1) JP6865769B2 (fr)
KR (1) KR102149623B1 (fr)
CN (1) CN108884133A (fr)
AU (1) AU2018228346A1 (fr)
BR (1) BR112019001151A2 (fr)
CA (1) CA3015803A1 (fr)
CL (1) CL2018002809A1 (fr)
EA (1) EA037471B1 (fr)
HK (1) HK1256983A1 (fr)
IL (1) IL263737A (fr)
MA (1) MA44418B1 (fr)
MX (1) MX2019001354A (fr)
SG (1) SG11201900270YA (fr)
WO (1) WO2018160298A1 (fr)
ZA (1) ZA201900102B (fr)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MA44418B1 (fr) * 2017-03-02 2021-11-30 Intelligent Virus Imaging Inc Procédé permettant la mesure quantitative de contenu particulaire à l'aide d'une imagerie à l'état hydraté
CN112183433B (zh) * 2020-10-12 2024-02-23 水木未来(北京)科技有限公司 一种实心和空心病毒颗粒的表征量化方法
CN113009184A (zh) * 2021-04-20 2021-06-22 浙江科技学院 一种基于低共熔溶剂制备冷冻电镜检测样品的方法
WO2025262162A1 (fr) 2024-06-20 2025-12-26 F. Hoffmann-La Roche Ag Procédé de détermination de particules d'aav recombinantes vides, partiellement remplies et pleines

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005035743A1 (fr) * 2003-10-15 2005-04-21 Vector Gene Technology Company Ltd. Procede de production, d'isolation, de purification et utilisations de vecteurs virus adeno-associes recombinants multi-types
US8712140B2 (en) * 2005-04-15 2014-04-29 Intelligent Virus Imaging Inc. Method of analyzing cell structures and their components
WO2008150588A1 (fr) * 2007-05-30 2008-12-11 Intelligent Virus Imaging Inc. Procédé pour compter et segmenter des particules virales dans une image
US20130191037A1 (en) 2011-07-26 2013-07-25 Nanoimaging Services Characterization of particulates using electron microscopy and image processing methods
CA2916827C (fr) * 2013-07-08 2021-11-02 Novartis Ag Particules de rotavirus a proteines de surface chimeres
ES2705574T3 (es) * 2013-10-22 2019-03-26 Hoffmann La Roche Procedimientos para medir partículas de virus libres de células a partir de gotas de sangre seca
US10128078B2 (en) * 2014-01-29 2018-11-13 Japan Science And Technology Agency Protective agent for electron microscopic observation of biological sample in water-containing state, kit for electron microscopic observation, methods for observation, diagnosis, evaluation, and quantification by electron microscope, and sample stage
CN104537713B (zh) * 2015-01-05 2017-10-03 清华大学 一种新型三维重构系统
MA44418B1 (fr) * 2017-03-02 2021-11-30 Intelligent Virus Imaging Inc Procédé permettant la mesure quantitative de contenu particulaire à l'aide d'une imagerie à l'état hydraté

Also Published As

Publication number Publication date
MX2019001354A (es) 2019-06-03
CN108884133A (zh) 2018-11-23
AU2018228346A1 (en) 2018-10-04
KR20190038541A (ko) 2019-04-08
CL2018002809A1 (es) 2019-01-18
KR102149623B1 (ko) 2020-08-28
WO2018160298A1 (fr) 2018-09-07
MA44418A1 (fr) 2020-04-30
US20190331584A1 (en) 2019-10-31
ZA201900102B (en) 2019-09-25
HK1256983A1 (zh) 2019-10-11
US11125672B2 (en) 2021-09-21
BR112019001151A2 (pt) 2019-04-30
EP3589643A1 (fr) 2020-01-08
JP2020513230A (ja) 2020-05-14
EA201891845A1 (ru) 2020-03-02
SG11201900270YA (en) 2019-02-27
US20210341372A1 (en) 2021-11-04
EP3589643A4 (fr) 2020-12-16
EA037471B1 (ru) 2021-03-31
JP6865769B2 (ja) 2021-04-28
CA3015803A1 (fr) 2018-09-02
IL263737A (en) 2019-01-31

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