Rice, 2007 - Google Patents
Beyond the diffraction limit: far-field fluorescence imaging with ultrahigh resolutionRice, 2007
View PDF- Document ID
- 2350270739902496424
- Author
- Rice J
- Publication year
- Publication venue
- Molecular BioSystems
External Links
Snippet
Fluorescence microscopy is an important and extensively utilised tool for imaging biological systems. However, the image resolution that can be obtained has a limit as defined through the laws of diffraction. Demand for improved resolution has stimulated research into …
- 238000000799 fluorescence microscopy 0 title abstract description 76
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
- G01N21/6458—Fluorescence microscopy
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
- G01N2021/653—Coherent methods [CARS]
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/636—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited using an arrangement of pump beam and probe beam; using the measurement of optical non-linear properties
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/002—Scanning microscopes
- G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
- G02B21/0052—Optical details of the image generation
- G02B21/0076—Optical details of the image generation arrangements using fluorescence or luminescence
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
- G02B21/00—Microscopes
- G02B21/16—Microscopes adapted for ultra-violet illumination; Fluorescence microscopes
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS, OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
- G02B21/08—Condensers
- G02B21/10—Condensers affording dark-field illumination
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Weisenburger et al. | Light microscopy: an ongoing contemporary revolution | |
| Jouchet et al. | Nanometric axial localization of single fluorescent molecules with modulated excitation | |
| Liu et al. | Breaking the Axial Diffraction Limit: A Guide to Axial Super‐Resolution Fluorescence Microscopy | |
| Blom et al. | Stimulated emission depletion microscopy | |
| Hauser et al. | Correlative super-resolution microscopy: new dimensions and new opportunities | |
| Hell | Toward fluorescence nanoscopy | |
| US7675045B1 (en) | 3-dimensional imaging at nanometer resolutions | |
| Schermelleh et al. | A guide to super-resolution fluorescence microscopy | |
| CN115753717B (en) | A fluorescence modulation multicolor super-resolution microscopy imaging method with single wavelength excitation | |
| Diaspro et al. | Optical nanoscopy | |
| Züchner et al. | Light microscopy with doughnut modes: a concept to detect, characterize, and manipulate individual nanoobjects | |
| Navratil et al. | Chemical microscopy applied to biological systems | |
| Heintzmann et al. | Breaking the resolution limit in light microscopy | |
| Patterson | Fluorescence microscopy below the diffraction limit | |
| Petibois | Imaging methods for elemental, chemical, molecular, and morphological analyses of single cells | |
| Rice | Beyond the diffraction limit: far-field fluorescence imaging with ultrahigh resolution | |
| Haustein et al. | Trends in fluorescence imaging and related techniques to unravel biological information | |
| Ginsberg et al. | Interferometric scattering microscopy | |
| Castelletto et al. | Color centers in wide-bandgap semiconductors for subdiffraction imaging: a review | |
| Li et al. | Pulsed saturated absorption competition microscopy on nonbleaching nanoparticles | |
| Eberle et al. | Super-resolution microscopy techniques and their potential for applications in radiation biophysics | |
| Radmacher et al. | Molecular Level Super-Resolution Fluorescence Imaging | |
| Klementieva et al. | The principles of super-resolution fluorescence microscopy | |
| Hedde et al. | Optical imaging of nanoscale cellular structures | |
| Owen et al. | Super-resolution imaging by localization microscopy |