Kotnik et al., 2000 - Google Patents
Evaluation of cell membrane electropermeabilization by means of a nonpermeant cytotoxic agentKotnik et al., 2000
View PDF- Document ID
- 2308587631473460139
- Author
- Kotnik T
- Maček-Lebar A
- Miklavčič D
- Mir L
- Publication year
- Publication venue
- Biotechniques
External Links
Snippet
For the evaluation of cell membrane electropermeabilization, cells are usually exposed to electric pulses in the presence of propidium iodide, a fluorescent dye activated by binding to cellular DNA. The fraction of permeabilized cells is then determined using a flow cytometer …
- 238000004520 electroporation 0 title abstract description 20
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
- G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
- G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
- G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
- G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
- G01N33/5047—Cells of the immune system
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES OR MICRO-ORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving viable micro-organisms
- C12Q1/025—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions involving viable micro-organisms for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kotnik et al. | Evaluation of cell membrane electropermeabilization by means of a nonpermeant cytotoxic agent | |
| US6261815B1 (en) | Method of introducing exogenous compounds into cells by electroporation and apparatus for same | |
| Weaver et al. | Electroporation: high frequency of occurrence of a transient high-permeability state in erythrocytes and intact yeast | |
| Castedo et al. | Sequential acquisition of mitochondrial and plasma membrane alterations during early lymphocyte apoptosis | |
| Klenchin et al. | Electrically induced DNA uptake by cells is a fast process involving DNA electrophoresis | |
| US6740497B2 (en) | Method and apparatus for detecting cancerous cells using molecules that change electrophoretic mobility | |
| Barbier et al. | Stimulation of Ca2+ influx in rat pituitary cells under exposure to a 50 Hz magnetic field | |
| Russo-Marie et al. | Beta-galactosidase activity in single differentiating bacterial cells. | |
| Prausnitz et al. | Quantitative study of molecular transport due to electroporation: uptake of bovine serum albumin by erythrocyte ghosts | |
| Nishiyama et al. | Brd4 is required for recovery from antimicrotubule drug-induced mitotic arrest: preservation of acetylated chromatin | |
| Guignet et al. | Suspended-drop electroporation for high-throughput delivery of biomolecules into cells | |
| Chien et al. | Photostick: a method for selective isolation of target cells from culture | |
| Al-Ali et al. | High content screening with primary neurons | |
| Kopp et al. | Liver-on-chip model and application in predictive genotoxicity and mutagenicity of drugs | |
| JP2017508452A (en) | System for producing host cells having artificial endosymbiosis | |
| Gabriel et al. | Spatial compartmentation and time resolution of photooxidation of a cell membrane probe in electropermeabilized Chinese hamster ovary cells | |
| Wright et al. | Susceptibility of common marmosets (Callithrix jacchus) to oncogenic and attenuated strains of Herpesvirus saimiri | |
| Dahm et al. | Visualizing mRNA localization and local protein translation in neurons | |
| Plášek et al. | Interaction of the fluorescent probe 1, 6-diphenyl-1, 3, 5-hexatriene with biomembranes | |
| Chang et al. | Electroporation and electrofusion using a pulsed radio-frequency electric field | |
| Audiffred et al. | Characterization and applications of serum‐free induced adhesion in jurkat suspension cells | |
| Matt et al. | Ex vivo analysis of DNA repair capacity of human peripheral blood mononuclear cells by a modified host cell reactivation assay | |
| Zimmermann et al. | Electroinjection and electrofusion in hypo-osmolar solution | |
| Vishwanath et al. | A quantitative high-throughput chemotaxis assay using bioluminescent reporter cells | |
| Cemazar et al. | Construction of EGFP expressing HepG2 cell line using electroporation |