Kumar et al., 2021 - Google Patents
Mechanoporation: Toward single cell approachesKumar et al., 2021
- Document ID
- 12049728018266869953
- Author
- Kumar A
- Mohan L
- Shinde P
- Chang H
- Nagai M
- Santra T
- Publication year
- Publication venue
- Handbook of Single-Cell Technologies
External Links
Snippet
A cell is an independent functioning unit, and it regulates its input and output based on its own genetic makeup omitting any external transcriptions. Unlike bulk measurement with millions of cells together, which provides an average data, single-cell analysis (SCA) can …
- 238000004458 analytical method 0 abstract description 6
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/89—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using micro-injection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kumar et al. | Mechanoporation: Toward single cell approaches | |
| Rad et al. | A comprehensive review on intracellular delivery | |
| Hur et al. | Microfluidic and nanofluidic intracellular delivery | |
| US9725709B2 (en) | Intracellular delivery and transfection methods and devices | |
| Sun et al. | Recent advances in micro/nanoscale intracellular delivery | |
| Meacham et al. | Physical methods for intracellular delivery: practical aspects from laboratory use to industrial-scale processing | |
| Stewart et al. | In vitro and ex vivo strategies for intracellular delivery | |
| CN107002089B (en) | Destruction and Field-Achieved Delivery of Compounds and Compositions into Cells | |
| JP6839073B2 (en) | Efficient delivery of large cargo to cells on a porous support | |
| Kaladharan et al. | Microfluidic based physical approaches towards single-cell intracellular delivery and analysis | |
| CN103981090B (en) | Gene imports chip and method of gene introduction | |
| Qu et al. | Surface-mediated intracellular delivery by physical membrane disruption | |
| US20160108433A1 (en) | Systems, apparatus, and methods for droplet-based microfluidics cell poration | |
| CN108531396B (en) | A microfluidic chip for cell transfection | |
| EP3580556B1 (en) | Device and method for intracellular delivery of biomolecular cargo via acoustic wave exposure | |
| WO2020132470A1 (en) | Systems and methods for mitigating particle aggregation caused by standing wave and transient acoustophoretic effects | |
| Huang et al. | Continuous vector-free gene transfer with a novel microfluidic chip and nanoneedle array | |
| WO2002058847A9 (en) | Cell transformation using a single chip silicon microfabricated array incorporating integrated micro-piercing injectors | |
| Ji et al. | Physical intracellular delivery based on microfluidic technology | |
| CN111133314B (en) | Efficient delivery of cargo across cell membranes using a continuous flow fluidics system | |
| Park et al. | Intracellular delivery of molecules using microfabricated nanoneedle arrays | |
| WO2007053802A2 (en) | Drug and gene delivery by polymer nanonozzle and nanotip cell patch | |
| Huang et al. | Ultrasound-mediated plasmid delivery by stable oscillation of linear array microbubbles in microfluidic channels | |
| Dong et al. | Advanced microfluidic devices for cell electroporation and manipulation | |
| Mao et al. | Mechanically mediated cargo delivery to cells using microfluidic devices |