Einhauer et al., 2001 - Google Patents
The FLAG™ peptide, a versatile fusion tag for the purification of recombinant proteinsEinhauer et al., 2001
- Document ID
- 11318155028161961422
- Author
- Einhauer A
- Jungbauer A
- Publication year
- Publication venue
- Journal of biochemical and biophysical methods
External Links
Snippet
A fusion tag, called FLAG™ and consisting of eight amino acids ( AspTyrLysAspAspAspAspLys) including an enterokinase-cleavage site, was specifically designed for immunoaffinity chromatography. It allows elution under non-denaturing …
- 230000004927 fusion 0 title abstract description 40
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/1072—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides by covalent attachment of residues or functional groups
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/04—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length on carriers
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/44—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material not provided for elsewhere, e.g. haptens, metals, DNA, RNA, amino acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICRO-ORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING OR MAINTAINING MICRO-ORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1062—Isolating an individual clone by screening libraries mRNA-Display, e.g. polypeptide and encoding template are connected covalently
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by the preceding groups
- G01N33/48—Investigating or analysing materials by specific methods not covered by the preceding groups biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
- G01N33/6842—Proteomic analysis of subsets of protein mixtures with reduced complexity, e.g. membrane proteins, phosphoproteins, organelle proteins
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Einhauer et al. | The FLAG™ peptide, a versatile fusion tag for the purification of recombinant proteins | |
| Nygren et al. | Engineering proteins to facilitate bioprocessing | |
| Huse et al. | Purification of antibodies by affinity chromatography | |
| Young et al. | Recombinant protein expression and purification: a comprehensive review of affinity tags and microbial applications | |
| JP6856938B2 (en) | Novel immunoglobulin binding proteins and their use in affinity purification | |
| Hochuli et al. | Genetic approach to facilitate purification of recombinant proteins with a novel metal chelate adsorbent | |
| Walls et al. | Tagging recombinant proteins to enhance solubility and aid purification | |
| Pentelute et al. | Selective desulfurization of cysteine in the presence of Cys (Acm) in polypeptides obtained by native chemical ligation | |
| Sheibani | Prokaryotic gene fusion expression systems and their use in structural and functional studies of proteins | |
| Costa et al. | Fusion tags for protein solubility, purification and immunogenicity in Escherichia coli: the novel Fh8 system | |
| Kimple et al. | Overview of affinity tags for protein purification | |
| Berdichevsky et al. | Matrix-assisted refolding of single-chain Fv–cellulose binding domain fusion proteins | |
| US20090220455A1 (en) | Pharmaceutical compositions comprising elp fusion proteins | |
| Nilsson et al. | Fusion proteins in biotechnology and structural biology | |
| Nilsson et al. | Multiple affinity domains for the detection, purification and immobilization of recombinant proteins | |
| CN117736281A (en) | Alkaline stable FC binding proteins for affinity chromatography | |
| WO1996013583A2 (en) | Targeted hetero-association of recombinant proteins to multi-functional complexes | |
| WO2002074928A2 (en) | Fusion peptides isolatable by phase transition | |
| WO2002066514A3 (en) | Artificial fusion proteins with reduced immunogenicity | |
| Amarasinghe et al. | The use of affinity tags to overcome obstacles in recombinant protein expression and purification | |
| US7799561B2 (en) | Affinity peptides and method for purification of recombinant proteins | |
| Loughran et al. | Tagging recombinant proteins to enhance solubility and aid purification | |
| CN114829379B (en) | Binding protein of enzyme acid alpha glucosidase (GAA) and its use | |
| CN113348176A (en) | Immunoglobulin-binding proteins for affinity purification | |
| JP7606783B2 (en) | Immunoglobulin-binding proteins for affinity purification |