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Ohshima et al., 1995 - Google Patents

Selective release of intracellular protein using pulsed electric field

Ohshima et al., 1995

Document ID
7563942226464102808
Author
Ohshima T
Sato M
Saito M
Publication year
Publication venue
Journal of Electrostatics

External Links

Snippet

A release of intracellular protein under applied pulsed electric field conditions was studied using two yeast strains as subjects. In the present study, electric field strength was varied from 0 to 18 kV/cm. The release of protein with pulse treatment from the yeast cell having a …
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
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    • C12N1/00—Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor
    • C12N1/005—Micro-organisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving micro-organisms or compositions thereof; Processes of preparing or isolating a composition containing a micro-organism; Culture media therefor after treatment of microbial biomass not covered by C12N1/02 - C12N1/08
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    • C12N9/14—Hydrolases (3)
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    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
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